LOCUS       sequence-450765-        9985 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full length mouse PROSER1 with 2X C-terminal FLAG tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9985)
  AUTHORS   Fleming A, Knatko EV, Li X, Zoch A, Heckhausen Z, Stransky S, Brenes
            AJ, Sidoli S, Hajkova P, O'Carroll D, Rasmussen KD
  TITLE     PROSER1 modulates DNA demethylation through dual mechanisms to 
            prevent syndromic developmental malformations.
  JOURNAL   Genes Dev. 2024 Nov 19. doi: 10.1101/gad.352176.124.
  PUBMED    39562138
REFERENCE   2  (bases 1 to 9985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2024 Nov 19. doi: 10.1101/gad.352176.124."
COMMENT     Sequence Label: PiggyBac-mProser1-FLAG-IRES-Blast
FEATURES             Location/Qualifiers
     source          1..9985
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(107..126)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      920..1508
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1409..1428
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1662..1679
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1796..1817
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1832..1837,1838..1855,1856..1862)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1870..1886
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1894..1910
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1931..1949
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   1973..2035
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    complement(2206..2239)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    2312..2333
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2348..2353,2354..2371,2372..2378)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2386..2402
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2410..2426
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2769..2788
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    2787..2842
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     2888..2907
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     misc_feature    join(3394..3402,3403..3405,3406..3980)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3567..3586)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     3796..3813
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     protein_bind    4132..4156
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(4184..4231)
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     protein_bind    complement(7006..7030)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(7067..7086)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          7094..8110
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(7148..7170)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(8111..8387)
                     /label=chicken beta-actin promoter
     enhancer        8389..8768
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     repeat_region   9052..9086
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(9104..9123)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9105..9123)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9130..9147)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9130..9146)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9139..9161)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9288..9743
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9375..9394)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9585..9606
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9769..9873
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9874..9942,9943..9985,1..749)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
