LOCUS       sequence-45071-e         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Mark WY, Liao JC, Lu Y, Ayed A, Laister R, Szymczyna B, Chakrabartty
            A, Arrowsmith CH
  TITLE     Characterization of segments from the central region of BRCA1: an 
            intrinsically disordered scaffold for multiple protein-protein and 
            protein-DNA interactions?
  JOURNAL   J Mol Biol. 2005 Jan 14;345(2):275-87.
  PUBMED    15571721
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol 
            Biol."; date: "2005-01-14"; volume: "345(2)"; pages: "275-87"
COMMENT     Sequence Label: BRCA1-(408-540)
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             60..77
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             87..104
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(563..581)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      577..624
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(799..804,805..821,822..827))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(854..872)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcgaattccc tctgaatatt ttgtttactt taagaaggag atataccatg ggcagcagcc
       61 atcatcatca tcatcacagc agcggcctgg tgccgcgcgg cagccatatg aaagtagctg
      121 atgtattgga cgttctaaat gaggtagatg aatattctgg ttcttcagag aaaatagact
      181 tactggccag tgatcctcat gaggctttaa tatgtaaaag tgaaagagtt cactccaaat
      241 cagtagagag taatattgaa gacaaaatat ttgggaaaac ctatcggaag aaggcaagcc
      301 tccccaactt aagccatgta actgaaaatc taattatagg agcatttgtt actgagccac
      361 agataataca agagcgtccc ctcacaaata aattaaagcg taaaaggaga cctacatcag
      421 gccttcatcc tgaggatttt atcaagaaag cagatttggc agttcaaaag actcctgaaa
      481 tgataaatca gggaactaac caaacggagt aaggatccgg ctgctaacaa agcccgaaag
      541 gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct
      601 aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tcccgcaaga
      661 ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga
      721 ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta
      781 actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct
      841 tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
      901 gtttcttaga cgtcaggtgg c
//
