LOCUS       sequence-450968-        5377 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Genetic Incorporation 4-fluorophenyl carbamate lysine (FPheK) into 
            proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Yu C, Tang J, Loredo A, Chen Y, Jung SY, Jain A, Gordon A, Xiao H
  TITLE     Proximity-Induced Site-Specific Antibody Conjugation.
  JOURNAL   Bioconjug Chem. 2018 Nov 21;29(11):3522-3526. doi: 
            10.1021/acs.bioconjchem.8b00680. Epub 2018 Nov 1.
  PUBMED    30372039
REFERENCE   2  (bases 1 to 5377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.bioconjchem.8b00680"; journalName: "Bioconjug Chem"; 
            date: "2018-11-21- 21"; volume: "29"; issue: "11"; pages: 
            "3522-3526"
COMMENT     Sequence Label: pUltra-FPheKRS
FEATURES             Location/Qualifiers
     source          1..5377
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..78)
                     /gene="lacI"
                     /label=lacI promoter
     promoter        join(126..131,132..147,148..154)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    162..178
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(1522..1539)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     regulatory      2159..2168
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(2351..3142)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(3143..3234)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(4295..5377)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     5339..5358
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt
       61 gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattaggg
      121 agctgttgac aattaatcat cggctcgtat aatgtgtgga attgtgagcg gataacaatt
      181 tcacaaagga ggtgcggccg catggataaa aaaccattag atgttttaat atctgcgacc
      241 gggctctgga tgtccaggac tggcacgctc cacaaaatca agcaccatga ggtctcaaga
      301 agtaaaatat acattgaaat ggcgtgtgga gaccatcttg ttgtgaataa ttccaggagt
      361 tgtagaacag ccagagcatt cagacatcat aagtacagaa aaacctgcaa acgatgtagg
      421 gtttcggacg aggatatcaa taattttctc acaagatcaa ccgaaagcaa aaacagtgtg
      481 aaagttaggg tagtttctgc tccaaaggtc aaaaaagcta tgccgaaatc agtttcaagg
      541 gctccgaagc ctctggaaaa ttctgtttct gcaaaggcat caacgaacac atccagatct
      601 gtaccttcgc ctgcaaaatc aactccaaat tcgtctgttc ccgcatcggc tcctgctcct
      661 tcacttacaa gaagccagct tgatagggtt gaggctctct taagtccaga ggataaaatt
      721 tctctaaata tggcaaagcc tttcagggaa cttgagcctg aacttgtgac aagaagaaaa
      781 aacgattttc agcggctcta taccaatgat agagaagact acctcggtaa actcgaacgt
      841 gatattacga aatttttcgt agaccggggt tttctggaga taaagtctcc tatccttatt
      901 ccggcggaat acgtggagag aatgggtatt aataatgata ctgaactttc aaaacagatc
      961 ttccgggtgg ataaaaatct ctgcttgagg ccaatgcttg ccccgactct ttataactat
     1021 gctcgaaaac tcgataggat tttaccaggc ccaataaaaa ttttcgaagt cggaccttgt
     1081 taccggaaag agtctgacgg caaagagcac ctggaagaat ttactatggt gaacttcttt
     1141 cagatgggtt cgggatgtac tcgggaaaat cttgaagctc tcatcaaaga gtttctggac
     1201 tatctggaaa tcgacttcga aatcgtagga gattcctgta tggtctttgg ggatactctt
     1261 gatataatgc acggggacct ggagctttct tcggcagtcg tcgggccagt ttctcttgat
     1321 agagaatggg gtattgacaa accatggata ggtgcaggtt ttggtcttga acgcttgctc
     1381 aaggttatgc acggctttaa aaacattaag agggcatcaa ggtccgaatc ttactataat
     1441 gggatttcaa ccaatctata agcggccgcg tttaaacggt ctccagcttg gctgttttgg
     1501 cggatgagag aagattttca gcctgataca gattaaatca gaacgcagaa gcggtctgat
     1561 aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca cctgacccca tgccgaactc
     1621 agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga gagtagggaa
     1681 ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttg tttgtgagct
     1741 cccggtcatc aatcatcccc ataatccttg ttagcctgca ggtaattccg cttcgcaaca
     1801 tgtgagcacc ggtttattga ctaccggaag cagtgtgacc gtgtgcttct caaatgcctg
     1861 aggccagttt gctcaggctc tccccgtgga ggtaataatt gacgatatga tcagtgcacg
     1921 gctaactaag cggcctgctg actttctcgc cgatcaaaag gcattttgct attaagggat
     1981 tgacgagggc gtatctgcgc agtaagatgc gccccgcatt cggaaacctg atcatgtaga