       61 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      121 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      181 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      241 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      301 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      361 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      421 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      481 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      541 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      601 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      661 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      721 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
      781 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
      841 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
      901 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      961 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1021 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1081 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1141 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1201 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1261 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1321 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1381 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1441 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1501 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1561 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1621 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1681 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1741 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     1801 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     1861 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     1921 ccaagctcga aattaaccct cactaaaggg aacaaaagct ggagctcttt aaccctagaa
     1981 agataatcat attgtgacgt acgttaaaga taatcatgcg taaaattgac gcatgtgttt
     2041 tatcggtctg tatatcgagg tttatttatt aatttgaata gatattaagt tttattatat
     2101 ttacacttac atactaataa taaattcaac aaacaattta tttatgttta tttatttatt
     2161 aaaaaaaaac aaaaactcaa aatttcttct ataaagtaac aaaacataac ttcgtatagc
     2221 atacattata cgaagttatc tcgacgcggc cgcggatccg ctggcacgac aggtttcccg
     2281 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     2341 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     2401 aatttcacac aggaaacagc tatgaccatg attacgccaa gcttgggctg caggtcgagg
     2461 gatctccata agagaagagg gacagctatg actgggagta gtcaggagag gaggaaaaat
     2521 ctggctagta aaacatgtaa ggaaaatttt agggatgtta aagaaaaaaa taacacaaaa
     2581 caaaatataa aaaaaatcta acctcaagtc aaggcttttc tatggaataa ggaatggaca
     2641 gcagggggct gtttcatata ctgatgacct ctttatagcc aacctttgtt catggcagcc
     2701 agcatatggg catatgttgc caaactctaa accaaatact cattctgatg ttttaaatga
     2761 tttgccctcc catatgtcct tccgagtgag agacacaaaa aattccaaca cactattgca
     2821 atgaaaataa atttccttta ttagccagaa gtcagatgct caaggggctt catgatgtcc
     2881 ccataatttt tggcagaggg aaaaagatcg acccccctcg gacccgtcgg gccgcgtcgg
     2941 acaaagcaag taaaacctct acagatgtga tatggctgat tatgatcatt acttatctag
     3001 ttaaccctcc cacacataac cagagggcag caattcacga atcccaactg ccgtcggctg
     3061 tccatcactg tccttcacta tggctttgat cccaggatgc agatcgagaa gcacctgtcg
     3121 gcaccgtccg caggggctca agatgcccct gttctcattt ccgatcgcga cgatacaagt
     3181 caggttgcca gctgccgcag cagcagcagt gcccagcacc acgagttctg cacaaggtcc
     3241 cccagtaaaa tgatatacat tgacaccagt gaagatgcgg ccgtcgctag agagagctgc
     3301 gctggcgacg ctgtagtctt cagagatggg gatgctgttg attgtagccg ttgctctttc
     3361 aatgagggtg gattcttctt gagacaaagg catggttgtg gccatattat catcgtgttt
     3421 ttcaaaggaa aaccacgtcc ccgtggttcg gggggcctag acgttttttt aacctcgact
     3481 aaacacatgt aaagcatgtg caccgaggcc ccagatcaga tcccatacaa tggggtacct
     3541 tctgggcatc cttcagcccc ttgttgaata cgcttgagga gagccatttg actctttcca
     3601 caactatcca actcacaacg tggcactggg gttgtgccgc ctttgcaggt gtatcttata
