LOCUS       sequence-45091-e        4386 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Seeliger JC, Topp S, Sogi KM, Previti ML, Gallivan JP, Bertozzi CR
  TITLE     A riboswitch-based inducible gene expression system for 
            mycobacteria.
  JOURNAL   PLoS One. 2012;7(1):e29266. Epub 2012 Jan 18.
  PUBMED    22279533
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(1)"; pages: "e29266. Epub 2012 Jan 18"
COMMENT     Sequence Label: pRibo EcoHind
FEATURES             Location/Qualifiers
     source          1..4386
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      2285..2331
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(2481..2500)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2492..3307
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2565..2584)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3638..4226
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4127..4146
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagcccacca gctccgtaag ttcgggcgct gtgtggctcg tacccgcgca ttcaggcggc
       61 agggggtcta acgggtctaa ggcggcgtgt acggccgcca cagcggctct cagcggcccg
      121 gaaacgtcct cgaaacgacg catgtgttcc tcctggttgg tacaggtggt tgggggtgct
      181 cggctgtcgc tggtgttcca ccaccagggc tcgacgggag agcgggggag tgtgcagttg
      241 tggggtggcc cctcagcgaa atatctgact tggagctcgt gtcggaccat acaccggtga
      301 ttaatcgtgg tctactacca agcgtgagcc acgtcgccga cgaatttgag cagctctggc
      361 tgccgtactg gccgctggca agcgacgatc tgctcgaggg gatctaccgc caaagccgcg
      421 cgtcggccct aggccgccgg tacatcgagg cgaacccaac agcgctggca aacctgctgg
      481 tcgtggacgt agaccatcca gacgcagcgc tccgagcgct cagcgcccgg gggtcccatc
      541 cgctgcccaa cgcgatcgtg ggcaatcgcg ccaacggcca cgcacacgca gtgtgggcac
      601 tcaacgcccc tgttccacgc accgaatacg cgcggcgtaa gccgctcgca tacatggcgg
      661 cgtgcgccga aggccttcgg cgcgccgtcg atggcgaccg cagttactca ggcctcatga
      721 ccaaaaaccc cggccacatc gcctgggaaa cggaatggct ccactcagat ctctacacac
      781 tcagccacat cgaggccgag ctcggcgcga acatgccacc gccgcgctgg cgtcagcaga
      841 ccacgtacaa agcggctccg acgccgctag ggcggaattg cgcactgttc gattccgtca
      901 ggttgtgggc ctatcttccc gccctcatgc ggatctacct gccgacccgg aacgtggacg
      961 gactcggccg cgcgatctat gccgagtgcc acgcgcgaaa cgccgaattt ccgtgcaacg
     1021 acgtgtgtcc cggaccgcta ccggacagcg aggtccgcgc catcgccaac agcatttggc
     1081 gttggatcac aaccaagtcg cgcatttggg cggacgggat cgtggtctac gaggccacac
     1141 tcagtgcgcg ccagtcggcc atctcgcgga agggcgcagc agcgcgcacg gcggcgagca
     1201 cagttgcgcg gcgcgcaaag tccgcgtcag ccatggaggc attgctatga gcgacggcta
     1261 cagcgacggc tacaaccggc agccgactgt ccgcaaaaag cggcgcgtga ccgccgccga
     1321 aggcgctcga atcaccggac tatccgaacg ccacgtcgtc cggctcgtgg cgcaggaacg
     1381 cagcgagtgg ctcgccgagc aggctgcacg ccgcgaacgc atccgcgcct atcacgacga
     1441 cgagggccac tcttggccgc aaacggccaa acatttcggg ctgcatctgg acaccgttaa
     1501 gcgactcggc tatcgggcga ggaaagagcg tgcggcagaa caggaagcgg ctcaaaaggc
     1561 ccacaacgaa gccgacaatc caccgctgtt ctaacgcaat tggggagcgg gtgtcgcggg
     1621 ggttccgtgg ggggttccgt tgcaacgggt cggacaggta aaagtcctgg tagacgctag
     1681 ttttctggtt tgggccatgc ctgtctcgtt gcgtgtttcg ttgcgcccgt tttgaatacc
     1741 agccagacga gacggggttc tacgaatctt ggtcgatacc aagccatttc cgctgaatat
     1801 cggggagctc accgccagaa tcggtggttg tggtgatgta cgtggcgaac tccgttgtag
     1861 tgcctgtggt ggcatccgtg gcgcggccgc ggtaccagat ctttaaatct agaggtgacc
     1921 acaacgcgcc cgctttgatc ggggacgtct gcggccgacc atttacgggt cttgttgtcg
     1981 ttggcggtca tgggccgaac atactcaccc ggatcggagg gccgaggaca aggtcgaacg
     2041 aggggcatga cccggtgcgg ggcttcttgc actcggcata ggcgagtgct aagaataacg
     2101 ttggcactcg cgaccgggat acgactcact ataggtaccg gtgataccag catcgtcttg
     2161 atgcccttgg cagcaccctg ctaaggaggc aacaagatgg ccaagacaat tgcggatcca
     2221 gctgcagaat tcaagcttat cgatgtcgac gtagttaact agcgtacgat cgactgccag
     2281 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tgccatcatg
     2341 gccgcggtga tcagctagcc acctgacgtc gggggggggg gaaagccacg ttgtgtctca
     2401 aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc
     2461 tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcttg
     2521 ctcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg
     2581 cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc cccatgcgcc
     2641 agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt
     2701 cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac
     2761 tcctgatgat gcatggttac tcaccactgc gatccccggg aaaacagcat tccaggtatt
     2821 agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg
     2881 gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc
     2941 tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg
     3001 taatggctgg cctgttgaac aagtctggaa agaaatgcat aatcttttgc cattctcacc
     3061 ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa
     3121 attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc
     3181 catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa
     3241 atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt
     3301 tttctaatca gaattggtta attggttgta acactggcag agcattacgc tgacttgacg
     3361 ggacggcggc tttgttgaat aaatcgaact tttgctgagt tgaaggatca gatcacgcat
     3421 cttcccgaca acgcagaccg ttccgtggca aagcaaaagt tcaaaatcac caactggtcc
     3481 acctacaaca aagctctcac caaccgtggc tccctcactt tctggctgga tgatggggcg
     3541 attcaggcct ggtatgagtc agcaacacct tcttcacgag gcagacctca ctagttccat
     3601 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     3661 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     3721 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     3781 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     3841 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     3901 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     3961 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     4021 agcgtgagct atgagaaagc gccacgcttc ccgaggggag aaaggcggac aggtatccgg
     4081 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     4141 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     4201 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     4261 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     4321 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     4381 acgcgt
//
