LOCUS       sequence-451027-       15491 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral expression of human POLQ -DY2230AA mutant (a polymerase 
            domain mutant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15491)
  AUTHORS   Yousefzadeh MJ, Wyatt DW, Takata K, Mu Y, Hensley SC, Tomida J, 
            Bylund GO, Doublie S, Johansson E, Ramsden DA, McBride KM, Wood RD
  TITLE     Mechanism of suppression of chromosomal instability by DNA 
            polymerase POLQ.
  JOURNAL   PLoS Genet. 2014 Oct 2;10(10):e1004654. doi: 
            10.1371/journal.pgen.1004654. eCollection 2014 Oct.
  PUBMED    25275444
REFERENCE   2  (bases 1 to 15491)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2014-10-2"; volume: "10(10):e1004654. doi"; pages: "
            10.1371/journal.pgen.1004654. eCollection 2014 Oct"
COMMENT     Sequence Label: pCDH-EF1-FHC-POLQ-DY2230AA
FEATURES             Location/Qualifiers
     source          1..15491
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2082..2350
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(2363..2574)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_feature    2617..2733
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(3000..3044)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3229..3462
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3955..4080
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4127..4307
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(4308..4534)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(4549..4566)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4549..4565)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4558..4580)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4774..4793)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4893..4915
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4953..4971)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5039..5143
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5144..5212,5213..6004)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5362..5381)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6175..6763
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6917..6934
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7051..7072
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7087..7092,7093..7110,7111..7117)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7125..7141
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7130..7152
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7149..7165
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7149..7165
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      7198..7333
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7373..7494
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7384..7403)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7438..7457
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             7566..7799
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    7873..8461
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             7979..7990
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     8388..8408
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8468..9067)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8551..8571)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     9048..9067
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     misc_feature    9076..9648
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(9235..9254)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     9464..9481
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(9659..9685)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             complement(9659..9685)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(9701..9724)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cataaacaga aaaatcgata ccaatcaata gtagtccaat cctcaaacat aggtgtaacc
