LOCUS       sequence-450717-       22225 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-compatible Level P vector for improved autonomous 
            bioluminescence in plants encoding kanamycin resistance cassette, 
            mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 22225)
  AUTHORS   Shakhova ES, Karataeva TA, Markina NM, Mitiouchkina T, Palkina KA, 
            Perfilov MM, Wood MG, Hoang TT, Hall MP, Fakhranurova LI, Alekberova
            AE, Malyshevskaia AK, Gorbachev DA, Bugaeva EN, Pletneva LK, Babenko
            VV, Boldyreva DI, Gorokhovatsky AY, Balakireva AV, Gao F, Choob VV, 
            Encell LP, Wood KV, Yampolsky IV, Sarkisyan KS, Mishin AS
  TITLE     An improved pathway for autonomous bioluminescence imaging in 
            eukaryotes.
  JOURNAL   Nat Methods. 2024 Mar;21(3):406-410. doi: 
            10.1038/s41592-023-02152-y. Epub 2024 Jan 22.
  PUBMED    38253843
REFERENCE   2  (bases 1 to 22225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-023-02152-y"; journalName: "Nat Methods"; date: 
            "2024-03"; volume: "21"; issue: "3"; pages: "406-410"
COMMENT     Sequence Label: pNK3071
FEATURES             Location/Qualifiers
     source          1..22225
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    15..39
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(162..750)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(242..261)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(921..1715)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCAKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     promoter        complement(1716..1820)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2103..2297
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(2363..3436)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3865..4494)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    4758..4782
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        4966..5145
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    5176..5230
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             5232..6026
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MVEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5286..5305)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5896..5915
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      6053..6760
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        6860..7205
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      12350..13057
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        16093..16438
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             complement(16999..17010)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        17931..18276
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      19294..20001
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     terminator      21918..22170
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat
       61 tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt
      121 gccaaccaca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
      181 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
      241 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
      301 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
      361 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
      421 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
      481 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
      541 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
      601 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
      661 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
      721 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
      781 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
      841 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
