LOCUS       sequence-450983-       11435 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Over-express EGFP-IRF3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11435)
  AUTHORS   Gentili M, Lahaye X, Nadalin F, Nader GPF, Puig Lombardi E, Herve S,
            De Silva NS, Rookhuizen DC, Zueva E, Goudot C, Maurin M, Bochnakian 
            A, Amigorena S, Piel M, Fachinetti D, Londono-Vallejo A, Manel N
  TITLE     The N-Terminal Domain of cGAS Determines Preferential Association 
            with Centromeric DNA and Innate Immune Activation in the Nucleus.
  JOURNAL   Cell Rep. 2019 Feb 26;26(9):2377-2393.e13. doi: 
            10.1016/j.celrep.2019.01.105.
  PUBMED    30811988
REFERENCE   2  (bases 1 to 11435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.01"; journalName: "Cell Rep"; date: 
            "2019-02-26- 26"; volume: "26"; issue: "9"; pages: "2377-2393.e13"
COMMENT     Sequence Label: pTRIP-GFP-IRF3
FEATURES             Location/Qualifiers
     source          1..11435
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    380..514
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(417..436)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     471..490
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             682..1315
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1362..1487
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1980..2213
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2398..2442
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2735..2852
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2924..3135
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      3150..3159
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3156..3158,3159..3161,3162..3872)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3156..3158,3159..3161,3162..3872)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3201..3222)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3462..3481)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3809..3830
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5536..5716
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7244..7263)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7477..7496)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7596..7618
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7656..7674)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7742..7846
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7847..7915,7916..8707)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8065..8084)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8878..9466
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9367..9386
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(9707..9727)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9712..10041
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9892..10027
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11175..11240
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11370..11390
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac aaaggaaaaa
       61 gctgcactgc tatacaagaa aattatggaa aaatattctg taacctttat aagtaggcat
      121 aacagttata atcataacat actgtttttt cttactccac acaggcatag agtgtctgct
      181 attaataact atgctcaaaa attgtgtacc tttagctttt taatttgtaa aggggttaat
      241 aaggaatatt tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt
      301 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
      361 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
      421 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
      481 caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa
      541 tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt
      601 actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat
      661 atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc
      721 ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag
      781 ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
      841 cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc
      901 tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag
      961 catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag
     1021 cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg
     1081 gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct
     1141 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     1201 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     1261 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1321 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1381 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1441 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1501 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1561 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1621 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1681 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1741 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1801 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1861 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1921 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1981 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     2041 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     2101 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     2161 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     2221 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     2281 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2341 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2401 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2461 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2521 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2581 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2641 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
     2701 cgccgaattc acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     2761 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     2821 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     2881 cactttggct gatacgcgtc gtgaggctcc gatgcccgtc agtgggcaga gcgcacatcg
     2941 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg
     3001 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
     3061 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
     3121 gccgccagaa cacaggatcc ccaccggtcg ccaccatggt gagcaagggc gaggagctgt
     3181 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     3241 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     3301 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
     3361 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     3421 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     3481 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     3541 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     3601 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     3661 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     3721 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
     3781 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     3841 ggatcactct cggcatggac gagctgtaca agtccggact cagatccatg ggaaccccaa
     3901 agccacggat cctgccctgg ctggtgtcgc agctggacct ggggcaactg gagggcgtgg
     3961 cctgggtgaa caagagccgc acgcgcttcc gcatcccttg gaagcacggc ctacggcagg
     4021 atgcacagca ggaggatttc ggaatcttcc aggcctgggc cgaggccact ggtgcatatg
     4081 ttcccgggag ggataagcca gacctgccaa cctggaagag gaatttccgc tctgccctca
     4141 accgcaaaga agggttgcgt ttagcagagg accggagcaa ggaccctcac gacccacata
     4201 aaatctacga gtttgtgaac tcaggagttg gggacttttc ccagccagac acctctccgg
     4261 acaccaatgg tggaggcagt acttctgata cccaggaaga cattctggat gagttactgg
     4321 gtaacatggt gttggcccca ctcccagatc cgggaccccc aagcctggct gtagcccctg
     4381 agccctgccc tcagcccctg cggagcccca gcttggacaa tcccactccc ttcccaaacc
     4441 tggggccctc tgagaaccca ctgaagcggc tgttggtgcc gggggaagag tgggagttcg
     4501 aggtgacagc cttctaccgg ggccgccaag tcttccagca gaccatctcc tgcccggagg
