LOCUS       sequence-450965-        9041 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pharmacologically Selective Actuator Module, chimeric ligand-gated 
            ion channels that can be used to manipulate electrophysiological 
            activity..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9041)
  TITLE     Tonic dendritic GABA release by substantia nigra dopaminergic 
            neurons
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: EF1a-DIO-PSAML-141F,Y115F- GlyR-2A-FusionRed
FEATURES             Location/Qualifiers
     source          1..9041
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  132..297
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      481..935
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(568..587)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     777..798
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        961..1065
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1066..1134,1135..1926)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1284..1303)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2097..2685
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2586..2605
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  2990..3214
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(3281..3814)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3984..3989,3990..4006,4007..4012))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     repeat_region   4352..4492
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4363..4492
                     /label=AAV2 ITR
     promoter        4507..5697
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4742..5688
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5645..5665
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    5717..5750
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    5813..5846
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(5857..6555)
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
     CDS             complement(6556..6612)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(7934..7967)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(8038..8071)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    8104..8692
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8157..8177)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8575..8586)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8719..8736)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8725..8949
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   join(8978..9041,1..77)
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   join(8978..9041,1..66)
                     /label=AAV2 ITR
ORIGIN
        1 gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc
       61 gcgcagctgc ctgcaggtct gagacaataa ccctgataaa tgcttcaata atgtaagctc
      121 taggagatcc gaaccagata agtgaaatct agttccaaac tattttgtca tttttaattt
      181 tcgtattagc ttacgacgct acacccagtt cccatctatt ttgtcactct tccctaaata
      241 atccttaaaa actccatttc cacccctccc agttcccaac tattttgtcc gcccacagcg
      301 gggcattttt cttcctgtta tgtttttaat caaacatcct gccaactcca tgtgacaaac
      361 cgtcatcttc ggctactttt tctctgtcac agaatgaaaa tttttctgtc atctcttcgt
      421 tattaatgtt tgtaattgac tgaatatcaa cgcttatttg cagcctgaat ggcgaatggg
      481 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
      541 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      601 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      661 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      721 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc ttaatagtgg
      781 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
      841 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
      901 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
      961 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     1021 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     1081 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     1141 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     1201 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     1261 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     1321 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     1381 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     1441 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     1501 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     1561 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     1621 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     1681 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     1741 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     1801 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     1861 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     1921 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     1981 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     2041 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     2101 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     2161 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     2221 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     2281 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     2341 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     2401 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     2461 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     2521 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     2581 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2641 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2701 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2761 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     2821 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     2881 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tagaccagcc gcgtaacctg
     2941 gcaaaatcgg ttacggttga gtaataaatg gatgccctgc gtaagcgggt gtgggcggac
     3001 aataaagtct taaactgaac aaaatagatc taaactatga caataaagtc ttaaactaga
     3061 cagaatagtt gtaaactgaa atcagtccag ttatgctgtg aaaaagcata ctggactttt
     3121 gttatggcta aagcaaactc ttcattttct gaagtgcaaa ttgcccgtcg tattaaagag
     3181 gggcgtggcc aagggcatgg taaagactat attcgcggcg ttgtgacaat ttaccgaaca
     3241 actccgcggc cgggaagccg atctcggctt gaacgaattg ttaggtggcg gtacttgggt
     3301 cgatatcaaa gtgcatcact tcttcccgta tgcccaactt tgtatagaga gccactgcgg
     3361 gatcgtcacc gtaatctgct tgcacgtaga tcacataagc accaagcgcg ttggcctcat
     3421 gcttgaggag attgatgagc gcggtggcaa tgccctgcct ccggtgctcg ccggagactg
