LOCUS       sequence-450977-        8985 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of PE6a prime editor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8985)
  AUTHORS   Doman JL, Pandey S, Neugebauer ME, An M, Davis JR, Randolph PB, 
            McElroy A, Gao XD, Raguram A, Richter MF, Everette KA, Banskota S, 
            Tian K, Tao YA, Tolar J, Osborn MJ, Liu DR
  TITLE     Phage-assisted evolution and protein engineering yield compact, 
            efficient prime editors.
  JOURNAL   Cell. 2023 Aug 31;186(18):3983-4002.e26. doi: 
            10.1016/j.cell.2023.07.039.
  PUBMED    37657419
REFERENCE   2  (bases 1 to 8985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2023.07"; journalName: "Cell"; date: "2023-08-31- 
            31"; volume: "186"; issue: "18"; pages: "3983-4002.e26"
COMMENT     Sequence Label: pCMV-PE6a
FEATURES             Location/Qualifiers
     source          1..8985
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(3409..3429)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(3478..3497)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3479..3497)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3539..3742)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3568..3588)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3743..4122
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     4294..4313
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4392..4496
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4497..4565,4566..5357)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4715..4734)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5528..6116
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6017..6036
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6270..6287
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6404..6425
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6440..6445,6446..6463,6464..6470)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6478..6494
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6483..6505
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6502..6518
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6502..6518
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    6589..6813
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     6802..6819
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(6842..6859)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(6868..6894)
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             complement(6904..6924)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(8215..8235)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(8293..8985,1..3408))
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 ggggctgtcg aagccgccgt acttcttagg gtcccagtcc ttctttctgg cgatcagctt
       61 atcgctgttc ctcttgggca ggatagactc tttgctgaag ccgcctgtct gcacctcggt
      121 ctttttcacg atattcactt ggggcatgct cagcactttc cgcacggtgg caaaatcccg
      181 gcccttatcc cacacgatct ccccggtttc gccgtttgtc tcgatcagag gccgcttccg
      241 gatctcgccg ttggccaggg taatctcggt cttgaaaaag ttcatgatgt tgctgtagaa
      301 gaagtacttg gcggtagcct tgccgatttc ctgctcgctc ttggcgatca tcttccgcac
      361 gtcgtacacc ttgtagtcgc cgtacacgaa ctcgctttcc agcttagggt actttttgat
      421 cagggcggtt cccacgacgg cgttcaggta ggcgtcgtgg gcgtggtggt agttgttgat
      481 ctcgcgcact ttgtaaaact ggaaatcctt ccggaaatcg gacaccagct tggacttcag
      541 ggtgatcact ttcacttccc ggatcagctt gtcattctcg tcgtacttag tgttcatccg
      601 ggagtccagg atctgtgcca cgtgctttgt gatctgccgg gtttccacca gctgtctctt
      661 gatgaagccg gccttatcca gttcgctcag gccgcctctc tcggccttgg tcagattgtc
      721 gaactttctc tgggtaatca gcttggcgtt cagcagctgc cgccagtagt tcttcatctt
      781 cttcacgacc tcttcggagg gcacgttgtc gctcttgccc cggttcttgt cgcttctggt
      841 cagcaccttg ttgtcgatgg agtcgtcctt cagaaagctc tgaggcacga tagcgtccac
      901 atcgtagtcg gacagccggt tgatgtccag ttcctggtcc acgtacatat cccgcccatt
      961 ctgcaggtag tacaggtaca gcttctcgtt ctgcagctgg gtgttttcca cggggtgttc
     1021 tttcaggatc tggctgccca gctctttgat gccctcttcg atccgcttca ttctctcgcg
     1081 gctgttcttc tgtcccttct gggtggtctg gttctctctg gccatttcga tcacgatgtt
     1141 ctcgggcttg tgccggccca tcactttcac gagctcgtcc accaccttca ctgtctgcag
     1201 gatgcccttc ttaatggcgg ggctgccggc cagattggca atgtgctcgt gcaggctatc
     1261 gccctggccg gacacctggg ctttctggat gtcctcttta aaggtcaggc tgtcgtcgtg
     1321 gatcagctgc atgaagtttc tgttggcgaa gccgtcggac ttcaggaaat ccaggattgt
     1381 cttgccggac tgcttgtccc ggatgccgtt gatcagcttc cggctcagcc tgccccagcc
