LOCUS       sequence-450762-       10078 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMV-Intron-GagPol (FMLV). Expresses FMLV (Friend Murine Leukemia 
            Virus) Gag-Pol for VLP production..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10078)
  AUTHORS   Ramadoss GN, Namaganda SJ, Kumar MM, Hamilton JR, Sharma R, Chow KG,
            Workley LA, Macklin BL, Sun M, Ha AS, Liu JC, Fellmann C, Watry HL, 
            Dierks PH, Bose RS, Jin J, Perez BS, Sandoval Espinoza CR, Matia MP,
            Lu SH, Judge LM, Shy BR, Nussenzweig A, Adamson B, Murthy N, Doudna 
            JA, Kampmann M, Conklin BR
  TITLE     Characterizing and controlling CRISPR repair outcomes in nondividing
            human cells.
  JOURNAL   Nat Commun. 2025 Nov 17;16(1):9883. doi: 10.1038/s41467-025-66058-3.
  PUBMED    41249169
REFERENCE   2  (bases 1 to 10078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-11-17"; volume: "16(1):9883. doi"; pages: " 
            10.1038/s41467-025-66058-3"
COMMENT     Sequence Label: pCF153_Gag-Pol
FEATURES             Location/Qualifiers
     source          1..10078
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        97..476
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        477..680
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     631..651
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     677..701
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          792..1364
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(6783..6802)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6848..6903
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6902..6921)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7261..7277)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7261..7277)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7274..7296)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7285..7301
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7309..7315,7316..7333,7334..7339))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7354..7375
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7492..7509)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7663..8251)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7743..7762)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8422..9213,9214..9282))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9045..9064
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9283..9387)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9413..9868)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9550..9571)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9762..9781
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9995..10017
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10009..10026
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10010..10026
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10036..10055
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10036..10054
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcggccgctc tagagagctt ggcccattgc atacgttgta tccatatcat aatatgtaca
       61 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
      121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      481 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      541 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      601 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      661 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac
      721 gctgttttga cctccataga agacaccggg accgatccag cctccggtcg accgatcctg
      781 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat
      841 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct
      901 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac
      961 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc
     1021 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct
     1081 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga
     1141 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc
     1201 gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt
     1261 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg
     1321 gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc
     1381 tggttgttgt gctgtctcat cattttggca aagctcgagc tgtatttgtc tgacaacatg
     1441 ggccaggctg ttaccacccc cttaagtttg actttagacc actggaagga tgtcgaacgg
     1501 acagcccaca acctgtcggt agaggttaga aaaaggcgct gggttacatt ctgctctgca
     1561 gaatggccaa ccttcaacgt cggatggcca cgagacggca cttttaaccc agacattatt
     1621 acacaggtta agatcaaggt cttctcacct ggcccacatg gacatccgga tcaggtcccc
     1681 tacatcgtga cctgggaagc tatagcagta gacccccctc cctgggtcag acccttcgtg
     1741 caccctaaac ctcccctctc tcttccccct tcagccccct ctctcccacc tgaaccccca
     1801 ctctcgaccc cgccccagtc ctccctctat ccggctctca cttctccttt aaacaccaaa
