LOCUS       sequence-450913-       11779 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rpt5Q49TAG base (3xFLAG-Rpt1, Rpt2, 6xHis-Rpt3, Rpt4, Rpt5Q49TAG, 
            Rpt6).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11779)
  AUTHORS   Bard JAM, Bashore C, Dong KC, Martin A
  TITLE     The 26S Proteasome Utilizes a Kinetic Gateway to Prioritize 
            Substrate Degradation.
  JOURNAL   Cell. 2019 Apr 4;177(2):286-298.e15. doi: 
            10.1016/j.cell.2019.02.031. Epub 2019 Mar 28.
  PUBMED    30929903
REFERENCE   2  (bases 1 to 11779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.02.031"; journalName: "Cell"; date: "2019-04-4-
            4"; volume: "177"; issue: "2"; pages: "286-298.e15"
COMMENT     Sequence Label: pAM89
FEATURES             Location/Qualifiers
     source          1..11779
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        99..117
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    118..142
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             157..179
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             192..215
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        1647..1665
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1666..1690
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        3062..3080
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3081..3105
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3120..3142
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3161..3178
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        4492..4510
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4511..4535
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        5900..5918
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5919..5943
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        7221..7239
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    7240..7264
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             7279..7301
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      8641..8688
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(8912..9727)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8912..9727)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9635..9654
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(9728..9819)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9837..10469)
                     /direction=LEFT
                     /label=ColA ori
                     /note="Plasmids containing the ColA origin of replication 
                     can be propagated in E. coli cells that contain additional 
                     plasmids with compatible origins."
     primer_bind     complement(9982..10001)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10667..11749)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     11711..11730
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(join(11750..11779,1..48))
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac
       61 gctctccctt atgcgactcc taaatcaccg gtggaaatta atacgactca ctatagggga
      121 attgtgagcg gataacaatt cccctctaga aataattttg tttaacttta agaaggagat
      181 ataccatggc cgattacaaa gatgacgatg acaaaagctc tggccatatg ccaccaaaag
      241 aagactggga aaaatacaag gcacctttgg aagacgatga taaaaaacct gatgatgaca
      301 agatcgtacc cttaacagaa ggtgacattc aagtcttaaa atcatacggt gcagctccat
      361 acgcagccaa attaaagcaa actgagaatg acttaaaaga cattgaagcg agaatcaaag
      421 aaaaggccgg tgtgaaagaa agcgatacag ggttagcacc ctcccatcta tgggatatta