     3661 cacgtggctt ttggccgcag aggcacctgt cgccaggtgg ggggttccgc tgcctgcaaa
     3721 gggtcgctac agacgttgtt tgtcttcaag aagcttccag aggaactgct tccttcacga
     3781 cattcaacag accttgcatt cctttggcga gaggggaaag acccctagga atgctcgtca
     3841 agaagacagg gccaggtttc cgggccctca cattgccaaa agacggcaat atggtggaaa
     3901 ataacatata gacaaacgca caccggcctt attccaagcg gcttcggcca gtaacgttag
     3961 ggggggggga gggagagggg gggggggcgg aattcgatat caagcttatc gataccgtcg
     4021 atctcagtgg tatttgtgag ccagttggcc acaccagcca ccaccttctg ataggcagcc
     4081 tgcacctgag gagtgaattc gatatcaagc ttatcgagcg gcctcgaatc aaccactttg
     4141 tacaagaaag ctgggtcggc gcgcccaccc ttcaaccggt acgcttgtca tcgtcgtcct
     4201 tgtagtcctt gtcatcgtcg tccttgtagt cagatccgag tccggattgc cacccgcttt
     4261 gggataggcc tgtttgcaga gaaaatggtg ttcctggcgt ggagggatag ctaggaaagc
     4321 cgtctgcctg ggccgggtag ctctccagtg ccgatgctga gtggacctgc tgtaacaaag
     4381 ccgactgtgc agctgcactg tgctgtaact cttgcaggga cgactgtgga ggagcctggg
     4441 aaaacccagg gatacctggg agggacagaa ggcctggaaa aacagagcta cccgcagctt
     4501 ggagtcctga tgacaaaccg gcttgtgcca ccagggcgga gttgaagttg gaactgaagg
     4561 ctgaggcaaa tcccgggagg acaggtgcag gcgatggagc cgaggcagcc actggcaacg
     4621 gggtaactgc cacggtagag ggcgcctgca gccctgggaa cgagggcagc gtgggggcac
     4681 cgcttggggc actggagaca gggaagccgg ggtaggacgg gttagaggtg ggtagcgggc
     4741 cctgggtgac agcaaagagc gagggtacag cggtggacag gttgagtggg aaggccgcag
     4801 gcgaggcatt ggaggtgggt agcactgagc gcactgttgc agagctaggg tgtacagctg
     4861 cagatgtggt ggtggtcgac gaccccgcga gggatacagc aggattgagg gagggactgc
     4921 ccggtagagg gaaactatta gtcaatgaag tgaaaggggg aagggcagtg aagacaggcg
     4981 cgatgggcgt ggaggatgcg tgcggcggaa gggacataga accagctaac ggggtgctca
     5041 ggctggagag cccagacagt gcagggttaa ggcaagtaga cacactgatg ggcactgagg
     5101 tcgtggggta cccacggccc agggcacccg acaggcccaa ggtaccacga acaggagtcc
     5161 cggggtgagc tgggcctttg aaggccgagg gtgggggact tgtgggctca ctttttatca
     5221 taacgggcat agttgctgca gcaggggcag atgagatgcc acctgcatgg ggaccgtgca
     5281 aaagtgagcc tgaggagcca cagctgactg gcactgccgt ggagggggta gctaacggag
     5341 actgtgcagg cagcgttggg gctacagacg tggcactagc cacaggagac tgcaggcctg
     5401 gaaacatggc cagccctggg gtggaagacc tctgtgcagc aggaacagcc gcagggggat
     5461 agcacttgtt agtggccgag gcaggagaat ctgagtttcg attgggaaga gaggatagtg
     5521 aagccagtcc acttgtgact gcagatggca aggcggagga attgattaag tgtgtgtcat
     5581 ttgatgtaag aaagcctttt aaggcagaca gaagggggct atttacacca tgtggacccg
     5641 cagcactggc aattacagaa ggagtcgggg tggctatgct gtgggatgct ggtgggaagg
     5701 gcaaaggcag acctgcgaag accgacgaga ggggagctgt gattgggtta ctggctgacc
     5761 cagcagtaga gctggctgag gagtggccgg agaatggggc acacgtggaa gcaaatgtct
     5821 cactggaagc cagagtgacc cttgatatcg agctggcctg agtgggtagg gtgggggtgg
     5881 cagatagacc gggcagtggc actaggccag aaaaaacgga agggacagga gtgggtgtgg
     5941 tgctgtgggt ggaagtaaca ggcgttgcgg gcacggaggg agtccggatc acagtcgggt
     6001 ttggcgtgtt tggctgaggc gttgggaaca ccgccagggc agtgttggtc gcactcattc
     6061 cagccccggt ggcagctgct gacacggatg gatggtttac tgccttggct ggagacacag
     6121 caggtactgg agtcgcagca ggtgtcgaag gattagaaga accatgagga gagaagagct
     6181 gactcgcagg ggtggaaaat gtttgactct gtgtaggttt tgactgactg gaaaggtctt
     6241 cattgtctgt tcctgctgga tttggagtgt acggaggtgg aggagggacg cctggagcga
     6301 tcacattggc taatggtacc agacccgcat tgttgtaagc acttggtgcg atagttgcat
     6361 gcggccggca tggaggaatt gggtagggga caggtttgtg tggattgtat gttgaaggag
     6421 gtggtttgga tggcccgaga atccgagctg ctattccttt gccttcattg gtaatttctt
     6481 caccatcctt cttcagagga gttccaggga aaattccatt gatgcgactg ggtttgcctt
     6541 tgggataggg gtttcctggg aaggttccac acgaagaaat catggcatga ggggctttgc
     6601 agcccgcctt ggaagcctgc tccaggactc tcttgcaccg tttcttctca gacatgttga
     6661 ttctgaataa atcttcaatg ggacgagagt tctgtgcgtc cacaatgttt gaggccaaga
     6721 gctccagagc ggccagcttg tccttactaa aggtgaaaca gctcaagatg ctcaccactt