     1441 ggtgtatctc cgccgcttca gctgcttcat cactttgtcg tcgaacaggt gggcataggt
     1501 tttcagccgt tcctcgatca tctctctgtc ctcaaacagt gtcagggtca gcacgatatc
     1561 ttccagaatg tcctcgtttt cctcattgtc caggaagtcc ttgtccttga taattttcag
     1621 cagatcgtgg tatgtgccca gggaggcgtt gaaccgatct tccacgccgg agatttccac
     1681 ggagtcgaag cactcgattt tcttgaagta gtcctctttc agctgcttca cggtcacttt
     1741 ccggttggtc ttgaacagca ggtccacgat ggcctttttc tgctcgccgc tcaggaaggc
     1801 gggctttctc attccctcgg tcacgtattt cactttggtc agctcgttat acacggtgaa
     1861 gtactcgtac agcaggctgt gcttgggcag caccttctcg ttgggcaggt tcttatcgaa
     1921 gttggtcatc cgctcgatga agctctgggc ggaagcgccc ttgtccacca cttcctcgaa
     1981 gttccagggg gtgatggttt cctcgctctt tctggtcatc caggcgaatc tgctgtttcc
     2041 cctggccaga gggcccacgt agtaggggat gcggaaggtc aggatcttct cgatcttttc
     2101 ccggttgtcc ttcaggaatg ggtaaaaatc ttcctgccgc cgcagaatgg cgtgcagctc
     2161 tcccaggtgg atctggtggg ggatgctgcc gttgtcgaag gtccgctgct tccgcagcag
     2221 gtcctctctc ttcagcttca cgagcagttc ctcggtgccg tccatctttt ccaggatggg
     2281 cttgatgaac ttgtagaact cttcctggct ggctccgccg tcaatgtagc cggcgtagcc
     2341 gttcttgctc tggtcgaaga aaatctcttt gtacttctca ggcagctgct gccgcacgag
     2401 agctttcagc agggtcaggt cctggtggtg ctcgtcgtat ctcttgatca tagaggcgct
     2461 caggggggcc ttggtgatct cggtgttcac tctcaggatg tcgctcagca ggatggcgtc
     2521 ggacaggttc ttggcggcca gaaacaggtc ggcgtactgg tcgccgatct gggccagcag
     2581 gttgtccagg tcgtcgtcgt aggtgtcctt gctcagctgc agtttggcat cctcggccag
     2641 gtcgaagttg ctcttgaagt tgggggtcag gcccaggctc agggcaatca ggtttccgaa
     2701 caggccattc ttcttctcgc cgggcagctg ggcgatcaga ttttccagct ttctgctctt
     2761 gctcagtctg gcagacagga tggccttggc gtccacgccg ctggcgttga tggggttttc
     2821 ctcgaacagc tggttgtagg tctgcaccag ctggatgaac agcttgtcca cgtcgctgtt
     2881 gtcggggttc aggtcgccct cgatcaggaa gtggccccgg aacttgatca tgtgggccag
     2941 ggccagatag atcagccgca ggtcggcctt gtcggtgctg tccaccagtt tctttctcag
     3001 gtggtagatg gtggggtact tctcgtggta ggccacctcg tccacgatgt tgccgaagat
     3061 ggggtgccgc tcgtgcttct tatcctcttc caccaggaag gactcttcca gtctgtggaa
     3121 gaagctgtcg tccaccttgg ccatctcgtt gctgaagatc tcttgcagat agcagatccg
     3181 gttcttccgt ctggtgtatc ttcttctggc ggttctcttc agccgggtgg cctcggctgt
     3241 ttcgccgctg tcgaacagca gggctccgat caggttcttc ttgatgctgt gccggtcggt
     3301 gttgcccagc accttgaatt tcttgctggg caccttgtac tcgtcggtga tcacggccca
     3361 gcccacagag ttggtgccga tgtccaggcc gatgctgtac ttcttgtcga ctttccgctt
     3421 cttctttggt gactcgaact cgcttccgtc ggctgtccgt ttcatggtgg cggctctccc
     3481 tatagtgagt cgtattagcg gccgcggatc tctagcggat ctgacggttc actaaaccag
     3541 ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg
     3601 agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga
     3661 cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga
     3721 tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc
     3781 aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt
     3841 cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg
     3901 cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta
     3961 tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg
     4021 ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc
     4081 ccgtaattga ttactattaa taactagtca ataatcaatg tcaacgcgta tatctggccc
     4141 gtacatcgcg aagcagcgca aaacgcctaa ccctaagcag attcttcatg caattgtcgg
     4201 tcaagccttg ccttgttgta gcttaaattt tgctcgcgca ctactcagcg acctccaaca
     4261 cacaagcagg gagcagatac tggcttaact atgcggcatc agagcagatt gtactgagag
     4321 tcgaccctag gggatcggga gatcgatctc ccgatccgtc gacgtcaggt ggcacttttc
     4381 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     4441 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     4501 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     4561 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     4621 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     4681 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     4741 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     4801 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     4861 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     4921 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     4981 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     5041 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     5101 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     5161 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt ggttctcgcg
