LOCUS       sequence-450925-       12684 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Replicating base vector for constructing cpf1/CRISPR editing 
            plasmids.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12684)
  AUTHORS   Ungerer J, Pakrasi HB
  TITLE     Cpf1 Is A Versatile Tool for CRISPR Genome Editing Across Diverse 
            Species of Cyanobacteria.
  JOURNAL   Sci Rep. 2016 Dec 21;6:39681. doi: 10.1038/srep39681.
  PUBMED    28000776
REFERENCE   2  (bases 1 to 12684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-12-21"; volume: "6:39681. doi"; pages: " 
            10.1038/srep39681"
COMMENT     Sequence Label: pSL2680
FEATURES             Location/Qualifiers
     source          1..12684
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..395
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     primer_bind     complement(1242..1261)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1253..2068
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             1253..2068
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1326..1345)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     protein_bind    2764..2785
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2800..2805,2806..2823,2824..2830)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2838..2854
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2843..2865
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2862..2878
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2862..2878
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2874..3170
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSG"
     primer_bind     2897..2917
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2899..2917
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2964..2983)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2965..2983)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2993..3010)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2993..3009)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3002..3024)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        complement(3238..3272)
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     promoter        complement(join(7196..7201,7202..7219,7220..7225))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(7329..7348)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(7858..8709)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(8696..9535)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(10046..11017)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            12256..12343
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
ORIGIN
        1 ggctgcacac gcgcccccac ccttcgggta gggggaaagg ccgctaaagc ggctaaaagc
       61 gctccagcgt atttctgcgg ggtttggtgt ggggtttagc gggctttgcc cgcctttccc
      121 cctgccgcgc agcggtgggg cggtgtgtag cctagcgcag cgaatagacc agctatccgg
      181 cctctggccg ggcatattgg gcaagggcag cagcgcccca caagggcgct gataaccgcg
      241 cctagtggat tattcttaga taatcatgga tggatttttc caacaccccg ccagcccccg
      301 cccctgctgg gtttgcaggt ttgggggcgt gacagttatt gcaggggttc gtgacagtta
      361 ttgcaggggg gcgtgacagt tattgcaggg gttcgtgaca gttagtacgg gagtgacggg
      421 cactggctgg caatgtctag caacggcagg catttcggct gagggtaaaa gaactttccg
      481 ctaagcgata gactgtatgt aaacacagta ttgcaaggac gcggaacatg cctcatgtgg
      541 cggccaggac ggccagccgg gatcgggata ctggtcgtta ccagagccac cgacccgagc
      601 aaacccttct ctatcagatc gttgacgagt attacccggc attcgctgcg cttatggcag
      661 agcagggaaa ggaattgccg ggctatgtgc aacgggaatt tgaagaattt ctccaatgcg
      721 ggcggctgga gcatggcttt ctacgggttc gctgcgagtc ttgccacgcc gagcacctgg
      781 tcgctttcag ctgtaatccg ggcagcgcaa cggaacattc atcagtgtaa aaatggaatc
