LOCUS       sequence-45098-e        2504 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Seeliger JC, Topp S, Sogi KM, Previti ML, Gallivan JP, Bertozzi CR
  TITLE     A riboswitch-based inducible gene expression system for 
            mycobacteria.
  JOURNAL   PLoS One. 2012;7(1):e29266. Epub 2012 Jan 18.
  PUBMED    22279533
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(1)"; pages: "e29266. Epub 2012 Jan 18"
COMMENT     Sequence Label: pRiboS EcoHind
FEATURES             Location/Qualifiers
     source          1..2504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      403..449
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(599..618)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             610..1425
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(683..702)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1756..2344
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2245..2264
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcggccgcgg taccagatct ttaaatctag aggtgaccac aacgcgcccg ctttgatcgg
       61 ggacgtctgc ggccgaccat ttacgggtct tgttgtcgtt ggcggtcatg ggccgaacat
      121 actcacccgg atcggagggc cgaggacaag gtcgaacgag gggcatgacc cggtgcgggg
      181 cttcttgcac tcggcatagg cgagtgctaa gaataacgtt ggcactcgcg accgggatac
      241 gactcactat aggtaccggt gataccagca tcgtcttgat gcccttggca gcaccctgct
      301 aaggaggcaa caagatggcc aagacaattg cggatccagc tgcagaattc aagcttatcg
      361 atgtcgacgt agttaactag cgtacgatcg actgccaggc atcaaataaa acgaaaggct
      421 cagtcgaaag actgggcctt tcgttttatg ccatcatggc cgcggtgatc agctagccac
      481 ctgacgtcgg ggggggggga aagccacgtt gtgtctcaaa atctctgatg ttacattgca
      541 caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca
      601 aggggtgtta tgagccatat tcaacgggaa acgtcttgct cgaggccgcg attaaattcc
      661 aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt
      721 gcgacaatct atcgcttgta tgggaagccc catgcgccag agttgtttct gaaacatggc
      781 aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa
      841 tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc
      901 accactgcga tccccgggaa aacagcattc caggtattag aagaatatcc tgattcaggt
      961 gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt
     1021 aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat
     1081 aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa
     1141 gtctggaaag aaatgcataa tcttttgcca ttctcaccgg attcagtcgt cactcatggt
     1201 gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt
     1261 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt
     1321 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat
     1381 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat
     1441 tggttgtaac actggcagag cattacgctg acttgacggg acggcggctt tgttgaataa
     1501 atcgaacttt tgctgagttg aaggatcaga tcacgcatct tcccgacaac gcagaccgtt
     1561 ccgtggcaaa gcaaaagttc aaaatcacca actggtccac ctacaacaaa gctctcacca
     1621 accgtggctc cctcactttc tggctggatg atggggcgat tcaggcctgg tatgagtcag
     1681 caacaccttc ttcacgaggc agacctcact agttccatga gcgtcagacc ccgtagaaaa
     1741 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1801 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1861 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     1921 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1981 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     2041 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     2101 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     2161 cacgcttccc gaggggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     2221 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     2281 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     2341 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2401 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     2461 agctgatacc gctcgccgca gccgaacgac cgagcgcaac gcgt
//
