LOCUS       sequence-450843-        2039 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3' homology arm for S. pombe genomic integration. Part type 7 
            following the YeastToolkit MoClo grammar..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Hebra T, Smrckova H, Elkatmis B, Prevorovsky M, Pluskal T
  TITLE     POMBOX: A Fission Yeast Cloning Toolkit for Molecular and Synthetic 
            Biology.
  JOURNAL   ACS Synth Biol. 2023 Nov 22. doi: 10.1021/acssynbio.3c00529.
  PUBMED    37991801
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 22. doi: 10.1021/acssynbio.3c00529."
COMMENT     Sequence Label: pPOM034_P7_3""Lys3
FEATURES             Location/Qualifiers
     source          1..2039
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(24..58)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(129..788)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     703..722
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      970..1558
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1459..1478
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggaggaccga tgagaccaga ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca
       61 aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg
      121 atatcaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
      181 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
      241 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
      301 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgaaacgaaa
      361 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
      421 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
      481 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
      541 accagctcac cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca
      601 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
      661 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
      721 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
      781 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
      841 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgcccgat caatcatgac
      901 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
      961 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1021 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1081 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     1141 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1201 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1261 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1321 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     1381 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     1441 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     1501 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     1561 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     1621 ctttcctgcg ttatcccctg attctgtgga taaccgtagt cggtctcaga gtatggaacg
     1681 attgaatact gtgctactct tttaccaatt gcagtctggc ttaaagcaat gaggttgtaa
     1741 ttaaatggct tgagagctag aacaaacacc ttttacaatc cttgtttact ggttttgata
     1801 gtaaattcgg ttcgcttgtg gtgtacatta tattttttag gctagtcgtg attttttgat
     1861 aatatcaata ttcttagatt tgagacattc atgcttttta atttgatcaa tcgtttgtat
     1921 cttcattaat tagtaggtgg tttgatagaa catttagttg cttgtttgac tacaaatatc
     1981 tgcagaatgg attcggttaa taaaaaaaat gtttgaggaa ttaattttcg ctttggagt
//