     4561 gcctgcggct ggtggggtcc gaagtgggag acaggacgct gcctggatgg ccagtcacac
     4621 tgccagaccc tggcatgtcc ctgacagaca ggggagtgat gagctacgtg aggcatgtgc
     4681 tgagctgcct gggtggggga ctggctctct ggcgggccgg gcagtggctc tgggcccagc
     4741 ggctggggca ctgccacaca tactgggcag tgagcgagga gctgctcccc aacagcgggc
     4801 atgggcctga tggcgaggtc cccaaggaca aggaaggagg cgtgtttgac ctggggccct
     4861 tcattgtaga tctgattacc ttcacggaag gaagcggacg ctcaccacgc tatgccctct
     4921 ggttctgtgt gggggagtca tggccccagg accagccgtg gaccaagagg ctcgtgatgg
     4981 tcaaggttgt gcccacgtgc ctcagggcct tggtagaaat ggcccgggta gggggtgcct
     5041 cctccctgga gaatactgtg gacctgcaca tttccaacag ccacccactc tccctcacct
     5101 ccgaccagta caaggcctac ctgcaggact tggtggaggg catggatttc cagggccctg
     5161 gggagagctg agccctcgct cctcatggtg tgcctccaac ccccctgttc cccaccacct
     5221 caaccaataa actggttcct gctatgaaaa aaaaaaaaaa aaaaaaactc gagacctaga
     5281 aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct
     5341 agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc
     5401 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     5461 agggctaatt cactcccaac gaagacaaaa tcgtcgagag atgctgcata taagcagctg
     5521 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     5581 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     5641 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     5701 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     5761 aagaaatgaa tatcagagag tgagaggcct tgacattata atagatttag caggaattga
     5821 actaggagtg gagcacacag gcaaagctgc agaagtactt ggaagaagcc accagagata
     5881 ctcacgattc tgcacatacc tggctaatcc cagatcctaa ggattacatt aagtttacta
     5941 acatttatat aatgatttat agtttaaagt ataaacttat ctaatttact attctgacag
     6001 atattaatta atcctcaaat atcataagag atgattacta ttatccccat ttaacacaag
     6061 aggaaactga gagggaaaga tgttgaagta attttcccac aattacagca tccgttagtt
     6121 acgactctat gatcttctga cacaaattcc atttactcct caccctatga ctcagtcgaa
     6181 tatatcaaag ttatggacat tatgctaagt aacaaattac ccttttatat agtaaatact
     6241 gagtagattg agagaagaaa ttgtttgcaa acctgaatag cttcaagaag aagagaagtg
     6301 aggataagaa taacagttgt catttaacaa gttttaacaa gtaacttggt tagaaaggga
     6361 ttcaaatgca taaagcaagg gataaatttt tctggcaaca agactataca atataacctt
     6421 aaatatgact tcaaataatt gttggaactt gataaaacta attaaatatt attgaagatt
     6481 atcaatatta taaatgtaat ttacttttaa aaagggaaca tagaaatgtg tatcattaga
     6541 gtagaaaaca atccttatta tcacaatttg tcaaaacaag tttgttatta acacaagtag
     6601 aatactgcat tcaattaagt tgactgcaga ttttgtgttt tgttaaaatt agaaagagat
     6661 aacaacaatt tgaattattg aaagtaacat gtaaatagtt ctacatacgt tcttttgaca
     6721 tcttgttcaa tcattgatcg aagttcttta tcttggaaga atttgttcca aagactctga
     6781 aataaggaaa acaatctatt atatagtctc acacctttgt tttactttta gtgatttcaa
     6841 tttaataatg taaatggtta aaatttattc ttctctgaga tcatttcaca ttgcagatag
     6901 aaaacctgag actggggtaa tttttattaa aatctaattt aatctcagaa acacatcttt
     6961 attctaacat caatttttcc agtttgatat tatcatataa agtcagcctt cctcatctgc
     7021 aggttccaca acaaaaatcc aaccaactgt ggatcaaaaa tattgggaaa aaattaaaaa
     7081 tagcaataca acaataaaaa aatacaaatc agaaaaacag cacagtataa caactttatt
     7141 tagcatttac aatctattag gtattataag taatctagcc agatcctcta cgccggacgc
     7201 atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc
     7261 accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt
     7321 atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc
     7381 cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag
     7441 tcgcataagg gagagcgtcg aatggtgcac tctcagtaca atctgctctg atgccgcata
     7501 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     7561 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     7621 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     7681 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     7741 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     7801 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     7861 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     7921 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     7981 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     8041 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     8101 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     8161 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     8221 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     8281 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     8341 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     8401 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     8461 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     8521 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     8581 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     8641 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     8701 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     8761 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     8821 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     8881 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     8941 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     9001 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     9061 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     9121 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     9181 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     9241 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     9301 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     9361 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     9421 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     9481 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     9541 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     9601 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     9661 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctgtggaa tgtgtgtcag
     9721 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     9781 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     9841 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     9901 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     9961 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
    10021 ggaggcctag gcttttgcaa aaagcttgga cacaagacag gcttgcgaga tatgtttgag
    10081 aataccactt tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg actcatgtga
    10141 aatactggtt tttagtgcgc cagatctcta taatctcgcg caacctattt tcccctcgaa
    10201 cactttttaa gccgtagata aacaggctgg gacacttcac atgagcgaaa aatacatcgt
    10261 cacctgggac atgttgcaga tccatgcacg taaactcgca agccgactga tgccttctga
    10321 acaatggaaa ggcattattg ccgtaagccg tggcggtctg taccgggtgc gttactggcg
    10381 cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt ccagctacga tcacgacaac
    10441 cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt
    10501 gatgacctgg tggataccgg tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg
    10561 cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt
    10621 gatatcccgc aagatacctg gattgaacag ccgtgggata tgggcgtcgt attcgtcccg
    10681 ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt tctttttaac
    10741 ttcaggcggg ttacaatagt ttccagtaag tattctggag gctgcatcca tgacacaggc
    10801 aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct cgacgctagt
    10861 ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat
    10921 ccctcactgg tatcgcatga ttaaccgtct gatgtggatc tggcgcggca ttgacccacg
    10981 cgaaatcctc gacgtccagg cacgtattgt gatgagcgat gccgaacgta ccgacgatga
    11041 tttatacgat acggtgattg gctaccgtgg cggcaactgg atttatgagt gggccccgga
    11101 tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga
    11161 tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc
    11221 taattgtttg tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga
    11281 atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg
    11341 ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca
    11401 aggactttcc ttcagaattg ctaagttttt tgagt
//