      481 tgggtgatag gcagagactg ggcgaagaac atcccttaca agtcgctcgt tgcacgaaga
      541 tcattaaggg gaatggtgaa agtgacgaaa ctacgacaga taataataac agcggcaata
      601 gcaatagtaa tagcaatcaa cagtctactg atgcagacga agacgatgaa gatgccaagt
      661 acgtgataaa tcttaagcaa attgccaaat ttgtcgtcgg attaggtgaa cgtgtttctc
      721 caacagatat agaagaaggt atgcgtgtag gtgtcgatag gtcgaaatat aatattgaac
      781 ttccattgcc tccaaggatt gacccatcag ttactatgat gaccgttgaa gaaaagcctg
      841 acgttactta tagcgatgtc ggtggctgta aagaccaaat tgaaaagctg agagaagttg
      901 tcgagctgcc cttattatct ccagaaagat ttgctactct tggtattgat ccaccaaagg
      961 gtatcttatt atatgggcca cctggtactg gtaagacatt atgtgctcgt gctgttgcta
     1021 atagaactga tgcaactttt attagggtca ttgggtctga gctagtgcaa aaatatgtcg
     1081 gtgaaggtgc tcgtatggtt agagaattat ttgaaatggc tcgtaccaaa aaggcttgca
     1141 ttatattctt cgatgaaatc gacgctgttg gtggtgctcg ttttgatgat ggtgctggtg
     1201 gtgataatga agttcaaaga actatgttag aactgattac acagttagac gggtttgacc
     1261 ctcgtggtaa cattaaagtg atgtttgcca ccaataggcc caacacttta gatccagctt
     1321 tattgcgccc gggtagaatt gatcgtaagg tagagttttc attacctgac ttggaaggtc
     1381 gtgcaaatat cttccgtatt cactctaaat caatgagtgt ggaacgtggc atcagatggg
     1441 aacttatctc cagactgtgt ccaaactcta ccggtgcaga attaagatct gtttgcacag
     1501 aagccggtat gtttgccatt agagcaagaa gaaaggtggc tactgaaaag gatttcctga
     1561 aggctgttga taaagttatt agcggataca agaagtttag ttccacatcg cgttatatgc
     1621 aatataattg aaatactgca ggaaattaat acgactcact ataggggaat tgtgagcgga
     1681 taacaattcc ccatcttagt atattagtta agtataagaa ggagatatac atatgggaca
     1741 aggtgtatca tctggacaag acaaaaagaa gaagaaggga tctaatcaga aacccaaata
     1801 tgaacctccc gttcaatcta aatttggtcg taagaaaaga aagggaggtc cagctactgc
     1861 tgaaaaatta cccaacatat atccaagtac tcgttgtaag ttaaagcttc taaggatgga
     1921 acgaattaaa gaccatttgt tattagaaga agaatttgtc tctaactctg aaattttaaa
     1981 accctttgaa aagaagcagg aggaggaaaa gaaacaattg gaagaaatac gtggaaatcc
     2041 attgagcatt gggacccttg aagaaattat agacgatgat catgcgatag tgacaagtcc
     2101 aactatgcct gattattatg tatctatctt atcctttgtc gataaagagt tactagagcc
     2161 tggatgttct gtgttattgc atcataaaac catgtccatc gtaggtgttt tacaggatga
     2221 cgccgatcca atggtttcgg tcatgaaaat ggataagtcg ccaacagaat catacagtga
     2281 tattggtggc ttagaatctc aaattcaaga aataaaagag tctgtagagt taccgttaac
     2341 acaccccgaa ctgtatgaag agatgggtat caaacctccc aagggtgtga ttctttatgg
     2401 tgcacccggt acaggtaaga cattgctagc aaaggctgtt gcaaatcaaa catctgccac
     2461 attcttaagg attgttggct cagagttaat tcaaaaatat ttgggggatg gtcctaggtt
     2521 gtgtaggcaa attttcaaag tagcgggtga gaatgcacca agtattgtat ttattgatga
     2581 aatagacgcc attggtacta aaagatatga ctccaatagt ggtggtgaga gagaaattca
     2641 gagaaccatg ctggaattgc tgaaccagct ggacgggttc gacgacagag gggatgtgaa
     2701 ggtgattatg gccactaaca aaatcgagac tcttgaccca gcattaatca ggcctgggag
     2761 aattgatcgt aaaatcttat ttgaaaaccc tgatctaagt actaagaaaa aaattttagg
     2821 gattcacact tccaagatga atttaagtga ggacgttaat cttgaaactt tagtaacaac
     2881 aaaggatgat ttgtcaggcg ccgatatcca ggcaatgtgt acagaagcag gtttgttagc
     2941 ccttagagag agaagaatgc aagttacagc ggaggatttt aaacaagcca aggagagagt
     3001 catgaaaaat aaagttgagg aaaatttaga aggtttatac ttgtgaataa gtcgacgaaa
     3061 ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctct agaaataatt
     3121 ttgtttaact ttaagaagga gatataccat gggcagcagc catcatcatc atcatcattc
     3181 tagtcatatg gaggaactag gtattgtgac acctgtcgaa aaggcagtcg aagaaaaacc