     1861 cctaggcctc aagtccttcc tgatagcgga ggaccactca ttgatctact cacggaggac
     1921 cctccgcctt accgggaccc agggccaccc tctcctgacg ggaacggcga tagcggagaa
     1981 gtggccccta cagaaggagc ccctgaccct tccccaatgg tatcccgcct gcggggaaga
     2041 aaagaacccc ccgtggcgga ttctactacc tctcaggcgt tcccccttcg cctgggaggg
     2101 aatggacagt atcaatactg gccattttcc tcctctgacc tctataactg gaaaaataac
     2161 aacccctctt tctccgagga cccagctaaa ttgacagctt tgatcgagtc cgttctcctt
     2221 actcatcagc ccacttggga tgactgccaa cagctattag ggaccctgct gacgggagaa
     2281 gaaaaacagc gagtgctcct agaggcccga aaggcggttc gaggggagga cggacgccca
     2341 actcagctgc ccaatgacat taatgatgct tttcccttgg aacgtcccga ctgggactac
     2401 aacacccaac gaggtaggaa ccacctagtc cactatcgcc agttgctcct agcgggtctc
     2461 caaaacgcgg gcagaagccc caccaatttg gccaaggtaa aagggataac ccagggacct
     2521 aatgagtctc cctcagcctt tttagagaga ctcaaggagg cctatcgcag atacactcct
     2581 tatgaccctg aggacccagg gcaagaaacc aatgtggcca tgtcattcat ctggcagtcc
     2641 gccccggata tcgggcgaaa gttagagcgg ttagaagatt tgaagagtaa gaccttagga
     2701 gacttagtga gggaagctga aaagatcttt aataaacgag aaaccccgga agaaagagag
     2761 gaacgtatta ggagagaaac agaggaaaag gaagaacgcc gtagggcaga ggatgtgcag
     2821 agagagaagg agagggaccg cagaagacat agagaaatga gtaagttgct ggctactgtc
     2881 gttagcgggc agagacagga tagacaggga ggagagcgaa ggaggcccca actcgaccac
     2941 gaccagtgtg cctactgcaa agaaaaggga cattgggcta gagattgccc caagaagcca
     3001 agaggacccc ggggaccacg accccaggcc tccctcctga ccttagacga ttagggaggt
     3061 cagggtcagg agcccccccc tgaacccagg ataaccctca gagtcggggg gcaacccgtc
     3121 accttcctag tggatactgg ggcccaacac tccgtgctga cccaaaatcc tggaccccta
     3181 agtgacaagt ctgcctgggt ccaaggggct actggaggga agcggtatcg ctggaccacg
     3241 gatcgccgag tgcacctagc caccggtaag gtcacccatt ctttcctcca tgtaccagat
     3301 tgcccctatc ctctgctagg aagagatttg ctgactaaac taaaagccca aattcacttt
     3361 gagggatcag gagctcaggt tgtgggacca atgggacagc ccctgcaagt gctgacccta
     3421 aacatagaag atgagtatcg gctacatgag acctcaaaag ggccagatgt gcctctaggg
     3481 tccacatggc tctctgattt tccccaggcc tgggcagaaa ccgggggcat ggggctggcc
     3541 gttcgccaag ctcctctgat catacctctg aaggcaacct ctacccccgt gtccataaaa
     3601 caatacccca tgtcacaaga agccagactg gggatcaagc cccacataca gagactgctg
     3661 gatcagggaa ttctggtacc ctgccagtcc ccctggaaca cgcccctgct acccgttaag
     3721 aaaccgggga ctaatgatta taggcctgtc caggatctga gagaagtcaa caagcgggtg
     3781 gaagacatcc accccaccgt gcccaaccct tacaacctct tgagcgggct cccaccgtcc
     3841 caccagtggt acactgtgct tgacttaaaa gatgcttttt tctgcctgag actccacccc
     3901 accagtcagt ctctcttcgc ctttgagtgg agagatccag agatgggaat ctcaggacaa
     3961 ttaacctgga ccagactccc gcagggtttc aaaaacagtc ccaccctgtt tgatgaagcc
     4021 ctgcacaggg acctcgcaga cttccggatc cagcacccag acctgattct gctccagtat
     4081 gtagatgact tactgctggc cgccacttct gagcttgact gtcaacaagg tacgcgggcc
     4141 ctgttacaaa ccctagggga cctcggatat cgggcctcgg ccaagaaagc ccaaatttgc
     4201 cagaaacagg tcaagtatct ggggtatctt ctaaaagagg gtcagagatg gctgactgag
     4261 gccagaaaag agactgtgat ggggcagcct actccgaaga cccctcgaca actaagggag
     4321 ttcctaggga cggcaggctt ctgtcgcctc tggatccctg ggtttgcaga aatggcagcc
     4381 cccttgtacc ctctcaccaa aacggggact ctgtttgagt ggggcccaga ccagcaaaag
     4441 gcctaccaag agatcaagca ggctctctta actgcccctg ccctgggatt gccagacttg
     4501 actaagccct tcgaactttt tgttgacgag aagcagggct acgccaaagg tgtcctaacg
     4561 caaaaactgg ggccttggcg tcggccggtg gcctacctgt ccaaaaagct agacccagtg
     4621 gcagctgggt ggcccccttg cctacggatg gtagcagcca tcgccgttct gaccaaagac
     4681 gctggcaagc tcaccatggg acagccacta gtcattctgg ccccccatgc agtagaggca
     4741 ctagttaagc aaccccctga tcgctggctc tccaacgccc gaatgaccca ctaccaggct
     4801 ctgcttctgg acacggaccg agtccagttc ggaccaatag tggccctaaa cccagctacg
     4861 ctgctccctc tacctgagga ggggctgcaa catgactgcc ttgacatctt ggctgaagcc
     4921 cacggaacta gaccagatct tacggaccag cctctcccag acgctgacca cacctggtac
     4981 acagatggga gcagcttcct gcaagagggg cagcgcaagg ccggagcagc agtaaccacc
     5041 gagaccgagg tagtctgggc caaagcactg ccagccggga catcggccca aagagctgag
     5101 ttgatagcgc tcacccaagc cttaaaaatg gcagaaggta agaagctgaa tgtttacacc
     5161 gatagccgtt atgcttttgc cactgcccat attcacggag aaatatatag aaggcgcggg
     5221 ttgctcacat cagaaggaaa agaaatcaaa aataaggacg agatcttggc cctactgaag
     5281 gctctcttcc tgcccaaaag acttagcata attcattgcc cgggacatca gaagggaaac
     5341 cgcgcggagg caaggggcaa caggatggcc gaccaagcgg cccgagaagt agccactaga
     5401 gaaactccag agacttccac acttctgata gaaaattcag ccccctatac tcatgaacat
     5461 tttcactata cggtgactga cataaaagat ctgactaaac taggggccac ttatgacgat
     5521 gcaaagaagt gttgggttta tcagggaaag cctgtaatgc ctgatcaatt cacctttgaa
     5581 ctattagatt ttcttcatca attgacccac ctcagtttct caaaaacaaa ggctcttcta
     5641 gaaaggaact actgtcctta ttacatgctg aaccgggatc gaacgctcaa agacatcact