       61 agatagagaa tatgaagatc attctctaaa acaaagccct tcattgctct ttgcaggtca
      121 gcaaaaatat ctaaagtatc agctggagaa agtgaagaag aaagagtggc cgaaccaaga
      181 tgtgttggat gatacacctt tccttctgtt ccatcactgg cttctgtact ctggatgaat
      241 tcattttcta gtagccacat cacacaggcc tcaatcgctc caagctgaac agactcttga
      301 tttctctgaa ttccttgctt cccttctttc atacttgcag ccaaaaatgt gcaggcagca
      361 taagtatgca tatcttgtga tgtacttgcc actccaccaa ctattatctc cagaatagct
      421 cgtatcatgc tgccagttac ttcttctcct tctcgtcttt gcagacagct gcgaacaggc
      481 tttagagaac cctgaaggag agctatgcct tttgatttct cagagttctt acaaattaag
      541 atactctcgc ctactgtgtc cactcctttc ctgccagcac gaccaaccat ctgcttataa
      601 gtaagaatat ctagaggtcg accaccaaaa ataggggttc gaataatcac acgacgtgca
      661 ggtaaattca ccccagaaga aagagtagaa gttgccgcca agactcgaat gagaccttga
      721 cgaaaggctc cttcaatgat atccctctcc tcaaaagtaa gacctgcatg atgaaatgct
      781 actccccatg gtacagtttt ctgtaataca gagtccagtc ctgaaggcaa ccgtcttaac
      841 tgatccatca cttccaggag ttctttttgt tccagaatta ctggtgggca ttcagagggt
      901 ttcaccaatc cctcagcttg atgatgtaga ttataaaact ctcgagcaat gatatctgcc
      961 agcttctcac accatttctt tgatggacaa aaaagtaata ctgaatggtt atcacaaatc
     1021 gtctcataac ataagctaac aacatggtcc tcatctccct tcacttgaag catgggctca
     1081 aattccctca caagtttcat tgaagagtca tatatggaat ttccaacttt tactgactcc
     1141 aaaagcggta cagggcgaaa gtcggtatgg tagagttcag cattcaacca ggaagccaca
     1201 agctccaaat taggaagggt agcactcatg ccaacgattt gcacagcatt agacagagaa
     1261 ctggctagat ctgcctgaca agatgctgat ttccgagtaa tatagcaaat cttggtcagc
     1321 aaaagttcca gcagataccc tcggtgagag tctcccagca tatgtaattc atccacaacc
     1381 accattccta acagatccat cttattttcc tctatgaggc gattgatcag accattggct
     1441 ctctcaattg tgcagactgc aatatccaat gaagagaaat gccttgatgg agaggtgctg
     1501 cccatataac cgtctacttt tattcctact tcctgaaaca gactctggag gtagtatttc
     1561 ttctctttag ccacagaaac aaagggaaga ataaacaaag ctttcttccg catttccaaa
     1621 acccgcttca aaataagtaa ttctgccaca agagtcttcc cagcacttgt aggagctgaa
     1681 taaactaaat tctttccttc caggacttgt ccaagcaaaa ggcactctgc ctgccattca
     1741 aacatctttt ttacaccaaa actgtggtat ttttccagaa ctgctttagg aagtccccag
     1801 tttgccaata gtagcttgtc tatttcgtag tcaggaactg taggcttgca ttctcctgca
     1861 gctgcggcct tcaggcagcg accaaggccg ggcggcgggc tcagcacgga cccggagagg
     1921 aactgggggc tggcactgct gtcaccgccg cttcccgaga acgaatctga gcctgattct
     1981 gaacgccgcc gtttcccact ccgacgcaga agattcatgg tgctaggatc cgatttaaat
     2041 tcgaattcgc tagctctaga gtaggcgccg gtcacagctt ggatctgtaa cggcgcagaa
     2101 cagaaaacga aacaaagacg tagagttgag caagcagggt caggcaaagc gtggagagcc
     2161 ggctgagtct aggtaggctc caagggagcg ccggacaaag gcccggtctc gacctgagct
     2221 ttaaacttac ctagacggcg gacgcagttc aggaggcacc acaggcggga ggcggcagaa
     2281 cgcgactcaa ccggcgtgga tggcggcctc aggtagggcg gcgggcgcgt gaaggagaga
     2341 tgcgagcccc tcgaagcttc agctgtgttc tggcggcaaa cccgttgcga aaaagaacgt
     2401 tcacggcgac tactgcactt atatacggtt ctcccccacc ctcgggaaaa aggcggagcc
     2461 agtacacgac atcactttcc cagtttaccc cgcgccacct tctctaggca cccgttcaat
     2521 tgccgacccc tccccccaac ttctcgggga ctgtgggcga tgtgcgctct gcccactgac
     2581 gggcaccgga gcgatcgcag atccactagt atcgataaaa ttttgaattt tgtaatttgt
     2641 ttttgtaatt ctttagtttg tatgtctgtt gctattatgt ctactattct ttcccctgca
     2701 ctgtaccccc caatcccccc ttttctttta aaagttaacc gataccgtcg agatccgttc
     2761 actaatcgaa tggatctgtc tctgtctctc tctccacctt cttcttctat tccttcgggc
     2821 ctgtcgggtc ccctcggggt tgggaggtgg gtctgaaacg ataatggtga atatccctgc
     2881 ctaactctat tcactataga aagtacagca aaaactattc ttaaacctac caagcctcct
     2941 actatcatta tgaataattt tatataccac agccaatttg ttatgttaaa ccaattccac