     3241 agccgtcaaa tcatacgcat cattgttggc tcaactcaat ggtacagtca acaataacag
     3301 tgccctgtct aatgtgaatt ctgatatata tttcaaattg aagaaattag aaaaagagta
     3361 tgaactgctg actttgcaag aagactatat taaggacgaa caacgccatt tgaaacgtga
     3421 attgaagaga gctcaagaag aagtaaagag aatccagtcg gttccattag ttatcgggca
     3481 attcttagaa cctattgatc agaacacagg tattgtttct agtaccacag gtatgagtta
     3541 tgtcgttaga atattatcaa ccctagaccg tgaactatta aagccatcta tgtctgtagc
     3601 tttacatcgt cattcaaatg ctttagttga catcctacca cctgattccg actctagcat
     3661 atccgtaatg ggcgaaaacg aaaaacctga cgtcacttat gcagatgttg gtgggttaga
     3721 tatgcaaaag caagaaatca gggaagccgt agagctgcca ttagttcagg ctgatctata
     3781 cgagcaaatc ggtattgacc ctcctagggg tgttctatta tatggtccac caggtaccgg
     3841 taagacgatg cttgttaaag ccgtcgccaa tagtacaaaa gctgcattca taagagttaa
     3901 tggctccgaa tttgttcaca aatatcttgg tgaaggtcca agaatggttc gtgacgtctt
     3961 tagactcgcc agggaaaatg ctccatccat tatattcata gatgaggtag attccattgc
     4021 cacaaagcgt tttgatgctc aaacgggttc cgatcgtgaa gtacagcgta tcctgattga
     4081 gttgttgaca caaatggatg gtttcgatca atccaccaac gttaaagtca tcatggctac
     4141 aaacagggca gacacattgg acccagcttt gctgcgtccg ggcagattag atagaaagat
     4201 tgagtttccc tcacttcgtg ataggcgtga acgtcgtttg attttcggta ccattgccag
     4261 taagatgtcc cttgccccag aagcggactt agactcgctt attattcgta acgactctct
     4321 atcaggtgct gttattgcag cgatcatgca ggaagctggt ttaagagcag tgaggaaaaa
     4381 cagatatgtc atcttacaaa gcgatctgga agaagcttac gccactcaag tcaagacaga
     4441 taataccgtc gataaattcg acttctacaa atgaaccagc ggccgcgaaa ttaatacgac
     4501 tcactatagg ggaattgtga gcggataaca attccccatc ttagtatatt agttaagtat
     4561 aagaaggaga tatacatatg gccaccttgg aagaattgga tgctcaaact ttaccaggag
     4621 acgatgaatt agatcaagag attttgaatc tttcgacgca ggagcttcaa acaagagcca
     4681 agctcttgga caatgaaatt cgtattttta gatctgaatt gtagagactg tcccatgaaa
     4741 acaacgttat gctcgagaag attaaggaca ataaggaaaa aatcaagaat aatagacagt
     4801 taccgtacct tgtggctaac gttgtcgagg tcatggatat gaatgagatt gaagacaaag
     4861 aaaatagtga atctactaca caaggtggta atgtcaactt agataacacc gctgttggca
     4921 aagctgctgt agtcaagact tcttctagac agactgtttt cttgcccatg gtcggtttag
     4981 tagatcctga taaacttaag ccaaatgacc tggtaggtgt gaataaagac tcgtatttga
     5041 ttttggatac tttaccctct gaatttgatt ctcgtgtaaa agctatggag gtagatgaaa
     5101 aacccacaga aacttactcg gacgtgggtg ggttggataa acagattgaa gagctggtgg
     5161 aggccattgt gttacctatg aaacgtgcag ataaattcaa agatatggga attagagcgc
     5221 ccaagggtgc cctaatgtac gggcctccgg gtacaggtaa aactttatta gctcgtgctt
     5281 gcgcggctca gacgaatgct acttttttga agctggcagc acctcaattg gtacaaatgt
     5341 atatcggtga aggtgctaaa ctagtccgcg atgcttttgc actggccaag gaaaaggcac
     5401 caactattat tttcattgac gaattggatg ccattggtac taagagattt gattccgaaa
     5461 aatccggtga tagggaagtg caaagaacca tgctggaatt attgaatcaa ttggatggct
     5521 ttagctccga tgatcgtgtc aaagtcttgg cggctaccaa tagagttgat gttttagatc
     5581 ctgctttgtt aagatcagga agattggata gaaaaattga attcccactc ccatccgaag
     5641 actcgagagc tcaaatttta caaattcatt caagaaaaat gaccactgat gatgatatta
     5701 actggcaaga acttgccagg tctactgatg agtttaatgg tgcccagtta aaggcggtaa
     5761 ctgtggaggc aggtatgatt gccttaagga atgggcaatc ttccgttaaa cacgaagatt
     5821 tcgttgaggg tataagtgaa gttcaagcaa gaaaatcgaa atcggtatcc ttttatgcat
     5881 aacaacggcc ggccgaaatt aatacgactc actatagggg aattgtgagc ggataacaat
     5941 tccccatctt agtatattag ttaagtataa gaaggagata tacatatgac agctgctgta
