LOCUS       sequence-45085-e        4359 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Seeliger JC, Topp S, Sogi KM, Previti ML, Gallivan JP, Bertozzi CR
  TITLE     A riboswitch-based inducible gene expression system for 
            mycobacteria.
  JOURNAL   PLoS One. 2012;7(1):e29266. Epub 2012 Jan 18.
  PUBMED    22279533
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(1)"; pages: "e29266. Epub 2012 Jan 18"
COMMENT     Sequence Label: pRibo BsaHind
FEATURES             Location/Qualifiers
     source          1..4359
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      2258..2304
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(2454..2473)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2465..3280
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2538..2557)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3611..4199
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4100..4119
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagcccacca gctccgtaag ttcgggcgct gtgtggctcg tacccgcgca ttcaggcggc
       61 agggggtcta acgggtctaa ggcggcgtgt acggccgcca cagcggctct cagcggcccg
      121 gaaacgtcct cgaaacgacg catgtgttcc tcctggttgg tacaggtggt tgggggtgct
      181 cggctgtcgc tggtgttcca ccaccagggc tcgacgggag agcgggggag tgtgcagttg
      241 tggggtggcc cctcagcgaa atatctgact tggagctcgt gtcggaccat acaccggtga
      301 ttaatcgtgg tctactacca agcgtgagcc acgtcgccga cgaatttgag cagctctggc
      361 tgccgtactg gccgctggca agcgacgatc tgctcgaggg gatctaccgc caaagccgcg
      421 cgtcggccct aggccgccgg tacatcgagg cgaacccaac agcgctggca aacctgctgg
      481 tcgtggacgt agaccatcca gacgcagcgc tccgagcgct cagcgcccgg gggtcccatc
      541 cgctgcccaa cgcgatcgtg ggcaatcgcg ccaacggcca cgcacacgca gtgtgggcac
      601 tcaacgcccc tgttccacgc accgaatacg cgcggcgtaa gccgctcgca tacatggcgg
      661 cgtgcgccga aggccttcgg cgcgccgtcg atggcgaccg cagttactca ggcctcatga
      721 ccaaaaaccc cggccacatc gcctgggaaa cggaatggct ccactcagat ctctacacac
      781 tcagccacat cgaggccgag ctcggcgcga acatgccacc gccgcgctgg cgtcagcaga
      841 ccacgtacaa agcggctccg acgccgctag ggcggaattg cgcactgttc gattccgtca
      901 ggttgtgggc ctatcgtccc gccctcatgc ggatctacct gccgacccgg aacgtggacg
      961 gactcggccg cgcgatctat gccgagtgcc acgcgcgaaa cgccgaattt ccgtgcaacg
     1021 acgtgtgtcc cggaccgcta ccggacagcg aggtccgcgc catcgccaac agcatttggc
     1081 gttggatcac aaccaagtcg cgcatttggg cggacgggat cgtggtctac gaggccacac
     1141 tcagtgcgcg ccagtcggcc atctcgcgga agggcgcagc agcgcgcacg gcggcgagca
     1201 cagttgcgcg gcgcgcaaag tccgcgtcag ccatggaggc attgctatga gcgacggcta
     1261 cagcgacggc tacaaccggc agccgactgt ccgcaaaaag cggcgcgtga ccgccgccga
     1321 aggcgctcga atcaccggac tatccgaacg ccacgtcgtc cggctcgtgg cgcaggaacg
     1381 cagcgagtgg ctcgccgagc aggctgcacg ccgcgaacgc atccgcgcct atcacgacga
     1441 cgagggccac tcttggccgc aaacggccaa acatttcggg ctgcatctgg acaccgttaa
     1501 gcgactcggc tatcgggcga ggaaagagcg tgcggcagaa caggaagcgg ctcaaaaggc
     1561 ccacaacgaa gccgacaatc caccgctgtt ctaacgcaat tggggagcgg gtgtcgcggg
     1621 ggttccgtgg ggggttccgt tgcaacgggt cggacaggta aaagtcctgg tagacgctag
     1681 ttttctggtt tgggccatgc ctgtctcgtt gcgtgtttcg ttgcgcccgt tttgaatacc
     1741 agccagacga gacggggttc tacgaatctt ggtcgatacc aagccatttc cgctgaatat
     1801 cggggagctc accgccagaa tcggtggttg tggtgatgta cgtggcgaac tccgttgtag
     1861 tgcctgtggt ggcatccgtg gcgcggccgc ggtaccagat ctttaaatct agaggtgacc
     1921 acaacgcgcc cgctttgatc ggggacgtct gcggccgacc atttacgggt cttgttgtcg
     1981 ttggcggtca tgggccgaac atactcaccc ggatcggagg gccgaggaca aggtcgaacg
     2041 aggggcatga cccggtgcgg ggcttcttgc actcggcata ggcgagtgct aagaataacg
     2101 ttggcactcg cgaccgggat acgactcact ataggtaccg gtgataccag catcgtcttg
     2161 atgcccttgg cagcaccctg ctaaggaggc aacaagatgg agaccaagct tatcgatgtc
     2221 gacgtagtta actagcgtac gatcgactgc caggcatcaa ataaaacgaa aggctcagtc
     2281 gaaagactgg gcctttcgtt ttatgccatc atggccgcgg tgatcagcta gccacctgac
     2341 gtcggggggg ggggaaagcc acgttgtgtc tcaaaatctc tgatgttaca ttgcacaaga
     2401 taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg
     2461 tgttatgagc catattcaac gggaaacgtc ttgctcgagg ccgcgattaa attccaacat
     2521 ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac
     2581 aatctatcgc ttgtatggga agccccatgc gccagagttg tttctgaaac atggcaaagg
     2641 tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat
     2701 gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac
     2761 tgcgatcccc gggaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa
     2821 tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg
     2881 tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg
     2941 tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg
     3001 gaaagaaatg cataatcttt tgccattctc accggattca gtcgtcactc atggtgattt
     3061 ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg
     3121 agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt
     3181 ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa
     3241 taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt
     3301 gtaacactgg cagagcatta cgctgacttg acgggacggc ggctttgttg aataaatcga
     3361 acttttgctg agttgaagga tcagatcacg catcttcccg acaacgcaga ccgttccgtg
     3421 gcaaagcaaa agttcaaaat caccaactgg tccacctaca acaaagctct caccaaccgt
     3481 ggctccctca ctttctggct ggatgatggg gcgattcagg cctggtatga gtcagcaaca
     3541 ccttcttcac gaggcagacc tcactagttc catgagcgtc agaccccgta gaaaagatca
     3601 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     3661 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     3721 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     3781 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     3841 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     3901 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     3961 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     4021 ttcccgaggg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     4081 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     4141 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     4201 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     4261 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     4321 ataccgctcg ccgcagccga acgaccgagc gcaacgcgt
//
