LOCUS       sequence-451264-       13404 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For targeted tethering of the catalytic domain of mouse TET2 using 
            dCas13.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13404)
  AUTHORS   Zou Z, Dou X, Li Y, Zhang Z, Wang J, Gao B, Xiao Y, Wang Y, Zhao L, 
            Sun C, Liu Q, Yu X, Wang H, Hong J, Dai Q, Yang FC, Xu M, He C
  TITLE     RNA m(5)C oxidation by TET2 regulates chromatin state and 
            leukaemogenesis.
  JOURNAL   Nature. 2024 Oct 2. doi: 10.1038/s41586-024-07969-x.
  PUBMED    39358506
REFERENCE   2  (bases 1 to 13404)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2024 Oct 2. doi: 10.1038/s41586-024-07969-x."
COMMENT     Sequence Label: pB-3xFlag-dCas13-mTET2CD
FEATURES             Location/Qualifiers
     source          1..13404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        556..935
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    complement(951..984)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        1007..1506
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1042..1065)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     1439..1458
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             1525..2550
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             1525..2550
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     repeat_region   3032..3066
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(3084..3103)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3085..3103)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3110..3127)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3110..3126)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3119..3141)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3268..3723
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3355..3374)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3565..3586
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3749..3853
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3854..3922,3923..4714)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4072..4091)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4885..5473
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5374..5393
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5627..5644
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5761..5782
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5797..5802,5803..5820,5821..5827)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5835..5851
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5859..5875
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5896..5914
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   5938..6000
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    complement(6171..6204)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    6281..6505
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     6494..6511
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     protein_bind    6571..6592
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6607..6612,6613..6630,6631..6637)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6645..6661
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6669..6685
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6706..6730
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(12323..12343)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(12368..12433)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(12442..12466)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(12517..12536)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          join(12544..13404,1..155)
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(12598..12620)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 agccgtcatt aaaccaagcg ctaattacag cccggaggag aagggccgtc ccgcccgctc
       61 acctgtggga gtaacgcggt cagtcagagc cggggcgggc ggcgcgaggc ggcggcggag