      901 gagtaaactt ggtctgacag ctaaaacaat tcatccagta aaatataata ttttattttc
      961 tcccaatcag gcttgatccc cagtaagtca aaaaatagct cgacatactg ttcttccccg
     1021 atatcctccc tgatcgaccg gacgcagaag gcaatgtcat accacttgtc cgccctgccg
     1081 cttctcccaa gatcaataaa gccacttact ttgccatctt tcacaaagat gttgctgtct
     1141 cccaggtcgc cgtgggaaaa gacaagttcc tcttcgggct tttccgtctt taaaaaatca
     1201 tacagctcgc gcggatcttt aaatggagtg tcctcttccc agttttcgca atccacatcg
     1261 gccagatcgt tattcagtaa gtaatccaat tcggctaagc ggctgtctaa gctattcgta
     1321 tagggacaat ccgatatgtc gatggagtga aagagcctga tgcactccgc atacagctcg
     1381 ataatctttt cagggctttg ttcatcttca tactcttccg agcaaaggac gccatcggcc
     1441 tcactcatga gcagattgct ccagccatca tgccgttcaa agtgcaggac ctttggaaca
     1501 ggcagctttc cttccagcca tagcatcatg tccttttccc gttccacatc ataggtggtc
     1561 cctttatacc ggctgtccgt catttttaaa tataggtttt cattttctcc caccagctta
     1621 tataccttag caggagacat tccttccgta tcttttgcgc agcggtattt ttcgatcagt
     1681 tttttcaatt ccggtgatat tctcatttta gccatactct tcctttttca atattattga
     1741 agcatttatc aaggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     1801 aaacaaatag gggttccgcg cacgaattgg ccagcgctgc catttttggg gtgaggccgt
     1861 tcgcggccga ggggcgcagc ccctgggggg atgggagctc ggtgccttga tgtgggcgcc
     1921 ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc
     1981 cagccatttt tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg
     2041 agcgcagcga ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca
     2101 gttatgcaca ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg
     2161 aaaaatcgcc ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt
     2221 acggctttgg gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa
     2281 tgtacgtgct atccacagga aagaagcttt ttcgaccttt ttcccctgct agggcaattt
     2341 gccctagcat ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc
     2401 ggtagcgcat gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg
     2461 gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg
     2521 cgctgccact gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg
     2581 cgcggcgtgc caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg
     2641 ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag
     2701 ctagctcgat ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc
     2761 taatcaaggc ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac
     2821 gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct
     2881 gctttccgcc atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca
     2941 cgcggccggg cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg
     3001 aaaccgccat cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg
     3061 aaacctctac gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac
     3121 gcttcgacag acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt
     3181 catagagcat cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg
     3241 acgacgcacc ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt
     3301 gccacgattc accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg
     3361 ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg
     3421 gtttgcgacc gctcacgccg attcctcggg cttgggggtt ccagtgccat tgcagggccg
     3481 gcagacaacc cagccgctta cgcctggcca accgcccgtt cctccacaca tggggcattc
     3541 cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg cctcctttag ccgctaaaat
     3601 tcatctactc atttattcat ttgctcattt actctggtag ctgcgcgatg tattcagata
     3661 gcagctcggt aatggtcttg ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct
     3721 ccgccggcaa ctgaaagttg acccgcttca tggctggcgt gtctgccagg ctggccaacg
     3781 ttgcagcctt gctgctgcgt gcgctcggac ggccggcact tagcgtgttt gtgcttttgc
     3841 tcattttctc tttacctcat taactcaaat gagttttgat ttaatttcag cggccagcgc
