LOCUS       sequence-451255-        6727 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV plasmid with Ple391 (DRD2 MiniPromoter) driving expression of 
            EmGFP. Contains WPRE..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6727)
  AUTHORS   de Moura Gomes A, L Petkau T, J Korecki A, Fornes O, Galvan A, Lu G,
            M Hill A, Ling Lam S, Yao A, A Farkas R, W Wasserman W, Smith Y, M 
            Simpson E, R Leavitt B
  TITLE     New MiniPromoter Ple389 (ADORA2A) drives selective expression in 
            medium spiny neurons in mice and non-human primates.
  JOURNAL   Sci Rep. 2024 Nov 15;14(1):28194. doi: 10.1038/s41598-024-79004-y.
  PUBMED    39548191
REFERENCE   2  (bases 1 to 6727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2024-11-15"; volume: "14(1):28194. doi"; pages: " 
            10.1038/s41598-024-79004-y"
COMMENT     Sequence Label: pEMS2389
FEATURES             Location/Qualifiers
     source          1..6727
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   12..141
                     /label=AAV2 ITR
     intron          1977..2109
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     2092..2111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      2130..2139
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2136..2138,2139..2141,2142..2855)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2181..2202)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2442..2461)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2789..2810
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2921..2941)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3339..3350)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3471..3592
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3482..3501)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3536..3555
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   3788..3917
                     /label=AAV2 ITR
     primer_bind     complement(3936..3953)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3936..3952)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3945..3967)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4094..4549
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4181..4200)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4391..4412
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4575..4679
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4680..4748,4749..5540)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4898..4917)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5711..6299
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6200..6219
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    6587..6608
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6623..6628,6629..6646,6647..6653)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6661..6677
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6666..6688
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6685..6701
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6685..6701
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ccttaattag gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc ttgtagttaa tgattaaccc gccatgctac ttatctacgt
      181 agccatgctc taggaagatc ggaattccct aggccggcca aggcagcagg acaggcagct
      241 gatgggcagg cctaggtaaa ggctctgact ctcaaggttg caaggttgcc caagtttcct
      301 gcagtctctt tacctggcat ctaggtggca gggggcaggg gtgtgagaag gcagaaaggg
      361 cactttagaa gcaaggaagt tagagccacc aagcatgggc tgggggctgg gctgaccttt
      421 cctttccttt agctgtgacg tgagctctag aaatagaggc aggaaggaag gagggtgaag
      481 ggcaggggcg ggaggcgggg caggggaaag ttgcccacag ttgtcacgag gcttcatgtc
      541 tttctgccag acagcagatt gacagctaga gtgggcaggg ggagggctgg gctccaccct
      601 ctccccctct cagcacttca ggggcaaaga aatgggagga gtaggacccg acacgacacg
      661 ggaacacaag gtggaagggg gtggcccagg ctctgactct ctgtcattgg aggaaatgct
      721 gagatgaagt taatccttgt ggcccagaga ggctggattc cagccatgca gggccgactc
      781 cctgctctgc agagtcagtc ctctgcccct gctcttccct ctccctggag tgttgtgcca
      841 ccttctcccc ttgggaactg gtgttgctcc ttcaatgccc ggttcctgta aagtctcgct
      901 tttttgtgct tcacagcacc tgtttaagcc tcagttattt ccctgggggt cccctctctg
      961 gattgccagg gtgcgggatc aggggcctgg ttttctttct gtgtgtgact tctgaatctg
     1021 acacagaatc tggcaaactg aggacagttg gtaactaatt gctgcagaag aaagaaaaga
     1081 aagagaaaga aagaaaaaga aaggaaggaa gaaagaaaga aagaaaggaa agaaagaaag
     1141 agaaagaaag aaagaaagaa agaaagaaag aaagaaagaa agaaagaaag aaagaaaaga
     1201 aaagaaaaga aagaaaagga gagaggaaga aaaggggaga agaacgaggt tcctctgcag
     1261 cccaaatgac agtgggcagg gtagcagcgg aacacccttc accactctat ctctttctcc
     1321 ctctccccac tccctgcctc acacacctca gtttggagtc aagggtgagc tggaacccct
     1381 gaggcctcct tttgcatttc catgtcaggt caactccaaa caggcttttt cctttggatg
     1441 agtccagcaa tagaactgac tacatagatt ccaagacctg aagtcagaaa acggatgcgg
     1501 acctcttcca acacctcctc tccttgcctg acttgctgga tgggtggtgc ctcccgcaac
     1561 ccttggcttc tgagtcctca aaggagaaga ctggcgagca gacggtgagg acccagcctg
     1621 caatcacagc ttattactct gggtgtgggt gggagcgcag tgggcggtcg agggttgcgt
     1681 tcccgcctca aaacaaggga tggcggaatc ccccaacccc tcctacccgt tcaggccggg
     1741 gatcgccgag gaggtacagc tcctttggtg gggggcgggg gcggggcctg tctcaggggc
     1801 ggggaccggg gcacctccct cccgcgctcc ccgcgctcgg gcgccgcaga gctgtccagc
     1861 ttcagtgccg aaccggcagc ctcacgcgcg caccgcgccg cctccgcccc gtccccgcgc
     1921 tccctcctgc ccgcccgccc cgggcgcgcc gaagttggtc gtgaggcact gggcaggtaa
     1981 gtatcaaggt tacaagacag gtttaaggag accaatagaa actgggcttg tcgagacaga
     2041 gaagactctt gcgtttctga taggcaccta ttggtcttac tgacatccac tttgcctttc
     2101 tctccacagg tgtccactcc caggcggccg ccaccatggt gagcaagggc gaggagctgt
     2161 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     2221 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     2281 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc acctacggcg
     2341 tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     2401 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     2461 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     2521 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     2581 acaaggtcta tatcaccgcc gacaagcaga agaacggcat caaggtgaac ttcaagaccc
     2641 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     2701 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
     2761 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     2821 ggatcactct cggcatggac gagctgtaca agtaagcggc cgcgatcgct caacctctgg
     2881 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     2941 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     3001 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     3061 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     3121 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     3181 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     3241 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     3301 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     3361 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     3421 agacgagtcg gatctccctt tgggccgcct ccccgcgatc gcagacatga taagatacat
     3481 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     3541 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     3601 caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt tttaaagcaa
     3661 gtaaaacctc tacaaatgtg gtagtcgaaa ttcccgataa ggatcttcgt cgacgaattc
     3721 ccgataagga tcttcctaga gcatggctac gtagataagt agcatggcgg gttaatcatt
     3781 aactacaagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc
     3841 actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg
     3901 agcgagcgag cgcgcagcct taattaacct aattcactgg ccgtcgtttt acaacgtcgt
     3961 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     4021 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     4081 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     4141 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     4201 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     4261 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     4321 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     4381 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     4441 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     4501 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     4561 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     4621 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     4681 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     4741 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     4801 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     4861 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     4921 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     4981 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     5041 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     5101 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     5161 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     5221 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     5281 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     5341 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     5401 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     5461 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     5521 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     5581 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     5641 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     5701 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     5761 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     5821 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     5881 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     5941 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     6001 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     6061 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     6121 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     6181 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     6241 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     6301 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     6361 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     6421 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     6481 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     6541 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     6601 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     6661 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccagattta
     6721 attaagg
//
