LOCUS       sequence-45087-e         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Wong SS, Kotera I, Mills E, Suzuki H, Truong K
  TITLE     Split-intein mediated re-assembly of genetically encoded Ca(2+) 
            indicators.
  JOURNAL   Cell Calcium. 2011 Nov 29.
  PUBMED    22133610
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Calcium. 2011 Nov 29."
COMMENT     Sequence Label: nGCaMP2-NpuDnaEn
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             378..401
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(491..510)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    556..611
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(610..629)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     terminator      699..746
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gcaggacgag ctgtcagggc ggatcctgtt taagctatga aacggaaata ttgacagtag
       61 aatatggatt attaccgatt ggtaaaattg tagaaaagcg catcgaatgt actgtttata
      121 gcgttgataa taatggaaat atttatacac aacctgtagc acaatggcac gatcgcggag
      181 aacaagaggt gtttgagtat tgtttggaag atggttcatt gattcgggca acaaaagacc
      241 ataagtttat gactgttgat ggtcaaatgt tgccaattga tgaaatattt gaacgtgaat
      301 tggatttgat gcgggttgat aatttgccga attagaagga gatatacatg gctagcctgt
      361 ttaaactgta actcgagcac caccatcacc atcaccatca ctaagtgatt aacctcaggt
      421 gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca
      481 ctgagatcga tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc
      541 atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt
      601 tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt
      661 atttggttta gagtttggca acatatgccc atatgtaact agcataaccc cttggggcct
      721 ctaaacgggt cttgaggggt tttttgctga aagcatgcgg aggaaat
//
