LOCUS       sequence-450969-       14226 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lambda DNA for torsional templates.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14226)
  TITLE     Long DNA for Torsional templates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pMDW133
FEATURES             Location/Qualifiers
     source          1..14226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4962..4978)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4962..4978)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4975..4997)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4986..5002
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5010..5016,5017..5034,5035..5040))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5055..5076
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5193..5210)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5364..5952)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5444..5463)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6123..6914,6915..6983))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6746..6765
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6984..7088)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7156..7174
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7212..7234)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7334..7353
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7547..7569
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7561..7578
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7562..7578
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acctccggca agccctgccg ctttctgcat cagcacatca tcttcaggct cttcgtcagc
       61 ctcgcgccgg ttcagcagac tgaaatccag cggatgcata tccggatcgc tgaaaaacag
      121 gctgagcacg gtgtacgtca gcccggaaaa gtgcatatcc agcagaacat catgaaaata
      181 atgggtactg taaaagcggt gccagtcggc atactccgtg gatgacatcc cggcaagcat
      241 ggcacgccag tcgggtcgcc ccatctcacg cgccagtttc agggcaaaac tcagctcacc
      301 gtcgaacact ttcccgcaga aacaggctct gcgggcccgg cgtcctctgt ctgttcaggg
      361 gcattattca ccacaaactc atacatacca gacagccggt acaccacgtt ttcagcatga
      421 gaaattgcct ccgtgggcca ggtggtaagc acttcctgct caatctgttt aacggcttca
      481 ttcatggacg gcatctgcgt cttctgcgga tggttatgcc acagggacat cgccaccaga
      541 aacgcgccgg ttctgatggc gtcttccaca gtaaacttcc ggttgctgtc tgactccgcc
      601 tgttctgcct gccgtttcat cagggcgaga tgctcaatgc gctgcagggc tgacagttca
      661 gaaagcgtga cggtcacacc gttatgttca aatgattcgg ttttcaggaa catcgctgac
      721 tctccggatt aactggcggt gacggtaatt tctgcaaccg cagcaaactc accattaccg
      781 gatacaaccg gaatgttgac cttgcctgca gcaacgccgt tcacggtgat ggtcatacca
      841 ctgaccgaca cggtggcttt tgttttatcc gcagacaccg cacgaaagct cttgtcggtt
      901 acgccctccg gctggaaggc cacggtcagc gtggtgctct gccctttcac caccgaggtg
      961 ctggcaggcg tcacggtcat gccggttgcc gctgttaccg tgctgcgatc ttctgccatc
     1021 gacggacgtc ccacattggt gactttcacc gtgcgggtga tcacttcctt cgccgtcacc
     1081 gccttaccga tactgctgac ccagccacgg aacacatcga ccgtgccgtt cgggaagcgg
     1141 attttatagg cacgggtatc gccttcatta aaccacgcca gcagcgcctg ctgcccctgc
     1201 tctccgggca tccacgccag cgtgaagctg gtatctccgg cagatttctg cccctgcccg