     3901 ctggacctcg cgggcagcgt cgccctcggg ttctgattca agaacggttg tgccggcggc
     3961 ggcagtgcct gggtagctca cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta
     4021 cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac
     4081 gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa ccagctccac
     4141 caggtcggcg gtggcccata tgtcgtaagg gcttggctgc accggaatca gcacgaagtc
     4201 ggctgccttg atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt cgatcactac
     4261 gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac
     4321 aacggttagc ggttgatctt cccgcacggc cgcccaatcg cgggcactgc cctggggatc
     4381 ggaatcgact aacagaacat cggccccggc gagttgcagg gcgcgggcta gatgggttgc
     4441 gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca gcgataacct tcatgcgttc
     4501 cccttgcgta tttgtttatt tactcatcgc atcatatacg cagcgaccgc atgacgcaag
     4561 ctgttttact caaatacaca tcaccttttt agacggcggc gctcggtttc ttcagcggcc
     4621 aagctggccg gccaggccgc cagcttggca tcagacaaac cggccaggat ttcatgcagc
     4681 cgcacggctg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg
     4741 agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac gcttagacaa
     4801 cttaataaca cattgcggac gtttttaatg tactggggtg gatgcagtgg gccccactct
     4861 gtggaagaca atgccgaatt cggatccgga gcggagaatt aagggagtca cgttatgacc
     4921 cccgccgatg acgcgggaca agccgtttta cgtttggaac tgacagaacc gcaacgttga
     4981 aggagccact gagccgcggg tttctggagt ttaatgagct aagcacatac gtcagaaacc
     5041 attattgcgc gttcaaaagt cgcctaaggt cactatcagc tagcaaatat ttcttgtcaa
     5101 aaatgctcca ctgacgttcc ataaattccc ctcggtatcc aattagagtc tcatattcac
     5161 tctcctattt ttacaacaat taccaacaac aacaaacaac aaacaacatt acaattacat
     5221 ttacaattac catggttgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     5281 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     5341 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     5401 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     5461 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     5521 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     5581 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     5641 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     5701 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc
     5761 gcatgcccga cggcgaggat ctcgtcgtga ctcatggcga tgcctgcttg ccgaatatca
     5821 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     5881 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     5941 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     6001 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgctagag tcctgcttta
     6061 atgagatatg cgagacgcct atgatcgcat gatatttgct ttcaattctg ttgtgcacgt
     6121 tgtaaaaaac ctgagcatgt gtagctcaga tccttaccgc cggtttcggt tcattctaat
     6181 gaatatatca cccgttacta tcgtattttt atgaataata ttctccgttc aatttactga
     6241 ttgtacccta ctacttatat gtacaatatt aaaatgaaaa caatatattg tgctgaatag
     6301 gtttatagcg acatctatga tagagcgcca caataacaaa caattgcgtt ttattattac
     6361 aaatccaatt ttaaaaaaag cggcagaacc ggtcaaacct aaaagactga ttacataaat
     6421 cttattcaaa tttcaaaagt gccccagggg ctagtatcta cgacacaccg agcggcgaac
     6481 taataacgct cactgaaggg aactccggtt ccccgccggc gcgcatgggt gagattcctt
     6541 gaagttgagt attggccgtc cgctctaccg aaagttacgg gcaccattca acccggtcca
     6601 gcacggcggc cgggtaaccg acttgctgcc ccgagaatta tgcagcattt ttttggtgta
     6661 tgtgggcccc aaatgaagtg caggtcaaac cttgacagtg acgacaaatc gttgggcggg
     6721 tccagggcga attttgcgac aacatgtcga ggctcagccg ctgcaagaat tcaagcttgg
     6781 aggtcaacat ggtggagcac gacactctgg tctactccaa aaatgtcaaa gatacagtct
     6841 cagaagatca aagggctatt gagacttttc aacaaaggat aatttcggga aacctcctcg
     6901 gattccattg cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaaggtggct
     6961 cctacaaatg ccatcattgc gataaaggaa aggctatcat tcaagatctc tctgccgaca
