LOCUS       sequence-451133-        4107 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For gateway cloning of LAP3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Weixler L, Zaja R, Ikenga NJ, Mohan G, Aydin G, Wijngaarden S, 
            Filippov DV, Luscher B, Feijs-Zaja KLH
  TITLE     Family-wide analysis of human macrodomains reveals novel activities
  JOURNAL   ResearchSquare. 2024 Aug 8. doi: 10.21203/rs.3.rs-4644671/v1
REFERENCE   2  (bases 1 to 4107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "ResearchSquare. 2024 Aug 8. doi: 10.21203/rs.3.rs-4644671/v1"
COMMENT     Sequence Label: pDONR221-LAP3
FEATURES             Location/Qualifiers
     source          1..4107
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2232..2331)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2348..2367)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2349..2367)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2372..2388)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2372..2388)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2490..2509)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2501..3310
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2568..2587)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3457..4045
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3946..3965
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc atgttcttgc tgcctcttcc ggctgcgggg cgagtagtcg tccgacgtct
      721 ggccgtgaga cgtttcggga gccggagtct ctccaccgca gacatgacga agggccttgt
      781 tttaggaatc tattccaaag aaaaagaaga tgatgtgcca cagttcacaa gtgcaggaga
      841 gaattttgat aaattgttag ctggaaagct gagagagact ttgaacatat ctggaccacc
      901 tctgaaggca gggaagactc gaacctttta tggtctgcat caggacttcc ccagcgtggt
      961 gctagttggc ctcggcaaaa aggcagctgg aatcgacgaa caggaaaact ggcatgaagg
     1021 caaagaaaac atcagagctg ctgttgcagc ggggtgcagg cagattcaag acctggagct
     1081 ctcgtctgtg gaggtggatc cctgtggaga cgctcaggct gctgcggagg gagcggtgct
     1141 tggtctctat gaatacgatg acctaaagca aaaaaagaag atggctgtgt cggcaaagct
     1201 ctatggaagt ggggatcagg aggcctggca gaaaggagtc ctgtttgctt ctgggcagaa
     1261 cttggcacgc caattgatgg agacgccagc caatgagatg acgccaacca gatttgctga
     1321 aattattgag aagaatctca aaagtgctag tagtaaaacc gaggtccata tcagacccaa
     1381 gtcttggatt gaggaacagg caatgggatc attcctcagt gtggccaaag gatctgacga
     1441 gcccccagtc ttcttggaaa ttcactacaa aggcagcccc aatgcaaacg aaccacccct
     1501 ggtgtttgtt gggaaaggaa ttacctttga cagtggtggt atctccatca aggcttctgc
     1561 aaatatggac ctcatgaggg ctgacatggg aggagctgca actatatgct cagccatcgt
     1621 gtctgctgca aagcttaatt tgcccattaa tattataggt ctggcccctc tttgtgaaaa
     1681 tatgcccagc ggcaaggcca acaagccggg ggatgttgtt agagccaaaa acgggaagac
     1741 catccaggtt gataacactg atgctgaggg gaggctcata ctggctgatg cgctctgtta
     1801 cgcacacacg tttaacccga aggtcatcct caatgccgcc accttaacag gtgccatgga
     1861 tgtagctttg ggatcaggtg ccactggggt ctttaccaat tcatcctggc tctggaacaa
     1921 actcttcgag gccagcattg aaacagggga ccgtgtctgg aggatgcctc tcttcgaaca
     1981 ttatacaaga caggttgtag attgccagct tgctgatgtt aacaacattg gaaaatacag
     2041 atctgcagga gcatgtacag ctgcagcatt cctgaaagaa ttcgtaactc atcctaagtg
     2101 ggcacattta gacatagcag gcgtgatgac caacaaagat gaagttccct atctacggaa
     2161 aggcatgact gggaggccca caaggactct cattgagttc ttacttcgtt tcagtcaaga
     2221 caatgcttag tacccagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc
     2281 aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc
     2341 tgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc
     2401 cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat
     2461 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga
     2521 aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc
     2581 tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc
     2641 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat
     2701 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg
     2761 tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt
     2821 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg
     2881 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct
     2941 cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga
     3001 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc
     3061 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg
     3121 gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct
     3181 tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca
     3241 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga
     3301 gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg
     3361 acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg
     3421 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3481 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3541 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3601 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3661 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3721 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     3781 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     3841 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     3901 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     3961 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     4021 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     4081 cttttgctgg ccttttgctc acatgtt
//