     6001 acatcctcca atatagtatt agaaacccac gaaagtggta tcaaaccata ttttgaacag
     6061 aagatccaag aaacagaatt aaagatccgc tccaaaacag aaaatgttcg cagactggaa
     6121 gctcaaagga atgcattgaa tgacaaagta cgttttatca aggacgaact gcgtctatta
     6181 caggaacctg gatcttatgt gggtgaagtt ataaagatcg tgtctgacaa aaaagttctc
     6241 gttaaagtgc aacctgaggg aaaatacatc gtggatgttg caaaagatat aaacgtgaag
     6301 gacttaaagg catctcaaag agtttgtcta aggagtgact cttatatgtt gcataaagtt
     6361 cttgagaata aggctgaccc actagtttcg ttgatgatgg tggaaaaagt tcctgattcc
     6421 acatacgata tggttggtgg tttgacaaag caaataaagg agattaaaga agttattgaa
     6481 ttgcccgtaa aacatcctga actttttgaa agtttgggta ttgcgcaacc aaagggtgtc
     6541 atcttatatg gcccccctgg tacagggaaa accttattgg caagagctgt cgcacatcac
     6601 actgattgta aattcatcag ggtcagtggt gcggaactgg tacaaaagta tatcggcgaa
     6661 ggttcccgta tggtccgtga gctgtttgtg atggctagag aacatgctcc ctcaattatc
     6721 tttatggatg aaatcgattc cattggctct actcgtgtag aaggttctgg tggtggtgat
     6781 tcagaagttc aaagaacaat gttagaacta ctaaaccaat tggacgggtt tgaaacttct
     6841 aaaaatatca agatcataat ggccacgaat agactagata ttctagatcc agcacttttg
     6901 cgcccgggta gaatagatag gaagattgaa tttccacctc caagtgtcgc agctagagct
     6961 gaaattttaa gaatccattc cagaaaaatg aatctaactc gtggtatcaa cttgagaaag
     7021 gttgctgaaa agatgaacgg ttgttctggt gccgatgtca aaggtgtctg tacagaagct
     7081 ggtatgtatg ctttaagaga aagaagaata cacgttactc aagaagactt cgaactcgct
     7141 gtgggtaagg ttatgaacaa gaaccaagaa acggccattt ctgtcgccaa gctgttcaag
     7201 tgaaggatta attaagaaat taatacgact cactataggg gaattgtgag cggataacaa
     7261 ttcccctcta gaaataattt tgtttaactt taagaaggag atataccatg ggcagtgaag
     7321 aacaggaccc tttattagca ggattggggg aaacgtccgg cgacaatcac acccaacagt
     7381 cacacgaaca acaaccagaa caaccacagg aaacggaaga acatcatgaa gaggagcctt
     7441 ctagagtgga tcctgaacag gaggcccata acaaggcctt gaatcaattc aagagaaagc
     7501 tgctagaaca cagaaggtat gatgatcaat tgaagcagcg ccgtcaaaat atacgagatc
     7561 tagagaagtt atatgacaag actgaaaacg acataaaagc tttgcaaagt attggacaat
     7621 tgattggcga agtcatgaag gaattatctg aagagaaata tatagtgaag gcctcttcgg
     7681 gcccacgtta tattgtcgga gttaggaatt cggtagatcg ctctaaatta aagaaagggg
     7741 tgagagtcac cttagatatc accactttga ctataatgag aattttgccc agagagacag
     7801 atccactggt gtataatatg accagttttg aacaaggtga aatcactttt gatggtattg
     7861 gtggtttgac tgagcaaatc agagaattaa gggaggttat agaattacca ttgaagaacc
     7921 cagaaatttt ccagagggtc ggtatcaaac ctccaaaggg tgtgctacta tatggtcccc
     7981 ctggtactgg taaaacttta ctagcgaagg cagttgctgc tacgattgga gccaacttca
     8041 ttttctctcc agcgtctggt attgtggata aatacattgg tgagtctgct cgtattatta
     8101 gagaaatgtt tgcatatgca aaggaacacg aaccttgcat catttttatg gatgaagtgg
     8161 atgctattgg tggtagaaga ttcagtgaag gtacttcggc agaccgtgaa attcaacgta
     8221 cgttgatgga gttgttgacc cagatggatg gttttgataa tcttggacag actaaaatca
     8281 tcatggctac gaatagacca gatactttag atcctgcttt gttgaggccc ggtagactag
     8341 acaggaaagt tgaaatccca ttgccaaatg aggccggcag attagaaatt ttcaaaattc
     8401 atactgcaaa agtcaaaaaa acaggggaat ttgacttcga ggctgctgta aaaatgagtg
     8461 atggtttcaa tggtgctgat attcgtaact gtgcaactga agcaggtttt ttcgccataa
     8521 gagatgaccg tgatcacatc aaccctgatg atttaatgaa agccgtaaga aaagtagctg
     8581 aagttaagaa attggaaggc actatagaat accaaaaatt atgagaaggc tgagcaataa
     8641 ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaacctcag
     8701 gcatttgaga agcacacggt cacactgctt ccggtagtca ataaaccggt aaaccagcaa
     8761 tagacataag cggctattta acgaccctgc cctgaaccga cgacaagctg acgaccgcgg