     7021 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gaggttccaa
     7081 ccacgtctac aaagcaagtg gattgatgtg acatctccac tgacgtaagg gatgacgcac
     7141 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
     7201 ggacacgctc gagtataaga gctcattttt acaacaatta ccaacaacaa caaacaacaa
     7261 acaacattac aattacattt acaattatcg atacaatgtc agcctcttca tcttattctg
     7321 aattggagtc tccaacttct cttcttgacg tgttcgtgca cgccgctcgg gaccctcaca
     7381 ccgcatcaag aagggtgttg gagtgcggat ctgacacatg gacatacgcc gccttggatg
     7441 ccgtgtctga tggtatagcc agggagttgg cccctttcgg tttggcccca aaggtcgctg
     7501 ttgtctctga gaaccatcca tttgtgttcg ctttgttgtt cgcagtctgg aagttgggtg
     7561 gaactttcat cccaatcgac gcacatgtcc cttttgccat gttgacaggt atggtgaaca
     7621 tcgtgaagcc aacatgtctt taccttcctg cctcagccac atcaaacata tctcttgtga
     7681 aggccttcga cataagaacc gtcgtgtttg gacacaagga gaattcaatg caagctttgt
     7741 tcgacaagta ctcattgcac gcagccccat tgcacgctgc accattgcat tacgcccctc
     7801 catcagccga ccatgcctgt ctttatcttt tcacttcttc agcctcttct accaaaaact
     7861 tgaaggccgt ccctcttacc catacacttg tcttgagagg ttgtcaatct aaacttgctt
     7921 ggtggaggag ggtccaacca ggaaagaatc ttgacgctat cagaatcctt ggatgggcac
     7981 catggtccca cgttcttgcc tatatgcagg atatcggaac agctacactt cttaacgccg
     8041 gttgctacgt ctttgcaacc atcccttctt cataccctca gcttcctacc actaccccag
     8101 tcgatttgac cacttcactt atcgacgctt tggtcaccag aagagtcgca gcattcgcct
     8161 gtgtcccttt cgtccttgcc aaccttaaag ccgcttgcca gtcaaaccac ccagccaggt
     8221 cacagttgct tgaggccctt caaaagacca tcatgttgga gtgcggtggt gccgtgttgg
     8281 acgacgctac tgtggactgg gccgagagga acggtatcag gatcttcacc ggaatcggta
     8341 tgaccgagac tggtggagcc gttttcgtcg gtttggcctc tgaatctaga agaggatttt
     8401 tgcctgaagg tttgcttggt gatgcatcat tctctatctc atctgacact gacgctcttg
     8461 acgaaggtga acttgtcgtg aaatcaaaat tgatcgcacc aggatatgtc ggatacgacg
     8521 acggagccca ctctgtggac tgcgacggat gggtcacatt caggacagga gataggtata
     8581 ggcagaccca gccagacgga aggtttacct ggttgggaag gatcacagac ttcattcaga
     8641 tggtgtctgg tgagtacttg gatccaaggc cattggagga gtctcttaga gcctctccat
     8701 tgatcgccaa cgcctgcatc gtgggtgacg ccttcctatc ttctgcctca acttcaatct
     8761 tggccatcat cgagttggca acccctgatc ttgcacacac cgcctcaatc agggcacagc
     8821 ttgcaagagt ccttgcccct cttaacaggg atttgcctcc accacttagg atcgcagcat
     8881 cttcaatcct tgttttggac ggaatgagaa agatccctaa gacaaagaag ggtgacatct
     8941 tcagaaaaaa gcttgaggac acattcggtg ctgagtttga gcagatgttg agaaccgaga
     9001 aagtcggatt gggtgacttg gctgatgtgg atgccggtat cactagaatc atcggaaact
     9061 tgttgggaat ctcagacgac gagcttcttt ctaccatgtc ttttgctgag ttgggtatga
     9121 cctctctttt ggccgtcaag atcgcatcag aattgaacaa gtttttggac ggtagggctg
     9181 tgttgccaac aaacatctgt tacatacact ttgacgtgcc ttcattgact tcatcagtca
     9241 gagaaaggct ttcttctgca ccatcatcta tcacatctgc cgcctctgag cctgcagcct
     9301 cttcaccagg aacccctagg gccgacgaaa tcgtgatcgt gggaaaggcc ttcagacttc
     9361 ctgacggtgt gaacgacgac gccgctttgt gggacgtcct taccggtgag tctgcatcta
     9421 tcatcaagga catcccagcc gacagatggg accacgcctc tttctaccca aaggacatcc
     9481 actttgggag agctggtctt gtggatgtcg ctagattcga ctacggtttt ttcggaatga
     9541 ccgcttctga ggcatattct ctttcaccaa ccatgaggtt ggctcttgaa gtcgcttatg
     9601 aggccttgga ggacgcaaac attcctttca gagccgtcaa gggttcaagg atgggtgtgt
     9661 tcgtcgctgt gaaagacgat ggattcgaaa ccttgttgca tgcaggacag ggttatgacg
     9721 cttacacaag attctatgga actggaaggg ctccttcaac cgcctctggt agaatcaact
     9781 atttgcttga tttgcacgga ccttcaatca ccgtcgacac agcctgctca ggaggaatcg
     9841 tgtgcatcga ccaggctgtc acctatcttc aatcaggagc tgcagagact gccatcgtgt
     9901 gttcatctaa cacacactgc tggccaggat ctttcatgtt cttgaccgct caaggaatgg
     9961 cctcacctaa cggtagatgc gcatctttca catcagacgc cgacggatac gctccttctg
    10021 agggagctgt gggtttcgtg cttaagacca ggtctgccgc tgtgagggac ggagatagaa