      841 aataaagccc tgcgcagcgc gcagggtcag cctgaatacg cgtttaatga ccagcacagt
      901 cgtgatggca aggtcagaat agcgctgagg tctgcctcgt gaagaaggtg ttgctgactc
      961 ataccaggcc tgaatcgccc catcatccag ccagaaagtg agggagccac ggttgatgag
     1021 agctttgttg taggtggacc agttggtgat tttgaacttt tgctttgcca cggaacggtc
     1081 tgcgttgtcg ggaagatgcg tgatctgatc cttcaactca gcaaaagttc gatttattca
     1141 acaaagccac gttgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc
     1201 atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca
     1261 tattcaacgg gaaacgtctt gctcgaggcc gcgattaaat tccaacatgg atgctgattt
     1321 atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt
     1381 gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa
     1441 tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac
     1501 catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg
     1561 gaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc
     1621 gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag
     1681 cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc
     1741 gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca
     1801 taagcttttg ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa
     1861 ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc
     1921 agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt
     1981 acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt
     2041 tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca
     2101 gagcattacg ctgacttgac gggacggcgg ctttgttgaa taaatcgaac ttttgctgag
     2161 ttgaaggatc agatcacgca tcttcccgac aacgcagacc gttccgtggc aaagcaaaag
     2221 ttcaaaatca ccaactggtc cacctacaac aaagctctca tcaaccgtgg ctccctcact
     2281 ttctggctgg atgatggggc gattcaggcc tggtatgagt cagcaacacc ttcttcacga
     2341 ggcagacctc agcgctattc tgaccttgcc atcacgactg tgctggtcat taaacgcgta
     2401 ttcaggctga ccctgcgcgc tgcgcagggc tttattgatt ccatttttac actgatgaat
     2461 gttccgttgc gctgcccgga ttacagatcc tctagagtcg acggtaccaa ctaccgcatt
     2521 aaagctagac aaaaaaatga ccttcataaa tcgctaatct acaacagtag aaattattta
     2581 aagttcttag accggctact aatacaaaag gctctagaca tctacaacag tagaaattat
     2641 ttaaagttct tagactgaat tatctgaagg cacaggaata gtagcatcta caacagtaga
     2701 aattatttaa agttcttaga cttttgcagg tgggtggaaa gcgggcagtg agcgcaacgc
     2761 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     2821 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     2881 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg aggacctcga
     2941 gggggggccc ggtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
     3001 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     3061 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     3121 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggctga cacctgctct
     3181 catctacaac agtagaaatt atttaaagtt cttagacccg tttttgccta aatcagcgct
     3241 agcattatac ctaggactga gctagctgtc aagaattctt agttattcct attctgcacg
     3301 aactcaaaat actcttcatt tttgataacc aaattgagtt ttttgccctc ttgattattt
     3361 ttgatcctac ctagtagcat cagacctttt agcccaatat gataagcacc attggcatca
     3421 gcatcttgag gcatattttt tggcgcctgt cgcgaatcaa agaaattgcc atttacatct
     3481 gctactggtg aaattagata atctaactca gtacctgttt ttgagttacg catttgtaag
     3541 atagtattta ggacactagt tagcttagca aaaaactttt tgtcgctctc accgcaaata
     3601 gctgctttga tacattcgcc atgcccatat tcgatagaat aatcttttag caatttctcc
     3661 aactctttag ttggataaac ttctcgagta tcccaattat gatttttatc tgaatttcta
     3721 aagttaatca atctactccc aaagctagct atagtccact tgcctttggc agccttgtca
     3781 ccaaagtttt tataatcaaa actaaactca aaatagccct tatcaaggtt ataacaaatc
