LOCUS       sequence-45083-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Wong SS, Kotera I, Mills E, Suzuki H, Truong K
  TITLE     Split-intein mediated re-assembly of genetically encoded Ca(2+) 
            indicators.
  JOURNAL   Cell Calcium. 2011 Nov 29.
  PUBMED    22133610
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Calcium. 2011 Nov 29."
COMMENT     Sequence Label: nGCaMP2-NpuDnaEn
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    16..40
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        56..165
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             202..219
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             223..255
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(259..267,268..282)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      289..298
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             310..366
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     579..600
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgactcact ataggggaat tgtgagcgga taacaattcc ccggagttaa tccgggacct
       61 ttaattcaac ccaacacaat atattatagt taaataagaa ttattatcaa atcatttgta
      121 tattaattaa aatactatac tgtaaattac attttattta caatcaaagg agatatacca
      181 tgtgccacca tgcggggttc tcatcatcat catcatcatg gtatggctag catgactggt
      241 ggacagcaaa tgggtcggga tctgtacgac gatgacgata aggatctcgc caccatggtc
      301 gactcatcac gtcgtaagtg gaataagaca ggtcacgcag tcagagctat aggtcggctg
      361 agctcactcg agaacgtcta tatcatggcc gacaagcaga agaacggcat caaggcgaac
      421 ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc tcgcctacca ctaccagcag
      481 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
      541 tccaaacttt cgaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
      601 accgccgccg ggatcactct cggcatggac gagctgtaca agggcggatc ctgtttaagc
      661 tatgaaacgg aaatattgac agtagaatat ggattattac cgattggtaa aattgtagaa
      721 aagcgcatcg aatgtactgt ttatagcgtt gataataatg gaaatattta tacacaacct
      781 gtagcacaat ggcacgatcg cggagaacaa gaggtgtttg agtattgttt ggaagatgg
//