     2041 tcgaatggac tctaaatccg ttcagccggg ttagattccc ggggtttccg ccaaattcga
     2101 aaagcctgct caacgagcag gcttttttgc atgctcgagc agctcagggt cgaatttgcc
     2161 atggcggcca ccaggtacca ccggcgcctc aggcatttga gaagcacacg gtcacactgc
     2221 ttccggtagt caataaaccg gtaaaccagc aatagacata agcggctatt taacgaccct
     2281 gccctgaacc gacgaccggg tcatcgtggc cggatcttgc ggcccctcgg cttgaacgaa
     2341 ttgttagaca ttatttgccg actaccttgg tgatctcgcc tttcacgtag tggacaaatt
     2401 cttccaactg atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga taagcctgtc
     2461 tagcttcaag tatgacgggc tgatactggg ccggcaggcg ctccattgcc cagtcggcag
     2521 cgacatcctt cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa
     2581 gcactacatt tcgctcatcg ccagcccagt cgggcggcga gttccatagc gttaaggttt
     2641 catttagcgc ctcaaataga tcctgttcag gaaccggatc aaagagttcc tccgccgctg
     2701 gacctaccaa ggcaacgcta tgttctcttg cttttgtcag caagatagcc agatcaatgt
     2761 cgatcgtggc tggctcgaag atacctgcaa gaatgtcatt gcgctgccat tctccaaatt
     2821 gcagttcgcg cttagctgga taacgccacg gaatgatgtc gtcgtgcaca acaatggtga
     2881 cttctacagc gcggagaatc tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt
     2941 tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc agcaaatcaa
     3001 tatcactgtg tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt acggccagca
     3061 acgtcggttc gagatggcgc tcgatgacgc caactacctc tgatagttga gtcgatactt
     3121 cggcgatcac cgcttccctc atactcttcc tttttcaata ttattgaagc atttatcagg
     3181 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagcta
     3241 gctcactcgg tcgctacgct ccgggcgtga gactgcggcg ggcgctgcgg acacatacaa
     3301 agttacccac agattccgtg gataagcagg ggactaacat gtgaggcaaa acagcagggc
     3361 cgcgccggtg gcgtttttcc ataggctccg ccctcctgcc agagttcaca taaacagacg
     3421 cttttccggt gcatctgtgg gagccgtgag gctcaaccat gaatctgaca gtacgggcga
     3481 aacccgacag gacttaaaga tccccaccgt ttccggcggg tcgctccctc ttgcgctctc
     3541 ctgttccgac cctgccgttt accggatacc tgttccgcct ttctccctta cgggaagtgt
     3601 ggcgctttct catagctcac acactggtat ctcggctcgg tgtaggtcgt tcgctccaag
     3661 ctgggctgta agcaagaact ccccgttcag cccgactgct gcgccttatc cggtaactgt
     3721 tcacttgagt ccaacccgga aaagcacggt aaaacgccac tggcagcagc cattggtaac
     3781 tgggagttcg cagaggattt gtttagctaa acacgcggtt gctcttgaag tgtgcgccaa
     3841 agtccggcta cactggaagg acagatttgg ttgctgtgct ctgcgaaagc cagttaccac
     3901 ggttaagcag ttccccaact gacttaacct tcgatcaaac cacctcccca ggtggttttt
     3961 tcgaaggtta agtcagttgg ggaactgctt aaccttcgat caaaccacct ccccaggtgg
     4021 ttttttcgtt tacagggcaa aagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     4081 tgatcttttc tactgaaccg ctctagattt cagtgcaatt tatctcttca aatgtagcac
     4141 ctgaagtcag ccccatacga tataagttgt aattctcatg ttagtcatgc cccgcgccca
     4201 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta
     4261 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     4321 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     4381 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca
     4441 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa
     4501 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt
     4561 atcccactac cgagatgtcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg
     4621 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca
     4681 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta
     4741 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg
     4801 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat
     4861 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct
     4921 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg
     4981 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat
     5041 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc
     5101 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca
     5161 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg
     5221 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt
     5281 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg
     5341 catactctgc gacatcgtat aacgttactg gtttcac
//
