LOCUS       sequence-450963-       11329 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses all necessary components of Type I-C CRISPR-Cas system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11329)
  AUTHORS   Lammens E-M, Volke DC, Schroven K, Voet M, Kerremans A, Lavigne R, 
            Hendrix H
  TITLE     A SEVA-based, CRISPR-Cas3-assisted genome engineering approach for 
            Pseudomonas with efficient vector curing.
  JOURNAL   Microbiol Spectr. 2023 Nov 17:e0270723. doi: 
            10.1128/spectrum.02707-23.
  PUBMED    37975669
REFERENCE   2  (bases 1 to 11329)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microbiol 
            Spectr. 2023 Nov 17:e0270723. doi: 10.1128/spectrum.02707-23."
COMMENT     Sequence Label: pCas3-Apr
FEATURES             Location/Qualifiers
     source          1..11329
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      875..884
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(2051..2062)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     oriT            4863..4971
                     /note="incP origin of transfer"
     rep_origin      complement(5041..5629)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5121..5140)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      5666..5759
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             5966..6766
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     promoter        complement(join(6981..6986,6987..7003,7004..7009))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      7050..7401
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from 
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     CDS             7415..8248
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pRO1600 from Pseudomonas aeruginosa "
                     /label=pRO1600 Rep
                     /translation="MASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPARRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     CDS             complement(8249..9097)
                     /codon_start=1
                     /gene="E. coli rhaR"
                     /product="transcriptional activator of rhaR and rhaS"
                     /label=rhaR
                     /note="needed for rhamnose-dependent activation of the rhaB
                     promoter"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVW
                     RGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAI
                     PGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHR
                     YTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTIN
                     QYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQK
                     D"
     CDS             complement(9171..10007)
                     /codon_start=1
                     /gene="E. coli rhaS"
                     /product="positive regulator of the rhaB promoter
                     "
                     /label=rhaS