    10081 tacttgctac tatcagggcc accgagatcg gacacaatgg aagatctcag ggacttgctg
    10141 ctcctaatgt cagatctcag gcagctgctc atagagccgt gttgcgtaga gcaaggttgg
    10201 acccatctga gattgacttc atcgaagcac atggtactgg aaccacattg ggagatcttt
    10261 gcgaagtgca gggtatcaac gactcttttg tctctcctaa gaaaagagcc aaccctcttg
    10321 tagtctctgc atctaagtca accatcggtc acaccgagcc atctgctggt ttggtcggaa
    10381 tcctttcagc tcttatgtca ttcgagaaga ggatcgtgcc tagattggca tatcttactg
    10441 agtctaatgt gaacccagca ttggacgcat cagtcgttcc attgcacttt cctacaaagc
    10501 acatcgagct tagagctgat gtgccttaca aggccgtggt gatgtcttac ggattcgcag
    10561 gtacccttgc cgacatcgtc cttgagtcag aggttccaca accaactcca gccgtcgctc
    10621 aggacacagc cggtcaacag ccaatgcttt tcgtgttgtc agccaaaacc ccaagggcac
    10681 ttgcagccta catcgagctt taccttggtt tccttaggca cgccgaccct ggacttttcg
    10741 caaggatctg ctacaccgca tgtgtcgcaa gggagcacta caagcacagg gtcgcctgcg
    10801 ttgcaacaga cttggtcgac cttatcgctc agcttgagac taggcttgtg cagactgcct
    10861 atgcaggagg aggaggtgcc agggccgcta ggaccggtcc tcttgtgttt gctttttctg
    10921 gacagggtac tcagttccct gctatggcag caccacttgc taggagatac gcaagattcg
    10981 gagagatcgt gggtggttgt gctaggatgg caagagagct ttcaggtttc ccagtggatg
    11041 gtattcttct tggtgacgat gtgacacctg tgaaggacaa ctctgctgca gctgaggtgc
    11101 actctgaggt ggaccagatc tgcatatttg tgtaccagta tgccatgtgt agatggttgg
    11161 gtgagcttgg tgtggagcca aaggctgcca taggtcactc tttgggagag atcacagcag
    11221 ctgtcatcgc aggagcactt cctttcgaag ccgccttgga tcttgtcgtc actagagcca
    11281 ggttgcttaa gccatgcgcc gagcaacctt ctggaatggc agccttggcc tgcaccccag
    11341 acgtcgcatc aaagttgaca cttggtgctt cagtgtctgt gtcagtctac aacggacctc
    11401 agtctatctg cctttctggt gcttctgccg agcttgacga tgccgtgagg gccgctaagg
    11461 ccaggaacat caaggcaact agacttcagg tcgaccaggg tttccactca ccttgcgtcg
    11521 acgccgcagt gccaggtttg caggcttggt gcgcagctca cagggcatca gctgctccat
    11581 tgaagatgcc tctttactct accgtgagag gggacgtcgt tccaaaggga gccgcccttg
    11641 atccagagca ctgggtggcc cacgctagga accctgtgtt gttcgcccag acagcccagg
    11701 ctcttaaaga gtcacttcca catggaatca ctttggacgt cggtcctcag gccgtggctt
    11761 ggtctttgct tcttttgaac ggtctttcag ctaccagaac agtcgctgcc ggagccaaga
    11821 agggagcaga ccaggagagg gcccttttgg gtgctcttgg agccttgttc gagcagcaca
    11881 aggtcactcc tgactttggt agactttacg ctcctttgga aaagacaagg atcccaacct
    11941 acccatttga gagagctaga tgctacccaa ccttcatacc atcaaggttc gcccacggtg
    12001 ctgctgctgc tgccaagaca gatggtgagg tcctttctgc cgaagaagaa aatgtggcac
    12061 ctgtgggatt gttgacaaag gaggatttga gggcagcttt ggtcgcttgt ttgagggcca
    12121 cattggagct taggcctgac gaggagcttg acgaagcaga gcctttgacc gtgcacggag
    12181 tggactctat cggatttgca aagcttagga agcacgtcga ggacaggtgg ggtcttgaca
    12241 tcccagtcgt gtactggtca gacgccttca ccgtcggaga gatgttgggt aacttggtcg
    12301 gacagtatga cgtggtgtct actgctgcca ggtagttaac tagcttgtcc tgctttaatg
    12361 agatatgcga gaagcctatg atcgcatgat atttgctttc aattctgttg tgcacgttgt
    12421 aaaaaacctg agcatgtgta gctcagatcc ttaccgccgg tttcggttca ttctaatgaa
    12481 tatatcaccc gttactatcg tatttttatg aataatattc tccgttcaat ttactgattg
    12541 taccctacta cttatatgta caatattaaa atgaaaacaa tatattgtgc tgaataggtt
    12601 tatagcgaca tctatgatag agcgccacaa taacaaacaa ttgcgtttta ttattacaaa
    12661 tccaatttta aaaaaagcgg cagaaccggt caaacctaaa agactgatta cataaatctt
    12721 attcaaattt caaaagtgcc ccaggggcta gtatctacga cacaccgagc ggcgaactaa
    12781 taacgctcac tgaagggaac tccggttccc cgccggcgcg catgggtgag attccttgaa
    12841 gttgagtatt ggccgtccgc tctaccgaaa gttacgggca ccattcaacc cggtccagca
    12901 cggcggccgg gtaaccgact tgctgccccg agaattatgc agcatttttt tggtgtatgt
    12961 gggccccaaa tgaagtgcag gtcaaacctt gacagtgacg acaaatcgtt gggcgggtcc
    13021 agggcgaatt ttgcgacaac atgtcgaggc tcagcaggac cgctactaga attcgagctc
    13081 ggagtggcag acatactgtc ccacaaatga agatggaatc tgtaaaagaa aacgcgtgaa
