LOCUS       sequence-451103-        2298 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MTK3 part containing the coding sequence of TetR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Loveless BT, Carlson CK, Dentzel Helmy CA, Hu VJ, Ross SK, Demelo 
            MC, Murtaza A, Liang G, Ficht M, Singhai A, Pajoh-Casco MJ, Liu CC
  TITLE     Open-ended molecular recording of sequential cellular events into 
            DNA
  JOURNAL   bioRxiv 2021.11.05.467507
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.11.05.467507"
COMMENT     Sequence Label: pTBL2620 MTK3-TetR
FEATURES             Location/Qualifiers
     source          1..2298
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             372..1031
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(438..457)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1102..1136
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     rep_origin      complement(join(1900..2298,1..190))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1980..1999)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
       61 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
      121 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
      181 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
      241 ctcacgttaa gggattttgg tcatgattga tcgggcacgt aagaggttcc aactttcacc
      301 ataatgaaat aagatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta
      361 aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc
      421 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg
      481 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc
      541 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattt cgtatggcaa
      601 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg
      661 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc
      721 tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag
      781 ggtttattga gaatatgttt ttcgtttcag ccaatccctg ggtgagtttc accagttttg
      841 atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt
      901 atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg
      961 atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg
     1021 gcggggcgta atttgatatc gagctcgctt ggactcctgt tgatagatcc agtaatgacc
     1081 tcagaactcc atctggattt gttcagaacg ctcggttgcc gccgggcgtt ttttattggt
     1141 ctggtctcag gatccgcttc ccgtacgact tccactctca cacttcagct gcttctccag
     1201 gccgcagatg ataagctcca agccgaacag gaaggccggc tcggcaccct ggtggtcgaa
     1261 caactcaatt gcctgccgca agagaggtgg catagagtcg gtggtagggg tttcccgttc
     1321 ctccttagcc acttgatgct cctggtcctc cagcacgcag cccagggtaa aatgacccac
     1381 ggctgacagc gcgtaaagcg cgttttcgag agagaagccc tgctgacaca aaaaggccag
     1441 ttgattttcc agtgtctcat actgcttttc cgtgggtctt gtacccagat gcaccttagc
     1501 accgtcccga tgagacagaa gagcgcatct aaatgacttg gcattgtttc gcaggaagtc
     1561 ctgccaggat tccccttcaa gggggcaaaa gtgtgtatgg tgtctgtcga gcatctcaat
     1621 tgccagcgcg tccaggagag cccttttatt cttgacgtgc cagtacaggg ttggctgttc
     1681 gacgcccagc ttttgggcca gcttcctcgt tgtgaggccc tctatgccta cctcgttcag
     1741 caattccagt gcagagttga ttactttaga tttgtccaac cgagacatat gagaccgact
     1801 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     1861 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     1921 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     1981 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     2041 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     2101 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     2161 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     2221 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     2281 gtatgtaggc ggtgctac
//
