LOCUS       sequence-451102-        2385 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MTK3 part containing the coding sequence of mCherry..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Loveless BT, Carlson CK, Dentzel Helmy CA, Hu VJ, Ross SK, Demelo 
            MC, Murtaza A, Liang G, Ficht M, Singhai A, Pajoh-Casco MJ, Liu CC
  TITLE     Open-ended molecular recording of sequential cellular events into 
            DNA
  JOURNAL   bioRxiv 2021.11.05.467507
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.11.05.467507"
COMMENT     Sequence Label: pTBL3481 MTK3-mCherry
FEATURES             Location/Qualifiers
     source          1..2385
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             309..968
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(375..394)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1039..1073
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1105..1812)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     1649..1667
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     rep_origin      complement(join(1924..2385,1..127))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2004..2023)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
       61 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
      121 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
      181 acgttaaggg attttggtca tgattgatcg ggcacgtaag aggttccaac tttcaccata
      241 atgaaataag atcactaccg ggcgtatttt ttgagttatc gagattttca ggagctaagg
      301 aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc
      361 gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc
      421 agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg
      481 cctttattca cattcttgcc cgcctgatga atgctcatcc ggaatttcgt atggcaatga
      541 aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc
      601 aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac
      661 acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt
      721 ttattgagaa tatgtttttc gtttcagcca atccctgggt gagtttcacc agttttgatt
      781 taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata
      841 cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg
      901 gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcagggcg
      961 gggcgtaatt tgatatcgag ctcgcttgga ctcctgttga tagatccagt aatgacctca
     1021 gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtctg
     1081 gtctcaggat tcgacatctg tagacttgta cagctcgtcc atgccgccgg tggagtggcg
     1141 gccctcggcg cgttcgtact gttccacgat ggtgtagtcc tcgttgtggg aggtgatgtc
     1201 caacttgatg ttgacgttgt aggcgccggg cagctgcacg ggcttcttgg ccttgtaggt
     1261 ggtcttgacc tcagcgtcgt agtggccgcc gtccttcagc ttcagcctct gcttgatctc
     1321 gcccttcagg gcgccgtcct cggggtacat ccgctcggag gaggcctccc agcccatggt
     1381 tttcttctgc attacggggc cgtcggaggg gaagttggtg ccgcgcagct tcaccttgta
     1441 gatgaactcg ccgtcctgca gggaggagtc ctgggtcacg gtcaccacgc cgccgtcctc
     1501 gaagttcatc acgcgctccc acttgaagcc ctcggggaag gacagcttca agtagtcggg
     1561 aatgtcggcg gggtgcttca cgtaggcctt ggagccgtac atgaactgag gggacaggat
     1621 gtcccaggcg aagggcaggg ggccaccctt ggtcaccttc agcttggcgg tctgggtgcc
     1681 ctcgtagggg cggccctcgc cctcgccctc gatctcgaac tcgtggccgt tcacggagcc
     1741 ctccatgtgc accttgaagc gcatgaactc cttgatgatg gccatgttat cctcctcgcc
     1801 cttgctcacc atatgagacc gactacggtt atccacagaa tcaggggata acgcaggaaa
     1861 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     1921 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     1981 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     2041 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     2101 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     2161 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     2221 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     2281 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     2341 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgc
//
