LOCUS       sequence-450966-        6256 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express human FBXL17 in insect cells, such as Trichoplusia ni Hi5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6256)
  AUTHORS   Cao S, Garcia SF, Shi H, James EI, Kito Y, Shi H, Mao H, Kaisari S, 
            Rona G, Deng S, Goldberg HV, Ponce J, Ueberheide B, Lignitto L, 
            Guttman M, Pagano M, Zheng N
  TITLE     Recognition of BACH1 quaternary structure degrons by two F-box 
            proteins under oxidative stress.
  JOURNAL   Cell. 2024 Oct 30:S0092-8674(24)01188-7. doi: 
            10.1016/j.cell.2024.10.012.
  PUBMED    39504958
REFERENCE   2  (bases 1 to 6256)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Oct 30:S0092-8674(24)01188-7. doi: 10.1016/j.cell.2024.10.012."
COMMENT     Sequence Label: pACE-MBP-FBXL17 (310–701)
FEATURES             Location/Qualifiers
     source          1..6256
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  185..350
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     polyA_signal    713..847
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(737..756)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     791..810
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(2224..2244)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(3373..3396)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(3403..3420)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(3577..3686)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        complement(3723..3926)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3752..3772)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3927..4306
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    4384..4417
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        join(4488..4493,4494..4510,4511..4516)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             4705..5238
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     mobile_element  5305..5528
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     rep_origin      complement(5663..6251)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5743..5762)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
       61 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
      121 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttgac ccaagtcaac
      181 cggttgtggg cggacaaaat agttgggaac tgggaggggt ggaaatggag tttttaagga
      241 ttatttaggg aagagtgaca aaatagatgg gaactgggtg tagcgtcgta agctaatacg
      301 aaaattaaaa atgacaaaat agtttggaac tagatttcac ttatctggtt ccaattagat
      361 gggtataccc taggggccgc ccaggccagt acgcatgtac tggtcacgca ttgcactgta
      421 ctcctcttga cctgctgtgt cgagaatatc caagagacag gtttcgccat caattactac
      481 ttgcttacgg taggaatcct cgatggttgg atcatattcg tccacaaaat gattctgaat
      541 cagctggatc gtcaaggcac tcttgcctac gccgtcagct ccaactacca caagtttggc
      601 cagcgcggcc tctaggggtt atgatagtta ttgctcagcg gtggcagcag ccaactcagc
      661 ttcctttcgg gctttgttag cagccggatc ttctaggctc aagcagtgat cagatccaga
      721 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
      781 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
      841 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
      901 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atcctctagt
      961 acttctcgac aagcttgtcg agactgcagg ctctagagga acctgtaccc atgcctcgag
     1021 actcaggcca cactcatcct ccggttatga actagccgca ctcatgttcg gagtccaccc
     1081 catttggtac gcccgttcca gtgttctttt gcagtcttgt aatacggtac tgaaggtaat
     1141 gtgaggatat tgttggacaa gctgttcgac cgtgacctca ttaactttgt cacatcgcat
     1201 caaacccagg tatcgtaaag atttagaact ctgtgcaatt agagtggccc cctggtccgt
     1261 tatctcttta caccacccca cgtctacggt ttcaatggtc atgctatagc gccctatcgc
     1321 gataagtgca taatcagtaa tcttgcagct cactaagtat agctctttca agttctgacc
     1381 ttccttagcg atcacttcga cgcatctgtc atttatgatc caattaagac acaagttcag
     1441 agatgagagg tttttgcaac gcttcacaat ttccattaca gtttcattat ctaactccgt
     1501 aatatgtcgc aaatcgagtg aggacagatt acgcagcttc gtcagatgaa ttacaccctt
     1561 gctggtaact gaacatccca taaagcccac atattggagt tccgggcaat gttcggcgaa
     1621 tgcctttacg gactggtcag ttactaattt gttctcctgc atatatatcc tttgcaattt
     1681 aagacagccc ttagctatta caatcattcc ctcgtccgag atcttgtagc actgtccaaa
     1741 atggatatct ttcaattccc gacacttgct gcccaattgc tttagtccct cgtctgtgag
     1801 cttatcttgg ttacccacgt gaactttttg tagtagagga cagtgggagg caactgcgat
     1861 aatcgatgtg tcgctcagtt gtttacaccg ataggctgtg tatcgtaata agccaggaca
     1921 tttgaaggct aggacgcaaa caccgttatc gctcatggac ctgcagtcgg atatgtttat
     1981 ctcgataata ttttgcgatc tggaagcgat tttttctaat agctcatccg tgacctgttg
     2041 cctactagag aggtcgagct gcttccagaa ctgaaaatct agacagagat cgcgccagta
     2101 cttacaaacc agcgacgcag aaaggcaacg ctcatcaagt gaaagattcg aaaagatctt
     2161 caaaagtata gacgggggga gctgatttat atctggtgtc tcgggtggtg gttcgcggtg
     2221 gcacgattgg aagtagaggt tttcgtcgac agaaccgcta gcagcattag tctgcgcggc
