LOCUS       sequence-450703-        8357 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid expressing the SCT1 allele from S288C, under control of its 
            native promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8357)
  AUTHORS   Paltenghi C, van Leeuwen J
  TITLE     Genetic suppression interactions are highly conserved across 
            genetically diverse yeast isolates.
  JOURNAL   G3 (Bethesda). 2025 Mar 3:jkaf047. doi: 10.1093/g3journal/jkaf047.
  PUBMED    40037589
REFERENCE   2  (bases 1 to 8357)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2025 Mar 3:jkaf047. doi: 10.1093/g3journal/jkaf047."
COMMENT     Sequence Label: pRS313-SCT1-S288C
FEATURES             Location/Qualifiers
     source          1..8357
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4549..4568)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4550..4568)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4575..4592)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4575..4591)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4584..4606)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4732..5187
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4819..4838)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5029..5050
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(5447..6109)
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(6110..6297)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     primer_bind     complement(6444..6463)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6563..6585
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6623..6641)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    6689..7192
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        7222..7326
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7327..7395,7396..8187)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7545..7564)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc
     1021 tcactaaagg gaacaaaagc tggtaccggg ccccccctaa aaagcccctg tgcgagggat
     1081 atacataaac attgagcatt aatatttaca actcccaatg agcaataatg ggtttattac
     1141 ataaatagaa cggcgaaaga tttagtttct agtatcttca ccgtcagtaa atggcatcta
     1201 cgcatctcct tctttccctt cttcttcttc ttcctcttct tcttcttcct cttcttcttc
     1261 ctcttcctct tccctggcag tattttcacc aattctcttg tttaagacgg cctgtgcgat
     1321 tttacttgct aatccatttt tttcctccaa gatttcgttt tctacctcaa attcggagga
     1381 agaagaaggt gcaacggatg tcgaagctaa ggaagactct gacttacgat gaaaagtaga
     1441 agagaataat ggaatgttag agagggaatt gtcactattg accaatgaga cgccatcgga
     1501 atcttggtta tggtgttcat aggcatcgtg gttgtcacgt tggctgatga taggtgatga
     1561 cgaatctttt tcttgtcttt ttattttctg ttttcttagc attttcctgc gattcaattc
     1621 tgaggttttt cgatcttctt cctcttcatc gatgacgtcg aattcttcac gtagggcggc
     1681 actatcgaaa tcggggaata attcgcttcc aaagttattt acaacttcga ttattctttc
     1741 tgccagattt ctacgatcct tttgtagctt ttgcaagccc tttggagatg taagagataa
     1801 aaccagtggt ctcaaagatt tgaaaccatc cataccaata tcacccacga ttaaggcgga
     1861 atacgtgact ataacacacg agatgtaaga cccggaaaaa acatatattt tattccatgg
     1921 tttatgtctg aggtaataag tgattaaaac ggaccaaaag atgtaaagca agggcgcaaa
     1981 tcccatccca atcaagattt tccacgtggc aatgacatcg ttagccttta ttttaactgt
     2041 agacttggac aaagcggtac gggccttttc ttgagaaatt ctcttggcta atatgaagac
     2101 aggtgagaac ataatgatac ctggcatggc taacgaaaag aggatgcaga gcccgatgga
     2161 tctaaaaaaa acaagtccga ggttttttgc gaaatttacc tttgcctcct ccacaaggtg
     2221 atcaggaaga ttatagtgtc tcaaggcggc attatatgcc attatatctt tggtcaagtc
     2281 cgctatttta ggatcgtttc tatagaattc gtaacctttg accattcttc tgttcatttc