                     /note="activated by L-rhamnose"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGT
                     GIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQ
                     ELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQ
                     ENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNR
                     LRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ"
     promoter        10153..10271
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring 
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
ORIGIN
        1 gagcatcaca gcgccttcgt tgatgaccgc cggcagagcc tggaggccaa gaagaaactg
       61 aacctagcga tggagaactg ggacgcgcct atcgtggtga ccactgcagt gcagttcttc
      121 gaaagcctgt ttgccgaccg tccagcccag tgccgcaagc tacacaacat cgccggcagc
      181 gtggtgattc ttgacgaggc acagacccta ccgctcaagc tgttgcggcc ctgcgttgcc
      241 gcccttgatg aactggcgct caactaccgt tgtagcccag ttctctgtac tgccacgcag
      301 ccagcgcttc aatcgccgga tttcatcggt gggctgcagg acgtacgtga gctggcgccc
      361 gagccgcagc ggctgttccg ggagttggtg cgggtacgaa tacggacatt gggcccgctc
      421 gaagatgcgg ccttgactga gcagatcgcc aggcgtgaac aagtgctgtg catcgtcaac
      481 aatcgacgcc aggcccgtgc gctctatgag tcgcttgccg agttgcccgg tgcccgccat
      541 ctcaccaccc tgatgtgcgc caagcaccgt agcagcgtgc tggccgaggt gcgccagatg
      601 ctcaaaaagg gggagccctg tcgcctggtg gccacctcgc tgatcgaggc cggtgtggat
      661 gtggattttc ccgtggtact gcgtgccgag gctggattgg attccatcgc ccaggccgcg
      721 ggacgctgca atcgcgaagg caagcggccg ctggccgaaa gcgaggtgct ggtgttcgcc
      781 gcggccaatt ctgactgggc gccacccgag gaactcaagc agttcgccca ggccgcccgc
      841 gaagtgatgc gcctgcaccc ggatgattgc ctgtccatgg cggccatcga gcggtatttt
      901 cgcatactgt actggcagaa gggcgcggag gagttggatg cgggtaacct gctcggcctg
      961 attgagagag gccggctcga tggcctgccc tacgagactt tggccaccaa gttccgcatg
     1021 atcgacagcc ttcaactgcc ggtgatcatc ccatttgatg acgaggccag agcagccctg
     1081 cgcgagctgg agttcgccga cggctgcgcc gccatcgccc gtcgcctgca gccatatctg
     1141 gtgcagatgc cacgcaaggg ttatcaggca ttgcgggaag ccggtgcgat ccaggcggcg
     1201 gcaggtacgc gttatggtga gcagtttatg gcgttggtca accctgatct gtatcaccac
     1261 caattcgggt tgcactggga taatccggcc tttgtcagca gcgagcggct atgttggtag
     1321 tcgggacgcg caacagcggc ctggcctgga tgatgtgaaa gggagggccg atggcctacg
     1381 gaattcgctt aatggtctgg ggcgagcgtg cctgcttcac ccgcccggaa atgaaggtgg
     1441 aacgcgtctc ttacgatgcg atcacgccgt ccgccgcgcg cggcattctc gaggctatcc
     1501 actggaagcc ggcgattcgc tgggtggtgg atcgcattca agtgcttaag ccgatccgct
     1561 tcgaatccat ccggcgcaac gaggtcggcg gcaagctgtc cgctgtcagc gtcggtaagg
     1621 caatgaaggc cgggcgtact aatggtctgg tgaatctggt cgaggaggat cgccagcagc
     1681 gcgcgactac tctgctgcgc gatgtctcct atgtcatcga ggcgcatttc gagatgactg
     1741 acagggctgg cgccgacgat acggtgggca agcatctgga tatcttcaac cgtcgcgcac
     1801 ggaaggggca gtgcttccat acaccctgcc taggcgtgcg cgagtttccg gccagttttc