     3001 aaacttgccc atttatctaa ttccaataat tcttgttcat tcttttcttg ctggttttgc
     3061 gattcttcaa ttaaggagtg tattaagctt gtgtaattgt taatttctct gtcccactcc
     3121 atccaggtcg tgtgattcca aatctgttcc agagatttat tactccaact agcattccaa
     3181 ggcacagcag tggtgcaaat gagttttcca gagcaacccc aaatccccag gagctgttga
     3241 tcctttaggt atctttccac agccaggatt cttgcctgga gctgcttgat gccccagact
     3301 gtgagttgca acagatgctg ttgcgcctca atagccctca gcaaattgtt ctgctgctgc
     3361 actataccag acaataattg tctggcctgt accgtcagcg tcattgacgc tgcgcccata
     3421 gtgcttcctg ctgctcccaa gaacccaagg aacaaagctc ctattcccac tgctcttttt
     3481 tctctctgca ccactcttct ctttgccttg gtgggtgcta ctcctaatgg ttcaattttt
     3541 actactttat atttatataa ttcacttctc caattgtccc tcatatctcc tcctccaggt
     3601 ctgaagatca gtggccgctt gctgtgcggt ggtcttactt ttgttttgct cttcctctat
     3661 cttgtctaaa gcttccttgg tgtcttttat ctctatcctt tgatgcacac aatagagggt
     3721 tgctactgta ttatataatg atctaagttc ttctgatcct gtctgaaggg atggttgtag
     3781 ctgtcccagt atttgtctac agccttctga tgtttctaac aggccaggat taactgcgaa
     3841 tcgttctagc tccctgcttg cccatactat atgttttaat ttatattttt tctttccccc
     3901 tggccttaac cgaatttttt cccatcgcga tctaattctc ccccgcttaa tactgacgct
     3961 ctcgcaccca tctctctcct tctagcctcc gctagtcaaa atttttggcg tactcaccag
     4021 tcgccgcccc tcgcctcttg ccgtgcgcgc ttcagcaagc cgagtcctgc gtcgagagag
     4081 ctcctctggt ttccctttcg ctttcaagtc cctgttcggg cgccactgct agagattttc
     4141 cacactgact aaaagggtct gagggatctc tagttaccag agtcacacaa cagacgggca
     4201 cacactactt gaagcactca aggcaagctt tattgaggct taagcagtgg gttccctagt
     4261 tagccagaga gctcccaggc tcagatctgg tctaaccaga gagacccgtt tatgtatcga
     4321 gctaggcact taaatacaat atctctgcaa tgcggcaatt cagtggttcg tccaatccat
     4381 gtcagacccg tctgttgcct tcctaataag gcacgatcgt accaccttac ttccaccaat
     4441 cggcatgcac ggtgcttttt ctctccttgt aaggcatgtt gctaactcat cgttaccatg
     4501 ttgcaagact acaagagtat tgcataagac tacacgcgtc agcttggcac tggccgtcgt
     4561 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     4621 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     4681 gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg
     4741 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     4801 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     4861 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     4921 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     4981 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     5041 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     5101 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     5161 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     5221 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     5281 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     5341 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     5401 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     5461 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     5521 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     5581 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     5641 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     5701 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     5761 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     5821 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     5881 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     5941 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     6001 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     6061 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     6121 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     6181 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     6241 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     