     2341 cacaatcaag ggcaacggta acttggtgct aaattgtgtc atatatagtc ttcttatcgt
     2401 ttgaaccacc atcaaagttt cataatcaga acatgtaacg gtaacggatt gtaaaccctt
     2461 cgatatggta tctaataatt ctttcactgc atctctgttc gtttccgggt tgtggtactt
     2521 ggcgactagt tccttcggta tttcaatggg gtcaccgaat tcaacaaccg ctctcgacct
     2581 gaacttatgt ggatggaaat aattcatacc gcagggaaca atcttaacat tgacgtcagg
     2641 atgcttatcc atgcaaccaa gagccataat cgccacacct gctttcaggg gcaacaagtt
     2701 tgttctgtcg tgggacccac cttcaggaaa gatcccaatg cagttgttat gggccaaatg
     2761 ctcaaaaact ctatggtaaa cgcaagattg gtcgacttta gcggcatatt tataagtagt
     2821 gccggtgagt aaagcagttt taatctctgg tttggccatt ttgaactctt ttcttagggt
     2881 caaactcgtg tcactttcta tggactggat ttctccaaat cccattgatt tgggtaaccc
     2941 gatgagaccc tttggcatac aatcagtcaa gaaatgcgtg tcgtggccga taactctctt
     3001 gtagtctgtt ggatctacgc ggatagtacc ttctgccggt ttcaaattat cctgcggcct
     3061 taccacgcct atggccatga agaaactagc caaaaaccct atggggggtt gctttaatga
     3121 gctctccgca atcaagaagg acacacgtct gttgacagat ttcttcacct cgcccataag
     3181 gattacaggg tcgacgaact ggttagcatg cggagccgca acaaagatca ccggtccctg
     3241 ttgcggtacc ttgaaactgc cccggcctct gatttctcta aagaagcagt ggaagatgtt
     3301 gtacaacagc catgtggcga tggagtaaag tatcttcttg gtagcgctag gctcttggta
     3361 gatgtatgcc tgatcagccg acgtcttgac gcttttggcc tcgatggaac tgtaattcgt
     3421 agttttctgt gtgctcttgg aagaggcaaa tttctccgtg agttttggtg caggcatttt
     3481 tttttacccc gatgtaaacg cccctgtgct ctttttacta tatatttaat tccgggcttc
     3541 ctacacttaa ttcactctgt tatgaactac aagccattac taacatcaat ctttccgaat
     3601 cataaataaa ctgcaactgg gaaagaaggc gggcctactt ttctttttct tcatagcgcg
     3661 tagggcttgg cagccatagt tgttgagaga ccgatggatg agggattgaa agaagggaga
     3721 agaagaatac taaaagagga atgtagagcc gggttacagg aaaactaact tttcactcag
     3781 ggcgccgcat cttttccgca ggcgcgccat gaactggcca gcacgttcta cggcgaaacc
     3841 ttcgaactgg aggcggctca cacatagtag taagttgccg cgacaggtga ccaaaataca
     3901 gtctttaata tcatcatatt tgaacatgcc cggtttactg ccattatcta gacgtgcgtc
     3961 tctcatcact ttacactcat gaaacaatat tcgttttaat actgaatttt gagcgtcctt
     4021 tcttgccgct gtcgcttctt tgaacgcgtg cagaggaccg agcgtttcga gtttgttaag
     4081 tagttcggcg gcagaatcgc gcgcccaatg aatgcgttta tcttctgttg ttatacggtg
     4141 ggcgtaactg ggtttgtaag ctgttggcgc ttgcacacgg ttctgtggta gatataacct
     4201 ctcacgaagc gtttggccca aaagttgggc gccaagggcg cccaactggt ccatcagtat
     4261 ggcagtcctc cgaggcaggc cctcagaatt aaaatctgcc gtcgaatcat tgactctccc
     4321 ttttgacagc attgttgcga tcgccagtgg ctccgtctgc gctacaattg caccatggtc
     4381 aaaccgatcc ggatgcagtg tctgtatagt tacaccggtg taagtgtcac cctcgagcag
     4441 agctcgctgg ataggtgcac tgcctttatg tctgggaagt agcgaaggat ggacgtttag
     4501 cgcccactag ttctagagcg gccgccaccg cggtggagct ccaattcgcc ctatagtgag
     4561 tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     4621 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     4681 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gacgcgccct
     4741 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     4801 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     4861 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     4921 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     4981 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     5041 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     5101 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     5161 ttaacaaaat attaacgctt acaatttcct gatgcggtat tttctcctta cgcatctgtg
     5221 cggtatttca caccgcatag atccgtcgag ttcaagagaa aaaaaaagaa aaagcaaaaa