     3841 ttgtcaaact tactaaagaa ctcttgagat ttgctgacac tttcatactt aggatataac
     3901 tgatttacaa aaccagttac aggacaaatt tttgaagtaa aaccagctgg tacatagtag
     3961 ataatacctg tttgtttacc catcttttta aaagtctcaa aaggtgctgt tagctgataa
     4021 gctctaagca ctcccccagt tttatcaaac tcattatctt tgaaaactag atagtttagt
     4081 ttctcaatta gcattttttc taacttttga tagacctgct tctctacctt gaaacgccct
     4141 cttttaaatc caaaatttaa atcctcaaaa accacaatag cattatactc tataactagc
     4201 ttagctattt catgaactac ctgagataga tagccctctt tcatctcttt gatgttattt
     4261 atctttttcc agtctttcct agctgaatcc ctatctttct ctattgcagc aagcttatca
     4321 tggtagtttg ttttcattct atcattacca atgatgttga aagtatcttg tttgatgata
     4381 ttgcctttac catctaccaa agtatagtaa gctaaatgtc tttcacctct atctatactt
     4441 aatatatgaa catcatttgc tttttctttt agcaataaat tgatttcatc attaaactta
     4501 ttagctccac tagatttaaa attgattgta ataggacagt gaaagaaaaa cttatcttca
     4561 gtaaagcgtt tatctttgat taaatcatat tcaaaaacac tctctttttt aggattatct
     4621 ttgtttttat tagctattgc ctctttagct gggtgagtga tttttttagg tattgattgt
     4681 ttacgataaa aaagctctgc ctcaccattt agcttataaa ccacatcttg aagatttctc
     4741 tcatcaaaca gcgctttcca atataaagta tgtagatttg gtcgcccttt gctataagct
     4801 gaaaaatctt tattatagat ttggaatagg tacaatttac cctgattaac tacgctatca
     4861 atatagctct ctgatatatt ttcaaaagtt agtttgtagc cttgattttc aacttctcta
     4921 taaaattcat ctatagaatt atatctttga gtatcagaaa atctaaatcc aaaatctttc
     4981 cactccggat gcttacttat agactgttta taaaaatcta taaattttcg gcaatcttca
     5041 atattaaact caaatttttc atatcctttt tgaggactac cattttttgt atgtgtggaa
     5101 tgatttctta ttctaagtat atcttcacta ggattataaa attttataga tttagcagaa
     5161 aagaaaacct taggtaacat tttatttgcg ccaggtaaaa gtttataaac aattttttta
     5221 taaccctcgc ctttattttc tttgatagct ttatcatcaa atattttgtt atttttctta
     5281 ttcatcacac ccagataata tttatcatct ttgataaata aaattgccgt attgtcaggc
     5341 tctttatttt tatcccaacc attagccaaa gtcgagttct caaaattgag cttaaatttc
     5401 tcatcactat atggcttttg agttatatag tttctaattt tgttataaag aggcactata
     5461 ttcgctagct caaagtagca ctcctcaaat actagataaa aatgctcatc cttgtctaaa
     5521 atatttgcct tatcttctga ctgactaata tgaaatattt ttagtttatg taagagatta
     5581 ttagtttgat ctaaaagatc cttgatagct ttaacatcat cttccgcact agcttgaagt
     5641 aggtcttttt taccttgatt ttgatatttg atagatatct gtgccaaatt gtctttgttt
     5701 tgagctattt catcaaatat catcggaata gccgcaaagt ttgcaagtat ttcttcaaac
     5761 ctacactgtt tatctatatc tctatgctta ttaaattctt ctaaggcaag ctttatagtt
     5821 tctagagata agtattttgc tttttcagtt tttttggcta ttaattcttg ctctttctta
     5881 ctagggttat caagattttt aggtgctatt tgttgagtta tatattctag taccgctgta
     5941 ccaataacac tataatcatc aaaaacttgt tgtgatagat cagtaagaga tttatcattt
     6001 ttaaaataaa ttttactcaa atcaagtttt tgagctttta aatcatcaaa taataaagat
     6061 agtgtttctt taatagattt ttcttctact gttttaaaag ctgctatttg ctcataaaaa
     6121 ctttgcatcg ttgtaactac atcactatca tcttctaact tatcaattac aaaagattta
     6181 gattctgtat cacttaaaat ttgcttaaat aaaacactca ttttatattt tttgagtgtt
     6241 ttatcattta tttgctgtga gtatagattt atatattcat ttataccttt tctctttgta
     6301 ttttcaccat ttacaaattt accaccaata atagtattaa atttagtaat accactttga
     6361 tttagataat tattaaagtt tgctatctca aaaacttcat caagtgaaaa aactctttga
     6421 ttaacttcag atgttttgta gtcaatatca aaggttagct cttctgccaa atctttttta
     6481 atttgttcat agtttatagc ttctggagct ttgtctttta aactctcata cttagcttta
     6541 ttttctagaa atttaggcaa attatcatct actatcctat aaataataga tgtaggaata
     6601 tcattgctac tataaacatt ttttctattt tcatgaaaac ccttaaaata agttgtccaa
     6661 cctttaaaag atttgattat ttctaacgcc tcatctatat ctgtgatatc actattggct
     6721 ttaaatagtt ctataccatt atccttagat tgctttagcc atagaattaa atctgactct
