LOCUS       sequence-451129-        4583 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For gateway cloning of PARP15.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Weixler L, Ikenga NJ, Voorneveld J, Aydin G, Bolte TM, Momoh J, 
            Butepage M, Golzmann A, Luscher B, Filippov DV, Zaja R, Feijs KL
  TITLE     Protein and RNA ADP-ribosylation detection is influenced by sample 
            preparation and reagents used.
  JOURNAL   Life Sci Alliance. 2022 Nov 11;6(1):e202201455. doi: 
            10.26508/lsa.202201455.
  PUBMED    36368907
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2022-11-11"; volume: "6(1):e202201455. doi"; 
            pages: " 10.26508/lsa.202201455"
COMMENT     Sequence Label: pDONR221-PARP15
FEATURES             Location/Qualifiers
     source          1..4583
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     94..111
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(270..297)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(389..475)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     524..546
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     538..555
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     539..555
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    571..670
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2710..2809)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2826..2845)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2827..2845)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2850..2866)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2850..2866)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2968..2987)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2979..3788
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3046..3065)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3935..4523
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4424..4443
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
       61 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      121 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      181 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc
      241 gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct
      301 tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct
      361 tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca
      421 aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc
      481 tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc acgacgttgt
      541 aaaacgacgg ccagtcttaa gctcgggccc caaataatga ttttattttg actgatagtg
      601 acctgttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaac tttgtacaaa
      661 aaagcaggct tcatggctgc gccaggcccc cttcctgccg ctgctctgag tccaggggct
      721 ccgaccccca gagaacttat gcacggagtt gcaggtgtta cttccagagc cggacgagat
      781 cgggaggcgg ggagcgtgct gccggccggg aaccgtgggg cgcggaaggc ctcccggcgc
      841 tcttcctccc ggagtatgtc cagagacaac aagttcagca agaaagattg tctttcaatc
      901 aggaatgttg tagcttcaat ccaaaccaaa gaaggtctga atctcaagtt gataagtgga
      961 gatgttctgt acatctgggc cgatgtcatt gtcaacagcg ttcccatgaa tcttcagctt
     1021 ggaggaggac cactatctcg ggcatttttg cagaaagctg gtcccatgct ccagaaagag
     1081 ttagatgaca gaaggcggga aacagaggaa aaagtaggta acatattcat gacaagcggc
     1141 tgcaatctgg actgcaaagc tgtgctccat gctgtggctc catactggaa taatggagca
     1201 gagacttctt ggcagatcat ggcaaatata atcaagaaat gtttgacaac tgtagaagtg
     1261 ctatctttct catcaatcac atttcccatg attggaacag gaagtttgca gtttcccaaa
     1321 gctgtttttg ctaaactaat cctttcagaa gtgttcgaat acagtagcag cacaaggccg
     1381 ataactagcc ctttacaaga agtccacttt ctggtatata caaatgacga tgaaggctgt
     1441 caggcatttt tagatgaatt cactaactgg tcaagaataa atcccaacaa ggccaggatt
     1501 cccatggcag gagataccca aggtgtggtc gggactgtct ctaagccttg tttcacagca
     1561 tatgaaatga aaatcggtgc aattactttt caggttgcta ctggagatat agccactgaa
     1621 caggtagatg ttattgtaaa ctcaacagca aggacattta atcggaaatc aggtgtgtca
     1681 agagctattt tagaaggtgc tggacaagct gtggaaagtg aatgtgctgt actagctgca
     1741 cagcctcaca gagattttat aattacacca ggtggatgct taaagtgcaa aataataatt
     1801 catgttcctg ggggaaaaga tgtcaggaaa acggtcacca gtgttctaga agagtgtgaa
     1861 cagaggaagt acacatcggt ttcccttcca gccattggaa caggaaatgc cggaaaaaac
     1921 cctatcacag ttgctgataa cataatcgat gctattgtag acttctcatc acaacattcc
     1981 accccatcat taaaaacagt taaagttgtc atttttcaac ctgagctgct aaatatattc
     2041 tacgacagca tgaaaaaaag agacctctct gcatcactga actttcagtc cacattctcc
     2101 atgactacat gtaatcttcc tgaacactgg actgacatga atcatcagct gttttgcatg
     2161 gtccagctag agccaggaca atcagaatat aataccataa aggacaagtt cacccgaact
     2221 tgttcttcct acgcaataga gaagattgag aggatacaga atgcatttct ctggcagagc
     2281 taccaggtaa agaaaaggca aatggatatc aagaatgacc ataagaataa tgagagactc
     2341 ctcttccatg ggacagatgc agactcagtg ccatatgtca atcagcacgg ctttaataga
     2401 agttgtgctg ggaaaaatgc tgtatcctat ggaaaaggaa cctattttgc tgtggatgcc
     2461 agttattctg ccaaggacac ctactccaag ccagacagca atgggagaaa gcacatgtac
     2521 gttgtgcgag tacttactgg agtcttcaca aagggacgtg caggattagt cacccctcca
     2581 cccaagaatc ctcacaatcc cacagatctc tttgactcag tgacaaacaa tacacgatct
     2641 ccaaagctat ttgtggtatt ctttgataat caggcttacc cagaatatct cataactttc
     2701 acggcttaaa cccagctttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa
     2761 tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg
     2821 atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg
     2881 tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa
     2941 aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa
     3001 cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc
     3061 gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc
     3121 cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg
     3181 tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta
     3241 ctcctgatga tgcatggtta ctcaccactg cgatccccgg aaaaacagca ttccaggtat
     3301 tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc
     3361 ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg
     3421 ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc
     3481 gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac
     3541 cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga
     3601 aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg
     3661 ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa
     3721 aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt
     3781 ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg ctgacttgac
     3841 gggacggcgc aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag
     3901 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     3961 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     4021 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     4081 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     4141 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     4201 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     4261 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     4321 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     4381 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     4441 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     4501 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     4561 tttgctggcc ttttgctcac atg
//
