LOCUS       sequence-451080-        2391 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MTK3 part containing the coding sequence of mTagBFP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Loveless BT, Carlson CK, Dentzel Helmy CA, Hu VJ, Ross SK, Demelo 
            MC, Murtaza A, Liang G, Ficht M, Singhai A, Pajoh-Casco MJ, Liu CC
  TITLE     Open-ended molecular recording of sequential cellular events into 
            DNA
  JOURNAL   bioRxiv 2021.11.05.467507
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.11.05.467507"
COMMENT     Sequence Label: pTBL3445 MTK3-mTagBFP2
FEATURES             Location/Qualifiers
     source          1..2391
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(16..604)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(96..115)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             786..1445
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(852..871)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1516..1550
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1567..1587)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(1597..2295)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
ORIGIN
        1 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
       61 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      121 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      181 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      241 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      301 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
      361 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
      421 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
      481 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
      541 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
      601 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
      661 ttaagggatt ttggtcatga ttgatcgggc acgtaagagg ttccaacttt caccataatg
      721 aaataagatc actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag
      781 ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta
      841 aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc
      901 tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct
      961 ttattcacat tcttgcccgc ctgatgaatg ctcatccgga atttcgtatg gcaatgaaag
     1021 acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa
     1081 ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca
     1141 tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta
     1201 ttgagaatat gtttttcgtt tcagccaatc cctgggtgag tttcaccagt tttgatttaa
     1261 acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc
     1321 aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct
     1381 tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg
     1441 cgtaatttga tatcgagctc gcttggactc ctgttgatag atccagtaat gacctcagaa
     1501 ctccatctgg atttgttcag aacgctcggt tgccgccggg cgttttttat tggtctggtc
     1561 tcaggatacc ttccttttct tttttggacc tccaccatta agcttgtgcc ccagtttgct
     1621 agggaggtcg cagtatctgg ccactgccac ctcgtgctgc tcgacgtagg tttcgttgtt
     1681 ggcctccttg attctttcca gtctgtagtc cacatagtag acgccaggca tcttgaggtt
     1741 cttagcgggt ttcttggatc tatatgtggt cttgatgttt gcgatcagat ggctcccgcc
     1801 cacgagcttc agggccatgt cgtttctgcc ttccaggccg ccgtcagcgg ggtacagcgt
     1861 ttcggtgaag gcctcccagc cgagtgtttt cttctgcatc acagggccgt tggatgtgaa
     1921 gttcacccct ctgatcttga cgttgtagat gaggcagccg tcctggaggc tggtgtcctg
     1981 ggtagcggtc agcacgcccc cgtcttcgta tgtggtgact ctctcccatg tgaagccctc
     2041 agggaaggac tgcttgaaga agtcggggat gccctgggtg tggttgatga aggtcttgct
     2101 gccgtagagg aagctagtag ccaggatgtc gaaggcgaag gggagagggc cgccctcgac
     2161 caccttgatt ctcatggtct gggtgccctc gtagggcttg ccttcgccct cggatgtgca
     2221 cttgaagtga tggttgtcca cggtgccctc catgtacagc ttcatgtgca tgttctcctt
     2281 aatcagctcg ctcatatgag accgactacg gttatccaca gaatcagggg ataacgcagg
     2341 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg c
//