      121 cggggcacgg ggcgaaggca gcgcgcagcg actcccgccc gccgcgcgct tcgcttttta
      181 tagggccgcc gccgccgccg cctcgccata aaaggaaact ttcggagcgc gccgctctga
      241 ttggctgccg ccgcacctct ccgcctcgcc ccgccccgcc cctcgccccg ccccgccccc
      301 ccccgcgcgc gccccccccc cccccccaaa aaaaaaaaca cttcgcgggg cccccccccc
      361 gggcgggccc cccccccccc cccccccccc cccccccccc cccccccccc cccccccccc
      421 ccccccgggc ccgccccccc ccccccccgc ccccatcgct gcacaaaata attaaaaaat
      481 aaataaatac aaaattgggg gtggggaggg gggggagatg gggagagtga agcagaacgt
      541 ggggctcacc tcgaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg
      601 aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac
      661 gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta
      721 ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac
      781 atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt
      841 taccgtaagt tatgtaacgc ggaactccat atatgggcta tgaactaatg accccgtaat
      901 tgattactat taataactag tcaataatca atgtcgacct cgagggccgc ataacttcgt
      961 atagcataca ttatacgaag ttatgatatc atcgatgaat tctaccgggt aggggaggcg
     1021 cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac
     1081 acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg
     1141 ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc
     1201 gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc
     1261 gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa
     1321 tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc
     1381 gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca
     1441 ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc
     1501 ctttcgaccg atccagccgc caccatgaaa aagcctgaac tcaccgcgac gtctgtcgag
     1561 aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa
     1621 gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc
     1681 tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc
     1741 ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc
     1801 cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg
     1861 cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg
     1921 ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc
     1981 gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg
     2041 tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg
     2101 cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca
     2161 gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc
     2221 ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg
     2281 catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac
     2341 caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga
     2401 tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga
     2461 agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc
     2521 cccagcactc gtccgagggc aaaggaatag tcgagaaatt gatgatctat taaacaataa
     2581 agatgtccac atggaagttt ttcctgtcat actttgttaa gaagggtgag aacagagtac
     2641 ctacattttg aatggaagga ttggagctac gggggtgggg gtggggtggg attagataaa
     2701 tgcctgctct ttactgaagg ctctttacta ttgctttatg ataatgtttc atagttggat
     2761 atctataaca agaaaatata tatataataa gttatcacgt aagtagaaca tgaaataaca
     2821 atataattat cgtatgagtt aaatcttaaa agtcacgtaa aagataatca tgcgtcattt
     2881 tgactcacgc ggtcgttata gttcaaaatc agtgacactt accgcattga caagcacgcc
     2941 tcacgggagc tccaagcggc gactgagatg tcctaaatgc acagcgacgg attcgcgcta
     3001 tttagaaaga gagagcaata tttcaagaat gcatgcgtca attttacgca gactatcttt
     3061 ctagggttaa ggtacccaat tcgccctata gtgagtcgta ttacaattca ctggccgtcg