     1261 gtcgcagtcc agtctgcatc ttcatcatcg agatagctgt cgtcatagga ctcagcggtc
     1321 agttcgccgg gcgtcaggtc tttaactttt gccagacgcg accagtcaac gtctgaaagc
     1381 ggattcgcgt aagggtcacc gctcccctta taaacccaca gggtggtccc ggcacctttc
     1441 accggcattg taggatttgg tacaggcata gcgtcctcac atttcatagg taatgacata
     1501 agtcagatcg gctgaactcc acaagcccgc atcatcgtcg cgccggtagt catagccgct
     1561 ggccaccata ctggtgatca aatctgacag tgccgggata tcgctcatca ccggataaat
     1621 ccgggactcc atccacgcat ccagctctga atccggcacc tgagcaggca ggaaaacttc
     1681 gatatgcagc tccgcctgcc aggtatcgct gtccagctct tcgcccgtgt attcagcgcc
     1741 ggtgagataa acggcaactg ccggaaaatc cgcctcatca aaaacagcgg ggcgaccatc
     1801 aaaaaacgtc gccccggtgt catgcttctc cagtgcatcc agtacggctg cacggagttc
     1861 agtatgtttc atcgctttat taccatcctc agttgatgct gcagcgcata gcccagctct
     1921 ttcggaagac gttcacgccg tatccgctca atattttgtt taaacgccgt ggtcagcggc
     1981 accgccatcg ggattttcac cacatcaatg gggtaacggt ttttcccagc cacacgctgc
     2041 atgacatgcc accggccatt tttcagttgc tgaataaacg cgccgggaat acgacggtta
     2101 cccaccacaa gcacgctgcc gccacctttc agggatgaac gctgcccctt tttacgacgc
     2161 ctgcggcgcg aaaggacaac ccgcgcatta cccagcttga ttacgggcaa atccccccgg
     2221 ttaactttga ttctggcctg cggatttttg accgtggccc ttttcagcct ggccctttcc
     2281 tttaccagtt tccggcgtac ctttgtctca cgggcaacct gtgacgccga ctgcgatatc
     2341 gcggatgaag caacgcggtt aatggccatt gcggcggcac caggcaccgc cgttttgctg
     2401 atacggctga ggttttcaac ggcctgctca agacctttta tggccataca tccccctttc
     2461 agcggcgacg gttaacggca ggcggtacgc cccgtccaag ccagagatga caacttccgc
     2521 catcatccgg cgaaacccga tctacccaga aattttcctc accgatggtc agcgtgtctc
     2581 cacgccgcag ctgccgcacc tcatcagtcc ggacaaacag ggacgggctg gagccttcaa
     2641 cgcgcacgcc ctgtccggca tagctgatat tttcagggtc atcaaaaaca ccacgtatca
     2701 ccgcacctga ctgctcaccg gatgtaatgg tggctgacgt tcccatgtac ccgcgtatcg
     2761 tttcatcggc gcgggcaatg gcagcatcga acaggttatc gaaatcagcc acagcgcctc
     2821 ccgttattgc attctggcca ggccgcgctc tgtcatttcg gctgccacac cggcagagac
     2881 acgaaacgcc gttcccggca gcacaaatgc cacaggttca tcccgcgtgg cgtgaagtgc
     2941 atcagtatgc agcttcacca gtgccacgac cgtgaccagt tcagacgtat ccagaatcac
     3001 ggtatccggc tgcgctgatc ccacctcatt ttcatgtccg gtcagcacat tttcccggct
     3061 gagaggggtg tcctgaccgg cagtttcatc cgtgtcatca agctcctctt tcagctctgc
     3121 cacacggagc gccagttctt ctttcgtccc cgtcaggctg acatcacggt tcagttgttc
     3181 acccagcgag cggagacggg caatcagttc atctttcgtc atggactcct ccacagagaa
     3241 acaatggccc cgaagggcca tgattacgcc agttgtacgg acacgaactc atcagggtca
     3301 gccagcagca tcagcggtgc tgactgaatc atggtgaact cacgcgccgg atcgccggtg
     3361 gtcacccagt ttttcgggta acgggcagag gcgttaatgc cttcgcgctg tgcgtccgca
     3421 tcctgaatgc agccataggt gcgcagaccg cgtgcctgag tgttccccag caccatcgtg
     3481 ttgtccggca ggaagttctt tttgacgccg ttttccacgt actgtccgga atacacgacg
     3541 atggccacat cgccatacat ccccttatag gacaccgctt tgcccaggtc tttcaccgct
     3601 gtctccagct cggaattaga gccacgacgg gtatccagct tctccttgac ggctttgaag
     3661 gaacggaaca gcgcccagcc tttcggatcg aacacgatga tattcaccac accgctggcg
     3721 ttcagcgcgt aggcttcgat atcgtcggtc gggtcatacg tggacttgtc acgcttgctc
