LOCUS       sequence-45081-e         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Mark WY, Liao JC, Lu Y, Ayed A, Laister R, Szymczyna B, Chakrabartty
            A, Arrowsmith CH
  TITLE     Characterization of segments from the central region of BRCA1: an 
            intrinsically disordered scaffold for multiple protein-protein and 
            protein-DNA interactions?
  JOURNAL   J Mol Biol. 2005 Jan 14;345(2):275-87.
  PUBMED    15571721
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol 
            Biol."; date: "2005-01-14"; volume: "345(2)"; pages: "275-87"
COMMENT     Sequence Label: BRCA1-(894-1057)
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             63..80
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             90..107
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(659..677)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      673..720
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tggggacatt ccctctgaat attttgttta ctttaagaag gagatatacc atgggcagca
       61 gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat atgaaccaaa
      121 gtccaaaagt cacttttgaa tgtgaacaaa aggaagaaaa tcaaggaaag aatgagtcta
      181 atatcaagcc tgtacagaca gttaatatca ctgcaggctt tcctgtggtt ggtcagaaag
      241 ataagccagt tgataatgcc aaatgtagta tcaaaggagg ctctaggttt tgtctatcat
      301 ctcagttcag aggcaacgaa actggactca ttactccaaa taaacatgga cttttacaaa
      361 acccatatcg tataccacca ctttttccca tcaagtcatt tgttaaaact aaatgtaaga
      421 aaaatctgct agaggaaaac tttgaggaac attcaatgtc acctgaaaga gaaatgggaa
      481 atgagaacat tccaagtaca gtgagcacaa ttagccgtaa taacattaga gaaaatgttt
      541 ttaaagaagc cagctcaagc aatattaatg aagtaggttc cagtactaat gaagtgggct
      601 ccagttaagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc
      661 gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg
      721 ctgaaaggag gaactatatc cggatatccc gcaagaggcc cggcagtacc ggcataacca
      781 agcctatgcc tacagcatcc ag
//