6301 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     6361 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     6421 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     6481 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     6541 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     6601 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     6661 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     6721 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     6781 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     6841 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     6901 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     6961 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     7021 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     7081 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     7141 atttcacaca ggaaacagct atgacatgat tacgaatttg gtctctgcaa aagtctaggc
     7201 ctccaaaaaa gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct
     7261 ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag
     7321 ttaggggcgg gatgggcgga gttaggggcg ggatagctag agccagacat gataagatac
     7381 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     7441 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttcctctc
     7501 actctctgat attcatttct ttgcaagtta taaatactga ataataagat gacatgaact
     7561 actactgcta gagattttcc acactgacta aaagggtctg agggatctct agttaccaga
     7621 gtcacacaac agacgggcac acactacttg aagcactcaa ggcaagcttt attgaggctt
     7681 aagcagtggg ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag
     7741 agacccagta caagcaaaaa gcagatctta tcttcgttgg gagtgaatta gcccttccag
     7801 tccccccttt tcttttaaaa agtggctaag atctacagct gccttgtaag tcattggtct
     7861 taaaggtacc aggcggggag gcggcccaaa gggagatccg actcgtctga gggcgaaggc
     7921 gaagacgcgg aagaggccgc agagccggca gcaggccgcg ggaaggaagg tccgctggat
     7981 tgagggccga agggacgtag cagaaggacg tcccgcgcag aatccaggtg gcaacacagg
     8041 cgagcagcca aggaaaggac gatgatttcc ccgacaacac cacggaattg tcagtgccca
     8101 acagccgagc ccctgtccag cagcgggcaa ggcaggcggc gatgagttcc gccgtggcaa
     8161 tagggagggg gaaagcgaaa gtcccggaaa ggagctgaca ggtggtggca atgccccaac
     8221 cagtgggggt tgcgtcagca aacacagtgc acaccacgcc acgttgcctg acaacgggcc
     8281 acaactcctc ataaagagac agcaaccagg atttatacaa ggaggagaaa atgaaagcca
     8341 tacgggaagc aatagcatga tacaaaggca ttaaagcagc gtatccacat agcgtaaaag
     8401 gagcaacata gttaagaata ccagtcaatc tttcacaaat tttgtaatcc agaggttgat
     8461 tgtcgaccta ggcaccgggc ttgcgggtca tgcaccaggt gcgcggtcct tcgggcacct
     8521 cgacgtcggc ggtgacggtg aagccgagcc gctcgtagaa ggggaggttg cggggcgcgg
     8581 aggtctccag gaaggcgggc accccggcgc gctcggccgc ctccactccg gggagcacga
     8641 cggcgctgcc cagacccttg ccctggtggt cgggcgagac gccgacggtg gccaggaacc
     8701 acgcgggctc cttgggccgg tgcggcgcca ggaggccttc catctgttgc tgcgcggcca
     8761 gccgggaacc gctcaactcg gccatgcgcg ggccgatctc ggcgaacacc gcccccgctt
     8821 cgacgctctc cggcgtggtc cagaccgcca ccgcggcgcc gtcgtccgcg acccacacct
     8881 tgccgatgtc gagcccgacg cgcgtgagga agagttcttg cagctcggtg acccgctcga
     8941 tgtggcggtc cggatcgacg gtgtggcgcg tggcggggta gtcggcgaac gcggcggcga
     9001 gggtgcgtac ggccctgggg acgtcgtcgc gggtggcgag gcgcaccgtg ggcttgtact
     9061 cggtcattcc ggaggtatta tcgtgttttt caaaggaaaa ccacgtcccc gtggttcggg
     9121 gggcctagac gtttttttaa cctcgactaa acacatgtaa agcatgtgca ccgaggcccc
     9181 agatcagatc ccatacaatg gggtaccttc tgggcatcct tcagcccctt gttgaatacg
     9241 cttgaggaga gccatttgac tctttccaca actatccaac tcacaacgtg gcactggggt
     9301 tgtgccgcct ttgcaggtgt atcttataca cgtggctttt ggccgcagag gcacctgtcg
     9361 ccaggtgggg ggttccgctg cctgcaaagg gtcgctacag acgttgtttg tcttcaagaa
     9421 gcttccagag gaactgcttc cttcacgaca ttcaacagac cttgcattcc tttggcgaga