     3781 cactccgtgc cgccggactg cgtgatgtta ttctcctcac tgcggcccat atccacctca
     3841 accggatcga aggcttcacc ggtcatggtg tatttgccct taagcacggc agaaactgcc
     3901 tgcatctctt cgacctgagc aatggccagc tcttcgtcac gcatgttctg catgatgatg
     3961 cgacggcggc ggtaagccgg gtccgccaga ttctgcggat cttcatccgg caggcgacgc
     4021 agggtcatct gcggattcac ttcatgcttc ggcttgacat atcccggcgt aaattcagag
     4081 gtggagccgc cacgggaacg gataacctca ccggaaacaa tcggcgaaac gtacagcgcc
     4141 atgtttacca gtcccggaat ttgtgagaga tagactttct ccgtggtgaa gggatagctc
     4201 tcacggaaaa agagacgcag aaacagcgga tcaaacttaa atttctgctc atttgccgcc
     4261 agcagttggg cggttgtgta catcgacata aaaaaatccc gtaaaaaaag ccgcacaggc
     4321 ggcctttagt gatgaagggt aaagttaaac gatgctgatt gccgttccgg caaacgcggt
     4381 ccgttttttc gtctcgtcgc tggcagcctc cggccagagc acatcctcat aacggaacgt
     4441 gccggacttg tagaacgtca gcgtggtgct ggtctggtca gcagcaaccg caagaatgcc
     4501 aacggcagca ccgtcggtgg tgccatccca cgcaaccagc ttacggctgg aggtgtccag
     4561 catcagcggg gtcattgcag gcgctttcgc actcaatccg ccgggcgcgg ttgcggtatg
     4621 agccgggtca ctgttgccct gcggctggta atgggtaaag gtttctttgc tcgtcataaa
     4681 catcccttac actggtgtgt tcagcaaatc gttaacggca tcagatgccg ggttacctgc
     4741 agccagcggt gccggtgccc cctgcatcag acgatccagc gcagtgtcac tgcgcgcctg
     4801 tgcactctgt ggtgctgcgg ccagaatgcg gcgggccgtt ttcacggtca taccgggggt
     4861 ttctgccagc acgcgtgcct gttcttcgcg tccgtgagcc tcctcacagt tgaggatcct
     4921 ctagagtcga cctgcaggca tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg
     4981 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     5041 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     5101 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     5161 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     5221 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     5281 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     5341 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     5401 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     5461 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     5521 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     5581 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     5641 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     5701 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     5761 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     5821 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     5881 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     5941 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     6001 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     6061 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     6121 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     6181 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     6241 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     6301 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     6361 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     6421 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     6481 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     