    13141 ataatgcgtc tgacaaaggt taggtcggct gcctttaatc aataccaaag tggtccctac
    13201 cacgatggaa aaactgtgca gtcggtttgg ctttttctga cgaacaaata agattcgtgg
    13261 ccgacaggtg ggggtccacc atgtgaaggc atcttcagac tccaataatg gagcaatgac
    13321 gtaagggctt acgaaataag taagggtagt ttgggaaatg tccactcacc cgtcagtcta
    13381 taaatactta gcccctccct cattgttaag ggagcaaaat ctcagagaga tagtcctaga
    13441 gagagaaaga gagcaagtag cctagaagta gtcaaggcgg cgaagtattc aggcaggtgg
    13501 ccaggaagaa gaaaagccaa gacgacgaaa acaggtaaga gctaagctta atggtccaag
    13561 acacctcaag cgctagtacc agtccaattt tgacaagatg gtatatagat actagacctt
    13621 tgactgcttc taccgcagcc ttgccactgt tggaaactct gcagcctgca gaccagataa
    13681 gtgtccaaaa gtactaccat ctgaaggata agcatatgtc tctcgcatcc aatcttctca
    13741 agtacttgtt tgttcacagg aattgccgaa tcccatggtc tagcattgta atatcaagaa
    13801 ctccagatcc ccatagaagg ccctgttata taccaccctc tggttctcaa gaggacagct
    13861 ttaaggatgg ctacactgga attaatgtcg agttcaacgt atcccatcaa gcaagcatgg
    13921 tggcaatagc aggcactgca tttacaccca actctggagg tgattccaaa cttaagccag
    13981 aggttggcat agacattact tgcgtgaatg agcgacaggg gcgaaacggt gaggagcgat
    14041 cccttgaaag tttgaggcag tacatagata ttttctcaga ggttttttcc accgcagaga
    14101 tggcaaacat cagaagattg gatggggtgt catcaagctc cttgagcgcc gataggttgg
    14161 tggactatgg ttaccgattg ttttacactt attgggctct taaggaagca tacatcaaga
    14221 tgacagggga agccctgctg gcaccatggc tcagggagtt ggaatttagt aatgtcgttg
    14281 ccccagcagc agtggccgaa tccggagatt ccgccggaga ctttggagag ccttataccg
    14341 gggtacgaac aaccctttac aaaaacttgg tcgaggatgt taggatagag gtggctgctc
    14401 tgggaggtga ctacttgttc gccactgctg cacgaggggg cggtataggt gcctccagcc
    14461 gaccaggggg aggccctgat ggctcaggaa ttaggagtca agatccctgg cgaccattta
    14521 aaaagctcga catagagaga gacatacagc cttgcgccac tggagtctgt aattgtctgt
    14581 ctcgaggtag ttaactagct taccgcactg tgtgtggttt ctcaagacca agacagctaa
    14641 agcctaaagt cagagatcta atatgtgtat tgttattcat gacaccacag ctgccacttt
    14701 tggtgttatg atctgtttgt agaagtagga attctttttt ttctacttaa taatagctta
    14761 aagagctgtg caatttggtc tgtatttttt gtgtattttg cactcattat ttgtgaacag
    14821 tttgagaact atttattttc taagatttgt gcacgtatga accacttttc atctatatac
    14881 caccatgttt attctgcatc tatgggattg agtttgaata ttcgttgatc aacaaagtta
    14941 tatttggtgg atactacttg aaggtgcata tactttgtgc tcatatattt agttgatatt
    15001 ctggattttg agctggacaa attgatcaag gtagtctaat ctggtctggt tactaataaa
    15061 actcaagaga tcactgtctc gctttacgaa ttcccatggg gaggaattcc aatcccacaa
    15121 aaatctgagc ttaacagcac agttgctcct ctcagagcag aatcgggtat tcaacaccct
    15181 catatcaact actacgttgt gtataacggt ccacatgccg gtatatacga tgactggggt
    15241 tgtacaaagg cggcaacaaa cggcgttccc ggagttgcac acaagaaatt tgccactatt
    15301 acagaggcaa gagcagcagc tgacgcgtac acaacaagtc agcaaacaga caggttgaac
    15361 ttcatcccca aaggagaagc tcaactcaag cccaagagct ttgctaaggc cctaacaagc
    15421 ccaccaaagc aaaaagccca ctggctcacg ctaggaacca aaaggcccag cagtgatcca
    15481 gccccaaaag agatctcctt tgccccggag attacaatgg acgatttcct ctatctttac
    15541 gatctaggaa ggaagttcga aggtgaaggt gacgacacta tgttcaccac tgataatgag
    15601 aaggttagcc tcttcaattt cagaaagaat gctgacccac agatggttag agaggcctac
    15661 gcagcaagtc tcatcaagac gatctacccg agtaacaatc tccaggagat caaatacctt
    15721 cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta attgcatcaa gaacacagag
    15781 aaagacatat ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt
    15841 cataaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc tactgaatct
    15901 aaggccatgc atggagtcta agattcaaat cgaggatcta acagaactcg ccgtcaagac
    15961 tggcgaacag ttcatacaga gtcttttacg actcaatgac aagaagaaaa tcttcgtcaa
    16021 catggtggag cacgacactc tggtctactc caaaaatgtc aaagatacag tctcagaaga
    16081 tcaaagggct attgagactt ttcaacaaag gataatttcg ggaaacctcc tcggattcca