     9481 ggggaaagac ccctaggaat gctcgtcaag aagacagggc caggtttccg ggccctcaca
     9541 ttgccaaaag acggcaatat ggtggaaaat aacatataga caaacgcaca ccggccttat
     9601 tccaagcggc ttcggccagt aacgttaggg gggggggagg gagaggggcg cggccctagg
     9661 cgtagtcggg cacgtcgtag gggtatcctc cagcggccga cttgtcatcg tcgtccttgt
     9721 agtctcctcc agcggccgcc acatcaaagt cctttagctc tccccagctg gcgcctattt
     9781 tcactttcac tttcaatttc acagacagtt ttacagcact ttccatttca ttcttgacaa
     9841 tctgagctac ctgaacaaca tcttcttctg ccacttcata taggagttca tcatggagtt
     9901 gaaggatgaa gaagcctcct ctgattgggc agaacatccc ttgcagtttt ctctttcgtg
     9961 acaatcctgt tcggtcactt tggagcatac cctctcgatg accatgggat ttgaaggttg
    10021 agtggaaggt ctctaattgc ttctgaatgt taactgtggc tattttgaca atatcagctg
    10081 ctgatccttg gactattgtg ttgatagctt gacgctcagc gtgagcttta cgataagggt
    10141 tgttgtcttt gattcctggc aaatatctac gccttcccaa aatggtctga acaaatccgt
    10201 ctcttttaca attcttcact gtctctgtca tgaattgatt aatccctgtg tatctggatt
    10261 tgaaggagtc aatatagcat gcagcatcat tttctttaat gcccatctgc tctcccaaag
    10321 atttagctcc cattccataa atgatcccat agcaaatctg ttttgcctgc tgcctcagat
    10381 catccccaac agactctggc tcaatcatct tccactctgc tgcaatgctc ctgaaaacat
    10441 cagctccagt gtttaacact tgaatgagac gacgatcatg ggataaatga gccaagatcc
    10501 tcagttcaag ctgagaggcg gcagcagcca gtattgaacc acctgggaaa ggcacaaagg
    10561 catgtcgcat gctaattgaa aatggcattc ctctgtctgc agctctctcc tccatctgtg
    10621 cctggcatct aggattcacg ctgaaaccct tcttatattt tcctctgccc atgggaagta
    10681 ggcctttgcc tacagcttga gaaggtgggc tttctcctac tagtgttggc attttgattt
    10741 caaaatctct tggcacattc tgaatatttg gttctgtaaa ggttattcgt cctgtagcag
    10801 tgtgcgactg tgatacagga tagattcttt ccattccaag aaaaggatta agacactttt
    10861 cccgctgaag gggaaagacc actttggtaa tagcattagt gattcttctc cattctaata
    10921 tcaagcctgg taaaggatgt aatgccttta atttatttaa aacgtcctta ctagtgctga
    10981 actgtcttcc cagccttagc ttgcgtccat tgtcaatccc tcttctggta gaacccagag
    11041 ttttcttgct gccttggttt ttcatctctc tatttggggg caacttcaat tccaaaaata
    11101 aaacctcagc gatgtcatct gaactggtga aagaaaaact gtggccagct agttgatagg
    11161 cctgggtctc aattgcatcc agcttggctt gcattatatg tttctgactt tcacattctg
    11221 cagtactaaa gccaattcca tttagttcta gcaaggccaa gcagtactga gagggcattt
    11281 ccaccttacg gaaaacatct tgaaggtttt ccttctgcaa caaagagttg agctgattca
    11341 tagagttgaa gatgagaatg gactccacag atgctctgta tcgcccagaa tgctcactgc
    11401 cagcatttag ccccaggctt tgaatccctt ggctggtctc catcccttct aggagtggaa
    11461 gctcatgagg aagaaaactg gtaactatgc tatgaagagt cggctcctga gaatctggat
    11521 ctagtaacca gcatgccacc ttaggatctt cataactttg ctccaaggag atgccacaag
    11581 aaagaagaag aattttatag ctctggatga agtcatagat gacaacagaa cattctttat
    11641 cagattcctt tcgcaagcaa gattgaaggt accacatcct gtctttcaaa gtcaggcttg
    11701 gatctaaaga aggtggaacc aaactggcac taatttcaga atgcttttgt tccttctgca
    11761 gtgaaaaata ataggcatcc cttccacccc agcatactgc cagtccaacc accaaggtgt
    11821 catcacaacc tttaatggga aatccatcat ctctaatagg aatttcctga ggtgagctag
    11881 cttgcttaaa cctactgcca atagtagcag ttttagaaga tgtcaaactt ctaatctttt
    11941 cacaagccag tgagatggaa aatcgctttt tgcaccgcca ctccttaatg aatgtttgga
    12001 aaagattttg gtcacttgct acatcaatta tggacaaact ttctgaactg cttgaggctg
    12061 gagttaactg taatccatcc tgtgataact gaagtgaaaa gcttgtgtca ctaatagggc
    12121 tgttgtcttt gaacccattt ctactccctg gacttaaatc gtggttttta atatctgaag
    12181 gtgagccaaa taaataactt tcaccaggtt gtaaaacatt attagttttc caagggttta
    12241 caggtgtttc aagaatccct ggaaatgtca gcttagaagc agatgttgga atgggtgtag
    12301 gaggaatgag accattatca tcaactatat tactttcttt acgaggtaag agggatgagg
    12361 tttcatcatg attggcattg ttttctagca tctcaatctt gctttttact tcattattag
    12421 aagaaaatga aattccctgc acttgttttg tctccaagtt tgaaataact tcttgttctt