6541 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     6601 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     6661 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     6721 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     6781 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     6841 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     6901 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     6961 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     7021 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     7081 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     7141 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat
     7201 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     7261 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     7321 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
     7381 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg
     7441 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
     7501 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     7561 tgtaaaacga cggccagtga attcgagctc ggtaccggag gatggcagcg tgatttcacg
     7621 gtcgagcgtc agcgtccggg tctggctgtt caccgccagc acacgaccac cggtgctgat
     7681 accggcatag tcatcatcgc agatttcaat aacatcgccc ggtacatggc gaagcccttc
     7741 tgcgccgacg ctgaaatcca cggtctgcgt ttccagcagt tctgttttaa tcagccacag
     7801 cccggcgcgg tgtgcctgcc cccggctggt acagccaaag gcatccatct tcgtaacatt
     7861 acgaccgtaa cgggcaatgg cctgcgtatc ttcaacaagc tctgtcgccg tctcccagcc
     7921 gttgttcggg tcaatccagt tcacctcaac ggcattatgg cggtccttca gggcgctgaa
     7981 gctgtagcgg aacggcgcgc catcatccgg catcaccaca ttactgcggt tataggtcca
     8041 cgtcttatcc gacggtcggt cctgcacgaa cgtcagcgtc tgcccgttcc ataccggcat
     8101 acagcgcatc gccgagcaga aatcgctgag cacatcccac gccttacgct gtgtggtcag
     8161 gtacgcatta caggtgatgc gcggctccgt gccgccaaag ccgtccggca ctgactggtc
     8221 gcagtactgg ccgatgacat acagcgccca tttatccaca tccgccgcac caagacgttt
     8281 ccccatgccg tagcgcggat gggtcagcat atcccacaga caccaggcca tgttgttgct
     8341 gtatgccggt ttaaacgttc cgtcccagat accgctgtat tgccgcgtct gcgggttata
     8401 gttcgacggc acctgcagaa tacgcccgcg cagatgataa ttacggctca cctgctggct
     8461 gccgaactgc tccgagtcca cctgcacgcc gaccagtgcc gtgttcgggt agcactgttt
     8521 cacatcgatg atttcagtgt atgacgacca gagcgttttg ttctgcagct ggtctgtggt
     8581 gctgtccggc gtcatcctgc gcatccggat attaaacggg cgcggcggca ggttacccat
     8641 caccaccgag gccagatact gcgaggtggt tttgccctta atggtgatgt ctttttccgt
     8701 cacccagcca ccgttacgtt gtatctgaac cagcaggcgg acttccgacg gattcctgtc
     8761 accctttgag gtggtttcca ccagtgcctg tacaccgaag gtaaagcgca gacggtcgat
     8821 gtttgcagac gtaatggtgc gggtgatcgg cgtgtcatat ttcacttccg tacccagcac
     8881 cgtctcggag ccggaggatt caaatccctc cggcggagtc tgctcctgct caccagcccg
     8941 gaacaccacc gtgacaccgg atatgttggt attcccctca gtgtccagca ccggcgtact
     9001 gttcagcagc acgcttttta agccatccac cggaccttca atcggccctt cgctgatggc
     9061 atcgatcaca ctcagcaact gcgtggactt caggttgtcc ttcgcttcgc gcggggtatg
     9121 ccccttactg cttcctttac ccattcctca cgctccataa atgacaaaac cgcccgcagg
     9181 cggtttcaca taaaacattt tgcatcagcg accaatcacc acaacctgac caccgtcccc
