LOCUS       sequence-450773-       11942 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Adhesion module, creates 'seed' at telomere.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11942)
  AUTHORS   Strom AR, Kim Y, Zhao H, Chang YC, Orlovsky ND, Kosmrlj A, Storm C, 
            Brangwynne CP
  TITLE     Condensate interfacial forces reposition DNA loci and probe 
            chromatin viscoelasticity.
  JOURNAL   Cell. 2024 Aug 14:S0092-8674(24)00828-6. doi: 
            10.1016/j.cell.2024.07.034.
  PUBMED    39168125
REFERENCE   2  (bases 1 to 11942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Aug 14:S0092-8674(24)00828-6. doi: 10.1016/j.cell.2024.07.034."
COMMENT     Sequence Label: FUS-miRFP670-TRF1
FEATURES             Location/Qualifiers
     source          1..11942
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(830..850)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        835..1164
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1015..1150
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          2298..2363
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2493..2513
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2938..3072
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2975..2994)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3029..3048
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             3240..3873
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3920..4045
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4542..4775
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4960..5004
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5289..5406
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5559..5966
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             6421..6432
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             6722..7666
                     /codon_start=1
                     /product="bright monomeric near-infrared fluorescent 
                     protein engineered from a bacterial phytochrome, with an 
                     emission maximum of 670 nm (Shcherbakova et al., 2016)"
                     /label=miRFP670
                     /label=ORF frame 1
                     /translation="MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDH
                     RVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTE
                     YCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG
                     YDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSTVPQMARQLYVRQRVRVLVDVT
                     YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPCHLQFLKDMGVRATLAVSLVVGGKLWGLV
                     VCHHYLPRFIRFELRAICKRLAERIATRITALES"
     misc_feature    9045..9633
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9098..9118)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9516..9527)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9636..9652)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9637..9653)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9743..9976
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(10001..10019)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(10002..10019)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(10309..10328)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(10542..10561)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10661..10683
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10721..10739)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10807..10911
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10912..10980,10981..11772)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGTLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11130..11149)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt
      841 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
      901 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
      961 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     1021 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     1081 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     1141 tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg agatatgttt
     1201 gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg cggactcatg
     1261 tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta ttttcccctc
     1321 gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg aaaaatacat
     1381 cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac tgatgccttc
     1441 tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg
     1501 gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta cgatcacgac
     1561 aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt
     1621 attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa
     1681 gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt
     1741 gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt cgtattcgtc
     1801 ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt tgttcttttt
     1861 aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat ccatgacaca
     1921 ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct
     1981 agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac
     2041 catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg gcattgaccc
     2101 acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac gtaccgacga
     2161 tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg agtgggcccc
     2221 ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag
     2281 agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga
