LOCUS       sequence-451004-        9049 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP-tagged BRD protein, which can be used to be expressed in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9049)
  AUTHORS   Gong F, Chiu LY, Cox B, Aymard F, Clouaire T, Leung JW, Cammarata M,
            Perez M, Agarwal P, Brodbelt JS, Legube G, Miller KM
  TITLE     Screen identifies bromodomain protein ZMYND8 in chromatin 
            recognition of transcription-associated DNA damage that promotes 
            homologous recombination.
  JOURNAL   Genes Dev. 2015 Jan 15;29(2):197-211. doi: 10.1101/gad.252189.114.
  PUBMED    25593309
REFERENCE   2  (bases 1 to 9049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.252189"; journalName: "Genes Dev"; date: "2015-01-15- 
            15"; volume: "29"; issue: "2"; pages: "197-211"
COMMENT     Sequence Label: GFP-BRD1
FEATURES             Location/Qualifiers
     source          1..9049
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        105..484
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        485..688
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     639..659
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     685..709
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(813..815,816..818,819..1529)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(858..879)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1119..1138)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1466..1487
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    1545..1569
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1701..1712
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(4744..4768)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4776..4817
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(4857..4876)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4901..4949
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5151..5579
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5238..5257)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5448..5469
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5588..5608)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5593..5922
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5773..5908
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5835..5854
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5970..6017
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6036..6434
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    6592..6713
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6629..6648)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6683..6702
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6762..6778)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6762..6778)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6775..6797)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6786..6802
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6810..6816,6817..6834,6835..6840))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6855..6876
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6993..7010)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7164..7752)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7244..7263)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7923..8714,8715..8783))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8546..8565
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8784..8888)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8963..8982)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
       61 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac
      121 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
      181 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      241 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      301 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      361 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      421 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      481 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      541 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      601 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      661 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      721 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct
      781 agaggatccc taccggtgat atcctcgaga ccatggtgag caagggcgag gagctgttca
      841 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
      901 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      961 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcacc tacggcgtgc
     1021 agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     1081 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     1141 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     1201 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     1261 aggtctatat caccgccgac aagcagaaga acggcatcaa ggtgaacttc aagacccgcc
     1321 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     1381 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     1441 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     1501 tcactctcgg catggacgag ctgtacaagg gctcgagccc atcaacaagt ttgtacaaaa
     1561 aagttggcac catgaggagg aaaggacgat gtcatcgagg ctctgcagcg aggcatcctt
     1621 cttccccatg cagtgttaaa cactccccta cgcgagaaac gctgacctac gctcaagctc
     1681 aaaggatggt agagatagaa attgaagggc gcttgcacag gatcagtatt tttgatcccc
     1741 tggagatcat attggaagat gacctcactg ctcaagagat gagtgagtgc aacagcaaca
     1801 aggaaaacag cgagcggcct cctgtctgct taagaactaa gcgtcacaaa aacaacagag
     1861 tcaaaaagaa aaacgaggcc ctccccagcg cccacggcac gccggcctcg gccagtgccc
     1921 tcccggagcc caaggtgcgc atcgtggagt acagccctcc gtccgccccc aggaggcctc
     1981 ctgtgtacta caagttcatc gagaagtcgg ccgaggaact ggacaacgag gtggagtatg
     2041 acatggacga ggaggactat gcctggctgg agatcgtcaa tgagaagcgc aagggcgact
     2101 gcgtccccgc cgtgtcgcag agcatgtttg agttcctgat ggaccgcttc gagaaggagt
     2161 cgcactgcga gaaccagaag cagggcgagc agcagtctct gatcgacgag gacgccgtgt
     2221 gctgcatctg catggacggg gagtgtcaga acagcaacgt gatcctcttc tgcgacatgt
     2281 gcaacctggc cgtgcaccag gagtgctacg gggtgcccta catccccgag ggccagtggc
     2341 tctgccgcca ctgcctgcag tcgcgggccc ggcccgccga ctgtgtgctg tgccccaaca
     2401 agggtggtgc cttcaaaaag acagatgacg accgctgggg tcacgtggtg tgtgccctgt
     2461 ggatcccaga ggtcggcttt gccaacacgg tgttcatcga gcccatcgat ggggtgagga
     2521 acatccctcc agcccggtgg aaactgacat gctacctctg taagcagaag ggcgtgggtg
     2581 cctgcatcca gtgccacaaa gcaaactgct acacagcatt ccatgtgacg tgtgcccaga
     2641 aggctggcct gtacatgaaa atggagcccg tgaaggaact gactggcggt ggcaccacct
     2701 tctccgtcag aaagaccgct tactgtgatg tccacacgcc tccaggctgc acccggaggc
     2761 ctctgaatat ttacggggat gtcgaaatga aaaatggcgt ctgtcgaaaa gagagctcgg
     2821 ttaaaacggt caggtccaca tccaaggtca ggaagaaggc aaaaaaggct aagaaagctc
     2881 tggctgagcc ctgcgcggtc ctgccgaccg tgtgcgctcc ttatattccc ccgcagaggt
