LOCUS       sequence-45078-e         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Mills E, Truong K
  TITLE     Ca2+-mediated synthetic biosystems offer protein design versatility,
            signal specificity, and pathway rewiring.
  JOURNAL   Chem Biol. 2011 Dec 23;18(12):1611-9.
  PUBMED    22195563
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Chem 
            Biol."; date: "2011-12-23"; volume: "18(12)"; pages: "1611-9"
COMMENT     Sequence Label: pLyn-CaRQ
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     198..220
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     282..301
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     343..362
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        343..361
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    362..386
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        402..511
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
ORIGIN
        1 agtgaaccgt cagatcacta gatgctttat tgcggtagtt tatcacagtt aaattgctaa
       61 cgccagtctc gaacttaacg tgcagaagtt ggtcgtgagg cactgggcag gtaagtatcg
      121 ggccctttgt gcggggggag cggctcgggg ctgtccgcgg ggggacggct gccttcgggg
      181 gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc
      241 taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc tggttattgt
      301 gctgtctcat cattttggca aagaattgga tcggaccgaa attaatacga ctcactatag
      361 gggaattgtg agcggataac aattccccgg agttaatccg ggacctttaa ttcaacccaa
      421 cacaatatat tatagttaaa taagaattat tatcaaatca tttgtatatt aattaaaata
      481 ctatactgta aattacattt tatttacaat caaaggagat ataccatggg ctgcatcaag
      541 agcaagggca aggacagcgc cactagtgag cagattgcag agttcaaaga agccttctca
      601 ttattcgaca aggatgggga cggcaccatc accacaaagg aacttggcac cgttatgagg
      661 tcgcttggac aaaacccaac ggaagcagaa ttgcaggata tgatcaatga agtcgatgct
      721 gatggcaatg gaacgattta ctttcctgaa tttcttacta tgatggctag aaaaatgaag
      781 gacacagaca gcgaagagga aatccgagaa gcattccgtg tttttgacaa ggatgggaac
      841 ggctacatca gcgctgctga attacgtcac gtcatgac
//