     1861 ggttgctgga agagggcagt gccgagcctg aagtcgatgc ctttctgcgc ggcgagcgtg
     1921 atctgggctg gatgctgcat gacattgact tcgccgatgg catgaccccg cacttcttcc
     1981 gtgccctgat gcgcgatggg ctgatcgagg tgccggcctt cagggcggca gaggacaagg
     2041 catgatcctt tcggccctca atgactatta tcagcgactg ctggagcggg gtgaagcgaa
     2101 tatctcaccc ttcggctaca gccaagaaaa gatcagttac gccctgctgc tgtccgcaca
     2161 aggagagttg ctggacgtgc aggacattcg cttgctctct ggcaagaagc ctcaacccag
     2221 gcttatgagt gtgccgcagc cggagaagcg cacctcgggc atcaagtcca acgtactgtg
     2281 ggacaagacc agctatgtgc tgggtgttag tgccaagggc ggagagcgta ctcagcagga
     2341 gcacgagtcc ttcaagacgc tgcaccggca gatcttggtt ggggaaggcg accccggtct
     2401 gcaggccttg ctccagttcc tcgactgttg gcagccggag cagttcaagc ccccgctgtt
     2461 cagcgaagca atgctcgaca gcaacttagt gttccgccta gacggccaac aacgctatct
     2521 gcacgagact ccggcggccc tggcgttgcg tacccggctg ttggccgacg gcgacagccg
     2581 cgaggggctg tgcctagtct gcggccaacg tcagccgttg gcgcgcctgc atccagcggt
     2641 caagggcgtc aatggtgccc agagttcggg ggcttccatc gtctccttca acctcgacgc
     2701 tttttcctcc tacggcaaga gccaggggga aaatgctccg gtgtccgaac aggccgcctt
     2761 tgcctacacc acggtgctca accatttgtt gcgtcgcgac gagcacaacc gccagcgcct
     2821 gcagattggc gacgcgagtg tggtgttctg ggcgcaggcg gatactcctg ctcaggtggc
     2881 cgccgccgag tcgaccttct ggaacctgct ggagccaccc gcagatgatg gtcaggaagc
     2941 ggaaaagctg cgcggcgtgc tggatgctgt ggccacgggg cggcccttgc atgagctcga
     3001 ctcgctaatg gaggaaggca cccgcatttt tgtgttaggg ctggcgccca atacctcgcg
     3061 actgtccatt cggttctggg cagtcgatag ccttgcggta ttcacccagc atctggccga
     3121 gcatttccgg gatatgcacc ttgagcctct gccctggaag acggagccgg ccatctggcg
     3181 cttgctctat gctaccgcgc ccagtcgtga cggcagagcc aagaccgaag acttactccc
     3241 acaactggcc ggtgaaatga cccgcgccat cctgaccggc agccgctatc cgcgcagttt
     3301 gctagccaac ctgatcatgc gcatgcgtgc cgacggcgac gtctctggca tacgcgtcgc
     3361 gctgtgcaag gccgtgctcg ctcgcgaggc acgcctgagc ggcaaaattc accaagagga
     3421 gctacctatg agtctcgaca aggacgccag caaccccggc tatcgcttgg ggaggctgtt
     3481 cgccgtgttg gaaggcgccc agcgcgcagc cctgggcgac agggtcaatg ccactatccg
     3541 tgaccgctac tacggtgccg cgtccagcac gccagccacg gttttcccga tactgctgcg
     3601 caacacacaa aaccacttgg ccaagctgcg caaggagaag cccggactag cagtgaacct
     3661 agagcgcgat ataggcgaaa tcattgacgg tatgcagagc caattcccgc gttgcctgcg
     3721 cctggaggac cagggacgct ttgctattgg ttactaccaa caggcccagg cccgtttcaa
     3781 ccgtggcccc gattccgtcg agtaaggagc agaagaatga ccgccatctc caaccgctac
     3841 gagttcgttt acctctttga tgtcagcaat ggcaatccca atggcgaccc ggatgctggc
     3901 aacatgccgc gtctcgatcc ggaaaccaac caggggttgg tcactgacgt ttgcctcaag
     3961 cgcaagatcc gcaactacgt cagcctggag caggaaagtg cccccggcta tgccatctat
     4021 atgcaggaaa aatccgtgct gaataaccag cacaaacagg cctacgaggc gctcggtatc
     4081 gagtcagagg caaagaaact gcccaaggac gaagccaagg cgcgcgaact gacctcttgg
     4141 atgtgcaaga acttcttcga tgtgcgtgct ttcggggcgg tgatgaccac cgagattaat
