LOCUS       sequence-450932-       10753 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty lentivector to overexpress gene of interest.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10753)
  AUTHORS   Goodis CC, Eberly C, Chan AM, Kim M, Lowe BD, Civin CI, Fletcher S
  TITLE     The polypharmacy combination of the BCL-2 inhibitor venetoclax (VEN)
            and the FLT3 inhibitor gilteritinib (GIL) is more active in acute 
            myeloid leukemia cells than novel polypharmacologic BCL-2/FLT3 
            VEN-GIL hybrid single-molecule inhibitors.
  JOURNAL   Eur J Med Chem. 2024 Dec 22;285:117190. doi: 
            10.1016/j.ejmech.2024.117190.
  PUBMED    39813774
REFERENCE   2  (bases 1 to 10753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eur J Med 
            Chem."; date: "2024-12-22"; volume: "285:117190. doi"; pages: " 
            10.1016/j.ejmech.2024.117190"
COMMENT     Sequence Label: pMJK2243
FEATURES             Location/Qualifiers
     source          1..10753
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(131..199,200..991)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(349..368)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1162..1750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1651..1670
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1904..1921
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2038..2059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2074..2079,2080..2097,2098..2104)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2112..2128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2117..2139
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2136..2152
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2136..2152
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2171..2191
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2173..2191
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        2292..2671
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2672..2875
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2826..2846
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2890..3070
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3117..3242
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3735..3968
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4153..4197
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4490..4607
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     polyA_signal    4654..4878
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(4888..5487)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4971..4991)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     5468..5487
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(5488..5541)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(5611..6273)
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     promoter        complement(6304..7515)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(6448..6467)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     primer_bind     7526..7549
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     promoter        7598..8776
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7829..8767
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8724..8744
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     complement(8887..8907)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9305..9316)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9490..9723
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9795..9916
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9832..9851)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9886..9905
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9956..10091
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10111..10130)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10112..10130)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10140..10157)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10140..10156)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10149..10171)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10298..10726
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10385..10404)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10595..10616
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
       61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
      421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
      481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
      541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
      601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
      661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
      721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
      781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
      841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
      901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
      961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1561 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     2161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttggcc
     2221 attgcatacg ttgtatccat atcataatat gtacatttat attggctcat gtccaacatt
     2281 accgccatgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
     2341 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     2401 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     2461 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     2521 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     2581 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     2641 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
     2701 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     2761 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
     2821 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt
     2881 agtgaaccgg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     2941 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     3001 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     3061 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
     3121 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     3181 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     3241 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     3301 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     3361 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     3421 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     3481 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     3541 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct
     3601 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     3661 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     3721 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     3781 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     3841 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     3901 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     3961 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     4021 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     4081 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     4141 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     4201 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     4261 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     4321 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
