LOCUS       sequence-451131-        4521 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For gateway cloning of PARP7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Weixler L, Ikenga NJ, Voorneveld J, Aydin G, Bolte TM, Momoh J, 
            Butepage M, Golzmann A, Luscher B, Filippov DV, Zaja R, Feijs KL
  TITLE     Protein and RNA ADP-ribosylation detection is influenced by sample 
            preparation and reagents used.
  JOURNAL   Life Sci Alliance. 2022 Nov 11;6(1):e202201455. doi: 
            10.26508/lsa.202201455.
  PUBMED    36368907
REFERENCE   2  (bases 1 to 4521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2022-11-11"; volume: "6(1):e202201455. doi"; 
            pages: " 10.26508/lsa.202201455"
COMMENT     Sequence Label: pDONR221-PARP7
FEATURES             Location/Qualifiers
     source          1..4521
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     94..111
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(270..297)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(389..475)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     524..546
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     538..555
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     539..555
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    571..670
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2648..2747)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2764..2783)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2765..2783)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2788..2804)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2788..2804)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2906..2925)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2917..3726
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2984..3003)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3873..4461
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4362..4381
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
       61 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      121 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      181 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc
      241 gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct
      301 tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct
      361 tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca
      421 aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc
      481 tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc acgacgttgt
      541 aaaacgacgg ccagtcttaa gctcgggccc caaataatga ttttattttg actgatagtg
      601 acctgttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaac tttgtacaaa
      661 aaagcaggct tcatggaaat ggaaaccacc gaacctgagc cagactgtgt agtgcagcct
      721 ccctctcctc ctgatgactt ttcatgccaa atgagactct ctgagaagat cactccattg
      781 aagacttgtt ttaagaaaaa ggatcagaaa agattgggaa ctggaaccct gaggtctttg
      841 aggccaatat taaacactct tctagaatct ggctcacttg atggggtttt tagatctagg
      901 aaccagagta cagatgagaa cagcttacat gaacctatga tgaagaaagc catggaaatc
      961 aattcatcat gcccaccagc agaaaataat atgtctgttc tgattcctga taggacaaat
     1021 gttggggacc agataccgga agcccatcct tccactgaag ctccagaacg agtggttcca
     1081 atccaagatc acagctttcc atcagaaacc ctcagtggga cggtggcaga ttccacacca
     1141 gctcacttcc agactgatct tttgcaccca gtttcaagtg atgttcctac tagtcctgac
     1201 tgcttagaca aagtcataga ttatgttcca ggcattttcc aagaaaacag ttttacaatc
     1261 caatacattc tggacaccag tgataagctg agtactgagc tctttcagga caaaagtgaa
     1321 gaggcttccc ttgacctcgt gtttgagctg gtgaaccagt tgcagtacca cactcaccaa
     1381 gagaacggaa ttgaaatttg catggacttt ctgcaaggca cttgtattta tggcagggat
     1441 tgtttgaagc accacactgt cttgccatat cattggcaga tcaaaaggac aactactcaa
     1501 aagtggcaga gtgtattcaa tgattctcag gagcacttgg aaagatttta ctgtaaccca
     1561 gaaaatgata gaatgagaat gaagtatgga ggacaagaat tttgggcaga tttgaatgcc
     1621 atgaacgtgt atgaaacaac tgaatttgac caactacgaa ggctgtccac accaccctct
     1681 agcaatgtca actctattta ccacacagtc tggaaattct tctgtaggga ccactttgga
     1741 tggagagagt atcccgagtc tgtcattcga ttgattgaag aagccaactc tcggggtctg
     1801 aaagaggttc gatttatgat gtggaataac cactacatcc tccacaattc attcttcagg
     1861 agagagataa aaaggagacc cctcttccgc tcctgtttta tactgcttcc atatttacag
     1921 acacttggtg gggttcccac acaagctcct ccacctcttg aagcaacttc atcatcacaa
     1981 attatctgcc cagatggggt cacttcagca aacttttacc ctgaaacttg ggtttatatg
     2041 catccatctc aggacttcat ccaagtccct gtttctgcag aggataaaag ttatcggatc
     2101 atttacaatc tttttcataa gactgtgcct gagtttaaat acagaatttt gcagatattg
     2161 agagtccaaa accagtttct ttgggagaaa tataaaagga aaaaggaata tatgaacagg
     2221 aaaatgtttg gccgtgacag gataataaat gagagacatt tatttcatgg aacatcccag
     2281 gatgtggtag atggaatctg caaacacaac tttgaccctc gagtctgtgg aaagcatgct
     2341 acaatgtttg gacaaggcag ttattttgca aagaaggcaa gctactctca taacttttct
     2401 aagaagtcct ccaaaggagt ccacttcatg tttctggcca aagtgctgac gggcagatac
     2461 acaatgggca gtcatggcat gagaaggccc ccgccagtca atcctggcag tgtcaccagt
     2521 gacctttatg actcttgtgt ggataatttc tttgagcctc agatttttgt catttttaat
     2581 gatgaccaga gttaccctta ttttgttatc caatatgaag aagtcagtaa cactgtttcc
     2641 atttgatacc cagctttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt
     2701 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat
     2761 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg
     2821 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa
     2881 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg
     2941 tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
     3001 gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca
     3061 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
     3121 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
     3181 cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta
     3241 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg
     3301 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct
     3361 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt
     3421 aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg
     3481 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa
     3541 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc
     3601 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa
     3661 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt
     3721 ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg
     3781 gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg
     3841 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3901 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3961 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     4021 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     4081 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     4141 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     4201 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     4261 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     4321 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     4381 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     4441 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     4501 tgctggcctt ttgctcacat g
//