     5701 gagacttgcc aagcctgtgc acaggtcaat gccagcaagt ctgccgtcaa acaagggact
     5761 agagttcgag ggcaccgacc cggcacccac tgggaaattg atttcactga ggtaaaacct
     5821 ggcctgtatg ggtataaata tcttttagtt ttcatagaca ctttctctgg atgggtagaa
     5881 gctttcccaa ccaagaaaga aactgccaaa gttgtaacca agaagctact agaagaaatc
     5941 ttccccagat tcggcatgcc acaggtattg ggaaccgaca atgggcctgc cttcgtctcc
     6001 aaggtaagtc agacagtagc cgatttactg ggggttgatt ggaaactaca ttgtgcttac
     6061 agaccccaga gttcaggtca ggtagaaaga atgaatagga caatcaagga gactttaact
     6121 aaattgacgc ttgcaactgg ctctagggac tgggtgctcc tgcttcccct agccctgtat
     6181 cgagcccgca acacgccggg cccccatggt ctcaccccat atgaaatctt atatggggca
     6241 cccccgcccc ttgtaaactt ccctgatcct gacatggcaa aggttactca taacccctct
     6301 ctccaagccc atttacaggc actctacctg gtccagcacg aagtctggag accgttggcg
     6361 gcagcttacc aagaacaact ggaccggccg gtagtgcctc accctttccg agtcggtgac
     6421 acagtgtggg tccgcagaca ccaaactaaa aatctagaac cccgctggaa aggaccttat
     6481 accgtcctac tgactacccc caccgctctc aaagtggacg gcattgcagc gtggatccac
     6541 gctgcccacg taaaggctgc cgacaccagg attgagccac catcggaatc gacatggcgt
     6601 gttcaacgct ctcaaaatcc cctaaagata agattgaccc gcgggacctc ctaatcccct
     6661 taattctctt cctgtcgacc tgcagatccg gccctcgagg aattcactcc tcaggtgcag
     6721 gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa ataccactga
     6781 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact
     6841 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc
     6901 tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt
     6961 tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga
     7021 ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg
     7081 acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta
     7141 aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc
     7201 atagctgtcc ctcttctctt atggagatcc ctcgacggat cggccgcaat tcgtaatcat
     7261 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     7321 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     7381 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     7441 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     7501 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     7561 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     7621 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     7681 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     7741 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     7801 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     7861 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     7921 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     7981 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     8041 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     8101 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     8161 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     8221 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     8281 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     8341 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     8401 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     8461 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     8521 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     8581 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     8641 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     8701 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     8761 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     8821 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     8881 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     8941 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     9001 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     9061 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     9121 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     9181 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     9241 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     9301 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     9361 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta
     9421 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     9481 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     9541 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     9601 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     9661 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     9721 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     9781 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     9841 gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg
     9901 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     9961 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
    10021 gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca ccgcggtg
//