    16141 ttgcccagct atctgtcact tcatcgaaag gacagtagaa aaggaaggtg gctcctacaa
    16201 atgccatcat tgcgataaag gaaaggctat cattcaagat ctctctgccg acagtggtcc
    16261 caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagaggttc caaccacgtc
    16321 tacaaagcaa gtggattgat gtgacatctc cactgacgta agggatgacg cacaatccca
    16381 ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg agaggacacg
    16441 ctcgagtata agagctcatt tttacaacaa ttaccaacaa caacaaacaa caaacaacat
    16501 tacaattaca tttacaatta tcgatacaat gaggtctact atctctttgt ctagtctcct
    16561 tgagagactg agcaaattgt catcccgatc catcgcaatt acctgcggcg tagtcttggc
    16621 aagtgcaata gcattcccaa ttatccgaag agactatcaa acgttccttg aagtcggtcc
    16681 gagctatgcc ccacagaact tccgaggcta tatcactgtt tgtgtcttgt cactttttag
    16741 gcaagagcag aaagggttgg caatctatga caggcttcca gaaaagaggc gttggcttgc
    16801 cgacttgccg tttcgtgaag ggccaaggcc atccataacc tcccacatta tacagcgtca
    16861 gcgtactcag ctggtagatc aagagtttgc aaccagagaa cttatcgata aggtgatccc
    16921 acgtgtgcaa gcaagacaca cagacaaaac ttttctcagt acgtcaaaat ttgagtttca
    16981 tgcaaaggcc atcttccttc tcccctctat ccctattaat gatcctctta acattccctc
    17041 acacgatacg gtaagaagaa ccaagcgtga gatcgctcac atgcatgatt accatgattg
    17101 ctctctgcac ttggctcttg ctcctcagga tggtaaggaa gttttgaaga aggggtgggg
    17161 ccagcgtcac ccactggccg gaccaggagt cccaggccct cctactgagt ggaccttcct
    17221 ttacgcacca aggaacgaag aggaggccag agttgtcgag atgatagtcg aagctagtat
    17281 tggctacatg actaacgatc ctgctggtaa gattgtcgaa aatgccaaag gtagttaact
    17341 agcttgctct caagatcaaa ggcttaaaaa gctggggttt tatgaatggg atcaaagttt
    17401 ctttttttct tttatatttg cttctccatt tgtttgtttc atttcccttt ttgttttcgt
    17461 ttctatgatg cacttgtgtg tgacaaactc tctgggtttt tacttacgtc tgcgtttcaa
    17521 aaaaaaaacc gctttcgttt tgcgttttag tcccattgtt ttgtagctct gagtgatcga
    17581 attgatgcct ctttattcct tttgttccct ataatttctt tcaaaactca gaagaaaaac
    17641 cttgaaactc tttgcaatgt taatataagt attgtataag atttttattg atttggttat
    17701 tagtcttact tttgctacct ccatcttcac ttggaactga tattctgaat agttaaagcg
    17761 ttacatgtgt tccattcaca aatgaactta aactagcaca aagtcagata ttttaagatc
    17821 gcaccatttc gctcagagaa ttcgcatgcg gaggtcaaca tggtggagca cgacactctg
    17881 gtctactcca aaaatgtcaa agatacagtc tcagaagatc aaagggctat tgagactttt
    17941 caacaaagga taatttcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc
    18001 atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga
    18061 aaggctatca ttcaagatct ctctgccgac agtggtccca aagatggacc cccacccacg
    18121 aggagcatcg tggaaaaaga agaggttcca accacgtcta caaagcaagt ggattgatgt
    18181 gacatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc
    18241 tctatataag gaagttcatt tcatttggag aggacacgct cgagtataag agctcatttt
    18301 tacaacaatt accaacaaca acaaacaaca aacaacatta caattacatt tacaattatc
    18361 gatacaatgg cacctatttc atccacgtgg agcaggttga taaggttcgt ggctgtagaa
    18421 acttccctgg ttcatattgg tgagccaatt gatgcaacga tggacgttgg cctcgcccgt
    18481 agagaaggta agacaattca agcatacgaa ataatcggaa gcggcagtgc cctcgatctt
    18541 tctgcacaag tctctaagaa cgtactcaca gttagggaat tgttgatgcc gctcagtcga
    18601 gaagagatta agaccgttcg atgcctcggc ttgaactatc cggtccacgc cacagaagct
    18661 aacgtggccg tgccgaagtt tcctaacctg ttctataagc ccgtaacatc attgattggt
    18721 ccaggcggtc ttattactat tccctccgtc gttcaacccc ccaaggagca ccagtcagat
    18781 tacgaggctg agctggttat agtaataggt aaggcagcaa agaacgtatc agaggatgag
    18841 gctttggatt atgtattggg ctataccgct gcaaacgata tatctttccg taagcatcaa
    18901 ttggctgtca gtcaatggag cttcagcaag ggctttgatg gaactaatcc acttggtccc
    18961 tgcttggttt ccgcctccag tatccctgac ccacaggata ttccaataca gtgcaaattg
    19021 aatggtggag tcgtccaaaa tggcaacacc agagatcaaa tcttcaacgt taagaaaacc
    19081 atatcatttc tttcccaggg gactactttg gagcccggtt ccattatcct taccgggacc
    19141 ccagatgggg tggggttcgt acgaaacccg cccctgtatt tgaaggatgg cgatgaggtt