     3121 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     3181 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     3241 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     3301 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     3361 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     3421 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     3481 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     3541 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     3601 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     3661 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     3721 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     3781 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     3841 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     3901 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     3961 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     4021 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     4081 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     4141 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     4201 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     4261 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     4321 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     4381 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     4441 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     4501 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     4561 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     4621 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     4681 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     4741 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     4801 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     4861 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     4921 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     4981 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     5041 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     5101 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     5161 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     5221 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     5281 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     5341 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     5401 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     5461 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     5521 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     5581 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     5641 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     5701 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     5761 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     5821 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     5881 ttacgccaag ctcgaaatta accctcacta aagggaacaa aagctggagc tctttaaccc
     5941 tagaaagata atcatattgt gacgtacgtt aaagataatc atgcgtaaaa ttgacgcatg
     6001 tgttttatcg gtctgtatat cgaggtttat ttattaattt gaatagatat taagttttat
     6061 tatatttaca cttacatact aataataaat tcaacaaaca atttatttat gtttatttat
     6121 ttattaaaaa aaaacaaaaa ctcaaaattt cttctataaa gtaacaaaac ataacttcgt
     6181 atagcataca ttatacgaag ttatctcgac gcggccgctc tagcctcgag gctagaacta
     6241 gtggatctcg agccccagct ggttctttcc gcctcagaag ccatagagcc caccgcatcc
     6301 ccagcatgcc tgctattgtc ttcccaatcc tcccccttgc tgtcctgccc caccccaccc
     6361 cccagaatag aatgacacct actcagacaa tgcgatgcaa tttcctcatt ttattaggaa
     6421 aggacagtgg gagtggcacc ttccagggtc aaggaaggca cgggggaggg gcaaacaaca
     6481 gatggctggc aactagaagg cacagtcgag gctgatccgc tggcacgaca ggtttcccga
     6541 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     6601 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     6661 atttcacaca ggaaacagct atgaccatga ttacgccaag ctatcaccac tttgtacaag
     6721 aaagctgggt cttaattaat catacaaatg tgttgtaagg ccctgtgacc tgagtgaaag
     6781 catatggtga tgtagtcacg gtagaatccg tagtcacaga ccctgtgttc tcagcaagag
     6841 actggatgaa acgcaggtaa ctgggttcct gtggccctgt gggctcacgc ttttcctgtt
     6901 tgccatgatt tttctgagac acgtggtctg atccattctt tccgcactct tcctctttcc
     6961 gggccttttc agccattttg gcttcccaga gagccaaaca atgttttggt agaaacaaat
     7021 tcttatgcct atacagtaca agtgagatcc tggtggggtg attccggtcg ggatcgttta
     7081 ctttggttgt ggcatgaacc tcacactttg cacactcaat aagaattgac ccatgagttg