     2341 ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt
     2401 ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg
     2461 aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc
     2521 ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa
     2581 ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa
     2641 ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat cataacatac
     2701 tgttttttct tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat
     2761 tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg
     2821 ccttgactag agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     2881 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     2941 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     3001 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     3061 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
     3121 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
     3181 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt
     3241 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
     3301 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
     3361 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
     3421 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
     3481 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
     3541 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
     3601 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
     3661 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
     3721 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     3781 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     3841 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
     3901 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     3961 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     4021 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     4081 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     4141 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     4201 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     4261 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     4321 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     4381 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     4441 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     4501 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     4561 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     4621 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     4681 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     4741 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     4801 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     4861 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     4921 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     4981 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     5041 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     5101 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     5161 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     5221 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccaaat ggcagtattc
     5281 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     5341 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     5401 aattttcggg tttattacag ggacagcaga gatccagttt ggatcgataa gcttgatatc
     5461 gaattcctgc agccccgata aaataaaaga ttttatttag tctccagaaa aaggggggaa
     5521 tgaaagaccc cacctgtagg tttggcaagc tagctgcagt aacgccattt tgcaaggcat
     5581 ggaaaaatac caaaccaaga atagagaagt tcagatcaag ggcgggtaca tgaaaatagc
     5641 taacgttggg ccaaacagga tatctgcggt gagcagtttc ggccccggcc cggggccaag
     5701 aacagatggt caccgcagtt tcggccccgg cccgaggcca agaacagatg gtccccagat
     5761 atggcccaac cctcagcagt ttcttaagac ccatcagatg tttccaggct cccccaagga
     5821 cctgaaatga ccctgcgcct tatttgaatt aaccaatcag cctgcttctc gcttctgttc
     5881 gcgcgcttct gcttcccgag ctctataaaa gagctcacaa cccctcactc ggcgcgccag
     5941 tcctccgaca gactgagtcg cccggggggg atctggagct ctcgagaatt ctcacgcgtc
     6001 aagtggagca aggcaggtgg acagtggatc cggagctacc atggcctcaa acgattatac
     6061 ccaacaagca acccaaagct atggggccta ccccacccag cccgggcagg gctattccca
     6121 gcagagcagt cagccctacg gacagcagag ttacagtggt tatagccagt ccacggacac
     6181 ttcaggctat ggccagagca gctattcttc ttatggccag agccagaaca caggctatgg
     6241 aactcagtca actccccagg gatatggctc gactggcggc tatggcagta gccagagctc
     6301 ccaatcgtct tacgggcagc agtcctccta tcctggctat ggccagcagc cagctcccag
     6361 cagcacctcg ggaagttacg gtagcagttc tcagagcagc agctatgggc agccccagag
     6421 tgggagctac agccagcagc ctagctatgg tggacagcag caaagctatg gacagcagca
     6481 aagctataat ccccctcagg gctatggaca gcagaaccag tacaacagca gcagtggtgg
     6541 tggaggtgga ggtggaggtg gaggtaacta tggccaagat caatcctcca tgagtagtgg
     6601 tggtggcagt ggtggcggtt atggcaatca agaccagagt ggtggaggtg gcagcggtgg
     6661 ctatggacag caggaccgtg gaggatctgg ctccggttct gcttcaggat ccggatcagg
     6721 tatggtagca ggtcatgcct ctggcagccc cgcattcggg accgcctctc attcgaattg
     6781 cgaacatgaa gagatccacc tcgccggctc gatccagccg catggcgcgc ttctggtcgt
     6841 cagcgaacat gatcatcgcg tcatccaggc cagcgccaac gccgcggaat ttctgaatct
     6901 cggaagcgta ctcggcgttc cgctcgccga gatcgacggc gatctgttga tcaagatcct
     6961 gccgcatctc gatcccaccg ccgaaggcat gccggtcgcg gtgcgctgcc ggatcggcaa
     7021 tccctctacg gagtactgcg gtctgatgca tcggcctccg gaaggcgggc tgatcatcga
     7081 actcgaacgt gccggcccgt cgatcgatct gtcaggcacg ctggcgccgg cgctggagcg
     7141 gatccgcacg gcgggttcac tgcgcgcgct gtgcgatgac accgtgctgc tgtttcagca