     4201 gccggccagg tgcgtggacc gatccaactg gcattcgcca cgtctatcga cccggtattg
     4261 cctatggagg tatccatcac ccgcatggcg gtgactaacg aaaaggattt ggagaaggaa
     4321 cgcaccatgg gacgcaagca catcgtgcct tacggcttgt accgcgccca tggtttcatc
     4381 tctgccaagt tggccgagcg aaccggcttt tccgacgacg acttggaact gctatggcgc
     4441 gctttggcca atatgttcga acacgaccgc tcggcggcac gtggcgagat ggcagcgcgc
     4501 aagttgatcg tcttcaagca tgagcatgcc atgggcaatg cacccgccca tgtgctgttc
     4561 ggcagcgtta aggtcgagcg agtcgagggg gacgcagtta caccagcacg cggtttccag
     4621 gattaccgtg tcagcatcga tgcggaagct ctgcctcagg gcgtgagcgt gcgcgagtac
     4681 ctctagtttc aggtatcgat accgtcgacg gggattcctt aaggtatact ttccgctgca
     4741 taaccctgct tcggggtcat tatagcgatt ttttcggtat atccatcctt tttcgcacga
     4801 tatacaggat tttgccaaag ggttcgtgta gactttcctt ggtgtatcca acggcgtcag
     4861 ccgggcagga taggtgaagt aggcccaccc gcgagcgggt gttccttctt cactgtccct
     4921 tattcgcacc tggcggtgct caacgggaat cctgctctgc gaggctggcc gataagctag
     4981 cttgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     5041 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     5101 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaggct ccctcgtgcg
     5161 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     5221 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     5281 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     5341 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     5401 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     5461 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     5521 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     5581 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     5641 gatcttttct acggggtctg acgctactcc tgttgataga tccagtaatg acctcagaac
     5701 tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt ggtgagaatc
     5761 caggggtccc caataattac gatttaaatc tcacggtaac tgatgccgta tttgcagtac
     5821 cagcgtacgg cccacagaat gatgtcacgc tgaaaatgcc ggcctttgaa tgggttcatg
     5881 tgcagctcca tcagcaaaag gggatgataa gtttatcacc accgactatt tgcaacagtg
     5941 ccgttgatcg tgctatgatc gagtgatgtc atcagcggtg gagtgcaatg tcgtgcaata
     6001 cgaatggcga aaagccgagc tgatcggtca gcttctcaac cttggggtta cccccggcgg
     6061 tgtgctgctg gtccacagct ccttccgtag cgtccggccc ctcgaagatg ggccacttgg
     6121 actgatcgag gccctgcgtg ctgcgctggg tccgggaggg acgctcgtga tgccctcgtg
     6181 gtcaggtctg gacgacgagc cgttcgatcc tgccacgtcg cccgttacac cggaccttgg
     6241 agttgtctct gacacattct ggcgcctgcc aaatgtaaag cgcagcgccc atccatttgc
     6301 ctttgcggca gcggggccac aggcagagca gatcatctct gatccattgc ccctgccacc
     6361 tcactcgcct gcaagcccgg tcgcccgtgt ccatgaactc gatgggcagg tacttctcct
     6421 cggcgtggga cacgatgcca acacgacgct gcatcttgcc gagttgatgg caaaggttcc
     6481 ctatggggtg ccgagacact gcaccattct tcaggatggc aagttggttc gcgtcgatta
     6541 tctcgagaat gaccactgct gtgagcgctt tgccttggcg gacaggtggc tcaaggagaa
     6601 gagccttcag aaggaaggtc cagtcggtca tgcctttgct cggttgatcc gctcccgcga
