LOCUS       sequence-451057-        8058 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MTK3 part containing the coding sequence of PEmax.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8058)
  AUTHORS   Loveless BT, Carlson CK, Dentzel Helmy CA, Hu VJ, Ross SK, Demelo 
            MC, Murtaza A, Liang G, Ficht M, Singhai A, Pajoh-Casco MJ, Liu CC
  TITLE     Open-ended molecular recording of sequential cellular events into 
            DNA
  JOURNAL   bioRxiv 2021.11.05.467507
REFERENCE   2  (bases 1 to 8058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.11.05.467507"
COMMENT     Sequence Label: pTBL2670 MTK3-PEmax
FEATURES             Location/Qualifiers
     source          1..8058
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(657..677)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     rep_origin      complement(825..1413)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(905..924)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1595..2254
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1661..1680)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2325..2359
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(2379..2405)
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             complement(2415..2435)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(4536..4556)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(4614..8058,1..656))
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 ctgctcttgc tcagtctggc agacaggatg gccttggcgt ccacgccgct ggcgttgatg
       61 gggttttcct cgaacagctg gttgtaggtc tgcaccagct ggatgaacag cttgtccacg
      121 tcgctgttgt cggggttcag gtcgccctcg atcaggaagt ggccccggaa cttgatcatg
      181 tgggccaggg ccagatagat cagccgcagg tcggccttgt cggtgctgtc caccagtttc
      241 tttctcaggt ggtagatggt ggggtacttc tcgtggtagg ccacctcgtc cacgatgttg
      301 ccgaagatgg ggtgccgctc gtgcttctta tcctcttcca ccaggaagga ctcttccagt
      361 ctgtggaaga agctgtcgtc caccttggcc atctcgttgc tgaagatctc ttgcagatag
      421 cagatccggt tcttccgtct ggtgtatctt cttctggcgg ttctcttcag ccgggtggcc
      481 tcggctgttt cgccgctgtc gaacagcagg gctccgatca ggttcttctt gatgctgtgc
      541 cggtcggtgt tgcccagcac cttgaatttc ttgctgggca ccttgtactc gtcggtgatc
      601 acggcccagc ccacagagtt ggtgccgatg tccaggccga tgctgtactt cttgtcgact
      661 ttccgcttct tctttggtga ctcgaactcg cttccgtcgg ctgtccgttt catatgagac
      721 cgactacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
      781 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
      841 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
      901 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
      961 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     1021 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     1081 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     1141 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     1201 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     1261 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     1321 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     1381 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     1441 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgat tgatcgggca
     1501 cgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg tattttttga
     1561 gttatcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac
     1621 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
     1681 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
     1741 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
     1801 tcatccggaa tttcgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
     1861 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata
     1921 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
     1981 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgttt cagccaatcc
