LOCUS       sequence-45096-e        2477 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Seeliger JC, Topp S, Sogi KM, Previti ML, Gallivan JP, Bertozzi CR
  TITLE     A riboswitch-based inducible gene expression system for 
            mycobacteria.
  JOURNAL   PLoS One. 2012;7(1):e29266. Epub 2012 Jan 18.
  PUBMED    22279533
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(1)"; pages: "e29266. Epub 2012 Jan 18"
COMMENT     Sequence Label: pRiboS BsaHind
FEATURES             Location/Qualifiers
     source          1..2477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      376..422
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(572..591)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             583..1398
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(656..675)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1729..2317
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2218..2237
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcggccgcgg taccagatct ttaaatctag aggtgaccac aacgcgcccg ctttgatcgg
       61 ggacgtctgc ggccgaccat ttacgggtct tgttgtcgtt ggcggtcatg ggccgaacat
      121 actcacccgg atcggagggc cgaggacaag gtcgaacgag gggcatgacc cggtgcgggg
      181 cttcttgcac tcggcatagg cgagtgctaa gaataacgtt ggcactcgcg accgggatac
      241 gactcactat aggtaccggt gataccagca tcgtcttgat gcccttggca gcaccctgct
      301 aaggaggcaa caagatggag accaagctta tcgatgtcga cgtagttaac tagcgtacga
      361 tcgactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
      421 atgccatcat ggccgcggtg atcagctagc cacctgacgt cggggggggg ggaaagccac
      481 gttgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca
      541 ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg
      601 gaaacgtctt gctcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat
      661 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag
      721 ccccatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca
      781 gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat
      841 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg gaaaacagca
      901 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg
      961 ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta
     1021 tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt
     1081 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taatcttttg
     1141 ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt
     1201 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac
     1261 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg
     1321 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg
     1381 ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg
     1441 ctgacttgac gggacggcgg ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc
     1501 agatcacgca tcttcccgac aacgcagacc gttccgtggc aaagcaaaag ttcaaaatca
     1561 ccaactggtc cacctacaac aaagctctca ccaaccgtgg ctccctcact ttctggctgg
     1621 atgatggggc gattcaggcc tggtatgagt cagcaacacc ttcttcacga ggcagacctc
     1681 actagttcca tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     1741 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     1801 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     1861 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     1921 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     1981 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     2041 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     2101 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgagggga gaaaggcgga
     2161 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     2221 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     2281 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     2341 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     2401 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     2461 gaccgagcgc aacgcgt
//