     6781 tgcccttttt tagcatcgat aaggttttga ttaaacaaat tcttaaattt ctctgagtcc
     6841 tttatatatt cagatatttg tttctttatc gtatcttttg cacttttaaa atctttttgt
     6901 agattatcat catcactctt tttaagttta aaataaacat cagaatagtt ttgtaataaa
     6961 tcttcgctaa tacaaaccga acttaatatc tcctctataa aaaactgatg atatttatca
     7021 attatttgtt tagccttttt gtagtcttta gctcttttct catcatctaa aatcaaacct
     7081 cttgctttta tgttttcaag tgttttaccc tgtgggatta actcaaatct tagagtttta
     7141 cttaaactat atttattaac aaattcttga taaattgaca tgataaagac ctcctaacat
     7201 acgagccgga agcataaagt gtaaagtgca cgagtgggtt acatcgaact ggatctcaac
     7261 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     7321 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
     7381 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     7441 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     7501 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     7561 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     7621 ataccaaacg acgagcgtga caccacgatg cctgcaggag cagaagagca tacatctgga
     7681 agcaaagcca ggaaagcggc ctatggagct gtgcggcagc gctcagtagg caatttttca
     7741 aaatattgtt aagccttttc tgagcatggt atttttcatg gtattaccaa ttagcaggaa
     7801 aataagccat tgaatataaa agataaaaat gtcttgttta caatagagtg gggggggtca
     7861 gcctgccgcc ttgggccggg tgatgtcgta cttgcccgcc gcgaactcgg ttaccgtcca
     7921 gcccagcgcg accagctccg gcaacgcctc gcgcacccgc tggcggcgct tgcgcatggt
     7981 cgaaccactg gcctctgacg gccagacata gccgcacaag gtatctatgg aagccttgcc
     8041 ggttttgccg gggtcgatcc agccacacag ccgctggtgc agcaggcggg cggtttcgct
     8101 gtccagcgcc cgcacctcgt ccatgctgat gcgcacatgc tggccgccac ccatgacggc
     8161 ctgcgcgatc aaggggttca gggccacgta caggcgcccg tccgcctcgt cgctggcgta
     8221 ctccgacagc agccgaaacc cctgccgctt gcggccattc tgggcgatga tggatacctt
     8281 ccaaaggcgc tcgatgcagt cctgtatgtg cttgagcgcc ccaccactat cgacctctgc
     8341 cccgatttcc tttgccagcg cccgatagct acctttgacc accatggcat cagcggtgac
     8401 ggcctcccac ttgggttcca ggaacagccg gagctgccgt ccgccttcgg tcttgggttc
     8461 cgggccaagc actaggccat taggcccagc catggccacc agcccttgca ggatgcgcag
     8521 atcatcagcg cccagcggct ccgggccgct gaactcgatc cgcttgccgt cgccgtagtc
     8581 atacgtcacg tccagcttgc tgcgcttgcg ctcgccccgc ttgagggcac ggaacaggcc
     8641 gggggccaga cagtgcgccg ggtcgtgccg gacgtggctg aggctgtgct tgttcttagg
     8701 cttcaccacg gggcaccccc ttgctcttgc gctgcctctc cagcacggcg ggcttgagca
     8761 ccccgccgtc atgccgcctg aaccaccgat cagcgaacgg tgcgccatag ttggccttgc
     8821 tcacaccgaa gcggacgaag aaccggcgct ggtcgtcgtc cacaccccat tcctcggcct
     8881 cggcgctggt catgctcgac aggtaggact gccagcggat gttatcgacc agtaccgagc
     8941 tgccccggct ggcctgctgc tggtcgcctg cgcccatcat ggccgcgccc ttgctggcat
     9001 ggtgcaggaa cacgatagag cacccggtat cggcggcgat ggcctccatg cgaccgatga
     9061 cctgggccat ggggccgctg gcgttttctt cctcgatgtg gaaccggcgc agcgtgtcca
     9121 gcaccatcag gcggcggccc tcggcggcgc gcttgaggcc gtcgaaccac tccggggcca
     9181 tgatgttggg caggctgccg atcagcggct ggatcagcag gccgtcagcc acggcttgcc
     9241 gttcctcggc gctgaggtgc gccccaaggg cgtgcaggcg gtgatgaatg gcggtgggcg
     9301 ggtcttcggc gggcaggtag atcaccgggc cggtgggcag ttcgcccacc tccagcagat
     9361 ccggcccgcc tgcaatctgt gcggccagtt gcagggccag catggattta ccggcaccac
     9421 cgggcgacac cagcgccccg accgtaccgg ccaccatgtt gggcaaaacg tagtccagcg
     9481 gtggcggcgc tgctgcgaac gcctccagaa tattgatagg cttatgggta gccattgatt
     9541 gcctcctttg caggcagttg gtggttaggc gctggcgggg tcactacccc cgccctgcgc
     9601 cgctctgagt tcttccaggc actcgcgcag cgcctcgtat tcgtcgtcgg tcagccagaa
     9661 cttgcgctga cgcatccctt tggccttcat gcgctcggca tatcgcgctt ggcgtacagc
     9721 gtcagggctg gccagcaggt cgccggtctg cttgtccttt tggtctttca tatcagtcac
