LOCUS       sequence-45084-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Visanji NP, Wislet-Gendebien S, Oschipok LW, Zhang G, Aubert I, 
            Fraser PE, Tandon A
  TITLE     Effect of Ser-129 phosphorylation on interaction of alpha-synuclein 
            with synaptic and cellular membranes.
  JOURNAL   J Biol Chem. 2011 Oct 14;286(41):35863-73. Epub 2011 Aug 17.
  PUBMED    21849493
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2011-10-14"; volume: "286(41)"; pages: "35863-73. 
            Epub 2011 Aug 17"
COMMENT     Sequence Label: pWPI/hPLK2WT/Neo
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    47..164
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             385..402
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gaatttcgac atttaaattt aattaatctc gacggtatcg gttaactttt aaaagaaaag
       61 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
      121 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttatcgat cacgagacta
      181 gcctcgaggt ttcatatgta cagaggatcc gcgaccatgg agcttttgcg gactatcacc
      241 taccagccag ccgccagcac caaaatgtgc gagcaggcgc tgggcaaggg ttgcggagcg
      301 gactcgaaga agaagcggcc gccgcagccc cccgaggaat cgcagccacc tcagtcccag
      361 gcgcaagtgc ccccggcggc ccctcaccac catcaccacc attcgcactc ggggccggag
      421 atctcgcgga ttatcgtcga ccccacgact gggaagcgct actgccgggg caaagtgctg
      481 ggaaagggtg gctttgcaaa atgttacgag atgacagatt tgacaaataa caaagtctac
      541 gccgcaaaaa ttattcctca cagcagagta gctaaacctc atcaaaggga aaagattgac
      601 aaagaaatag agcttcacag aattcttcat cataagcatg tagtgcagtt ttaccactac
      661 ttcgaggaca aagaaaacat ttacattctc ttggaatact gcagtagaag gtcaatggct
      721 catattttga aagcaagaaa ggtgttgaca gagccagaag ttcgatacta cctcaggcag
      781 attgtgtctg gactgaaaat accttcatga acaagaaatc ttgcacagag atctcaaact
      841 agggaacttt tttttttaat gaagcc
//