     6781 ctgctgggtg gacggccacc attttatgct ggagtgcttt catggccttc agctgtggct
     6841 cggcccagga gaagtacctt agcagatcaa ccacctgttc gctggagaag tacccatgaa
     6901 catactcaat tgcttttaat ttatattccg ttaaaacagc ctttcgaatt tcatccagca
     6961 ccgtctcaaa ggactttttg tccatggtga agggggcggc cgcggagcct gcttttttgt
     7021 acaaacttgt tgattcgagg aattctttgc caaaatgatg agacagcaca ataaccagca
     7081 cgttgcccag gagctgtagg aaaaagaaga aggcatgaac atggttagca gaggctctag
     7141 agccgccggt cacacgccag aagccgaacc ccgccctgcc ccgtcccccc cgaaggcagc
     7201 cgtccccccg cggacagccc cgaggctgga gagggagaag gggacggcgg cgcggcgacg
     7261 cacgaaggcc ctccccgccc atttccttcc tgccggcgcc gcaccgcttc gccccgcgcc
     7321 cgctagaggg ggtgcggcgg cgcctcccag atttcggctc cgcacagatt tgggacaaag
     7381 aaagtccctg cgccctctcg cacgattacc ataaaaggca atggctgcgg ctcgccgcgc
     7441 ctcgacagcc gccggcgctc cgggggccgc cgcgccccyc ccccgagccc yccccggccc
     7501 gmggcggccc cgccccgccc ggcacccccm cctgccgcca ccccccgccc ggcacggcga
     7561 gccccgcgcc acgccccgta cggagccccg cacccgaagc cgggccgtgc tcagcaactc
     7621 ggggaggggg gtgcaggggg ggttgcagcc cgaccgacgc gcccacaccc cctgctcacc
     7681 cccccacgca cacaccccgc acgcagcctt tgttcccctc gcagcccccc ccgcaccgcg
     7741 gggcaccgcc cccggccgcg ctcccctcgc gcacactgcg gagcgcacaa agccccgcgc
     7801 cgcgcccgca gcgctcacag ccgccgggca gcgcggagcc gcacgcggcg ctccccacgc
     7861 acacacacac gcacgcaccc cccgagccgc tccccccgca caaagggccc tcccggagcc
     7921 cctcaaggct ttcacgcagc cacagaaaag aaacaagccg tcattaaacc aagcgctaat
     7981 tacagcccgg aggagaaggg ccgtcccgcc cgctcacctg tgggagtaac gcggtcagtc
     8041 agagccgggg cgggcggcgc gaggcggcgg cggagcgggg cacggggcga aggcagcgcg
     8101 cagcgactcc cgcccgccgc gcgcttcgct ttttataggg ccgccgccgc cgccgcctcg
     8161 ccataaaagg aaactttcgg agcgcgccgc tctgattggc tgccgccgca cctctccscc
     8221 tcsccccscc ccscccctcs cccccccccc cccccccygg cgcgcscccc cccccccccc
     8281 cgcccccatc gctgcacaaa ataattaaaa aataaataaa tacaaaattg ggggtgggga
     8341 ggggggggag atggggagag tgaagcagaa cgtggggctc acctcgacca tggtaatagc
     8401 gatgactaat acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc
     8461 ataatgccag gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg
     8521 atacacttga tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca
     8581 atggaaagtc cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg
     8641 ggggtcgttg ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc
     8701 catatatggg ctatgaacta atgaccccgt aattgattac tattaataac tagtcaataa
     8761 tcaatgtcga gggccgcgat atctataaca agaaaatata tatataataa gttatcacgt
     8821 aagtagaaca tgaaataaca atataattat cgtatgagtt aaatcttaaa agtcacgtaa
     8881 aagataatca tgcgtcattt tgactcacgc ggtcgttata gttcaaaatc agtgacactt
     8941 accgcattga caagcacgcc tcacgggagc tccaagcggc gactgagatg tcctaaatgc
     9001 acagcgacgg attcgcgcta tttagaaaga gagagcaata tttcaagaat gcatgcgtca
     9061 attttacgca gactatcttt ctagggttaa ggtacccaat tcgccctata gtgagtcgta
     9121 ttacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     9181 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     9241 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     9301 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     9361 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     9421 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     9481 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     9541 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     9601 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     9661 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     9721 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     9781 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     9841 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     9901 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     9961 aaagtaaaag atgctgaaga tcagt
//
