LOCUS       sequence-45082-e         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Mills E, Truong K
  TITLE     Ca2+-mediated synthetic biosystems offer protein design versatility,
            signal specificity, and pathway rewiring.
  JOURNAL   Chem Biol. 2011 Dec 23;18(12):1611-9.
  PUBMED    22195563
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Chem 
            Biol."; date: "2011-12-23"; volume: "18(12)"; pages: "1611-9"
COMMENT     Sequence Label: Stim1-CaRQ
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     215..237
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     299..318
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     360..379
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        360..378
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    379..403
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        419..528
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
ORIGIN
        1 ggctttagcg agtcgttagt gaaccgtcag atcactagat gctttattgc ggtagtttat
       61 cacagttaaa ttgctaacgc cagtctcgaa cttaacgtgc agaagttggt cgtgaggcac
      121 tgggcaggta agtatcgggc cctttgtgcg gggggagcgg ctcggggctg tccgcggggg
      181 gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc
      241 ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc
      301 aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattggatcg gaccgaaatt
      361 aatacgactc actatagggg aattgtgagc ggataacaat tccccggagt taatccggga
      421 cctttaattc aacccaacac aatatattat agttaaataa gaattattat caaatcattt
      481 gtatattaat taaaatacta tactgtaaat tacattttat ttacaatcaa aggagatata
      541 ccatgggcct gactagtgga tccgatgtat gcgtccgtct tgccctgtgg ctcctctggg
      601 gactcctcct gcaccagggc cagagcctca gccatagtca cagtgagaag gcgacaggaa
      661 ccagctcggg ggccaactct gaggagtcca ctgcagcaga gttttgccga attgacaagc
      721 ccctgtgtca cagtgaggat gagaaactca gcttcgaggc agtccgtaac atccacaaac
      781 tgatggacga tgatgccaat ggtgatgtgg atgtgga
//