     2941 taaataggat tgcgaatcag gtggccattc agcggaagaa gcagtttgtg gagcgagccc
     3001 acagctactg gctgctcaag cggctgtcca ggaacggggc ccccctgctg cggcggctgc
     3061 agtccagcct gcagtctcag cgaagctcac agcagagaga aaatgatgag gagatgaagg
     3121 ctgccaaaga gaagctgaag tactggcagc ggctgcggca cgacctggag cgcgctcgcc
     3181 tgctgatcga gctgctgcgc aagcgggaga agctcaagcg tgagcaggtg aaggtggagc
     3241 aggtcgccat ggagctgcgg ctgaccccgc tgacggtgct gctgcgctca gtgctggacc
     3301 agctgcaaga caaggacccc gccaggatat ttgcgcagcc cgtgagtctg aaggaggtac
     3361 cagattattt ggatcacatt aaacatccca tggactttgc cacaatgagg aaacggttag
     3421 aagctcaagg gtataaaaac ctccatgagt ttgaggagga ttttgatctc attatagata
     3481 actgcatgaa gtacaatgcc agggacaccg tgttctatag agccgcggtg aggctgcgcg
     3541 atcagggagg tgttgttctg aggcaggccc ggcgcgaggt ggacagcatc ggcttggaag
     3601 aggcctcggg gatgcacctg cctgagcggc ctgctgcggc accgcggcgg cctttctcct
     3661 gggaagacgt ggacaggttg ctggaccccg ccaacagagc ccacctgggc ctggaggagc
     3721 agctgagaga gctgctggac atgctcgacc tcacctgcgc tatgaagtcc agcggctccc
     3781 ggagcaagcg ggcaaagctg ctcaaaaagg aaattgccct tctccgaaac aagctgagcc
     3841 agcagcacag ccagcccctg cccacggggc caggcttgga aggcttcgaa gaggacggag
     3901 ctgcgctggg gccggaggcg ggcgaggaag tccttccgag gttggagact cttctgcagc
     3961 caaggaaaag gtcgcggagc acatgcggag actccgaggt ggaggaggag tccccaggaa
     4021 agcgcctgga cgcaggtctc accaacggct ttgggggtgc gaggagcgag caggagccgg
     4081 gcggcggcct ggggaggaag gccacacccc gacgacgctg tgcctccgag tccagcatct
     4141 cctccagcaa cagcccgctc tgcgactcga gctttaatgc gcccaaatgt gggcggggca
     4201 aaccggctct tgtgcgacgg cacacgctgg aggaccgcag tgagctgatc tcctgcatcg
     4261 agaatgggaa ctacgccaag gcggccagga tcgcagccga agtcggccag agcagcatgt
     4321 ggatctccac tgatgccgcc gcctcggtgc tggagcctct gaaggtggtg tgggccaagt
     4381 gcagcggcta cccctcctac ccggcactga tcatcgaccc caagatgccc cgtgtgcctg
     4441 gccaccacaa cggcgtcacc atcccggccc cacccctgga cgtgctgaag attggggagc
     4501 acatgcagac caagtctgat gagaagctgt tcctcgttct cttttttgat aataagagaa
     4561 gttggcagtg gcttcctaag tccaaaatgg ttccccttgg tattgacgaa actatagaca
     4621 agttaaagat gatggaaggg aggaattcca gcatccggaa ggccgtgcgg atcgcttttg
     4681 accgcgccat gaaccacctg agccgcgtcc acggggagcc gaccagcgac ctcagtgaca
     4741 ttgacccaac tttcttgtac aaagtggttg ataacgggaa gcctatccct aaccctctcc
     4801 tcggtctcga ttctacgcgt accggttagt aatgagttta aacgggggag gctaactgaa
     4861 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat
     4921 aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca
     4981 ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc
     5041 cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc
     5101 aggccctgcc atagcagatc tgcgcagctg gggctctagg gggtatcccc acgcgccctg
     5161 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     5221 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     5281 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     5341 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     5401 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     5461 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     5521 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
     5581 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     5641 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     5701 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     5761 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     5821 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
     5881 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
     5941 atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg
     6001 gcatagtata atacgacaag gtgaggaact aaaccatggc caagcctttg tctcaagaag
     6061 aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact
     6121 acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat
     6181 atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg
     6241 cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc
     6301 cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa gccatagtga
     6361 aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg
     6421 tgtgggaggg ctaagcactt cgtggccgag gagcaggact gacacgtgct acgagatttc
     6481 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     6541 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     6601 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     6661 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     6721 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     6781 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     6841 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     6901 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     6961 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     7021 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     7081 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     7141 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     7201 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     7261 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     7321 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     7381 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     7441 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     7501 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     7561 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     7621 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     7681 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     7741 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     7801 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     7861 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     7921 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     7981 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     8041 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     8101 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     8161 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     8221 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     8281 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     8341 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     8401 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     8461 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     8521 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     8581 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     8641 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     8701 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     8761 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     8821 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     8881 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg
     8941 atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
     9001 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaat
//