     9241 ttcgtctgcc gtgctgatct cctgagaaac cacgcgtgac cccacgcgca tttccccgta
     9301 cagaacaggc agaacattgc cctgggcaac catgttatcc agtgaggaga aataggtgtt
     9361 ctgcttaccg ttatccgttg tctgtatacg gggagttctg gctttcggtg ccagcatctg
     9421 cgccacacca ccgagcacca tactggcacc gagagaaaac aggatgccgg tcataccacc
     9481 ggccccaatg gctgcccccc atgctgcaag ggtggctccg gcggtaaaga atgatccggc
     9541 aatggcggca gcccccagga caatctggaa tacgccacct gacttggccc cggcgactct
     9601 gggaacaata tgaattacag cgccatcagg cagagtctca tgtaactgcg ccgttaaccc
     9661 ggacgtgctg acgtcccgcc cggcaatccg tacctgatac cagccgtcgc tcagtttctg
     9721 acgaaacgcc gggagctgtg tggccagtgc ccggatggct tcagcccccg ttttcacacg
     9781 aaggtcgatg cggcgaccaa atcgttgtaa atccccgtaa aggcagatgc gcgccatgcc
     9841 cggtgacgcc agagggagtg tgtgcgtcgc tgccatttgt cggtgtacct ctctcgtttg
     9901 ctcagttgtt caggaatatg gtgcagcagc tcgccgtcgc cgcagtaaat tgcggcgtga
     9961 ttcggcactg atgaaccaaa acagcacagc agcacatcgc ccggctgtgc cgctgacaac
    10021 ggcacctgat acagccccgt cgcctccaga ttatccagat agagattctg gccgttacgc
    10081 caccagtcat cctcacgatg aaagtccggc atctcaatcc ccgccagatg ataagcatcc
    10141 cggaacagtg tgtaacagtc cgtcacaccg tgctcaaagc gccgcccggt gagatgcggc
    10201 acacagcgga acttatgaat cgtcccccgg cagaccagcc accacggcaa atcactctgc
    10261 acctgcagcc gccggtcggc ctcactcagc cagggcagac caccggggtg gctgtggacc
    10321 agcgccacaa tctcaccctg catttctgcc tgcagccagt cttccggcga catacggaaa
    10381 tacgcctccg gctcaccgga gatattcacg caggggaaat atctttcccc ctccggcgtg
    10441 cttaccacga agccgcacga ctccgctggc gcacatcgcc gggcgtgcgc cagaatcgct
    10501 gattctgtct gtgtcatggg atttactgcg aaagtttgtt aatggaaagg aagccgccaa
    10561 agttgccgac gttattgcgg aacttacaac cgctcaggca tttgctgcat ttatccttcg
    10621 tgatatcgga cgttggctgg tcatattcat ccgcgacagc cggaccgcta taaccgcact
    10681 cgtcaccgcg ataggtccag gtgcaggtgt tggccagcat gatacgtccc ggaaaaacag
    10741 cgccatccgt ttccgtcggc gtggacagta caaaggaggc actcaccgcg ctcagttcgc
    10801 tgcactgctc aatgcgccag cggctgatca cctcctgctc cggatcggcg taactgtttc
    10861 cgttgacgaa gttcaccgca tccagaaaac gggcgtaaac cttacgccgg accaccgttc
    10921 cgccgaccag actctgcata tcttccgcca tcccggtgac cataccgtac aggttagaaa
    10981 ccgtcagcgt ggggcgcgta ctggtgcctt tgccattcag ttcaaaaccg ctcccctgaa
    11041 tgggatacgg ctgatactgt cgcccctgcc aggtgaccgg ctcacctttt tcgttctgct
    11101 cattacagaa aaaataacgt tctccaccga cctctgtcag gtcgatttcc cagagcacca
    11161 cgctggccga ctgctccgca cgggtgcatt cattcagtgt ttcctgccgg atatcctgca
    11221 tcagttcacc acctgttcaa actctgcgct gaactcaaca cgcagcatac tgacccgcga
    11281 cgaccatttt gcgcaggtca cctttatctg ccgccactca taaggcggcg tccacagaaa
    11341 ggatttccag cccccgtgct cttccagaaa cgactccagt accgtggcct cctcacgggg
    11401 gacagaaagc gtcacgctgt acgttttcag gttggcattc agcccggcag gcgctcgctg
    11461 agaatagcca tcaccaaagc gcacctttct tacagaaggg accgaagcca catccatacc
    11521 gggtttcact ttccagcgga aggtcttcat cgtccacctc cggagaacag gccaccatca
    11581 cgcatctgtg tctgaatttc atcacgggca cccttgcggg ccatgtcata caccgccttc
    11641 agagcagccg gacctatctg cccgttcgtg ccgtcgttgt taatcaccac atggttattc