     3481 cgagatcata gatatagatc tcactacgcg gctgctcaaa cttgggcaga acgtaagccg
     3541 cgagagcgcc aacaaccgct tcttggtcga aggcagcaag cgcgatgaat gtcttactac
     3601 ggagcaagtt cccgaggtaa tcggagtccg gctgatgttg ggagtaggtg gctacgtctc
     3661 cgaactcacg accgaaaaga tcaagagcag cccgcatgga tttgacttgg tcagggccga
     3721 gcctacatgt gcgaatgatg cccatacttg agccacctaa ctttgtttta gggcgactgc
     3781 cctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     3841 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     3901 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     3961 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     4021 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     4081 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     4141 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg ccctggcttc
     4201 aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     4261 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     4321 atagttctag tggttggcta cagcttgcat gcctgcaggc agctgcgcgc tcgctcgctc
     4381 actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg
     4441 agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc ctgcggccgc
     4501 acgcgtggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     4561 ttggggggag gggtcggcaa ttgaaccggc cggtgcctag agaaagtggc gcggggtaaa
     4621 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta
     4681 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca
     4741 ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt
     4801 gccttgaatt acttccacgc ccctggctgc agtacgtgat tcttgatccc gagcttcggg
     4861 ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt
     4921 gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg
     4981 cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga
     5041 cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt
     5101 cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga
     5161 ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc
     5221 ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg
     5281 cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga
     5341 gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga
     5401 aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt
     5461 ccaggcacct cgattagttc tcgatcgagc ttttggagta cgtcgtcttt aggttggggg
     5521 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca
     5581 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc
     5641 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgaaaa
     5701 ctctaggaga attcccataa cttcgtataa agtatcctat acgaagttat atcaaaatag
     5761 gaagaccaat gcttcaccat cgacccgaat tgccaagcat caccatcgac ccataacttc
     5821 gtataatgta tgctatacga agttatacta gctagctcat ttacctccat caccagcgcc
     5881 accagtagag tatctggcca cagccacctc gtgctgctcg acgtaggtct catcgtcggc
     5941 ctccttgatt ctttccagtc tgtggtccac gttgtagacg ccgggcatct tgaggttcgt
     6001 agcgggtttc ttggatctgt atgtggtctc aaggttgcag atcaggtggc ccccgcccac
     6061 gagcttcagg gccatgtcac atgcgccttc caggccgccg tcagcggggt acatcgtctc
     6121 ggtggaggcc tcccagccga gtgttttctt ctgcatcaca gggccgttgg ctgggaagtt
     6181 cacccctcta accttgacgt tgtagatgag gcagccgtcc tggaggctgg tgtcctgggt
     6241 agcggtcagc acgcccccgt cttcgtatgt ggtgactctc tcccatgtga agccctcagg
     6301 gaaggactgc ttaaagaagt cggggatgcc cggagggtgc ttgatgaagg ttctgctgcc
     6361 gtacatgaag ctggtagcca ggatgtcgaa ggcgaagggg agagggccgc cctcgacgac
     6421 cttgattctc atggtctggg tgccctcgta gggcttgcct tcgccctcgg atgtgcactt
     6481 gaagtggtgg ttgttcacgg tgccctccat gtacagcttc atgggcatgt tctccttaat
     6541 cagctcgctc accatgggac cggggttttc ttccacgtct cctgcttgct ttaacagaga
     6601 gaagttcgtg gctccggatc cagactggtt gtgtacgtcc tcccttcgca caatcttgta
     6661 gatgatccaa taaaacatgt tgaaaatcag gaaagccatg gggaatccga tccgggagat
     6721 tttgtcgatc tttttcgctc tctgaataaa gagtttgcgc atttcctccg ggctcttaga
     6781 tggagcaggg ggtgggttgg ttgtgttgct attgttggct cccttcacgg aaattccgtc
     6841 tttagcctgg aggcaagcag gtcccatacc ataggcgcta aagttgaagc gtccttctcc
     6901 tgcctcatcc tctttgtggt ggcgtcgttt tcttctgaac cgcaacagct ctttatgctg
     6961 tcgagacaca aaattgacag cggcgtattc cagcagtgcg ctgaagacaa acagaaggca
     7021 gacagccatc cagatatcga tggccttcac gtaagaaacc ttgggcaagc tggctcggct
     7081 gcctgagctc tgagtagtca ttgtcagcac tgtggtgatg cccagtccga ccctagctgg
     7141 agcggcgtcc atattaatcc agaaggagat ccatgacaaa atcactataa gcaagcttgg
     7201 gatatacatt tggatcagat aataacccat gcgtctccgc atggtcactg tgaaggtgac
     7261 atctgggtag ggctctttgc agcattcata aaatcgctcg cttcttttac cggggattcc
     7321 cacaaggtcc cactccccat tcgggatgta accagagatg tcagcctcct gcatttgcag
     7381 atccaggctc catccgccgt agctccagct cccgaatttg agtttgcagt gctggacgtc
     7441 gaagggaaac cagcggacgt cgatatagca tgaggacttg aagattccag gggggaagta
     7501 ctggcagtgg cctgaagaat tgaccaacac gttggtgtga aatgttgcat caaacctttc
     7561 gtctgcgctg ttgaacagca ggatatctgg tttccagatt tggccgtcag ggaatcttac
     7621 agtctttaca cctgggtatt cgctcacatt ccactgcaag tagtgatcgg tccaggacat
     7681 ctgcaaccaa atattagtgg tcagcacctg gttcttttca tccacgtcca tgatctgcag
     7741 caaggagagg ctgaagtaca ctgtcagtgg ctgggaatca tttgcgaccg gccgctccag
     7801 ggggttataa ttcttaacaa gctccttata cagtttgcgc tggaactcac cctgcaggct
     7861 aacgtgcaga agggaagcag ccagggcgag ccacacgccg cctggagaac agcgcatggt
     7921 ggcggtacct agtataactt cgtataggat actttatacg aagttatcat tgggattctt
     7981 cctattttga tccaagcatc accatcgacc ctctagtcca gatctcacca tcgacccata
     8041 acttcgtata gcatacatta tacgaagtta tgtccctcga agaggttcgc gaagcttatc
     8101 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     8161 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     8221 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     8281 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     8341 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     8401 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     8461 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     8521 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     8581 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     8641 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac
     8701 cgtcgactcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     8761 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     8821 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     8881 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     8941 gctctatggt ccggacacgt gcggaccgag cggccgcagg aacccctagt gatggagttg
     9001 gccactccct ctctgcgcgc tcgctcgctc actgaggccg g
//
