LOCUS       sequence-45090-e         671 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Visanji NP, Wislet-Gendebien S, Oschipok LW, Zhang G, Aubert I, 
            Fraser PE, Tandon A
  TITLE     Effect of Ser-129 phosphorylation on interaction of alpha-synuclein 
            with synaptic and cellular membranes.
  JOURNAL   J Biol Chem. 2011 Oct 14;286(41):35863-73. Epub 2011 Aug 17.
  PUBMED    21849493
REFERENCE   2  (bases 1 to 671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2011-10-14"; volume: "286(41)"; pages: "35863-73. 
            Epub 2011 Aug 17"
COMMENT     Sequence Label: pWPI/hPLK2WT/Neo
FEATURES             Location/Qualifiers
     source          1..671
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    37..588
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    39..606
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(198..217)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     427..444
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(646..663)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cgcggaaact gcgtgcatcc atcttgttca tcatggtatt atcatcgtgt ttttcaaagg
       61 aaaaccacgt ccccgtggtt cggggggcct agacgttttt taacctcgac taaacacatg
      121 taaagcatgt gcaccgaggc cccagatcag atcccataca atggggtacc ttctgggcat
      181 ccttcagccc cttgttgaat acgcttgagg agagccattt gactctttcc acaactatcc
      241 aactcacaac gtggcactgg ggttgtgccg cctttgcagg tgtatcttat acacgtggct
      301 tttggccgca gaggcacctg tcgccaggtg gggggttccg ctgcctgcaa agggtcgcta
      361 cagacgttgt ttgtcttcaa gaagcttcca gaggaactgc ttccttcacg acattcaaca
      421 gaccttgcat tcctttggcg agaggggaaa gacccctagg aatgctcgtc aagaagacag
      481 ggccaggttt ccgggccctc acattgccaa aagacggcaa tatggtggaa aataacatat
      541 agacaaacgc acaccggcct tattccaagc ggcttcggcc agtaacgtta gggggggggg
      601 ggcggaattc ctgcagcccg taatcccggg actagtggat cctcaatggt gatggtgatg
      661 atgaccggta c
//
