LOCUS       sequence-45106-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Horst D, Chen J, Morikawa T, Ogino S, Kirchner T, Shivdasani RA
  TITLE     Differential WNT activity in colorectal cancer confers limited 
            tumorigenic potential and is regulated by MAPK signaling.
  JOURNAL   Cancer Res. 2012 Feb 8.
  PUBMED    22318865
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2012 Feb 8."
COMMENT     Sequence Label: TOP-GFP.mC
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(121..498)
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAVTKYTSAK"
     primer_bind     complement(576..594)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 cctgcctgcc tcgatctcga actcgtggcc gttcacggag ccctccatgt gcaccttgaa
       61 gcgcatgaac tccttgatga tggccatgtt atcctcctcg cccttgctca ccatcggatc
      121 cttagcgctg gtgtacttgg tgacggcctt agtaccctcg gacacggcgt gcttggccaa
      181 ctccccaggc agcagcaggc gcacggccgt ctggatctcc ctggaggtga tggtcgagcg
      241 cttgttgtaa tgcgccaggc gggaagcctc acctgcgatg cgctcgaaaa tgtcgttcac
      301 aaacgaattc atgatgccca tggccttgga cgaaatgccg gtgtcagggt ggacctgctt
      361 cagaaccttg tacacataga tggaatagct ctccttgcgg ctgcgcttgc gcttcttgcc
      421 gtctttcttc tgcgccttag tcaccgcctt cttggagccc tttttcgggg cgggagcaga
      481 cttcgctggc tctggcatgg tggcggatcc ccctggggag agaggtcggt gattcggtca
      541 acgagggagc cgactgccga cgtgcgctcc ggaggcttgc agaatgcgga acaccgcgcg
      601 ggcaggaaca gggcccacac taccgcccca caccccgcct cccgcaccgc cccttcccgg
      661 ccgctgctct cggcgcgccc tgctgagcag ccgctattgg ccacagccca tcgcggtcgg
      721 cgcgctgcca ttgctccctg gcgctgtccg tctgcgaggg tactagtgag acgtgcggct
      781 tccgtttgtc acgtccggca cgccgcgaac cgcaaggaac cttcccgact
//