     5221 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     5281 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     5341 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     5401 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     5461 aaatccctta acgtgagttt tcgttccact gagtgtcaga ccccgtagaa aagatcaaag
     5521 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     5581 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     5641 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     5701 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     5761 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     5821 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     5881 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     5941 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     6001 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     6061 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     6121 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     6181 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     6241 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     6301 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     6361 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     6421 ctcattaggc accctaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     6481 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagctctagc
     6541 tagaggtcga cggtatcgag ccccagctgg ttctttccgc ctcagaagcc atagagccca
     6601 ccgcatcccc agcatgcctg ctattgtctt cccaatcctc ccccttgctg tcctgcccca
     6661 ccccaccccc cagaatagaa tgacacctac tcagacaatg cgatgcaatt ttctcatttt
     6721 attaggaaag gacagtggga gtggcacctt ccagggtcaa ggaaggcacg ggggaggggc
     6781 aaacaacaga tggctggcaa ctagaaggca cagtcgaggc tgatcagcgg gtttaaactc
     6841 aatggtgatg gtgatgatga ccggttagtc cagcttcact ctcttagcgg cagggccaga
     6901 gccgactttc ctcttcttct tgggagactc gaattcgctg ccgtcggcgg ttctttttga
     6961 gccgccagac aggggcttca ggctagccag ccgggactta aaacattcga gcttctcttt
     7021 gaagctctcg cttctggtca agatgatcat cttgtatctg gtcagatcat acttcctctt
     7081 gtaccagtgc ttgttctgat tgcccttgct gtagctcagc tcaaggctct tgatagcggc
     7141 gtcgatcacg gccacgtccc gattgctagg cataggcttg atggcctgca gctgcttttt
     7201 aaagctctcg tacttctcgt ggcccacccg gcccagctcg ttcactctgc ccatgcatct
     7261 gttgaattcc tttctgtagg ccacgctggt cttggtgttg ttcttggcgg ccagcagctt
     7321 caagttgtgg atgctggccc ggatgcgctt caccttatca gaaggcagtc tggggctgtc
     7381 gtagtcaact atcaggccgt gcaccttgat aggctcgctg ctacagtgga agatcttggt
     7441 cttgtgcttg ttgatgggca ggttgtgctc agacagcatc cgctcgatgt ggctctgcat
     7501 ctggctgaag tcgtagttgg agattttaga agacacggtg atgtcatcca ccagtcttgt
     7561 gtacaccagt ccctttctct gggcccgccg caccacgtcg ccttccacgg caaacaggca
     7621 cagggtggcg atataagagc ttgtcagggc tccctggacc acgaagtcgt cctttgtgca
     7681 gatatcgacc aggtaatcca gggcctcatc tttgatgtgc aggatttcct cgaacacaga
     7741 tctgaccaga tctctatgga tgttgtcgaa gaagttgctg atgtccactt tcagtacggt
     7801 tttggcgccg cagtgggcct tggcgcagga cacgtaatcc cgcttcacat tgctgttcag
     7861 cacgtcattt ttgctaggca cggagccgaa cagaaaagat gggaacacca ccagttcctt
     7921 gaagattctt tcgttgattc tgctttgcag cagccgcatc ttaggatgga gggagtacac
     7981 aatcctcttg ctgccgtcga tcttggggat tttcttcagt gtgtatttct cgtccaggct
     8041 cagttcggcg atagccttca gctcctctac gctgatggac agagctttgc tcagcttctc
     8101 cagggtggta ataggggcat tagatttgct gtatggcttg aacttgtcca tgaacatgcc
     8161 gttcagattc agtgtaacgt aaggtcttcc gcttccgccg ctagagcctc cgctcacctt
     8221 tcttttcttc ttagggctct cgaattcaga gccgtcggcg gtccgcttgc tgccaccaga
     8281 gcttccgccg gagtcacctc ccagctgaga caggtcgatc cgtgtctcgt acaggccggt
     8341 gatgctctgg tggatcaggg tggcgtccag cacctctttg gtgctggtgt acctcttccg
     8401 gtcgatggtg gtgtcaaagt acttgaaggc ggcaggggct cccagattgg tcagggtaaa
     8461 caggtggatg atattctcgg cctgctctct gatgggctta tcccggtgct tgttgtaggc
     8521 ggacagcact ttgtccagat tagcgtcggc caggatcact ctcttggaga actcgctgat
     8581 ctgctcgatg atctcgtcca ggtagtgctt gtgctgttcc acaaacagct gtttctgctc
     8641 attatcctcg ggggagccct tcagcttctc atagtggctg gccaggtaca ggaagttcac
     8701 atatttggag ggcagggcca gttcgtttcc cttctgcagt tcgccggcag aggccagcat
     8761 tctcttccgg ccgttttcca gctcgaacag ggagtactta ggcagcttga tgatcaggtc
     8821 ctttttcact tctttgtagc ccttggcttc cagaaagtcg atgggattct tctcgaagct
     8881 gcttctttcc atgatggtga tccccagcag ctctttcaca ctcttcagtt tcttggactt
     8941 gcccttttcc actttggcca ccaccagcac agaataggcc acggt
//