     4381 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
     4441 ggatctcgac ggtatcgatc tcgacacaaa tggcagtatt catccacaat tttaaaagaa
     4501 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     4561 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
     4621 gggacagcag agatccagtt tgggtcgacg cgtccataga gcccaccgca tccccagcat
     4681 gcctgctatt gtcttcccaa tcctccccct tgctgtcctg ccccacccca ccccccagaa
     4741 tagaatgaca cctactcaga caatgcgatg caatttcctc attttattag gaaaggacag
     4801 tgggagtggc accttccagg gtcaaggaag gcacggggga ggggcaaaca acagatggct
     4861 ggcaactaga aggcacagtc gctcgagtca ggcaccgggc ttgcgggtca tgcaccaggt
     4921 gcgcggtcct tcgggcacct cgacgtcggc ggtgacggtg aagccgagcc gctcgtagaa
     4981 ggggaggttg cggggcgcgg aggtctccag gaaggcgggc accccggcgc gctcggccgc
     5041 ctccactccg gggagcacga cggcgctgcc cagacccttg ccctggtggt cgggcgagac
     5101 gccgacggtg gccaggaacc acgcgggctc cttgggccgg tgcggcgcca ggaggccttc
     5161 catctgttgc tgcgcggcca gccgggaacc gctcaactcg gccatgcgcg ggccgatctc
     5221 ggcgaacacc gcccccgctt cgacgctctc cggcgtggtc cagaccgcca ccgcggcgcc
     5281 gtcgtccgcg acccacacct tgccgatgtc gagcccgacg cgcgtgagga agagttcttg
     5341 cagctcggtg acccgctcga tgtggcggtc cggatcgacg gtgtggcgcg tggcggggta
     5401 gtcggcgaac gcggcggcga gggtgcgtac ggccctgggg acgtcgtcgc gggtggcgag
     5461 gcgcaccgtg ggcttgtact cggtcatagg gccgggattc tcctccacgt caccgcatgt
     5521 tagaagactt cctctgccct cgcgagatcc ggtggagccg ggtccggcgg tgccgtccac
     5581 ggcagaattg gacgactgag cgcgggatct ggcgaaggcg atgggggtct tgaaggcgtg
     5641 ctggtactcc acgatgccca gctcggtgtt gctgtgcagc tcctccacgc ggcggaaggc
     5701 gaacatgggg cccccgttct gcaggatgct ggggtggatg gcgctcttga agtgcatgtg
     5761 gctgtccacc acgaagctgt agtagccgcc gtcgcgcagg ctgaaggtgc gggcgaagct
     5821 gcccaccagc acgttatcgc ccatggggtg caggtgctcc acggtggcgt tgctgcggat
     5881 gatcttgtcg gtgaagatca cgctgtcctc ggggaagccg gtgcccacca ccttgaagtc
     5941 gccgatcacg cggccggcct cgtagcggta gctgaagctc acgtgcagca cgccgccgtc
     6001 ctcgtacttc tcgatgcggg tgttggtgta gccgccgttg ttgatggcgt gcaggaaggg
     6061 gttctcgtag ccgctggggt aggtgccgaa gtggtagaag ccgtagccca tcacgtggct
     6121 cagcaggtag gggctgaagg tcagggcgcc tttggtgctc ttcatcttgt tggtcatgcg
     6181 gccctgcttg ggggtgccct ctccgccgcc caccagctcg aactccacgc cgttcagggt
     6241 gccggtgatg cggcactcga tctccatggc gggcaggccg ctctcgtcgc tctccattga
     6301 tcagtctaac aaaaaagcca aaaacggcca gaatttagcg gacaatttac tagtctaaca
     6361 ctgaaaatta catattgacc caaatgatta catttcaaaa ggtgcctaaa aaacttcaca
     6421 aaacacactc gccaaccccg agcgcatagt tcaaaaccgg agcttcagct acttaagaag
     6481 ataggtacat aaaaccgacc aaagaaactg acgcctcact tatccctccc ctcaccagag
     6541 gtccggcgcc tgtcgattca ggagagccta ccctaggccc gaaccctgcg tcctgcgacg
     6601 gagaaaagcc taccgcacac ctaccggcag gtggccccac cctgcattat aagccaacag
     6661 aacgggtgac gtcacgacac gacgagggcg cgcgctccca aaggtacggg tgcactgccc
     6721 aacggcaccg ccataactgc cgcccccgca acagacgaca aaccgagttc tccagtcagt
     6781 gacaaacttc acgtcagggt ccccagatgg tgccccagcc catctcaccc gaataagagc
     6841 tttcccgcat tagcgaaggc ctcaagacct tgggttcttg ccgcccacca tgccccccac
     6901 cttgtttcaa cgacctcaca gcccgcctca caagcgtctt ccattcaaga ctcgggaaca
     6961 gccgccattt tgctgcgctc cccccaaccc ccagttcagg gcaaccttgc tcgcggaccc
     7021 agactacagc ccttggcggt ctctccacac gcttccgtcc caccgagcgg cccggcggcc
     7081 acgaaagccc cggccagccc agcagcccgc tactcaccaa gtgacgatca cagcgatcca
     7141 caaacaagaa ccgcgaccca aatcccggct gcgacggaac tagctgtgcc acacccggcg
     7201 cgtccttata taatcatcgg cgttcaccgc cccacggaga tccctccgca gaatcgccga
     7261 gaagggacta cttttcctcg cctgttccgc tctctggaaa gaaaaccagt gccctagagt
     7321 cacccaagtc ccgtcctaaa atgtccttct gctgatactg gggttctaag gccgagtctt
     7381 atgagcagcg ggccgctgtc ctgagcgtcc gggcggaagg atcaggacgc tcgctgcgcc
     7441 cttcgtctga cgtggcagcg ctcgccgtga ggaggggggc gcccgcggga ggcgccaaaa
     7501 cccggcgcat gcatcgccaa gtgcccagcg gggctgctaa agcgcatgct ccagactgcc
     7561 ttgggaaaag cgcctcccct acccggtaga agctagcggc tccggtgccc gtcagtgggc
     7621 agagcgcaca tcgcccacag tccccgagaa gttgtgggga ggggtcggca attgaaccgg
     7681 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
     7741 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
     7801 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
     7861 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
     7921 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
     7981 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
     8041 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
     8101 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
     8161 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
     8221 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
     8281 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
     8341 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
     8401 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
     8461 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     8521 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgacct tttggagtac
     8581 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     8641 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     8701 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     8761 atttcaggtg tcgtgatcta gagctagcga attcgaattt aaatcggatc cttaattaag
     8821 gtacctgata tcaactcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     8881 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     8941 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     9001 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     9061 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     9121 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     9181 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     9241 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     9301 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     9361 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     9421 gcactttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     9481 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     9541 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     9601 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     9661 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     9721 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     9781 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     9841 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     9901 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     9961 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
    10021 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
    10081 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
    10141 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
    10201 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
    10261 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
    10321 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
    10381 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
    10441 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
    10501 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
    10561 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
    10621 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
    10681 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
    10741 acgcttacaa ttt
//