     2041 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
     2101 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
     2161 tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca
     2221 acagtactgc gatgagtggc agggcggggc gtaatttgat atcgagctcg cttggactcc
     2281 tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga acgctcggtt
     2341 gccgccgggc gttttttatt ggtctggtct caggatccgt ccagcttcac tctcttagcg
     2401 gcagggccag agccgacttt cctcttcttc ttgggagact cgaattcgct gccgtcggcg
     2461 gttctttttg agccgccaga aggggaggag ttttcaatca gcagagtgga agtgtcggga
     2521 gtttcagtaa tagcagcctt tctggcggcc tggtcggcca tccgattgcc ccttgcctcg
     2581 gcggagtgtc ccttctggtg tccaggacag tggatgatgc tcagccgctt gggcagaaac
     2641 agggccttca gcagggccag gatctcatcc ttgttcttga tctccttgcc ctcagaggtc
     2701 agccagcccc ggcgcctgta gatctctccg tggatgtgtg cggtggcgaa ggcatatctg
     2761 gagtctgtgt acacgttcag cttcttgccc tcggccatct tcagggcctg ggtcagggcg
     2821 atcagctcgg cccgctgggc ggatgtgcct gctggcaggg ccttggccca gatcacttct
     2881 gtctctgtgg tcactgctgc tcctgccttc ctctggccct cctgcagcag ggagcttcca
     2941 tcggtatacc atgtgtgatc ggcgtcaggc aggggctgat cggtcaggtc tggccttgtt
     3001 ccgtgtgcct ctgccaggat gtccagacag ttgtgctgca ggccctcctc tggcagaggc
     3061 agcagggtgg ctggattcag ggccaccaca gggccaaact gcacgcgatc ggtgtccagc
     3121 agcagggcct ggtagtgtgt catccgggcg ttagacagcc agcgatctgg aggctgcttc
     3181 accagggcct ccactgcgtg tggtgccagg atgaccagtg gctgtcccat ggtcagcttg
     3241 ccggcatcct ttgtcagcac ggcgattgct gccaccatcc tcaggcatgg tggccatcct
     3301 gctgccactg ggtccagctt cttggacaga taggccacgg gccgcctcca tggtcccagc
     3361 ttctgggtca gcacgccctt ggcgtagccc tgcttctcat ccacaaacag ctcgaaaggc
     3421 ttggtcaggt ctggcaggcc cagggctggt gctgtcagca gggcctgctt gatctcctga
     3481 taggccttct gctggtcagg gccccaatta aacagggtgc ccggcttggt cagtgggtac
     3541 agaggtgctg ccatctctgc gaatcctggg atgaacagcc tgcaaaatcc tgctttgccc
     3601 aggaactccc tcagctgtct tggggtcttg ggtgttggct ggcccatcac tgtctccttc
     3661 ctggcctctg tcagccatct ctggccctcc ttcagcagat agcccaggta cttcacctgc
     3721 ttctgacaga tctgggcctt cttggcggat gccctatatc ccaggtttcc cagggtctgc
     3781 agcagggcgc gtgttccctg ctggcaatcc agctcgctgg tggcggccag cagcagatcg
     3841 tccacgtact gcagcaggat cagatctggg tgctggatcc ggaaatctgc caggtcgcgg
     3901 tgcagggcct cgttaaacag tgttgggcta ttcttgaagc cctgaggcag gcgtgtccag
     3961 gtcagctggc cagagatgcc catctcaggg tccctccact caaaggcgaa cagtggctgg
     4021 ctggtagggt gcagtctcag acaaaagaag gcatccttca ggtccagcac tgtataccac
     4081 tggtgagaag ggggcaggcc ggacagcaga ttgtaagggt tgggcacggt ggggtggata
     4141 tcctccaccc tcttgttcac ctctctcaga tcctgcacgg gtctatagtc attggtgcct
     4201 ggcttcttca cgggcagcag tggtgtgttc caggggctct ggcatggcac caggatgccc
     4261 tggtccagca gcctctggat gtgaggcttg atgcccagtc tggcctcctg agacataggg
     4321 tactgcttga tgctcacggg tgtagaggtg gccttcagag ggatgatcag gggtgcctgc
     4381 cgcacggcca ggcccattcc tcctgtctcg gcccaggcct ggggaaagtc ggacagccag
     4441 gtggagccca gggacacgtc gggttccttg cttgtttcat gcagtctata ctcgtcttca
     4501 atgttcaggg tgcttccgcc gctagagcct ccgctcacct ttcttttctt cttagggctc
     4561 tcgaattcag agccgtcggc ggtccgcttg ctgccaccag agcttccgcc ggagtcacct
     4621 cccagctgag acaggtcgat ccgtgtctcg tacaggccgg tgatgctctg gtggatcagg
     4681 gtggcgtcca gcacctcttt ggtgctggtg tacctcttcc ggtcgatggt ggtgtcaaag
     4741 tacttgaagg cggcaggggc tcccagattg gtcagggtaa acaggtggat gatattctcg
     4801 gcctgctctc tgatgggctt atcccggtgc ttgttgtagg cggacagcac tttgtccaga
     4861 ttagcgtcgg ccaggatcac tctcttggag aactcgctga tctgctcgat gatctcgtcc
     4921 aggtagtgct tgtgctgttc cacaaacagc tgtttctgct cattatcctc gggggagccc
     4981 ttcagcttct catagtggct ggccaggtac aggaagttca catatttgga gggcagggcc
