LOCUS       sequence-45077-e         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Mark WY, Liao JC, Lu Y, Ayed A, Laister R, Szymczyna B, Chakrabartty
            A, Arrowsmith CH
  TITLE     Characterization of segments from the central region of BRCA1: an 
            intrinsically disordered scaffold for multiple protein-protein and 
            protein-DNA interactions?
  JOURNAL   J Mol Biol. 2005 Jan 14;345(2):275-87.
  PUBMED    15571721
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol 
            Biol."; date: "2005-01-14"; volume: "345(2)"; pages: "275-87"
COMMENT     Sequence Label: BRCA1-(540-758)
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(680..697)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(707..724)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             743..765
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 agcagccgga tccttaccac accctttctc cacttaacat gagatctttg gggtcttcag
       61 cattattaga cactttaact gtttctagtt tctcttcttt ttcttctctt ggaaggctag
      121 gattgacaaa ttctttaagt tcactggtat ttgaacactt agtaaaagaa ccaggtgcat
      181 ttgttaactt cagctctggg aaagtatcgc tgtcatgtct tttacttgtc tgttcatttg
      241 gcttgttact cttcttggct ccagttgcag gttctttacc ttccatgagt tgtaggtttc
      301 tgctgtgcct gactggcatt tggttgtact tttttttctt tatctcttca ctgctagaac
      361 aactatcaat ttgcaattca gtacaattag gtgggcttag atttctactg actactagtt
      421 caagcgcatg aatatgcctg gtagaagact tcctcctcag cctattcttt ttaggtgctt
      481 ttgaattgtg gatatttaat tcgagttcca tattgcttat actgctgctt ataggttcag
      541 ctttcgtttt gaaagcagat tctttttcga gtgattctat tgggttagga tttttctcat
      601 tctgaataga atcacctttt gttttattct catgaccact attagtaata ttcatcactt
      661 gaccattctg ctccatatgg ctgccgcgcg gcaccaggcc gctgctgtga tgatgatgat
      721 gatggctgct gcccatggta tatctccttc ttaaagttaa acaaaattat ttctagaggg
      781 g
//