     7201 gtgcaccggc tacgaccggg tgatggtgta tcgtttcgat gagcaaggcc acggcctggt
     7261 attctccgag tgccatgtgc ctgggctcga atcctatttc ggcaaccgct atccgtcgtc
     7321 gactgtcccg cagatggcgc ggcagctgta cgtgcggcag cgcgtccgcg tgctggtcga
     7381 cgtcacctat cagccggtgc cgctggagcc gcggctgtcg ccgctgaccg ggcgcgatct
     7441 cgacatgtcg ggctgcttcc tgcgctcgat gtcgccgtgc catctgcagt tcctgaagga
     7501 catgggcgtg cgcgccaccc tggcggtgtc gctggtggtc ggcggcaagc tgtggggcct
     7561 ggttgtctgt caccattatc tgccgcgctt catccgtttc gagctgcggg cgatctgcaa
     7621 acggctcgcc gaaaggatcg cgacgcggat caccgcgctt gagagctccg gaggcggcgg
     7681 atccggcgga tctggaatgg cggaggatgt ttcctcagcg gccccgagcc cgcggggctg
     7741 tgcggatggt agggatgccg accctactga ggagcagatg gcagaaacag agagaaacga
     7801 cgaggagcag ttcgaatgcc aggaactgct cgagtgccag gtgcaggtgg gggcccccga
     7861 ggaggaggag gaggaggagg aggacgcggg cctggtggcc gaggccgagg ccgtggctgc
     7921 cggctggatg ctcgatttcc tctgcctctc tctttgccga gctttccgcg acggccgctc
     7981 cgaggacttc cgcaggaccc gcaacagcgc agaggctatt attcatggac tatccagtct
     8041 aacagcttgc cagttgagaa cgatatacat atgtcagttt ttgacaagaa ttgcagcagg
     8101 aaaaaccctt gatgcacagt ttgaaaatga tgaacgaatt acacccttgg aatcagccct
     8161 gatgatttgg ggttcaattg aaaaggaaca tgacaaactt catgaagaaa tacagaattt
     8221 aattaaaatt caggctatag ctgtttgtat ggaaaatggc aactttaaag aagcagaaga
     8281 agtctttgaa agaatatttg gtgatccaaa ttctcatatg cctttcaaaa gcaaattgct
     8341 tatgataatc tctcagaaag atacatttca ttcctttttt caacacttca gctacaacca
     8401 catgatggag aaaattaaga gttatgtgaa ttatgtgcta agtgaaaaat catcaacctt
     8461 tctaatgaag gcagcggcaa aagtagtaga aagcaaaagg acaagaacaa taacttctca
     8521 agataaacct agtggtaatg atgttgaaat ggaaactgaa gctaatttgg atacaagaaa
     8581 aagtgttagt gacaaacagt ctgcggtaac tgaatcctca gagggtacag tatccttatt
     8641 gaggtctcac aagaatcttt tcttatctaa gttgcaacat ggaacccagc aacaagacct
     8701 taataagaaa gaaagaagag taggaactcc tcaaagtaca aaaaagaaaa aagaaagcag
     8761 aagagccact gaaagcagaa tacctgtttc aaagagtcag ccggtaactc ctgaaaaaca
     8821 tcgagctaga aaaagacagg catggctttg ggaagaagac aagaatttga gatctggcgt
     8881 gaggaaatat ggagagggaa actggtctaa aatactgttg cattataaat tcaacaaccg
     8941 gacaagtgtc atgttaaaag acagatggag gaccatgaag aaactaaaac tgatttcctc
     9001 agacagcgaa gacggcatgc aagcttgata tcaagcttat cgataatcaa cctctggatt
     9061 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     9121 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     9181 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     9241 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     9301 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     9361 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     9421 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     9481 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     9541 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     9601 cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgagggaatt
     9661 aattcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     9721 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     9781 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     9841 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     9901 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     9961 tggaaaatct ctagcagcat ctagaattaa ttccgtgtat tctatagtgt cacctaaatc
    10021 gtatgtgtat gatacataag gttatgtatt aattgtagcc gcgttctaac gacaatatgt
    10081 acaagcctaa ttgtgtagca tctggcttac tgaagcagac cctatcatct ctctcgtaaa
    10141 ctgccgtcag agtcggtttg gttggacgaa ccttctgagt ttctggtaac gccgtcccgc
    10201 acccggaaat ggtcagcgaa ccaatcagca gggtcatcgc tagccagatc ctctacgccg
    10261 gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg
    10321 acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg
    10381 tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac
    10441 cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc
    10501 aggagtcgca taagggagag cgtcgaatgg tgcactctca gtacaatctg ctctgatgcc
    10561 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
    10621 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
    10681 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
    10741 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
    10801 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
    10861 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
    10921 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
    10981 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
    11041 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
    11101 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
    11161 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
    11221 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
    11281 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
    11341 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
    11401 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
    11461 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
    11521 caattaatag actggatgga ggcggataaa gttgcaggaa cacttctgcg ctcggccctt
    11581 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
    11641 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
    11701 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
    11761 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
    11821 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
    11881 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
    11941 tc
//