     2281 tgccagggct gcatcgacag tctgacgacc gctggcggcg ttaatcaccg cagtgcgcac
     2341 ggcataccag aaagcggaca tctgcgggat gttgggcatg atttcacctt tctgggcgtt
     2401 ttccattgtg gcggcaacac gtggatcttt caccaactct tcctcgtaag acttcagcgc
     2461 tacggcaccc agcggtttgt ctttattaac cgcttccaga ccttcatcag tcagcaggta
     2521 gttttcgagg aactcttttg ccagctcttt gttcggactg gcggcgttaa tacctgcgct
     2581 cagcacgcca acgaacggtt tggatggttg acccttgaag gtcggcagca ccgttacacc
     2641 ataattcact gcgctggtgt cgatgttgga ccatgcccac gggccgttga tggtcatcgc
     2701 tgtttcgcca tgattaaagg catgttctgc gatggagtaa tcggtgtctg cattcatgtg
     2761 tttgttttta atcaggtcaa ccaggaaggt cagacccgct ttcgcgccag cgttatccac
     2821 gcccacgtct ttaatgtcgt actttccggc tgcatacttg aacgcataac ccccgtcagc
     2881 agcaatcagc ggccaggtga agtacggttc ttgcaggttg aacatcagcg cgctcttacc
     2941 tttcgctttc agttctttat ccagcgccgg gatctcttcc caggtttttg gcgggttcgg
     3001 cagcagatcc ttgttataaa tcagggacaa cgcttcaaca gcgatcgggt aagcaatcag
     3061 cttgccgttg tagcgcacgg catcccaggt aaacggatac agcttgtcct ggaacgctgc
     3121 ggccggggtg atttcagcca acaggccaga ttgagcgtag ccaccaaagc ggtcgtgtgc
     3181 ccagaagata atgtcagggc catcgccagt tgccgcaacc tgtgggaatt tctcttccag
     3241 tttatcggga tgctcaacgg tgactttaat tccggtatct ttctcgaatt tcttaccgac
     3301 ttcagccaga ccgttatagc ctttatcgcc gttaatccag attaccagtt taccttcttc
     3361 ggttttggta cctttatcgt catcgtcttt gtagtcaccg gagtgatggt gatggtggtg
     3421 gtagtacgac atggtttcgg accgagatcc gcgcccgatg gtgggacggt atgaataatc
     3481 cagtatatct ccttcttaag gatccaaaca gatctgttaa atcgacgacg gtgcctgcag
     3541 aaaagaccca ggaaaggaac agtctgtctg tcgagtgatt gtaaataaaa tgtaatttac
     3601 agtatagtat tttaattaat atacaaatga tttgataata attcttattt aactataata
     3661 tattgtgttg ggttgaatta aaggtccgta tactggatgg tgatctgacg gttcactaaa
     3721 cgacgtctgc ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaacggg
     3781 gcggggttat tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa caaactccca
     3841 ttgacgtcaa tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca
     3901 ttggtgtact gccaaaaccg catcaccatg gtaatagcga tgactaatac gtagatgtac
     3961 tgccaagtag gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta
     4021 ccgtcattga cgtcaatagg gggcgtactt ggcatatgat acacttgatg tactgccaag
     4081 tgggcagttt accgtaaata ctccacccat tgacgtcaat ggaaagtccc tattggcgtt
     4141 actatgggaa catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca
     4201 ggcgggccat ttaccgtaag ttatgtaacg cggaactcca tatatgggct atgaactaat
     4261 gaccccgtaa ttgattacta ttaataacta gtcaataatc aatgtcaacg cgtatatctg
     4321 gcccgtacat cgcgatcgat actagtgttt aaactcgcta ccttaggacc gttatagtta
     4381 cagataactt cgtataatgt atgctatacg aagttatggc gacttcgctg ctgcccaagg
     4441 ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg aacccagttg acataagcct
     4501 gttcggttcg taaactgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct
     4561 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt
     4621 tttttgtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat
     4681 gtttgatgtt atggagcagc aacgatgtta cgcagcagca acgatgttac gcagcagggc
     4741 agtcgcccta aaacaaagtt aggtggctca agtatgggca tcattcgcac atgtaggctc
     4801 ggccctgacc aagtcaaatc catgcgggct gctcttgatc ttttcggtcg tgagttcgga
     4861 gacgtagcca cctactccca acatcagccg gactccgatt acctcgggaa cttgctccgt
     4921 agtaagacat tcatcgcgct tgctgccttc gaccaagaag cggttgttgg cgctctcgcg
     4981 gcttacgttc tgcccaagtt tgagcagccg cgtagtgaga tctatatcta tgatctcgca
     5041 gtctccggcg agcaccggag gcagggcatt gccaccgcgc tcatcaatct cctcaagcat
     5101 gaggccaacg cgcttggtgc ttatgtgatc tacgtgcaag cagattacgg tgacgatccc
     5161 gcagtggctc tctatacaaa gttgggcata cgggaagaag tgatgcactt tgatatcgac
     5221 ccaagtaccg ccacctaaca attcgttcaa gccgagatcg gcttcccggc cgcggagttg
     5281 ttcggtaaat tgtcacaacg ccgcgaatat agtctttaca tgcccttggc cacgcccctc
     5341 tttaatacga cgggcaattt gcacttcaga aaatgaagag tttgctttag ccataacaaa
     5401 agtccagtat gctttttcac agcataactg gactgatttc agtttacaac tattctgtct
     5461 agtttaagac tttattgtca tagtttagat ctattttgtt cagtttaaga ctttattgtc
     5521 cgcccacacc cgcttacgca gggcatctgc gcatccacag gaagagcgac ccaagtcaat
     5581 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     5641 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     5701 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     5761 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     5821 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     5881 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     5941 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     6001 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     6061 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     6121 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     6181 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     6241 aaggatctca agaaga
//