    11701 tgctcaaacg tcccggacgc ctgcgaccgg ctgtctgcca tgctgcccgg tgtaccgaca
    11761 taaccgccgg tggcatagcc gcgcatcagc cggtaaagat tccccacgcc aatccggctg
    11821 gttgcctcct tcgtgaagac aaactcacca cggtgaacaa tccccgctgg ctcatatttg
    11881 ccgccggttc ccgtaaatcc tccggttgca aaatggaatt tcgccgcagc ggcctgaatg
    11941 gctgtaccgc ctgacgcgga tgcgccgcca ccaacagccc cgccaatggc gctgccgata
    12001 ctcccgacaa tccccaccat tgcctgctta agcagaattt ctgtcatcat ggacagcacg
    12061 gaacgggtga agctgcgcca gttctgctca ctgccggtca gcatcgccgc catattctgt
    12121 gcaataccat caaaggtctg cgtggctgca ctttttacct gcgacatact gtccgtggcg
    12181 ctctcttccc actcactcca gccggacttc aggcctgcca tccagttccc gcgaagctgg
    12241 tcttcagccg cccaggtctt tttctgctct gacatgacgt tattcagcgc cagcggatta
    12301 tcgccatact gttccttcag gcgctgttcc gtggcttccc gttctgcctg ccggtcagtc
    12361 agcccccggc ttttcgcatc aatggcggcc cgttttgccc gttgctgctg tgcgaattta
    12421 tccgcctgct gcgccagcgc gttcaggcgc tcctgatacg taaccttgtc gccaagtgca
    12481 gccagctggc gtttgtactc cagcgtctca tctttatgcg ccagcaggga tttctcctgt
    12541 gcagacagct ggcgacgttg cgccgcctcc tccagtaccg cgaactgact ctccgccttc
    12601 cacaaatccc ggcgctgctg gctgattttc tcatttgctc cggcatgctt ctccagcgtc
    12661 cggagttctg cctgaagcgt cagcagggca gcatgagcac tgtcttcctg acgatcgccc
    12721 gcagacacct tcacgctgga ctgtttcggc tttttcagcg tcgcttcata atcctttttc
    12781 gccgccgcca tcagcgtgtt gtaatccgcc tgcaggattt tcccgtcttt cagtgccttg
    12841 ttcagttctt cctgacgggc ggtatatttc tccagcggcg tctgcagccg ttcgtaagcc
    12901 ttctgcgcct cttcggtata tttcagccgt gacgcttcgg tatcgctctg ctgctgcgca
    12961 tttttgtcct gttgagtctg ctgctcagcc ttctttcggg cggcttcaag cgcaagacgg
    13021 gccttttcac gatcatccca gtaacgcgcc cgcgcttcat cgttaacaaa ataatcatcc
    13081 ttgcgcagat tccagatgtc gtctgctttc ttatacgcag cctctgcctt aatcagcatc
    13141 tcctgcgcgg tatcaggacg accaatatcc agcaccgcat cccacatgga tttgaatgcc
    13201 cgcgcagtcc tgtctgccca ggtctccagc gtgcccatgt tctctttcag gcggcgggtc
    13261 tggtcatcaa accctttcgt tgcggcctcg ttcgccgcct gcaatgcccc ggcttcatcg
    13321 ccggaacgct gcaactgagc aacatacgca atctgctccg ccgacacgtt atggaactgg
    13381 cgagccatcg ccgtcagccc cgacgtcggg tctgtggtca gcttcccgaa ggcttcagcg
    13441 accttgtcca cctccacgcc ggatgcagag gagaaacgcg ccacactctg gctgatggac
    13501 gcaatctgag cctcaccgct tacccccgcc ttaaccagtg cgctgagtga ctcgctggtc
    13561 tggttaaacg tcagccctgc cgcctgcccg gctctggaca ggaccagcat acgatctgcc
    13621 gtcagtcccg cctgattgcc ggaaaggacc agcgttttgt tgaaatcgga cagggttgag
    13681 ttgccctgat accaggcata cgccagcgca ccggtcgcca ccgccagcga ggtggccccc
    13741 accatcggca gggtgatcgc accggcaagc cccctgaaca tggggatcat cccgccgaag
    13801 gagtccttca cctgcccccc ctgttgcagc aggatcagcc acggactttg cccgcctgca
    13861 agctgcgtgg ccacgtcggt gaactgtgca ggcagcatac gcatggcggc tttatactgc
    13921 ccgacggaaa tccccgcttt ctgtgcagcc agcgcctgtc ggctcagcga ctgttcaacg
    13981 actgccgctg tttttttcgc atcactttcc gtaccagaaa aatgacgcct gactctggcc
    14041 atctgctcgt caaatctggc cgcatccaga ctcaaatcaa cgaccagatc gcctaccggt
    14101 tcagccatac cggactcctc ctgcgatccc ttctgatact gtcatcagca ttacgtcatc
    14161 ctccgtcatg tccgccacat ccggggaagc ggggataact tcattcccgt ccgggccaaa
    14221 gcggac
//
