LOCUS       sequence-45121-e         706 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Kim do J, Kim O, Kim HW, Kim HS, Lee SJ, Suh SW
  TITLE     ATP-dependent DNA ligase from Archaeoglobus fulgidus displays a 
            tightly closed conformation.
  JOURNAL   Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Jun 1. 65(Pt 
            6):544-50.
  PUBMED    19478428
REFERENCE   2  (bases 1 to 706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Acta 
            Crystallogr Sect F Struct Biol Cryst Commun. 2009 Jun 1. 65(Pt 
            6):544-50."
COMMENT     Sequence Label: pET28b-AF0623
FEATURES             Location/Qualifiers
     source          1..706
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagttattg
       61 tctctttacc tcgaactgta gcttataaag gttctcaacc ctctctatcg tatccgcctc
      121 gtcaacatcc ttatcatccc tcagtctgac gaacctgggg aatctcagcg cataaccgct
      181 ctcgtatttc gggcttttct gaatctcctg atacgcaacc tcgaaaacgt acttcgggat
      241 gaactccacc ttcttcccct gctgcgagac gatgaggggt ctgaacatct ctgtcagctc
      301 ctccagatcc tcctcagtga agcccgtggc gacccttcct accttaagca gtttgcccgt
      361 taccggatca aggcaggcga gctcaaagga gcttaaccag tgactccttt ttccctctcc
      421 ccactctcct ccaaccacaa cgaggtcgag agtttccatt atcgccttga ccttaagcca
      481 gttcttgccc ctcttccccg gaatgtaggg ggatgagggt agcttgatca tcactccctc
      541 gtgccctgcg ctgatagcct catcgtacat ctttcttacc tcatcaacac tgtccgtaac
      601 tatctgcttc gccagtttga ttttctccga ctcatttact gcggattcca gtagctttct
      661 cctctccctc agcggaaggt ctatgcactc tccatcgtgg tagaga
//
