LOCUS       sequence-451104-        2376 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MTK3 part containing the coding sequence of sfGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Loveless BT, Carlson CK, Dentzel Helmy CA, Hu VJ, Ross SK, Demelo 
            MC, Murtaza A, Liang G, Ficht M, Singhai A, Pajoh-Casco MJ, Liu CC
  TITLE     Open-ended molecular recording of sequential cellular events into 
            DNA
  JOURNAL   bioRxiv 2021.11.05.467507
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.11.05.467507"
COMMENT     Sequence Label: pTBL2142 MTK3-sfGFP
FEATURES             Location/Qualifiers
     source          1..2376
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(8..596)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(88..107)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             778..1437
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(844..863)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1508..1542
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1559..2272)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
       61 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
      121 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
      181 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
      241 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
      301 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
      361 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
      421 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
      481 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
      541 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
      601 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
      661 ttttggtcat gattgatcgg gcacgtaaga ggttccaact ttcaccataa tgaaataaga
      721 tcactaccgg gcgtattttt tgagttatcg agattttcag gagctaagga agctaaaatg
      781 gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat
      841 tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt
      901 acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac
      961 attcttgccc gcctgatgaa tgctcatccg gaatttcgta tggcaatgaa agacggtgag
     1021 ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg
     1081 ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca catatattcg
     1141 caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat
     1201 atgtttttcg tttcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc
     1261 aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac
     1321 aaggtgctga tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc
     1381 ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt
     1441 gatatcgagc tcgcttggac tcctgttgat agatccagta atgacctcag aactccatct
     1501 ggatttgttc agaacgctcg gttgccgccg ggcgtttttt attggtctgg tctcaggatt
     1561 tgtacagttc atccatacca tgcgtgatgc ccgctgcggt tacgaactcc agcagaacca
     1621 tatgatcgcg tttctcgttc ggatctttag acagaacgct ttgcgtgctc agatagtgat
     1681 tgtctggcag cagaacagga ccatcaccga ttggagtgtt ttgctggtag tgatcagcca
     1741 gctgcacgct gccatcctcc acgttgtggc gaattttaaa attcgcttta atgccatttt
     1801 tttgtttatc ggcggtgatg taaacattgt ggctgttaaa attgtattcc agcttatggc
     1861 ccaggatatt gccgtcctct ttaaagtcaa tgcctttcag ctcaatgcgg tttaccaggg
     1921 tatcgccttc aaatttcact tccgcacgcg ttttgtacgt gccgtcatcc ttaaaggaaa
     1981 tcgtgcgttc ctgcacatag ccttccggca tggcggactt gaagaagtca tgctgcttca
     2041 tatggtccgg ataacgagca aagcactgaa caccataagt cagcgtcgtt accagagtcg
     2101 gccaaggaac cggcagttta ccagtagtac agatgaactt cagcgtcagt ttaccattag
     2161 ttgcgtcacc ttcaccctcg ccacgcacgg aaaacttatg accgttgaca tcaccatcca
     2221 gttccaccag aatagggacg acaccagtga acagctcttc gcctttacgc atatgagacc
     2281 gactacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     2341 agcaaaaggc caggaaccgt aaaaaggccg cgttgc
//