     6661 cattgtggcg acagccctgg gtcaactggg ccgagatccg ttgatcttcc tgcatccgcc
     6721 agaggcggga tgcgaagaat gcgatgccgc tcgccagtcg attggctaag accgcggtcc
     6781 gcgcgttgtc gatgccgctc gccagtcgat tggctaagac cgcggtccgc gcgttgtcct
     6841 ttgatgccgc tcgccagtcg attggctaag accgcggtcc gcgcgttgtc cttttccgct
     6901 gcataaccct gcttcggggt cattatagcg attttttcgg tatatccatc ctgcgtgagc
     6961 gcatacgcta cttgcattac agtttacgaa ccgaacaggc ttatgtcaat tcgcgccgct
     7021 ggtgccgctg gttggacgcc aagggtgaat ccgcctcgat accctgatta ctcgcttcct
     7081 gcgccctctc aggcggcgat aggggactgg taaaacgggg attgcccaga cgcctccccc
     7141 gccccttcag gggcacaaat gcggccccaa cggggccacg tagtggtgcg ttttttgcgt
     7201 ttccaccctt ttcttccttt tcccttttaa accttttagg acgtctacag gccacgtaat
     7261 ccgtggcctg tagagtttaa aaagggacgg atttgttgcc attaagggac ggatttgttg
     7321 ttaagaaggg acggatttgt tgttgtaaag ggacggattt gttgtattgt gggacgcaga
     7381 tacagtgtcc ccttatacac aaggaatgtc gaacgtggcc tcacccccaa tggtttacaa
     7441 aagcaatgcc ctggtcgagg ccgcgtatcg cctcagtgtt caggaacagc ggatcgttct
     7501 ggcctgtatt agccaggtga agaggagcga gcctgtcacc gatgaagtga tgtattcagt
     7561 gacggcggag gacatagcga cgatggcggg tgtccctatc gaatcttcct acaaccagct
     7621 caaagaagcg gccctgcgcc tgaaacggcg ggaagtccgg ttaacccaag agcccaatgg
     7681 caaggggaaa agaccgagtg tgatgattac cggctgggtg caaacaatca tctaccggga
     7741 gggtgagggc cgtgtagaac tcaggttcac caaagacatg ctgccgtacc tgacggaact
     7801 caccaaacag ttcaccaaat acgccttggc tgacgtggcc aagatggaca gcacccacgc
     7861 gatcaggctt tacgagctgc tcatgcaatg ggacagcatc ggccagcgcg aaatagaaat
     7921 tgaccagctg cgaaagtggt ttcaactgga aggccggtat ccctcgatca aggacttcaa
     7981 gttgcgagtg cttgatccag ccgtgacgca gatcaacgag cacagcccgc tacaggtgga
     8041 gtgggcgcag cgaaagaccg ggcgcaaggt cacacatctg ttgttcagtt ttggaccgaa
     8101 gaagcccgcc aaggcggtgg gtaaggcccc agcgaggcgc aaggccggga agatttcaga
     8161 tgctgagatc gcgaaacagg ctcgccctgg tgagacatgg gaagcggccc gcgctcgact
     8221 aacccagatg ccgctggatc tggcctagtt aatctttctg cgaattgaga tgacgccact
     8281 ggctgggcgt catcccggtt tcccgggtaa acaccaccga aaaatagtta ctatcttcaa
     8341 agccacattc ggtcgaaata tcactgatta acaggcggct atgctggaga agatattgcg
     8401 catgacacac tctgacctgt cgcagatatt gattgatggt cattccagtc tgctggcgaa
     8461 attgctgacg caaaacgcgc tcactgcacg atgcctcatc acaaaattta tccagcgcaa
     8521 agggactttt caggctagcc gccagccggg taatcagctt atccagcaac gtttcgctgg
     8581 atgttggcgg caacgaatca ctggtgtaac gatggcgatt cagcaacatc accaactgcc
     8641 cgaacagcaa ctcagccatt tcgttagcaa acggcacatg ctgactactt tcatgctcaa
     8701 gctgaccgat aacctgccgc gcctgcgcca tccccatgct acctaagcgc cagtgtggtt
     8761 gccctgcgct ggcgttaaat cccggaatcg ccccctgcca gtcaagattc agcttcagac
     8821 gctccgggca ataaataata ttctgcaaaa ccagatcgtt aacggaagcg taggagtgtt
     8881 tatcgtcagc atgaatgtaa aagagatcgc cacgggtaat gcgataaggg cgatcgttga
     8941 gtacatgcag gccattaccg cgccagacaa tcaccagctc acaaaaatca tgtgtatgtt
