LOCUS       sequence-450833-        7573 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning of genes of interest with a C-terminal mECFP fusion;
            generation of stable cells with doxycycline inducible expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7573)
  TITLE     Zaja lab plasmids
REFERENCE   2  (bases 1 to 7573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA5-FRT-TOgw-C-mECFP
FEATURES             Location/Qualifiers
     source          1..7573
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(105..122)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    111..335
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(468..487)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    619..666
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             674..1696
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             674..1696
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    1826..1947
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1863..1882)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1917..1936
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1996..2012)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1996..2012)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2009..2031)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2020..2036
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2044..2050,2051..2068,2069..2074))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2089..2110
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2227..2244)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2398..2986)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2478..2497)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3157..3948,3949..4017))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3780..3799
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4018..4122)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4197..4216)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4388..4767
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4768..4971
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4922..4942
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4973..4991
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4994..5012
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     5022..5046
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    5139..5263
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(5288..5293,5294..5311,5312..5318)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             5372..6031
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(5438..5457)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             6373..6678
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     6557..6576
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(6719..6843)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(6857..6859,6860..7573)
                     /codon_start=1
                     /product="enhanced CFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mECFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(6899..6920)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7160..7179)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7507..7528
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtaccgagct cggatccact agtccagtgt ggtggaattc tgcagatatc cagcacagtg
       61 gcggccgctc gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc
      121 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
      181 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
      241 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
      301 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
      361 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
      421 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
      481 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
      541 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
      601 tgatggttca cgtacctaga agttcctatt ccgaagttcc tattctctag aaagtatagg
      661 aacttccttg gccaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga
      721 aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt
      781 cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt
      841 ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt
      901 gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg
      961 tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga
     1021 ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg
     1081 accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc
     1141 ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc
     1201 tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc
     1261 ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg
     1321 gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc
     1381 gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc
     1441 aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag
     1501 cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt
     1561 ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg
     1621 gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc
     1681 gagggcaaag gaatagcacg tactacgaga tttcgattcc accgccgcct tctatgaaag
     1741 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     1801 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     1861 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     1921 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     1981 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     2041 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     2101 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     2161 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     2221 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     2281 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     2341 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     2401 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     2461 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     2521 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     2581 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     2641 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     2701 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     2761 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     2821 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     2881 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     2941 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     3001 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     3061 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     3121 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     3181 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     3241 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     3301 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     3361 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     3421 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     3481 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     3541 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     3601 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     3661 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     3721 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     3781 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     3841 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     3901 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     3961 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     4021 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     4081 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     4141 gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca
     4201 atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc
     4261 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
     4321 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
     4381 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
     4441 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     4501 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     4561 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     4621 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     4681 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     4741 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     4801 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     4861 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     4921 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctccctatc
     4981 agtgatagag atctccctat cagtgataga gatcgtcgac gagctcgttt agtgaaccgt
     5041 cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga
     5101 tccagcctcc ggactctagc gtttaaactt aagctatcac aagtttgtac aaaaaagctg
     5161 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
     5221 agactacata atactgtaaa acacaacata tccagtcact atggcggccg cattaggcac
     5281 cccaggcttt acactttatg cttccggctc gtataatgtg tggattttga gttaggatcc
     5341 gtcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac
     5401 cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca
     5461 atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa
     5521 aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca
     5581 tccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc
     5641 ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca
     5701 cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa
     5761 cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg
     5821 ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt
     5881 tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca
     5941 ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca
     6001 gtactgcgat gagtggcagg gcggggcgta aacgcgtgga tccggcttac taaaagccag
     6061 ataacagtat gcgtatttgc gcgctgattt ttgcggtata agaatatata ctgatatgta
     6121 tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc gtattacagt gacagttgac
     6181 agcgacagct atcagttgct caaggcatat atgatgtcaa tatctccggt ctggtaagca
     6241 caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg
     6301 aagggatggc tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct gacgagaaca
     6361 ggggctggtg aaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg
     6421 tttgtggatg tacagagtga tattattgac acgcccgggc gacggatggt gatccccctg
     6481 gccagtgcac gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc
     6541 ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc
     6601 ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg
     6661 atgttctggg gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca
     6721 tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta
     6781 atttaatata ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt
     6841 ggtgatagct tggtacgtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     6901 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     6961 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     7021 gccctggccc accctcgtga ccaccctgac ctggggcgtg cagtgcttca gccgctaccc
     7081 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     7141 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     7201 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     7261 catcctgggg cacaagctgg agtacaacta catcagccac aacgtctata tcaccgccga
     7321 caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg aggacggcag
     7381 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     7441 gcccgacaac cactacctga gcacccagtc caagctgagc aaagacccca acgagaagcg
     7501 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     7561 gctgtacaag tga
//
