LOCUS       sequence-45070-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Mark WY, Liao JC, Lu Y, Ayed A, Laister R, Szymczyna B, Chakrabartty
            A, Arrowsmith CH
  TITLE     Characterization of segments from the central region of BRCA1: an 
            intrinsically disordered scaffold for multiple protein-protein and 
            protein-DNA interactions?
  JOURNAL   J Mol Biol. 2005 Jan 14;345(2):275-87.
  PUBMED    15571721
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol 
            Biol."; date: "2005-01-14"; volume: "345(2)"; pages: "275-87"
COMMENT     Sequence Label: BRCA1-(360-498)
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             56..73
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             83..100
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(577..595)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      591..638
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(813..818,819..835,836..841))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(868..886)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 attccctctg aatattttgt ttactttaag aaggagatat accatgggca gcagccatca
       61 tcatcatcat cacagcagcg gcctggtgcc gcgcggcagc catatgtgct cagagaatcc
      121 tagagatact gaagatgttc cttggataac actaaatagc agcattcaga aagttaatga
      181 gtggttttcc agaagtgatg aactgttagg ttctgatgac tcacatgatg gggagtctga
      241 atcaaatgcc aaagtagctg atgtattgga cgttctaaat gaggtagatg aatattctgg
      301 ttcttcagag aaaatagact tactggccag tgatcctcat gaggctttaa tatgtaaaag
      361 tgaaagagtt cactccaaat cagtagagag taatattgaa gacaaaatat ttgggaaaac
      421 ctatcggaag aaggcaagcc tccccaactt aagccatgta actgaaaatc taattatagg
      481 agcatttgtt actgagccac agataataca agagcgtccc ctctaaggat ccggctgcta
      541 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac
      601 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg
      661 gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag
      721 ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact
      781 gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca
      841 aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta t
//