     7141 gagctatagc cacccctcca atgcaaggat cctgaaagtt gtgctcacta tctgaccaat
     7201 actcaatttc ctgcacagca gctgcatcct ggcctgacac ctcaggctgc ttctcctgac
     7261 tgctgccagt cagactgtat aatagttctt gggcagaagc agttctatca tgattaaacc
     7321 ctggaagcac tcttgagagc ccattggcta tgttgtcatt ctccttgttg tggaaggcgt
     7381 ggctggaact gctccccgaa gctgctgccg tgtagctgta gatcgtgtga gagggtagat
     7441 gatgctcact ggttttgtag tcagtgtgtg ggtggctgta tggtgatctg gaggcagctc
     7501 ccatgaaatg actatccatc ttgggggtgg ggtaaggaga aaacgttgct aggtggtgta
     7561 catttggttt tagggtggag ttagtagtga aggcatctct ctgcatattt tggtttccat
     7621 aacttaagta cttagaggga ctgtccccaa acgtctgttg gtgaagggtg tggatgggtg
     7681 gtaagttctg attggtgaga tggtcctggt ttggatatct atgtagatcc ctggactgag
     7741 cctgggggga ataagaacct aagaaagggg aaccattgtc cactggcaca ctcccattgt
     7801 atggaaaagg tgcatattgg ttattctgat tcaaaagccc aaggtagggg ttcatgtaat
     7861 tagaaggatt caaatatgaa cccgaggcat gagatgaagt ggggtaaaag ttcacatggt
     7921 tggtatctcc ataaatatct gaggtgtgtg ctgagctggg ataaggactg tgaggagtag
     7981 gctgtctcgt gtagacactg gtggatccag aacaatgaga accaacagac tgagagttac
     8041 cgggcatgat atgctgtggc tgtggctgtg gctgaggtgt cgtctgcggc tgcggctgag
     8101 gtggctgcgg ctgctgtggc ctctgccctt gctgaaggtg cctctggagt gttggtggct
     8161 gccggatgac cgggcccgaa agctgcggtt gtgctgtcat ttgtttcata tggcttgcat
     8221 tttccatcag ctttgtatgt gaggaagact ttcccttctc agtcctactg gagcagttct
     8281 ccagagagga acgctttcga gctgctttct tggtcttggc tttcttgggc tccgctttct
     8341 tcttgcaact cttgggcagc tctcctatcc ttatgaccct tctcctccga aatgactgca
     8401 gaacctcaat ggaccccatc cgtatcttct tctcctggcc ttccgtactc ccaaactcat
     8461 cctcaggggc gatgatgtac ataggcagca cgtggaactg ctcatcctca ggcttagctc
     8521 cgacttctcg attgtcttct ctattgaggg tgaccaccac tgtactgcca tttggcatgt
     8581 tctgctggtc tctgtgggaa tgagcagaga agtccaaaca tgcagtgact cctgagaatg
     8641 gccggccttc cttcagaccc aaacagcagt ctggggcttg gtgttcaaat tcaacctgat
     8701 tattgtatgc atcgggtgca agcttcttgt atattggagc aatgacagta gccaggtttt
     8761 gcaaatgaga acctagtctc tcttcctctt ttggctcagc tccatgtagc ctaaatttcc
     8821 ttggtttctt gcttctggca aacttacatc cattatagta catgctccaa gaacaaccaa
     8881 aagaaaagga ggcaccacag gtctctggat tctcaccctg acaacaacag tttctagttt
     8941 cattctgaga acagcgacgg ttggtgcata tgccacactt gcccaagata tctgtaagtt
     9001 ctgagtagag ttctgatgcg agtagctttg ggattccgtc ccacaacatg atggcaatta
     9061 ccatcaccgc cgtctcacat gtgtgattag gtcgcactcg taccaaacac agtagtttct
     9121 cctcctcact cgatctccga tatacccatt tagcaatagg acatccctga gagctcttgc
     9181 cttctttacc agtatatatg actttttcaa tcctaatagc tttacccttc tctccatacc
     9241 tttcttccat gagtgttcta atagctgcca catcaggacc agctcctaga tgggtataat
     9301 aaggagcttc atctttgtct ggattgcatc cttcacattt gccgaattcg ctgccgtcgg
     9361 cggttctttt ggatccatcc tctttggaca cgatgtcgct gcccacgagt tccagcaggt
     9421 tgccgatcct cttgtcgcta gccagcacaa agaagtcgcc gtagttcttc agcttcatgc
     9481 cctcgctggt gatggtgtac ttcttgccgc ctttctcgaa ggtgaagctc atgggcatga
     9541 tctcgctcag gattcccttc tcggcgtcgg gcatgatttc tttcagtttg aacctctcgc
     9601 cgtcgaaatc ggccagttcg gtcagggtct ttttggccag cagaaacagc agggcatcct
     9661 gcactctgta gcgccggatc actttctcgc ttttctggta ctcgttccgg ctgttgctca
     9721 gccgcttatc caggattgtc tcgatctctt cgctgctggc gtttctcatc tgccggttgc
     9781 tgcggatctt gttgctggcc tgctttctgt accgctctgt tctggaggcc cgctctttcc
     9841 agaggccttc tctctcttcc acgctggtga agcagtgctg cagggagccc tttctgtcgt
     9901 actcgccctt aagcatgtcc atgtaccggt agttgcggtt ccactggtag aaggtctgga
     9961 agtcgtcgtc cagcactctc ttcatgtact cggcgatcag ataggtcacg ttggcattgt
    10021 tgaagtcgat gccttccatc tgtggcaggg acttcaggtg ggacttgatc tcattgtcga
    10081 acatctgtct gggcagttcc acgggcagat cctcgctgta gattctgccc agggtcttca
    10141 tggcgggtgt tttccacttg ttctggtccc gccggatgaa gggcacatcc actctgttgc
    10201 ctttcttgat ctcgttggac aggccggtca ggtagaactt ccgctcgatc aggtagcgct