     9781 cgagaaactt gccggggccg aaaggcttgt cttcgcggaa caaggacaag gtgcagccgt
     9841 caaggttaag gctggccata tcagcgactg aaaagcggcc agcctcggcc ttgtttgacg
     9901 tataaccaaa gccaccgggc aaccaatagc ccttgtcact tttgatcagg tagaccgacc
     9961 ctgaagcgct tttttcgtat tccataaaac ccccttctgt gcgtgagtac tcatagtata
    10021 acaggcgtga gtaccaacgc aagcactaca tgctgaaatc tggcccgccc ctgtccatgc
    10081 ctcgctggcg gggtgccggt gcccgtgcca gctcggcccg cgcaagctgg acgctgggca
    10141 gacccatgac cttgctgacg gtgcgctcga tgtaatccgc ttcgtggccg ggcttgcgct
    10201 ctgccagcgc tgggctggcc tcggccatgg ccttgccgat ttcctcggca ctgcggcccc
    10261 ggctggccag cttctgcgcg gcgataaagt cgcacttgct gaggtcatca ccgaagcgct
    10321 tgaccagccc ggccatctcg ctgcggtact cgtccagcgc cgtgcgccgg tggcggctaa
    10381 gctgccgctc gggcagttcg aggctggcca gcctgcgggc cttctcctgc tgccgctggg
    10441 cctgctcgat ctgctggcca gcctgctgca ccagcgccgg gccagcggtg gcggtcttgc
    10501 ccttggattc acgcagcagc acccacggct gataaccggc gcgggtggtg tgcttgtcct
    10561 tgcggttggt gaagcccgcc aagcggccat agtggcggct gtcggcgctg gccgggtcgg
    10621 cgtcgtactc gctggccagc gtccgggcaa tctgcccccg aagttcaccg cctgcggcgt
    10681 cggccacctt gacccatgcc tgatagttct tcgggctggt ttccactacc agggcaggct
    10741 cccggccctc ggctttcatg tcatccaggt caaactcgct gaggtcgtcc accagcacca
    10801 gaccatgccg ctcctgctcg gcgggcctga tatacacgtc attgccctgg gcattcatcc
    10861 gcttgagcca tggcgtgttc tggagcactt cggcggctga ccattcccgg ttcatcatct
    10921 ggccggtggt ggcgtccctg acgccgatat cgaagcgctc acagcccatg gccttgagct
    10981 gtcggcctat ggcctgcaaa gtcctgtcgt tcttcatcgg gccaccaagc gcagccagat
    11041 cgagccgtcc tcggttgtca gtggcgtcag gtcgagcaag agcaacgatg cgatcagcag
    11101 caccaccgta ggcatcatgg aagccagcat cacggttagc catagcttcc agtgccaccc
    11161 ccgcgacgcg ctccgggcgc tctgcgcggc gctgctcacc tcggcggcta cctcccgcaa
    11221 ctctttggcc agctccaccc atgccgcccc tgtctggcgc tgggctttca gccactccgc
    11281 cgcctgcgcc tcgctggcct gctgggtctg gctcatgacc tgccgggctt cgtcggccag
    11341 tgtcgccatg ctctgggcca gcggttcgat ctgctccgct aactcgttga tgcctctgga
    11401 tttcttcact ctgtcgattg cgttcatggt ctattgcctc ccggtattcc tgtaagtcga
    11461 tgatctgggc gttggcggtg tcgatgttca gggccacgtc tgcccggtcg gtgcggatgc
    11521 cccggccttc catctccacc acgttcggcc ccaggtgaac accgggcagg cgctcgatgc
    11581 cctgcgcctc aagtgttctg tggtcaatgc gggcgtcgtg gccagcccgc tctaatgccc
    11641 ggttggcatg gtcggcccat gcctcgcggg tctgctcaag ccatgccttg ggcttgagcg
    11701 cttcggtctt ctgtgccccg cccttctccg gggtcttgcc gttgtaccgc ttgaaccact
    11761 gagcggcggg ccgctcgatg ccgtcattga tccgctcgga gatcatcagg tggcagtgcg
    11821 ggttctcgcc gccaccggca tggatggcca gcgtatacgg caggcgctcg gcaccggtca
    11881 ggtgctgggc gaactcggac gccagcgcct tctgctggtc gagggtcagc tcgaccggca
    11941 gggcaaattc gacctccttg aacagccgcc cattggcgcg ttcatacagg tcggcagcat
    12001 cccagtagtc ggcgggccgc tcgacgaact ccggcatgtg cccggattcg gcgtgcaaga
    12061 cttcatccat gtcgcgggca tacttgcctt cgcgctggat gtagtcggcc ttggccctgg
    12121 ccgattggcc gcccgacctg ctgccggttt tcgccgtaag gtgataaatc gccatgctgc
    12181 ctcgctgttg cttttgcttt tcggctccat gcaatggccc tcggagagcg caccgcccga
    12241 agggtggccg ttaggccagt ttctcgaaga gaaaccggta agtgcgccct cccctacaaa
    12301 gtagggtcgg gattgccgcc gctgtgcctc catgatagcc tacgagacag cacattaaca
    12361 atggggtgtc aagatggtta aggggagcaa caaggcggcg gatcggctgg ccaagctcga
    12421 agaacaacga gcgcgaatca atgccgaaat tcagcgggtg cgggcaaggg aacagcagca
    12481 agagcgcaag aacgaaacaa ggcgcaaggt gctggtgggg gccatgattt tggccaaggt
    12541 gaacagcagc gagtggccgg aggatcggct catggcggca atggatgcgt accttgaacg
    12601 cgaccacgac cgcgccttgt tcggtctgcc gccacgccag aaggatgagc cgggctgaat
    12661 gatcgaccga gacaggccct gcgg
//
