LOCUS       sequence-451006-        4470 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC34A1_STOP
FEATURES             Location/Qualifiers
     source          1..4470
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2595..2694)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2711..2730)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2712..2730)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2735..2751)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2735..2751)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2853..2872)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2864..3673
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2931..2950)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3820..4408
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4309..4328
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgctga gctacggaga gaggctggga tccccagccg tgtctcccct
      721 gcctgtgcgg ggaggacacg tgatgagagg aaccgccttt gcctacgtgc catccccaca
      781 ggtgctgcac aggatcccag gcacaagcgc ctatgcattc ccatccctgg gaccagtggc
      841 cctggcagag cacacctgcc cttgtggaga ggtgctggag aggcacgagc ctctgccagc
      901 aaagctggcc ctggaggagg agcagaagcc cgagagcagg ctggtgccta agctgaggca
      961 ggcaggagca atgctgctga aggtgcctct gatgctgacc tttctgtatc tgttcgtgtg
     1021 ctccctggac atgctgagct ccgcctttca gctggcagga ggcaaggtgg caggcgacat
     1081 cttcaaggat aacgccatcc tgtctaatcc tgtggcagga ctggtggtgg gaatcctggt
     1141 gacagtgctg gtgcagtcta gctccacctc cacatctatc atcgtgtcca tggtgtctag
     1201 cggcctgctg gaggtgtcct ctgccatccc aatcatcatg ggctctaaca tcggcaccag
     1261 cgtgaccaat acaatcgtgg ccctgatgca ggcaggcgac aggaccgatt ttcggagagc
     1321 cttcgccgga gcaacagtgc acgattgttt caattggctg tctgtgctgg tgctgctgcc
     1381 actggaggca gcaaccggat acctgcacca catcacaagg ctggtggtgg ccagctttaa
     1441 catccacgga ggaagggacg caccagatct gctgaagatc atcaccgagc ctttcacaaa
     1501 gctgatcatc cagctggacg agagcgtgat cacctccatc gccacaggcg atgagtccct
     1561 gaggaatcac tctctgatcc agatctggtg ccacccagac agcctgcagg cacccaccag
     1621 catgtccagg gcagaggcaa acagctccca gaccctgggc aatgccacaa tggagaagtg
     1681 taaccacatc tttgtggaca caggcctgcc tgatctggca gtgggactga tcctgctggc
     1741 aggatctctg gtgctgctgt gcacctgtct gatcctgctg gtgaagatgc tgaatagcct
     1801 gctgaagggc caggtggcca aagtgatcca gaaagtgatc aacacagatt ttcccgcccc
     1861 tttcacctgg gtgacaggct acttcgccat ggtggtgggc gccagcatga cctttgtggt
     1921 gcagtctagc tccgtgttca cctccgccat cacaccactg atcggactgg gcgtgatctc
     1981 catcgagagg gcatatccac tgaccctggg atctaatatc ggcaccacaa ccacagcaat
     2041 cctggccgcc ctggcaagcc caagagagaa gctgtctagc gcctttcaga tcgccctgtg
     2101 ccacttcttt ttcaacatct ccggcatcct gctgtggtat ccagtgccct gtaccaggct
     2161 gccaatcagg atggcaaagg ccctgggcaa gaggacagcc aagtaccgct ggttcgccgt
     2221 gctgtatctg ctggtgtgct ttctgctgct gccttctctg gtgttcggca tcagcatggc
     2281 aggatggcag gtcatggtgg gagtgggcac accatttggc gccctgctgg ccttcgtggt
     2341 gctgatcaat gtgctgcagt ctcggagccc tggacacctg ccaaagtggc tgcagacctg
     2401 ggactttctg cctcggtgga tgcactccct gaagccactg gatcacctga tcaccagagc
     2461 cacactgtgc tgtgcaaggc ctgagccaag atccccacct ctgccaccca gagtgttcct
     2521 ggaggagctg cctccagcaa ccccatctcc taggctggcc ctgccagcac accacaacgc
     2581 aacaagactg taagacccag ctttcttgta caaagttggc attataagaa agcattgctt
     2641 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc
     2701 agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg
     2761 gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac
     2821 aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg
     2881 ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg
     2941 ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga
     3001 tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga
     3061 gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat
     3121 ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca
     3181 ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct
     3241 gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg
     3301 tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga
     3361 cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt
     3421 ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga
     3481 ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga
     3541 tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt
     3601 tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga
     3661 tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac
     3721 ttgacgggac ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca
     3781 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     3841 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     3901 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     3961 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     4021 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     4081 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     4141 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     4201 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     4261 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     4321 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     4381 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     4441 ggccttttgc tggccttttg ctcacatgtt
//
