LOCUS       sequence-450958-       11373 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual fluorescent reporter for histone H2B and for APC/C activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11373)
  AUTHORS   Kim S, Armand J, Safonov A, Zhang M, Soni RK, Schwartz G, McGuinness
            JE, Hibshoosh H, Razavi P, Kim M, Chandarlapaty S, Yang HW
  TITLE     Sequential activation of E2F via Rb degradation and c-Myc drives 
            resistance to CDK4/6 inhibitors in breast cancer.
  JOURNAL   Cell Rep. 2023 Nov 28;42(11):113198. doi: 
            10.1016/j.celrep.2023.113198. Epub 2023 Oct 21.
  PUBMED    37865915
REFERENCE   2  (bases 1 to 11373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2023-11-28"; volume: "42(11):113198. doi"; pages: " 
            10.1016/j.celrep.2023.113198. Epub 2023 Oct 21"
COMMENT     Sequence Label: pLV-H2B-iRFP670-p2a-mCerulean-Geminin
            (1–110)-...
FEATURES             Location/Qualifiers
     source          1..11373
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    680..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1302..1535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1720..1764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2027..2142
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2220..2431
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2432..3370
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3327..3347
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3392..3769
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             3776..4705
                     /codon_start=1
                     /product="near-infrared fluorescent protein with an 
                     emission peak at 670 nm, engineered from a bacterial 
                     phytochrome (Shcherbakova and Verkhusha, 2013)"
                     /label=iRFP670
                     /note="derived from Rhodopseudomonas palustris RpBphP6"
                     /translation="ARKVDLTSCDREPIHIPGSIQPCGCLLACDAQAVRITRITENAGA
                     FFGRETPRVGELLADYFGETEAHALRNALAQSSDPKRPALIFGWRDGLTGRTFDISLHR
                     HDGTSIIEFEPAAAEQADNPLRLTRQIIARTKELKSLEEMAARVPRYLQAMLGYHRVML
                     YRFADDGSGMVIGEAKRSDLESFLGQHFPASLVPQQARLLYLKNAIRVVSDSRGISSRI
                     VPEHDASGAALDLSFAHLRSISPCHLEFLRNMGVSASMSLSIIIDGTLWGLIICHHYEP
                     RAVPMAQRVAAEMFADFLSLHFTAAHHQR"
     CDS             4724..4780
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(4781..4783,4784..5494)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4823..4844)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5084..5103)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5431..5452
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5525..5854
                     /codon_start=1
                     /product="degron consisting of residues 1-110 of human 
                     Geminin (Zielke and Edgar, 2015)"
                     /label=Gem1 (1-110)
                     /translation="MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELS
                     AGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAE
                     KRRKAL"
     misc_feature    5971..6520
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6115..6132)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6342..6361
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6523..7317
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6577..6596)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7187..7206
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    7341..7929
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7394..7414)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7812..7823)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8136..8769
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8897..8913)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8897..8913)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8910..8932)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8921..8937
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8945..8951,8952..8969,8970..8975))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8990..9011
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9128..9145)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9299..9887)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9379..9398)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10058..10849,10850..10918))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10681..10700
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10919..11023)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    11071..11205
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11108..11127)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11162..11181
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(11373,1..633)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
       61 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
      121 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
      181 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
      241 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
      301 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
      361 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
      421 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
      481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg
     2041 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2101 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2161 cagcagagat ccagtttatc gatgaggccc tttcgtcttc actcgaggtg cccgtcagtg
     2221 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
     2281 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
     2341 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
     2401 ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc
     2461 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta
     2521 cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc
     2581 ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg
     2641 cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat
     2701 ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc
     2761 gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg
     2821 tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg
     2881 acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat
     2941 cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg
     3001 gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg
     3061 ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg
     3121 tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag
     3181 tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg
     3241 ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct
     3301 ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct
     3361 tccatttcag gtgtcgtgag gatccgccac catgccagag ccagcgaagt ctgctcccgc
     3421 cccgaaaaag ggctccaaga aggcggtgac taaggcgcag aagaaaggcg gcaagaagcg
     3481 caagcgcagc cgcaaggaga gctattccat ctatgtgtac aaggttctga agcaggtcca
     3541 ccctgacacc ggcatttcgt ccaaggccat gggcatcatg aattcgtttg tgaacgacat
     3601 tttcgagcgc atcgcaggtg aggcttcccg cctggcgcat tacaacaagc gctcgaccat
     3661 cacctccagg gagatccaga cggccgtgcg cctgctgctg cctggggagt tggccaagca
     3721 cgccgtgtcc gagggtacta aggccatcac caagtacacc agcgctaagg atccagcgcg
     3781 taaggtcgat ctcacctcct gcgatcgcga gccgatccac atccccggca gcattcagcc
     3841 gtgcggctgc ctgctagcct gcgacgcgca ggcggtgcgg atcacgcgca ttacggaaaa
     3901 tgccggcgcg ttctttggac gcgaaactcc gcgggtcggt gagctactcg ccgattactt
     3961 cggcgagacc gaagcccatg cgctgcgcaa cgcactggcg cagtcctccg atccaaagcg
     4021 accggcgctg atcttcggtt ggcgcgacgg cctgaccggc cgcaccttcg acatctcact
     4081 gcatcgccat gacggtacat cgatcatcga gttcgagcct gcggcggccg aacaggccga
     4141 caatccgctg cggctgacgc ggcagatcat cgcgcgcacc aaagaactga agtcgctcga