    10261 cgtagaactc gacggcattg gcggggatgc tgcgggcgaa caccttgtac agaaatggat
    10321 gaggctctgt tgtgcccttg ccgatcagcc gggccttctc gaacatcagc ttgaactgct
    10381 gcttggcctc gtaatcgtcg ccgctatcgt acacggcaat ggcgctctgc atgatccggt
    10441 agttcaggcc ggtgatcttg ttctcgccat cgttcacgct gggctgaaac agcacgatgt
    10501 ccttggccag gaagtcggcc agcttgcctg tggagatctg cttgaagcct ctctttccca
    10561 tcttgttgtc ggcgctccga atggacttcc ggtcgtcctt gaatctcttg attctccgct
    10621 cggtgtcggt cagcatgtcg tccacggtca gtctgatgaa ggcgtccaca tccttgccgt
    10681 gggcattgcc gctgatcaga tccaggatct tctgaggcag gtcgctctcg gcgattccga
    10741 agctggcgat attctcggcg gtcacttctt ctttctgcat ggcctggaac agtctcttgt
    10801 accggttgtg cacgtccacg atcagcttct cggttttctt gctgccgaac agaaacatgt
    10861 ggaaggccat ggctggaatt tccagggtgc tcatccggca gctggggatt gtcttgacca
    10921 cgtatctatc atcctcgatc acgggcagca gtggggcgct gtcctctttg tcgttgataa
    10981 acatctcgac cttgttgttt tccaggatgt agtgtgtgta ggtgtccacg atgtagggat
    11041 agttggcagg attggcgtcg tcccgcttca tgttctcgaa gtctctgatc cggatgccgc
    11101 tgttgccgaa ggtgccgttc tcttgcttcc gcattgtctc ggcctcttcc agtctgccga
    11161 agccgttcag gggctgctcg atcactctga ctctggtctg gccgtcgatg caggtcttgt
    11221 cggccttcag caggtatctc agcttgccca tgttcacgtg gaacctgatg tggtcgaaca
    11281 gcttgccgta atcgatatac tgcagcagca gaggcacgaa tctgtcgctg ctccgcttca
    11341 tcagcacttc attgtggtcg tcgctgatga ttctgaaccg ggactgcttc tcggcagaca
    11401 gtgttgtgaa cagctcgtcg gggcaccgct tcacttcgtt gagcatatcc atggccacgc
    11461 tcttgttgga cttctcgctg tggatccggt ccttgggcag cttgatgctg ttgatgccga
    11521 aggatctgat gatgatccgc cgttcctcgc tctgggcatt gtagctggag aagatgggca
    11581 gcctgctcag aaagatgttg atgtactgct tgtccaggaa caggcagatc agcagggcga
    11641 ttcccactcc gctcaggtgc agcttcttct gtgtgtcgcc gttgtagtcc tgcaggctca
    11701 ggaagaatcc ggtattcact tgggactttt tcttgccgta ggcgtccttc acgaacttga
    11761 accgcttgtc ctggatgaag gccaggtcct ctgtcttgta gccgtatctc tcgttcatgt
    11821 tccgcagggc cactgtgtag tagttgttga tcatgccgct cagaggttgc tctgtgctgg
    11881 tcaggaactc gcagccgtcg ttcagctttt cctcgtaggt cttgtatgcg ttggtcaggt
    11941 ccctgtacat cttcagcacg ccgaaggcgc gcttcagcac ctcgaagatg tcgttgctgt
    12001 tcacttccac gcggttctgc ttgtacttgc cgttgctgta ctctctctgg ttctcggcca
    12061 tgatcttcag gaatgggaag tagctctgca gccgctcgat gatgaacatg gttttctcgg
    12121 gctgcttgtc gtagccgttc ttggcgttgt acaggtggct catcacgggg tgaaaccaca
    12181 gattctcgtt gttctcgttc tgctcgccct caatatcggc caccttctgg atgtggtcca
    12241 gcacggtctg agcgttcagc atggccatca cgctgtaggt gccaaagtac ttcttctggt
    12301 tttccaccag agcggggatg ttgactttcc gcttcttctt tgggctagcg gcgccgccgc
    12361 cggatccctt gtcatcgtca tccttgtaat cgatatcatg atctttataa tcaccgtcat
    12421 ggtctttgta gtccatggtg aagcctgctt ttttgtacaa acttgtgata gcttatcgat
    12481 accgtcgagg aattctttgc caaaatgatg agacagcaca ataaccagca cgttgcccag
    12541 gagctgtagg aaaaagaaga aggcatgaac atggttagca gaggctctag agccgccggt
    12601 cacacgccag aagccgaacc ccgccctgcc ccgtcccccc cgaaggcagc cgtccccccg
    12661 cggacagccc cgaggctgga gagggagaag gggacggcgg cgcggcgacg cacgaaggcc
    12721 ctccccgccc atttccttcc tgccggcgcc gcaccgcttc gccccgcgcc cgctagaggg
    12781 ggtgcggcgg cgcctcccag atttcggctc cgcacagatt tgggacaaag gaagtccctg
    12841 cgccctctcg cacgattacc ataaaaggca atggctgcgg ctcgccgcgc ctcgacagcc
    12901 gccggcgctc cgggggccgc cgcgcccctc ccccgagccc tccccggccc gaggcggccc
    12961 cgccccgccc ggcaccccca cctgccgcca ccccccgccc ggcacggcga gccccgcgcc
    13021 acgccccgta cggagccccg cacccgaagc cgggccgtgc tcagcaactc ggggaggggg
    13081 gtgcaggggg ggttgcagcc cgaccgacgc gcccacaccc cctgctcacc cccccacgca
    13141 cacaccccgc acgcagcctt tgttcccctc gcagcccccc cgcaccgcgg ggcaccgccc
    13201 ccggccgcgc tcccctcgcg cacactgcgg agcgcacaaa gccccgcgcc gcgcccgcag
    13261 cgctcacagc cgccgggcag cgcggagccg cacgcggcgc tccccacgca cacacacacg
    13321 cacgcacccc ccgagccgct ccccccgcac aaagggccct cccggagccc ctcaaggctt
    13381 tcacgcagcc acagaaaaga aaca
//