    19201 atgacttgga tcggtagtgg aatcggaact ctcgcaaata cagttcgaga agaacaaacg
    19261 tgctttgcat caggcggcca cgaatagctt gtcctgcttt aatgagatat gcgagaagcc
    19321 tatgatcgca tgatatttgc tttcaattct gttgtgcacg ttgtaaaaaa cctgagcatg
    19381 tgtagctcag atccttaccg ccggtttcgg ttcattctaa tgaatatatc acccgttact
    19441 atcgtatttt tatgaataat attctccgtt caatttactg attgtaccct actacttata
    19501 tgtacaatat taaaatgaaa acaatatatt gtgctgaata ggtttatagc gacatctatg
    19561 atagagcgcc acaataacaa acaattgcgt tttattatta caaatccaat tttaaaaaaa
    19621 gcggcagaac cggtcaaacc taaaagactg attacataaa tcttattcaa atttcaaaag
    19681 tgccccaggg gctagtatct acgacacacc gagcggcgaa ctaataacgc tcactgaagg
    19741 gaactccggt tccccgccgg cgcgcatggg tgagattcct tgaagttgag tattggccgt
    19801 ccgctctacc gaaagttacg ggcaccattc aacccggtcc agcacggcgg ccgggtaacc
    19861 gacttgctgc cccgagaatt atgcagcatt tttttggtgt atgtgggccc caaatgaagt
    19921 gcaggtcaaa ccttgacagt gacgacaaat cgttgggcgg gtccagggcg aattttgcga
    19981 caacatgtcg aggctcagca ggaccgcttg tggaattcct cgagggagtc atcaaaatat
    20041 ttagcagcat tccagattgg gttcaatcaa caaggtacga gccatatcac tttattcaaa
    20101 ttggtatcgc caaaaccaag aaggaactcc catcctcaaa ggtttgtaag gaagaattct
    20161 cagtccaaag cctcaacaag gtcagggtac agagtctcca aaccattagc caaaagctac
    20221 aggagatcaa tgaagaatct tcaatcaaag taaactactg ttccagcaca tgcatcatgg
    20281 tcagtaagtt tcagaaaaag acatccaccg aggacttaaa gttagtgggc atctttgaaa
    20341 gtaatcttgt caacatcgag cagctggctt gtggggacca gacaaaaaag gaatggtgca
    20401 gaattgttag gcgcacctac caaaagcatc tttgccttta ttgcaaagat aaagcagatt
    20461 cctctagtac aagtggggaa caaaataacg tggaaaagag ctgtcctgac agcccactca
    20521 ctaatgcgta tgacgaacgc agtgacgacc acaaaagaat tccctctata taagaaggca
    20581 ttcattccca tttgaaggat catcagatac tgaaccaata tttctcaatg gcctcttttg
    20641 aaaattcact ctctgtgttg atcgtgggag ccggtttggg tggtctagca gctgctatcg
    20701 ctctccgacg acagggccac gtggttaaga tctacgaatc atctagcttt aaggccgagc
    20761 tcggcgcagg cttggcagtt ccaccgaata cactgcgtag tctacagcaa ttgggttgta
    20821 acactgaaaa ccttaacggc gttgataatc tctgttttac tgccatggga tatgatgggt
    20881 cagtgggcat gatgaacaat atgactgatt accgtgaagc atatggcact agttggatca
    20941 tggttcatcg tgtcgatttg cataacgagt tgatgagagt tgctttggac ccagggggat
    21001 tgggccctcc cgcaacgctt cacctgaatc atcgagtcac gttttgtgat gtagatgctt
    21061 gtacggtgac atttaccaac ggtacaactc aaagtgcaga tcttatcgtt ggggctgacg
    21121 ggatacgttc aactatccgt agatttgtct tggaggagga cgtgaccatt cccgcaagcg
    21181 gaattgtggg gtttcgatgg ttggtacagg ctgatgccct cgatccatat cctgaactcg
    21241 attggatagt caaaaagcca ccgctcggag ctaggttgat ttctacgccc cagaatccac
    21301 aatctggagt gggactggca gatcgtagaa ctattattat atacgcttgt cgtgggggga
    21361 caatggtaaa tgtgctggcc gtacacgatg acgagcgaga tcagaatact gcagattgga
    21421 gcgttccagc atcaaaggat gacttgtttc gagtattcca tgactaccat ccgagattca
    21481 gacggctctt ggaattggct caagatataa atctctggca gatgagggtt gtgccggtgc
    21541 tcaagaaatg ggtgaacaaa cgagtgtgcc tgcttggaga tgccgctcat gccatgcttc
    21601 cgactctcgg ccagggtttc gggatgggat tggaggacgc cgtagccctt ggtactctct
    21661 tgccaaaagg tacgactgct agtcaaatag agacaagact ggctgtctac gaacagctta
    21721 gaaaggaccg agcagagttt gtagccgctg aaagttatga ggagcagtat gtccccgaga
    21781 agaggggctt gtacttgcgt agcaaggaac ttagagatag agtcatgggg tatgacataa
    21841 aagtggagtc tgagaaggta ctggaaacct tgcttaggag ctctaactca gcaggtagtt
    21901 aactagcttg tcaagcagat cgttcaaaca tttggcaata aagtttctta agattgaatc
    21961 ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa
    22021 taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc
    22081 aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat
    22141 cgcgcgcggt gtcatctatg ttactagatc gacgctgagc ttgtcttcga ggatgcacat
    22201 gtgaccgagg gacacgaagt ggttt
//