     8821 aagtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     8881 ttcaaatatg tatccgctca tgaattaatt cttagaaaaa ctcatcgagc atcaaatgaa
     8941 actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta
     9001 atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg
     9061 cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt
     9121 tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagtttat
     9181 gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg
     9241 catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat acgcggtcgc
     9301 tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg
     9361 catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat gctgttttgc
     9421 cggggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg
     9481 tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat
     9541 tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca
     9601 atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata
     9661 aatcagcatc catgttggaa tttaatcgcg gcctagagca agacgtttcc cgttgaatat
     9721 ggctcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     9781 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggcatgcta gcgcagaaac
     9841 gtcctagaag atgccaggag gatacttagc agagagacaa taaggccgga gcgaagccgt
     9901 ttttccatag gctccgcccc cctgacgaac atcacgaaat ctgacgctca aatcagtggt
     9961 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctgatggc tccctcttgc
    10021 gctctcctgt tcccgtcctg cggcgtccgt gttgtggtgg aggctttacc caaatcacca
    10081 cgtcccgttc cgtgtagaca gttcgctcca agctgggctg tgtgcaagaa ccccccgttc
    10141 agcccgactg ctgcgcctta tccggtaact atcatcttga gtccaacccg gaaagacacg
    10201 acaaaacgcc actggcagca gccattggta actgagaatt agtggattta gatatcgaga
    10261 gtcttgaagt ggtggcctaa cagaggctac actgaaagga cagtatttgg tatctgcgct
    10321 ccactaaagc cagttaccag gttaagcagt tccccaactg acttaacctt cgatcaaacc
    10381 gcctccccag gcggtttttt cgtttacaga gcaggatatt acgacgataa aaggatctca
    10441 agaagatcct ttacggattc ccgacaccat cactctagat ttcagtgcaa tttatctctt
    10501 caaatgtagc acctgaagtc agccccatac gatataagtt gtaattctca tgttagtcat
    10561 gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga
    10621 tcccggtgcc taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt
    10681 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
    10741 cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct
    10801 gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc
    10861 ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt
    10921 cggtatcgtc gtatcccact accgagatgt ccgcaccaac gcgcagcccg gactcggtaa
    10981 tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga
    11041 tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt
    11101 cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac
    11161 gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca
    11221 atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt
    11281 tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt
    11341 ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt
    11401 gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg
    11461 acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg
    11521 acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg
    11581 ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt
    11641 tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat
    11701 aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc
    11761 tgaattgact ctcttccgg
//