    12481 catttaactc tgtatttttt ctattcaaac tggaaaagtt tatagtcatt gattttagct
    12541 tttcattttc tagaggactg gatactttat ctaaaatcct ttgcagtcct ggacttagat
    12601 caaatgatgc ccctgaccaa atgaatgaat gattttgcct ggtcccagtt aattttgatt
    12661 tttcagccct ttcatcttga tctcctccat cttgatcacc ttggtggtgc tcatcaagta
    12721 ctggatcact tagttctaat gctctaggag atactacagt atgattcttc tcttggacag
    12781 gaaatatatc cacattgtcc aaagcttcaa ccatctgaac agaatccatt tctgaaaata
    12841 taatagattc atcattggaa caagtcaact gctggtgggt atcttgattc tcatttacat
    12901 ttgatttttt aattaggtct tcttgtagac acagagaatc actaaaatgg tttgatgtta
    12961 cttctgaagg aaggggttct ttcatttgca tatcaggtaa ttgttctttt actagatagt
    13021 catcactgaa gctatcaaat agtaaactat cactcatatt caaacttgtt tcaggaactg
    13081 gaagacattc tccttctaca ccagtgggag ttctcttttg aggaatcaga agtataactg
    13141 gtttcacagt ttcttgtgtt tgataacctt gaagaaaact atttaattgt gaatcagtaa
    13201 cagaaacttc attctttttt aaaaaaagac cattttcctc caatagtatt ggagaatgga
    13261 aaatcgggct ttctggagtc aggatatcaa ccccatgtga atcactgctt tgtttcatag
    13321 tacctacagt cttaaggtcc aaatgatcta tcttagtcgc tcctagaggg tgctgttcag
    13381 caggaaaagg aatgtgacac tccttctgaa aagagttcat tgagttctgt tggactaagc
    13441 tcttctgcat tatccctgct gccaggttgg tgtcctttgc tcctagtttg gcattttcag
    13501 ttgccatctg ttgtattatt ttctctgact gagtatccag atagaaacta tcttcaaaat
    13561 cacagaggac taaacctaag tcagaaacat gattattttt agttttgttt gttgtataag
    13621 tacctgtttt ttcttgtagt ctagaaatat ttagaaaatt ctcatgctgg ccttctgatt
    13681 tgctaaatgc tccagctgat ggaagtactt cactgggtat cacagttctg cttatatcat
    13741 ctcctaatgc ctgaaaatga cttggtttat tttcctctgt attaagcttt atcctttcac
    13801 aattgatagt aacatttgag tctctattta tgtaactact gactgcttca cagggcattt
    13861 gtctctctat tatatttttc tgtttggtaa tagtgcttgt ctgttcatga gattgctttc
    13921 gcaggtactg gttaattgga tggatgtcat ggtgtttcat ataaacattc tggttttttg
    13981 aaccattttg tatcagcact tcatttattt tttctgcctc aacagctact cctctgccct
    14041 tttcactaac cacactagtg gcctgacaag tcacattttt actctgagat cctgtgacaa
    14101 tatgctccac aaaattatca ttagttagtg ttatgttcca tgaacaattt tgctttattt
    14161 gtaatgggaa tgatgtttta ttatcttttt ccttaccact caaagataca tttttagcaa
    14221 atggccctga atttctaaat tccgttttct tctcatctaa ggtaaacggg tcttcacaaa
    14281 gactaggatt agacagagtc tgtccttgta aatggattct acacgctcca gagtctttca
    14341 gggggctgct gtccctagat cgctttagat gctttctacg tttccaagat cgaaaacttc
    14401 tgctcatctt ttctgaattg aaattcaaag gtgccttttt tgttttctgt gaaaaagtct
    14461 gaacaacttt cttatcactt gttttcccct cattctgaga ggctcctggc ttctctttat
    14521 ttatatctaa agaggcccgt tttcttgctc tgaaaatgga acatctctga tgttcttgat
    14581 tcccattctg gaaattacaa ttaaaggaac ttgtatgctc tctactttta tttaagtctt
    14641 gcactatatt tggtgaatgc ttaatataag aatcactaaa tattgtgtta cttttgttct
    14701 ttgatgttaa ttttttataa gaactcttag tttgggatat aaatgtgtgt tcctttactt
    14761 cggactcact atgagtcaat gagcatgtac tagaatgtaa cagggcacat ggattccatt
    14821 gcactcccat ttcaactaag tcctgctgca gaatcattct ggcttcttcc actataaggg
    14881 ctgctgcttc cctttcagtt aaaccttttc tgccagtcac ccagatagtt cgcatattgc
    14941 gacgttcttc aactgcttcc tcttcctcat ccactgcctt ccgggcactt ttgaaaggca
    15001 cagcattttt cagaatcacc tccacctcca caatatttgc tctagcaagg tctgccacag
    15061 tatgaaagcc agaagcatag agaaccctgg ctctctgagc atttagtaag gatacccgaa
    15121 ccaggtcaca cagctccctc tggatgccaa acgtaagacg cttctgaaat tgggaaagta
    15181 gtagttccat gttgtgccag cccagacggt tggaaaatac tgtaatcatc cctgcataaa
    15241 cagcagctga ctgttgcaaa gattgaatct gcccacgatt gcatccatat ttctgattta
    15301 tttcccttaa gggaacttca ctgattaaat ctaatagcac aagactggtg aaaaaccttt
    15361 tatggatggc catttgtcga tgctgtctct cagttctggc tactactttt cctttcacac
    15421 aacgggccaa gaacccctct tcaactccca ctagctctgc cacccttttc attgaagttg
    15481 gcaacttctc c
//