     4201 agagatggcc gcacgggtgc cgcgctatct gcaggcgatg ctcggctatc accgcgtgat
     4261 gttgtaccgc ttcgcggacg acggctccgg gatggtgatc ggcgaggcga agcgcagcga
     4321 cctcgagagc tttctcggtc agcactttcc ggcgtcgctg gtcccgcagc aggcgcggct
     4381 actgtacttg aagaacgcga tccgcgtggt ctcggattcg cgcggcatca gcagccggat
     4441 cgtgcccgag cacgacgcct ccggcgccgc gctcgatctg tcgttcgcgc acctgcgcag
     4501 catctcgccc tgccatctcg aatttctgcg gaacatgggc gtcagcgcct cgatgtcgct
     4561 gtcgatcatc attgacggca cgctatgggg attgatcatc tgtcatcatt acgagccgcg
     4621 tgccgtgccg atggcgcagc gcgtcgcggc cgaaatgttc gccgacttct tatcgctgca
     4681 cttcaccgcc gcccaccacc aacgcctgta caagggttcc ggagccacga acttctctct
     4741 gttaaagcaa gcaggagacg tggaagaaaa tcccggtcct gtgagcaagg gcgaggagct
     4801 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     4861 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     4921 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctgggg
     4981 cgtgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     5041 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     5101 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     5161 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca acgccatcag
     5221 cgacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat
     5281 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     5341 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct
     5401 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     5461 cgggatcact ctcggcatgg acgagctgta caagggatat ccatcacact ggcggccgct
     5521 cgagatgaat cccagtatga agcagaaaca agaagaaatc aaagagaata taaagaatag
     5581 ttctgtccca agaagaactc tgaagatgat tcagccttct gcatctggat ctcttgttgg
     5641 aagagaaaat gagctgtccg caggcttgtc caaaaggaaa catcggaatg accacttaac
     5701 atctacaact tccagccctg gggttattgt cccagaatct agtgaaaata aaaatcttgg
     5761 aggagtcacc caggagtcat ttgatcttat gattaaagaa aatccatcct ctcagtattg
     5821 gaaggaagtg gcagaaaaac ggagaaaggc gctgtaagaa ttcctcgagg gcggccgctc
     5881 tagagtcgac gggccgcggt aacaattgtt aactaactta agctagcaac ggtttccctc
     5941 tagcgggatc aattccgccc ccccccccta acgttactgg ccgaagccgc ttggaataag
     6001 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
     6061 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
     6121 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
     6181 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
     6241 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
     6301 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
     6361 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
     6421 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
     6481 accacgggga cgtggttttc ctttgaaaaa cacgataata ccatgattga acaagatgga
     6541 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     6601 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     6661 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg
     6721 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     6781 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     6841 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     6901 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     6961 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     7021 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg
     7081 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     7141 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     7201 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     7261 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgaccc
     7321 gggcggggcg cgtctggaac aatcaacctc tggattacaa aatttgtgaa agattgactg
     7381 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     7441 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     7501 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     7561 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     7621 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     7681 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
     7741 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     7801 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     7861 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     7921 cctccccgcc tggaattaat tctgcagtcg agacctagaa aaacatggag caatcacaag
     7981 tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga
     8041 ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt
     8101 agatcttagc cactttttaa aagaaaagag gggactggaa gggctaattc actcccaacg
     8161 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca
     8221 gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct
     8281 agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt
     8341 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt
     8401 tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa
     8461 ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg
     8521 cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg
     8581 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     8641 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     8701 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     8761 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     8821 gaatatcaga gagtgagagg ccttgacatt gctagcgttt accgtcgacc tctagctaga
     8881 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     8941 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     9001 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     9061 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     9121 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     9181 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     9241 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     9301 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     9361 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     9421 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     9481 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     9541 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     9601 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     9661 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     9721 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     9781 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     9841 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     9901 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     9961 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
    10021 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
    10081 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
    10141 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
    10201 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
    10261 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
    10321 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
    10381 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
    10441 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
    10501 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    10561 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    10621 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    10681 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    10741 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    10801 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    10861 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    10921 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    10981 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    11041 tgccacctga cgtcgacgga tcgggagatc aacttgttta ttgcagctta taatggttac
    11101 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
    11161 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa
    11221 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg
    11281 tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt
    11341 atttgttaaa tatgtactac aaacttagta gtt
//