     5041 agttcgtttc ccttctgcag ttcgccggca gaggccagca ttctcttccg gccgttttcc
     5101 agctcgaaca gggagtactt aggcagcttg atgatcaggt cctttttcac ttctttgtag
     5161 cccttggctt ccagaaagtc gatgggattc ttctcgaagc tgcttctttc catgatggtg
     5221 atccccagca gctctttcac actcttcagt ttcttggact tgcccttttc cactttggcc
     5281 accaccagca cagaataggc cacggtgggg ctgtcgaagc cgccgtactt cttagggtcc
     5341 cagtccttct ttctggcgat cagcttatcg ctgttcctct tgggcaggat agactctttg
     5401 ctgaagccgc ctgtctgcac ctcggtcttt ttcacgatat tcacttgggg catgctcagc
     5461 actttccgca cggtggcaaa atcccggccc ttatcccaca cgatctcccc ggtttcgccg
     5521 tttgtctcga tcagaggccg cttccggatc tcgccgttgg ccagggtaat ctcggtcttg
     5581 aaaaagttca tgatgttgct gtagaagaag tacttggcgg tagccttgcc gatttcctgc
     5641 tcgctcttgg cgatcatctt ccgcacgtcg tacaccttgt agtcgccgta cacgaactcg
     5701 ctttccagct tagggtactt tttgatcagg gcggttccca cgacggcgtt caggtaggcg
     5761 tcgtgggcgt ggtggtagtt gttgatctcg cgcactttgt aaaactggaa atccttccgg
     5821 aaatcggaca ccagcttgga cttcagggtg atcactttca cttcccggat cagcttgtca
     5881 ttctcgtcgt acttagtgtt catccgggag tccaggatct gtgccacgtg ctttgtgatc
     5941 tgccgggttt ccaccagctg tctcttgatg aagccggcct tatccagttc gctcaggccg
     6001 cctctctcgg ccttggtcag attgtcgaac tttctctggg taatcagctt ggcgttcagc
     6061 agctgccgcc agtagttctt catcttcttc acgacctctt cggagggcac gttgtcgctc
     6121 ttgccccggt tcttgtcgct tctggtcagc accttgttgt cgatggagtc gtccttcaga
     6181 aagctctgag gcacgatagc gtccacatcg tagtcggaca gccggttgat gtccagttcc
     6241 tggtccacgt acatatcccg cccattctgc aggtagtaca ggtacagctt ctcgttctgc
     6301 agctgggtgt tttccacggg gtgttctttc aggatctggc tgcccagctc tttgatgccc
     6361 tcttcgatcc gcttcattct ctcgcggctg ttcttctgtc ccttctgggt ggtctggttc
     6421 tctctggcca tttcgatcac gatgttctcg ggcttgtgcc ggcccatcac tttcacgagc
     6481 tcgtccacca ccttcactgt ctgcaggatg cccttcttaa tggcggggct gccggccaga
     6541 ttggcaatgt gctcgtgcag gctatcgccc tggccggaca cctgggcttt ctggatgtcc
     6601 tctttaaagg tcaggctgtc gtcgtggatc agctgcatga agtttctgtt ggcgaagccg
     6661 tcggacttca ggaaatccag gattgtcttg ccggactgct tgtcccggat gccgttgatc
     6721 agcttccggc tcagcctgcc ccagccggtg tatctccgcc gcttcagctg cttcatcact
     6781 ttgtcgtcga acaggtgggc ataggttttc agccgttcct cgatcatctc tctgtcctca
     6841 aacagtgtca gggtcagcac gatatcttcc agaatgtcct cgttttcctc attgtccagg
     6901 aagtccttgt ccttgataat tttcagcaga tcgtggtatg tgcccaggga ggcgttgaac
     6961 cgatcttcca cgccggagat ttccacggag tcgaagcact cgattttctt gaagtagtcc
     7021 tctttcagct gcttcacggt cactttccgg ttggtcttga acagcaggtc cacgatggcc
     7081 tttttctgct cgccgctcag gaaggcgggc tttctcattc cctcggtcac gtatttcact
     7141 ttggtcagct cgttatacac ggtgaagtac tcgtacagca ggctgtgctt gggcagcacc
     7201 ttctcgttgg gcaggttctt atcgaagttg gtcatccgct cgatgaagct ctgggcggaa
     7261 gcgcccttgt ccaccacttc ctcgaagttc cagggggtga tggtttcctc gctctttctg
     7321 gtcatccagg cgaatctgct gtttcccctg gccagagggc ccacgtagta ggggatgcgg
     7381 aaggtcagga tcttctcgat cttttcccgg ttgtccttca ggaatgggta aaaatcttcc
     7441 tgccgccgca gaatggcgtg cagctctccc aggtggatct ggtgggggat gctgccgttg
     7501 tcgaaggtcc gctgcttccg cagcaggtcc tctctcttca gcttcacgag cagttcctcg
     7561 gtgccgtcca tcttttccag gatgggcttg atgaacttgt agaactcttc ctggctggct
     7621 ccgccgtcaa tgtagccggc gtagccgttc ttgctctggt cgaagaaaat ctctttgtac
     7681 ttctcaggca gctgctgccg cacgagagct ttcagcaggg tcaggtcctg gtggtgctcg
     7741 tcgtatctct tgatcataga ggcgctcagg ggggccttgg tgatctcggt gttcactctc
     7801 aggatgtcgc tcagcaggat ggcgtcggac aggttcttgg cggccagaaa caggtcggcg
     7861 tactggtcgc cgatctgggc cagcaggttg tccaggtcgt cgtcgtaggt gtccttgctc
     7921 agctgcagtt tggcatcctc ggccaggtcg aagttgctct tgaagttggg ggtcaggccc
     7981 aggctcaggg caatcaggtt tccgaacagg ccattcttct tctcgccggg cagctgggcg
     8041 atcagatttt ccagcttt
//