     9001 cagcaaagac atcttgcgga taacggtcag ccacagcgac tgcctgctgg tcgctggcaa
     9061 aaaaatcatc tttgagaagt tttaactgat gcgccaccgt ggctacctcg gccagagaac
     9121 gaagttgatt attcgcaata tggcgtacaa atacgttgag aagattcgcg ttattgcaga
     9181 aagccatccc gtccctggcg aatatcacgc ggtgaccagt taaactctcg gcgaaaaagc
     9241 gtcgaaaagt ggttactgtc gctgaatcca cagcgatagg cgatgtcagt aacgctggcc
     9301 tcgctgtggc gtagcagatg tcgggctttc atcagtcgca ggcggttcag gtatcgctga
     9361 ggcgtcagtc ccgtttgctg cttaagctgc cgatgtagcg tacgcagtga aagagaaaat
     9421 tgatccgcca cggcatccca attcacctca tcggcaaaat ggtcctccag ccaggccaga
     9481 agcaagttga gacgtgatgc gctgttttcc aggttctcct gcaaactgct tttacgcagc
     9541 aagagcagta attgcataaa caagatctcg cgactggcgg tcgagggtaa atcattttcc
     9601 ccttcctgct gttccatctg tgcaaccagc tgtcgcacct gctgcaatac gctgtggtta
     9661 acgcgccagt gagacggata ctgcccatcc agctcttgtg gcagcaactg attcagcccg
     9721 gcgagaaact gaaatcgatc cggcgagcga tacagcacat tggtcagaca cagattatcg
     9781 gtatgttcat acagatgccg atcatgatcg cgtacgaaac agaccgtgcc accggtgatg
     9841 gtatagggct gcccattaaa cacatgaata cccgtgccat gttcgacaat cacaatttca
     9901 tgaaaatcat gatgatgttc aggaaaatcc gcctgcggga gccggggttc tatcgccacg
     9961 gacgcgttac cagacggaaa aaaatccaca ctatgtaata cggtcatact ggcctcctga
    10021 tgtcgtcaac acggcgaaat agtaatcacg aggtcaggtt cttaccttaa attttcgacg
    10081 gaaaaccacg taaaaaacgt cgatttttca agatacagcg tgaattttca ggaaatgcgg
    10141 tgagcatcac atcaccacaa ttcagcaaat tgtgaacatc atcacgttca tctttccctg
    10201 gttgccaatg gcccattttc ctgtcagtaa cgagaaggtc gcgaattcag gcgcttttta
    10261 gactggtcgt aatgaaattc aactaggtcg cgccccgcac gggcgcgtgg attgaaacga
    10321 gacctctctg gacaaaggtc tcgtcgcccg gcaaaaccgg gcgtggattg aaacctagtg
    10381 ctctgcagga attcctcgag aagggagcgc acctatggac gcggaggcta gcgatactca
    10441 cttttttgct cactccacct taaaggcaga tcgcagcgat tggcagcctc tggtcgagca
    10501 tctacaggct gttgcccgtt tggcaggaga gaaggctgcc ttcttcggcg gcggtgaatt
    10561 agctgctctt gctggtctgt tgcatgactt gggtaaatac actgacgagt ttcagcggcg
    10621 tattgcgggt gatgccatcc gtgtcgatca ctctactcgc ggggccatac tggcggtaga
    10681 acgctatggc gcgctaggtc aattgctagc ctacggcatc gctggccacc atgccgggtt
    10741 ggccaatggc cgcgaggctg gtgagcgaac tgccttggtc gaccgcctga aaggggttgg
    10801 gctgccacgg ttattggagg ggtggtgcgt ggaaatcgtg ctacccgagc gccttcaacc
    10861 accgccacta aaagcgcgcc tggaaagagg tttctttcag ttggcctttc ttggccggat
    10921 gctcttttcc tgcttggttg atgcggatta tctagatacc gaagccttct accaccgcgt
    10981 cgaaggacgg cgctcccttc gcgagcaagc gcggccgacc ttggccgagt tacgcgcagc
    11041 ccttgatcgg catctgactg agttcaaggg agatacgccg gtcaaccgcg ttcgcgggga
    11101 gatattggcc ggcgtgcgcg gcaaggcgag cgaacttccc gggctgtttt ctctcacagt
    11161 gcccacagga ggcggcaaga ccctggcctc tctggctttc gccctggatc acgctctagc
    11221 tcatgggctg cgccgggtga tctacgtgat tcccttcact agcatcgtcg agcagaacgc
    11281 tgcggtattc cgtcgtgcac tcggggcctt aggcgaagag gcggtgctg
//
