LOCUS       sequence-45075-e         268 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Dillin A, Crawford DK, Kenyon C
  TITLE     Timing requirements for insulin/IGF-1 signaling in C. elegans.
  JOURNAL   Science. 2002 Oct 25;298(5594):830-4.
  PUBMED    12399591
REFERENCE   2  (bases 1 to 268)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2002-10-25"; volume: "298(5594)"; pages: "830-4"
COMMENT     Sequence Label: pAD12
FEATURES             Location/Qualifiers
     source          1..268
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(60..79)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(61..79)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(101..121)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(101..119)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(140..156)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(140..156)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(153..175)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    164..180
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(188..194,195..212,213..218))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    233..254
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gtatcgataa gcttgatatn cnnnnnnann ngagcccggg ggatccacta gtgcttcgcc
       61 cctatagtga gtcgtattac gcgagctcca gcttttgttc cctttagtga gggttaattg
      121 cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
      181 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
      241 gctaactcac attaattgcg ttgcgctc
//
