LOCUS       sequence-451059-        2787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MTK3 part containing the coding sequence of LacI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Loveless BT, Carlson CK, Dentzel Helmy CA, Hu VJ, Ross SK, Demelo 
            MC, Murtaza A, Liang G, Ficht M, Singhai A, Pajoh-Casco MJ, Liu CC
  TITLE     Open-ended molecular recording of sequential cellular events into 
            DNA
  JOURNAL   bioRxiv 2021.11.05.467507
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.11.05.467507"
COMMENT     Sequence Label: pTBL2621 MTK3-LacI
FEATURES             Location/Qualifiers
     source          1..2787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             617..1276
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(683..702)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1347..1381
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1407..1427)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     rep_origin      complement(join(2634..2787,1..435))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2714..2733)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
       61 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
      121 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
      181 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
      241 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
      301 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
      361 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
      421 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
      481 gaaaactcac gttaagggat tttggtcatg attgatcggg cacgtaagag gttccaactt
      541 tcaccataat gaaataagat cactaccggg cgtatttttt gagttatcga gattttcagg
      601 agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca
      661 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca
      721 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt
      781 ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg aatttcgtat
      841 ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt
      901 ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca
      961 gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc
     1021 taaagggttt attgagaata tgtttttcgt ttcagccaat ccctgggtga gtttcaccag
     1081 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa
     1141 atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt
     1201 ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg
     1261 gcagggcggg gcgtaatttg atatcgagct cgcttggact cctgttgata gatccagtaa
     1321 tgacctcaga actccatctg gatttgttca gaacgctcgg ttgccgccgg gcgtttttta
     1381 ttggtctggt ctcaggatcc agagccaacc ttcctcttct tcttaggagg cctcaggctg
     1441 ctctgcccac tttccagtct ggaaacctgt cttgctagct gcatgagtga atcagccaat
     1501 gcccggggag aggcagtttg tgtattgggt gccagggtgg tttttctctt caccagtgag
     1561 actggcaaca gctgattgcc cttcaccgcc tggccctgag agagttgcag caagcggtcc
     1621 acgctggttt gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa
     1681 catgagctgt cttcggtatc gtcgtatccc actaccgaga tatctgcacc aactctcagc
     1741 ccagactcag taatggctct cattgcaccc agtgccatct gatcattggc aaccagcatt
     1801 gcagtgggaa caatgccctc attcagcatt tgcatggttt gttgaaaccc agacatggca
     1861 ctccagtccc cttctctttc agctattggc tgaatttgat tcctagtgag atatttatgc
     1921 cagccagcca gtctcagcct tgctgagaca gaactgagtg ggcccgcaag cagtgcaatt
     1981 tgctggtgtc ccaatgcaac cagatgctcc acacccagtc ttgtaccatc ttcatgggag
     2041 aaaataatac tgttgatggg tgtctggtca gagacatcaa gaaagagtgc tggaacatta
     2101 gtgcaggcag cttccacagc aatggcatcc tggtcatcca gtggatagtt aatgatcagc
     2161 ccactgactc tttgtgccag aagattgtgc acagcagctt tacaggcttc aactccactt
     2221 ctttctacca ttgacaccac cacagaggct cccagttgat cagctctaga tttaatggct
     2281 gccacaattt gagatggtgc atgcagggcc agactggagg tggcaactcc aatcagcaag
     2341 ctctgtttgc ctgccagttg ttgtgccact ctgttgggaa tgtaattcag ctctgccatg
     2401 gctgcttcca ctttttccct ggttttggca gaaacatggc tggcctggtt caccactctg
     2461 gaaacagtct gataagagac accggcatac tctgcgacat catacaatgt tactggtttc
     2521 atatgagacc gactacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     2581 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     2641 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     2701 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     2761 gttccgaccc tgccgcttac cggatac
//
