LOCUS       sequence-451137-        3491 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  spisPink chromoprotein in a MultiGreen 1.0 HG entry vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Pennetti VJ, LaFayette PR, Parrott WA
  TITLE     MultiGreen: A multiplexing architecture for GreenGate cloning.
  JOURNAL   PLoS One. 2024 Sep 18;19(9):e0306008. doi: 
            10.1371/journal.pone.0306008. eCollection 2024.
  PUBMED    39292669
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2024-09-18"; volume: "19(9):e0306008. doi"; pages: " 
            10.1371/journal.pone.0306008. eCollection 2024"
COMMENT     Sequence Label: pVP085
FEATURES             Location/Qualifiers
     source          1..3491
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    16..37
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        38..72
                     /label=BBa_J23110
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.33"
     misc_feature    778..798
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(830..849)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(831..849)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(859..881)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1075..1094)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1194..1216
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1254..1272)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1340..1444
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1445..1513,1514..2305)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1663..1682)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2476..3064
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2965..2984
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3218..3235
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3352..3373
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3388..3393,3394..3411,3412..3418)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3426..3442
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        3456..3474
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3456..3473
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 taggacctag agacggaatt cgcggccgct tctagagttt acggctagct cagtcctagg
       61 tacaatgcta gctactagag atttaatagg aggtagttga tgtcgcactc aaaacaagca
      121 ctggcagata cgatgaaaat gacgtggctg atggaaggtt cggtcaacgg tcacgccttt
      181 acgattgagg gcgaaggtac cggcaaaccg tatgaaggta aacagagtgg cacctttcgt
      241 gttacgaaag gcggtccgct gccgtttgcg tttgatattg tcgctccgac cctgaaatac
      301 ggtttcaaat gcttcatgaa atacccggcg gatattccgg actactttaa actggccttc
      361 ccggaaggcc tgacgtacga tcgtaaaatt gcgtttgaag acggcggttg tgcgaccgcc
      421 acggtcgaaa tgagcctgaa gggtaacacc ctggtgcata aaacgaactt tcagggcggt
      481 aatttcccga ttgatggtcc ggtgatgcaa aaacgcaccc tgggctggga accgacctct
      541 gaaaaaatga cgccgtgcga tggtattatc aaaggcgaca ccatcatgta cctgatggtt
      601 gaaggcggta aaacgctgaa atgtcgttat gaaaacaatt accgcgccaa caaaccagtg
      661 ctgatgccgc cgagccactt tgtggatctg cgtctgaccc gcacgaatct ggataaagaa
      721 ggtctggcgt tcaaactgga agaatatgct gttgcccgtg tgctggaagt gtaataatac
      781 tagtagcggc cgctgcagcg tctctgtatg tattgagacc gaattctcgc cctatagtga
      841 gtcgtattac ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
      901 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
      961 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta
     1021 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
     1081 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     1141 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     1201 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     1261 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     1321 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     1381 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     1441 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     1501 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     1561 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     1621 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     1681 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     1741 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     1801 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     1861 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     1921 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     1981 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     2041 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     2101 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     2161 ctctcgaggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     2221 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     2281 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     2341 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     2401 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     2461 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     2521 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     2581 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     2641 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     2701 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     2761 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     2821 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     2881 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     2941 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     3001 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     3061 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     3121 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     3181 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     3241 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     3301 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     3361 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     3421 tggaattgtg agcggataac aatttcacac gagctattta ggtgacacta tagaagtgaa
     3481 gcttggtctc a
//