     5281 gaaaaaagga aagcgcgcct cgttcagaat gacacgtata gaatgatgca ttaccttgtc
     5341 atcttcagta tcatactgtt cgtatacata cttactgaca ttcataggta tacatatata
     5401 cacatgtata tatatcgtat gctgcagctt taaataatcg gtgtcactac ataagaacac
     5461 ctttggtgga gggaacatcg ttggtaccat tgggcgaggt ggcttctctt atggcaaccg
     5521 caagagcctt gaacgcactc tcactacggt gatgatcatt cttgcctcgc agacaatcaa
     5581 cgtggagggt aattctgcta gcctctgcaa agctttcaag aaaatgcggg atcatctcgc
     5641 aagagagatc tcctactttc tccctttgca aaccaagttc gacaactgcg tacggcctgt
     5701 tcgaaagatc taccaccgct ctggaaagtg cctcatccaa aggcgcaaat cctgatccaa
     5761 acctttttac tccacgcacg gcccctaggg cctctttaaa agcttgaccg agagcaatcc
     5821 cgcagtcttc agtggtgtga tggtcgtcta tgtgtaagtc accaatgcac tcaacgatta
     5881 gcgaccagcc ggaatgcttg gccagagcat gtatcatatg gtccagaaac cctatacctg
     5941 tgtggacgtt aatcacttgc gattgtgtgg cctgttctgc tactgcttct gcctcttttt
     6001 ctgggaagat cgagtgctct atcgctaggg gaccaccctt taaagagatc gcaatctgaa
     6061 tcttggtttc atttgtaata cgctttacta gggctttctg ctctgtcatc tttgccttcg
     6121 tttatcttgc ctgctcattt tttagtatat tcttcgaaga aatcacatta ctttatataa
     6181 tgtataattc attatgtgat aatgccaatc gctaagaaaa aaaaagagtc atccgctagg
     6241 tggaaaaaaa aaaatgaaaa tcattaccga ggcataaaaa aatatagagt gtactagagg
     6301 aggccaagag taatagaaaa agaaaattgc gggaaaggac tgtgttatga cttccctgac
     6361 taatgccgtg ttcaaacgat acctggcagt gactcctagc gctcaccaag ctcttaaaac
     6421 gggaatttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     6481 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     6541 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     6601 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     6661 ataataatgg tttcttaggg tccttttcat cacgtgctat aaaaataatt ataatttaaa
     6721 ttttttaata taaatatata aattaaaaat agaaagtaaa aaaagaaatt aaagaaaaaa
     6781 tagtttttgt tttccgaaga tgtaaaagac tctaggggga tcgccaacaa atactacctt
     6841 ttatcttgct cttcctgctc tcaggtatta atgccgaatt gtttcatctt gtctgtgtag
     6901 aagaccacac acgaaaatcc tgtgatttta cattttactt atcgttaatc gaatgtatat
     6961 ctatttaatc tgcttttctt gtctaataaa tatatatgta aagtacgctt tttgttgaaa
     7021 ttttttaaac ctttgtttat ttttttttct tcattccgta actcttctac cttctttatt
     7081 tactttctaa aatccaaata caaaacataa aaataaataa acacagagta aattcccaaa
     7141 ttattccatc attaaaagat acgaggcgcg tgtaagttac aggcaagcga tccgtcaggt
     7201 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     7261 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     7321 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     7381 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     7441 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     7501 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     7561 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     7621 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     7681 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     7741 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     7801 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     7861 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     7921 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     7981 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     8041 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     8101 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     8161 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     8221 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     8281 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     8341 aagatcaaag gatcttc
//
